(Circular) (Six-base) MAPSORT of: pFN21AB0491.seq Check: 7329 from: 1 to: 6484 pFN21AB0491 6484 bp With 182 enzymes: SgfI * April 15, 2010 09:33 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2552 2552 Size: 6484 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4294 278 Size: 53 83 186 936 2758 2468 Fragments arranged by size: 2758 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3827 1937 Size: 1890 4594 Acc65I G'GTAC_C Cuts at: 3805 3805 Size: 6484 AclI AA'CG_TT Cuts at: 1563 3783 4611 4984 1563 Size: 2220 828 373 3063 Fragments arranged by size: 3063 2220 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2896 4547 5931 6199 1632 Size: 1264 1651 1384 268 1917 Fragments arranged by size: 1917 1651 1384 1264 268 AfeI AGC'GCT Cuts at: 3307 3307 Size: 6484 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6287 AflIII A'CryG_T Cuts at: 1203 5383 6360 1203 Size: 4180 977 1327 Fragments arranged by size: 4180 1327 977 AgeI A'CCGG_T Cuts at: 5315 5315 Size: 6484 AhdI GACnn_n'nnGTC Cuts at: 2341 5213 2341 Size: 2872 3612 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6484 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3490 1184 Size: 2306 4178 AlwNI CAG_nnn'CTG Cuts at: 2224 2395 3178 4227 5799 2224 Size: 171 783 1049 1572 2909 Fragments arranged by size: 2909 1572 1049 783 171 ApaI G_GGCC'C Cuts at: 2782 2782 Size: 6484 ApaLI G'TGCA_C Cuts at: 4541 5697 4541 Size: 1156 5328 ApoI r'AATT_y Cuts at: 1493 3793 4019 4171 6280 1493 Size: 2300 226 152 2109 1697 Fragments arranged by size: 2300 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5038 160 Size: 872 4006 1606 Fragments arranged by size: 4006 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6484 AvaI C'yCGr_G Cuts at: 1870 1943 2078 2087 3233 3370 3575 3614 3724 Size: 73 135 9 1146 137 205 39 110 Cuts at: 3724 3809 1870 Size: 85 4545 Fragments arranged by size: 4545 1146 205 137 135 110 85 73 39 9 BamHI G'GATC_C Cuts at: 3814 4181 3814 Size: 367 6117 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3284 3805 5260 618 Size: 325 217 627 1497 521 1455 1842 Fragments arranged by size: 1842 1497 1455 627 521 325 217 BanII G_rGCy'C Cuts at: 1391 2361 2782 2882 3003 3474 3615 3650 3803 Size: 970 421 100 121 471 141 35 153 Cuts at: 3803 1391 Size: 4072 Fragments arranged by size: 4072 970 471 421 153 141 121 100 35 BbeI G_GCGC'C Cuts at: 3288 3288 Size: 6484 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5513 BbvCI CC'TCA_GC Cuts at: 3689 3689 Size: 6484 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2276 2310 4675 4709 1903 Size: 34 339 34 2365 34 3678 Fragments arranged by size: 3678 2365 339 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4376 5586 4376 Size: 1210 5274 BclI T'GATC_A Cuts at: 1548 1548 Size: 6484 BfrBI ATG'CAT Cuts at: 3673 3673 Size: 6484 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2828 2840 3071 Size: 107 122 71 631 605 1156 12 231 Cuts at: 3071 3095 5095 136 Size: 24 2000 1525 Fragments arranged by size: 2000 1525 1156 631 605 231 122 107 71 24 12 BglII A'GATC_T Cuts at: 6479 6479 Size: 6484 BlpI GC'TnA_GC Cuts at: 2279 3731 3901 2279 Size: 1452 170 4862 Fragments arranged by size: 4862 1452 170 Bme1580I G_kGCm'C Cuts at: 1790 2782 4545 5701 1790 Size: 992 1763 1156 2573 Fragments arranged by size: 2573 1763 1156 992 BmgBI CAC'GTC Cuts at: 1318 2764 2892 2922 1318 Size: 1446 128 30 4880 Fragments arranged by size: 4880 1446 128 30 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4215 5173 442 Size: 416 53 83 399 24 2798 958 1753 Fragments arranged by size: 2798 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6484 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2119 2461 3664 2119 Size: 342 1203 4939 Fragments arranged by size: 4939 1203 342 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2141 2174 2246 2585 2890 3104 5144 1239 Size: 902 33 72 339 305 214 2040 2579 Fragments arranged by size: 2579 2040 902 339 305 214 72 33 Bpu10I