(Circular) (Six-base) MAPSORT of: pFN21AB0477.seq Check: 5080 from: 1 to: 6259 pFN21AB0477 6259 bp With 182 enzymes: SgfI * April 15, 2010 09:33 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2993 2993 Size: 6259 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4069 278 Size: 53 83 186 936 2533 2468 Fragments arranged by size: 2533 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3602 1937 Size: 1665 4594 Acc65I G'GTAC_C Cuts at: 3580 3580 Size: 6259 AclI AA'CG_TT Cuts at: 1563 4386 4759 1563 Size: 2823 373 3063 Fragments arranged by size: 3063 2823 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2528 2932 3110 4322 5706 5974 1632 Size: 896 404 178 1212 1384 268 1917 Fragments arranged by size: 1917 1384 1212 896 404 268 178 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6062 AflIII A'CryG_T Cuts at: 1203 2670 5158 6135 1203 Size: 1467 2488 977 1327 Fragments arranged by size: 2488 1467 1327 977 AgeI A'CCGG_T Cuts at: 2682 5090 2682 Size: 2408 3851 AhdI GACnn_n'nnGTC Cuts at: 4988 4988 Size: 6259 AleI CACnn'nnGTG Cuts at: 1166 3053 1166 Size: 1887 4372 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6259 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3180 3212 3180 Size: 32 6227 AlwNI CAG_nnn'CTG Cuts at: 2290 2416 2506 2985 3080 3106 4002 5574 2290 Size: 126 90 479 95 26 896 1572 2975 Fragments arranged by size: 2975 1572 896 479 126 95 90 26 ApaI G_GGCC'C Cuts at: 2362 2362 Size: 6259 ApaLI G'TGCA_C Cuts at: 4316 5472 4316 Size: 1156 5103 ApoI r'AATT_y Cuts at: 1493 2850 3568 3794 3946 6055 1493 Size: 1357 718 226 152 2109 1697 Fragments arranged by size: 2109 1697 1357 718 226 152 AseI AT'TA_AT Cuts at: 160 1032 4813 160 Size: 872 3781 1606 Fragments arranged by size: 3781 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6259 AvaI C'yCGr_G Cuts at: 1870 1943 2064 2562 2610 3021 3584 1870 Size: 73 121 498 48 411 563 4545 Fragments arranged by size: 4545 563 498 411 121 73 48 BamHI G'GATC_C Cuts at: 3589 3956 3589 Size: 367 5892 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2782 3580 5035 618 Size: 325 217 627 995 798 1455 1842 Fragments arranged by size: 1842 1455 995 798 627 325 217 BanII G_rGCy'C Cuts at: 1391 2232 2362 2713 3098 3578 1391 Size: 841 130 351 385 480 4072 Fragments arranged by size: 4072 841 480 385 351 130 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5288 BbvCI CC'TCA_GC Cuts at: 2172 2291 2172 Size: 119 6140 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4450 4484 1903 Size: 34 2513 34 3678 Fragments arranged by size: 3678 2513 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4151 5361 4151 Size: 1210 5049 BclI T'GATC_A Cuts at: 1548 1548 Size: 6259 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2366 4870 136 Size: 107 122 71 631 605 694 2504 1525 Fragments arranged by size: 2504 1525 694 631 605 122 107 71 BglII A'GATC_T Cuts at: 2881 3135 6254 2881 Size: 254 3119 2886 Fragments arranged by size: 3119 2886 254 BlpI GC'TnA_GC Cuts at: 3676 3676 Size: 6259 Bme1580I G_kGCm'C Cuts at: 1790 2362 2425 2541 3438 4320 5476 1790 Size: 572 63 116 897 882 1156 2573 Fragments arranged by size: 2573 1156 897 882 572 116 63 BmgBI CAC'GTC Cuts at: 1318 2536 1318 Size: 1218 5041 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3200 3990 4948 Size: 416 53 83 399 24 1783 790 958 Cuts at: 4948 442 Size: 1753 Fragments arranged by size: 1783 1753 958 790 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6259 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2266 2577 3112 2266 Size: 311 535 5413 Fragments arranged by size: 5413 535 311 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2246 2342 2727 2962 3107 3128 3419 3431 Size: 1007 96 385 235 145 21 291 12 Cuts at: 3431 3482 4919 1239 Size: 51 1437 2579 Fragments arranged by size: 2579 1437 1007 385 291 235 145 96 51 21 12 Bpu10I CC'TnA_GC Cuts at: 2172 2291 2172 Size: 119 6140 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2501 4384 5249 5547 2501 