CC'TnA_GC Cuts at: 3266 3500 3609 3689 3266 Size: 234 109 80 6061 Fragments arranged by size: 6061 234 109 80 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4609 5474 5772 4609 Size: 865 298 5321 Fragments arranged by size: 5321 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2084 2721 3131 5147 882 Size: 617 585 637 410 2016 2219 Fragments arranged by size: 2219 2016 637 617 585 410 BsaAI yAC'GTr Cuts at: 493 1204 4275 493 Size: 711 3071 2702 Fragments arranged by size: 3071 2702 711 BsaBI GATnn'nnATC Cuts at: 6478 6478 Size: 6484 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3259 3285 4291 4673 Size: 53 83 186 936 1726 26 1006 382 Cuts at: 4673 5308 275 Size: 635 1451 Fragments arranged by size: 1726 1451 1006 936 635 382 186 83 53 26 BsaWI w'CCGG_w Cuts at: 4917 5315 5589 5736 6147 4917 Size: 398 274 147 411 5254 Fragments arranged by size: 5254 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3416 3434 3446 3464 1178 Size: 30 165 30 2013 18 12 18 4198 Fragments arranged by size: 4198 2013 165 30 30 18 18 12 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2276 2279 2729 2822 2825 2828 2831 2942 Size: 1090 3 450 93 3 3 3 111 Cuts at: 2942 1186 Size: 4728 Fragments arranged by size: 4728 1090 450 111 93 3 3 3 3 BseYI C'CCAG_C Cuts at: 2388 2867 3168 3548 3696 3759 5687 6183 2388 Size: 479 301 380 148 63 1928 496 2689 Fragments arranged by size: 2689 1928 496 479 380 301 148 63 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2422 2456 3602 1163 Size: 573 686 34 1146 4045 Fragments arranged by size: 4045 1146 686 573 34 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2805 3010 3066 3135 3316 3391 Size: 275 186 804 205 56 69 181 75 Cuts at: 3391 3517 3934 4695 5723 1540 Size: 126 417 761 1028 2301 Fragments arranged by size: 2301 1028 804 761 417 275 205 186 181 126 75 69 56 BsiHKAI G_wGCw'C Cuts at: 2361 2940 3003 3650 4545 4630 5701 2361 Size: 579 63 647 895 85 1071 3144 Fragments arranged by size: 3144 1071 895 647 579 85 63 BsmI GAATG_Cn' Cuts at: 3995 4088 3995 Size: 93 6391 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2361 2782 2882 2940 3003 3474 3615 Size: 399 571 421 100 58 63 471 141 Cuts at: 3615 3650 3803 4545 4630 5701 1391 Size: 35 153 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 571 471 421 399 153 141 100 85 63 58 35 BspEI T'CCGG_A Cuts at: 6147 6147 Size: 6484 BspHI T'CATG_A Cuts at: 4373 6103 4373 Size: 1730 4754 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2552 3839 844 Size: 1708 1287 3489 Fragments arranged by size: 3489 1708 1287 BsrBI CCG'CTC Cuts at: 1394 1823 2509 2719 3403 3519 4371 1394 Size: 429 686 210 684 116 852 3507 Fragments arranged by size: 3507 852 686 684 429 210 116 BsrDI GCAATG_nn' Cuts at: 65 1248 2550 4979 5153 65 Size: 1183 1302 2429 174 1396 Fragments arranged by size: 2429 1396 1302 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2730 3311 5128 5315 1700 Size: 110 920 581 1817 187 2869 Fragments arranged by size: 2869 1817 920 581 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6484 BssHII G'CGCG_C Cuts at: 1925 2019 2137 2139 1925 Size: 94 118 2 6270 Fragments arranged by size: 6270 118 94 2 BssSI C'ACGA_G Cuts at: 2285 4544 5556 2285 Size: 2259 1012 3213 Fragments arranged by size: 3213 2259 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6484 BstBI TT'CG_AA Cuts at: 5288 5288 Size: 6484 BstEII G'GTnAC_C Cuts at: 1184 2326 1184 Size: 1142 5342 BstXI CCAn_nnnn'nTGG Cuts at: 2563 3195 2563 Size: 632 5852 BstYI r'GATC_y Cuts at: 1972 3814 4181 4566 4583 6035 6121 6133 6479 Size: 1842 367 385 17 1452 86 12 346 Cuts at: 6479 1972 Size: 1977 Fragments arranged by size: 1977 1842 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2657 3060 3066 3651 3710 513 Size: 552 939 653 403 6 585 59 3287 Fragments arranged by size: 3287 939 653 585 552 403 59 6 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5745 Fragments arranged by size: 5745 683 56 BtsI GCAGTG_nn' Cuts at: 2201 2405 2695 3530 4000 4794 4814 2201 Size: 204 290 835 470 794 20 3871 Fragments arranged by size: 3871 835 794 470 290 204 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6484 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6449 DraI TTT'AAA Cuts at: 3788 3788 Size: 6484 DraIII CAC_nnn'GTG Cuts at: 2431 2431 Size: 6484 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5491 809 Size: 507 4175 1802 Fragments arranged by size: 4175 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2802 3063 3132 Size: 54 1425 50 275 18 972 261 69 Cuts at: 3132 3313 3388 3514 3931 4820 6449 8 Size: 181 75 126 417 889 1629 43 Fragments arranged by size: 1629 1425 972 889 417 275 261 181 126 75 69 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2802 3063 3132 3313 3388 3514 3931 Size: 275 990 261 69 181 75 126 417 Cuts at: 3931 1537 Size: 4090 Fragments arranged by size: 4090 990 417 275 261 181 126 75 69 EarI CTCTTCn'nnn_ Cuts at: 1353 3008 4414 1353 Size: 1655 1406 3423 Fragments arranged by size: 3423 1655 1406 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2602 3127 5071 5445 5591 387 Size: 1064 138 1013 525 1944 374 146 1280 Fragments arranged by size: 1944 1280 1064 1013 525 374 146 138 EcoICRI GAG'CTC Cuts at: 2359 3001 3648 2359 Size: 642 647 5195 Fragments arranged by size: 5195 647 642 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2141 2174 2246 2585 2890 2896 3104 Size: 393 509 33 72 339 305 6 208 Cuts at: 3104 4547 5144 5931 6199 1239 Size: 1443 597 787 268 1524 Fragments arranged by size: 1524 1443 787 597 509 393 339 305 268 208 72 33 6 EcoNI CCTnn'n_nnAGG Cuts at: 2416 3452 3752 2416 Size: 1036 300 5148 Fragments arranged by size: 5148 1036 300 EcoO109I rG'GnC_Cy Cuts at: 3164 3175 3164 Size: 11 6473 EcoRI G'AATT_C Cuts at: 3793 4171 3793 Size: 378 6106 FspI TGC'GCA Cuts at: 2048 2177 4990 2048 Size: 129 2813 3542 Fragments arranged by size: 3542 2813 129 HaeII r_GCGC'y Cuts at: 1678 2919 3288 3309 5631 1678 Size: 1241 369 21 2322 2531 Fragments arranged by size: 2531 2322 1241 369 21 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2327 2359 3416 3434 3448 Size: 32 135 32 755 32 1057 18 14 Cuts at: 3448 3466 3493 3525 5125 5157 5199 5231 1373 Size: 18 27 32 1600 32 42 32 2626 Fragments arranged by size: 2626 1600 1057 755 135 42 32 32 32 32 32 32 27 18 18 14 HincII GTy'rAC Cuts at: 669 1657 1938 3828 4074 669 Size: 988 281 1890 246 3079 Fragments arranged by size: 3079 1890 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6484 HpaI GTT'AAC Cuts at: 4074 4074 Size: 6484 KasI G'GCGC_C Cuts at: 3284 3284 Size: 6484 KpnI G_GTAC'C Cuts at: 3809 3809 Size: 6484 MfeI C'AATT_G Cuts at: 4083 4083 Size: 6484 MluI A'CGCG_T Cuts at: 6360 6360 Size: 6484 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2904 5598 5782 1305 Size: 1599 2694 184 2007 Fragments arranged by size: 2694 2007 1599 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4662 Fragments arranged by size: 4662 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4442 4801 4960 518 Size: 648 3276 359 159 2042 Fragments arranged by size: 3276 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2392 2659 3068 3082 3586 3712 3900 4577 Size: 786 267 409 14 504 126 188 677 Cuts at: 4577 5725 5970 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 786 677 504 409 267 245 188 126 14 NaeI GCC'GGC Cuts at: 3313 3313 Size: 6484 NarI GG'CG_CC Cuts at: 3285 3285 Size: 6484 NcoI C'CATG_G Cuts at: 513 1065 2004 3651 513 Size: 552 939 1647 3346 Fragments arranged by size: 3346 1647 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6484 NgoMIV G'CCGG_C Cuts at: 3311 3311 Size: 6484 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6484 NotI GC'GGCC_GC Cuts at: 3931 3931 Size: 6484 NsiI A_TGCA'T Cuts at: 3675 3675 Size: 6484 NspI r_CATG'y Cuts at: 1132 1229 3842 5387 1132 Size: 97 2613 1545 2229 Fragments arranged by size: 2613 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 3454 1348 