Size: 1883 865 298 3213 Fragments arranged by size: 3213 1883 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4922 882 Size: 617 3423 2219 Fragments arranged by size: 3423 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2426 2671 3490 4050 493 Size: 711 1222 245 819 560 2702 Fragments arranged by size: 2702 1222 819 711 560 245 BsaBI GATnn'nnATC Cuts at: 3134 6253 3134 Size: 3119 3140 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4066 4448 5083 275 Size: 53 83 186 936 2533 382 635 1451 Fragments arranged by size: 2533 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2124 2313 2439 2682 4692 5090 5364 5511 5922 Size: 189 126 243 2010 398 274 147 411 Cuts at: 5922 2124 Size: 2461 Fragments arranged by size: 2461 2010 411 398 274 243 189 147 126 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3104 3134 3180 3210 3323 Size: 30 165 30 1701 30 46 30 113 Cuts at: 3323 3353 1178 Size: 30 4084 Fragments arranged by size: 4084 1701 165 113 46 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2498 3125 1186 Size: 1312 627 4320 Fragments arranged by size: 4320 1312 627 BseYI C'CCAG_C Cuts at: 2475 3148 3326 5462 5958 2475 Size: 673 178 2136 496 2776 Fragments arranged by size: 2776 2136 673 496 178 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2411 3262 1163 Size: 573 675 851 4160 Fragments arranged by size: 4160 851 675 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3709 4470 5498 1540 Size: 275 186 1708 761 1028 2301 Fragments arranged by size: 2301 1708 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2232 2571 3098 3432 4320 4405 5476 2232 Size: 339 527 334 888 85 1071 3015 Fragments arranged by size: 3015 1071 888 527 339 334 85 BsmI GAATG_Cn' Cuts at: 3770 3863 3770 Size: 93 6166 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2232 2362 2425 2541 2571 2713 3098 Size: 399 442 130 63 116 30 142 385 Cuts at: 3098 3432 3438 3578 4320 4405 5476 1391 Size: 334 6 140 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 442 399 385 334 142 140 130 116 85 63 30 6 BspEI T'CCGG_A Cuts at: 2313 2439 5922 2313 Size: 126 3483 2650 Fragments arranged by size: 3483 2650 126 BspHI T'CATG_A Cuts at: 4148 5878 4148 Size: 1730 4529 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2268 2505 2703 2964 2993 3614 844 Size: 1424 237 198 261 29 621 3489 Fragments arranged by size: 3489 1424 621 261 237 198 29 BsrBI CCG'CTC Cuts at: 1394 1823 2188 4146 1394 Size: 429 365 1958 3507 Fragments arranged by size: 3507 1958 429 365 BsrDI GCAATG_nn' Cuts at: 65 1248 4754 4928 65 Size: 1183 3506 174 1396 Fragments arranged by size: 3506 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2682 4903 5090 1700 Size: 110 872 2221 187 2869 Fragments arranged by size: 2869 2221 872 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6259 BssHII G'CGCG_C Cuts at: 1925 2147 1925 Size: 222 6037 BssSI C'ACGA_G Cuts at: 3431 4319 5331 3431 Size: 888 1012 4359 Fragments arranged by size: 4359 1012 888 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6259 BstBI TT'CG_AA Cuts at: 5063 5063 Size: 6259 BstEII G'GTnAC_C Cuts at: 1184 3259 1184 Size: 2075 4184 BstXI CCAn_nnnn'nTGG Cuts at: 2381 2675 2381 Size: 294 5965 BstYI r'GATC_y Cuts at: 1972 2310 2436 2881 3135 3589 3956 4341 4358 Size: 338 126 445 254 454 367 385 17 Cuts at: 4358 5810 5896 5908 6254 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 454 445 385 367 346 338 254 126 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2016 2797 2940 513 Size: 552 939 12 781 143 3832 Fragments arranged by size: 3832 939 781 552 143 12 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3373 487 Size: 683 56 2147 3373 Fragments arranged by size: 3373 2147 683 56 BtsI GCAGTG_nn' Cuts at: 2358 2811 3775 4569 4589 2358 Size: 453 964 794 20 4028 Fragments arranged by size: 4028 964 794 453 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6259 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6224 DraI TTT'AAA Cuts at: 3563 