Size: 2106 4378 PciI A'CATG_T Cuts at: 5383 5383 Size: 6484 PflMI CCAn_nnn'nTGG Cuts at: 1108 2119 2224 1108 Size: 1011 105 5368 Fragments arranged by size: 5368 1011 105 PfoI T'CCnGG_A Cuts at: 2454 2454 Size: 6484 PmeI GTTT'AAAC Cuts at: 3788 3788 Size: 6484 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2081 2113 4553 4585 6066 6098 2081 Size: 32 2440 32 1481 32 2467 Fragments arranged by size: 2467 2440 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3164 3164 Size: 6484 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6484 PsiI TTA'TAA Cuts at: 4054 4054 Size: 6484 PspOMI G'GGCC_C Cuts at: 2778 2778 Size: 6484 PstI C_TGCA'G Cuts at: 830 2028 2208 2400 2495 2690 3332 3836 830 Size: 1198 180 192 95 195 642 504 3478 Fragments arranged by size: 3478 1198 642 504 195 192 180 95 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6484 PvuII CAG'CTG Cuts at: 2392 3586 2392 Size: 1194 5290 SacI G_AGCT'C Cuts at: 2361 3003 3650 2361 Size: 642 647 5195 Fragments arranged by size: 5195 647 642 SacII CC_GC'GG Cuts at: 2660 3069 3713 2660 Size: 409 644 5431 Fragments arranged by size: 5431 644 409 SalI G'TCGA_C Cuts at: 1936 3826 1936 Size: 1890 4594 SanDI GG'GwC_CC Cuts at: 3164 3164 Size: 6484 SapI GCTCTTCn'nnn_ Cuts at: 3008 3008 Size: 6484 SbfI CC_TGCA'GG Cuts at: 3836 3836 Size: 6484 ScaI AGT'ACT Cuts at: 4732 6444 4732 Size: 1712 4772 SfcI C'TryA_G Cuts at: 826 1045 2024 2204 2362 2396 2491 2686 3328 Size: 219 979 180 158 34 95 195 642 Cuts at: 3328 3832 4967 5648 5839 826 Size: 504 1135 681 191 1471 Fragments arranged by size: 1471 1135 979 681 642 504 219 195 191 180 158 95 34 SfiI GGCCn_nnn'nGGCC Cuts at: 3095 3095 Size: 6484 SfoI GGC'GCC Cuts at: 3286 3286 Size: 6484 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6484 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6484 SmaI CCC'GGG Cuts at: 1872 3235 3372 3811 1872 Size: 1363 137 439 4545 Fragments arranged by size: 4545 1363 439 137 SmlI C'TyrA_G Cuts at: 820 1017 1943 2078 2087 4588 5489 5751 820 Size: 197 926 135 9 2501 901 262 1553 Fragments arranged by size: 2501 1553 926 901 262 197 135 9 SnaBI TAC'GTA Cuts at: 493 4275 493 Size: 3782 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6484 SphI G_CATG'C Cuts at: 1132 3842 1132 Size: 2710 3774 SrfI GCCC'GGGC Cuts at: 3372 3372 Size: 6484 SspI AAT'ATT Cuts at: 5 52 4408 5 Size: 47 4356 2081 Fragments arranged by size: 4356 2081 47 StuI AGG'CCT Cuts at: 1517 2861 3659 3704 1517 Size: 1344 798 45 4297 Fragments arranged by size: 4297 1344 798 45 StyI C'CwwG_G Cuts at: 513 1065 2004 2322 2556 3054 3636 3651 3753 Size: 552 939 318 234 498 582 15 102 Cuts at: 3753 3923 513 Size: 170 3074 Fragments arranged by size: 3074 939 582 552 498 318 234 170 102 15 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2108 2774 3562 4511 4528 4681 4866 Size: 1255 528 666 788 949 17 153 185 Cuts at: 4866 5311 325 Size: 445 1498 Fragments arranged by size: 1498 1255 949 788 666 528 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4730 6442 96 Size: 274 80 33 51 1443 2753 1712 138 Fragments arranged by size: 2753 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6444 6476 6444 Size: 32 6452 Tth111I GACn'n_nGTC Cuts at: 1316 2534 2962 3118 1316 Size: 1218 428 156 4682 Fragments arranged by size: 4682 1218 428 156 XbaI T'CTAG_A Cuts at: 3820 3820 Size: 6484 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3067 3634 3067 Size: 567 5917 XhoI C'TCGA_G Cuts at: 1943 2078 2087 1943 Size: 135 9 6340 Fragments arranged by size: 6340 135 9 XmaI C'CCGG_G Cuts at: 1870 3233 3370 3809 1870 Size: 1363 137 439 4545 Fragments arranged by size: 4545 1363 439 137 XmnI GAAnn'nnTTC Cuts at: 4613 4613 Size: 6484 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4292 276 Size: 53 83 186 936 2758 2468 Fragments arranged by size: 2758 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BsiWI BsmBI BstZ17I Bsu36I EcoRV FalI FseI FspAI NruI PacI PmlI PspXI PsrI RsrII SexAI SwaI