3563 Size: 6259 DraIII CAC_nnn'GTG Cuts at: 2941 3352 2941 Size: 411 5848 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2540 2960 5266 809 Size: 507 1224 420 2306 1802 Fragments arranged by size: 2306 1802 1224 507 420 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2428 2677 3541 Size: 54 1425 50 275 18 598 249 864 Cuts at: 3541 3706 4595 6224 8 Size: 165 889 1629 43 Fragments arranged by size: 1629 1425 889 864 598 275 249 165 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3706 1537 Size: 275 1894 4090 Fragments arranged by size: 4090 1894 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2202 2322 4189 1353 Size: 849 120 1867 3423 Fragments arranged by size: 3423 1867 849 120 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2270 2747 4846 5220 5366 387 Size: 1064 138 681 477 2099 374 146 1280 Fragments arranged by size: 2099 1280 1064 681 477 374 146 138 EcoICRI GAG'CTC Cuts at: 2230 3096 2230 Size: 866 5393 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2246 2342 2528 2727 2932 2962 3107 Size: 393 614 96 186 199 205 30 145 Cuts at: 3107 3110 3128 3419 3431 3482 4322 4919 5706 Size: 3 18 291 12 51 840 597 787 Cuts at: 5706 5974 1239 Size: 268 1524 Fragments arranged by size: 1524 840 787 614 597 393 291 268 205 199 186 145 96 51 30 18 12 3 EcoO109I rG'GnC_Cy Cuts at: 2359 2398 2953 3077 3166 2359 Size: 39 555 124 89 5452 Fragments arranged by size: 5452 555 124 89 39 EcoRI G'AATT_C Cuts at: 3568 3946 3568 Size: 378 5881 FspI TGC'GCA Cuts at: 2197 4765 2197 Size: 2568 3691 HaeII r_GCGC'y Cuts at: 1678 2029 2507 2662 5406 1678 Size: 351 478 155 2744 2531 Fragments arranged by size: 2744 2531 478 351 155 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2061 2093 3104 3136 4900 Size: 32 135 32 489 32 1011 32 1764 Cuts at: 4900 4932 4974 5006 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1764 1011 489 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2734 3603 3849 669 Size: 988 281 796 869 246 3079 Fragments arranged by size: 3079 988 869 796 281 246 HindIII A'AGCT_T Cuts at: 748 2750 748 Size: 2002 4257 HpaI GTT'AAC Cuts at: 3849 3849 Size: 6259 KpnI G_GTAC'C Cuts at: 3584 3584 Size: 6259 MfeI C'AATT_G Cuts at: 3858 3858 Size: 6259 MluI A'CGCG_T Cuts at: 6135 6135 Size: 6259 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2106 2560 2629 3297 5373 5557 1305 Size: 801 454 69 668 2076 184 2007 Fragments arranged by size: 2076 2007 801 668 454 184 69 MscI TGG'CCA Cuts at: 10 64 1489 1832 2679 3543 10 Size: 54 1425 343 847 864 2726 Fragments arranged by size: 2726 1425 864 847 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2774 2780 3053 4217 4576 4735 518 Size: 648 1608 6 273 1164 359 159 2042 Fragments arranged by size: 2042 1608 1164 648 359 273 159 6 MspA1I CmG'CkG Cuts at: 1606 2176 2299 2339 2799 2903 3103 3675 4352 Size: 570 123 40 460 104 200 572 677 Cuts at: 4352 5500 5745 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 572 570 460 245 200 123 104 40 NcoI C'CATG_G Cuts at: 513 1065 2004 2016 513 Size: 552 939 12 4756 Fragments arranged by size: 4756 939 552 12 NdeI CA'TA_TG Cuts at: 387 387 Size: 6259 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6259 NotI GC'GGCC_GC Cuts at: 3706 3706 Size: 6259 NspI r_CATG'y Cuts at: 1132 1229 2202 3617 5162 1132 Size: 97 973 1415 1545 2229 Fragments arranged by size: 2229 1545 1415 973 97 PasI CC'CwG_GG Cuts at: 1348 2466 3080 1348 Size: 1118 614 4527 Fragments arranged by size: 4527 1118 614 PciI A'CATG_T Cuts at: 5158 5158 Size: 6259 PflMI CCAn_nnn'nTGG Cuts at: 1108 2941 3190 1108 Size: 1833 249 4177 Fragments arranged by size: 4177 1833 249 PfoI T'CCnGG_A Cuts at: 3168 3396 3168 Size: 228 6031 PmeI GTTT'AAAC Cuts at: 3563 3563 Size: 6259 PmlI CAC'GTG Cuts at: 2426 2671 3490 2426 Size: 245 819 5195 Fragments arranged by size: 5195 819 245 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2474 2506 3180 3212 4328 4360 5841 5873 2474 Size: 32 674 32 1116 32 1481 32 2860 Fragments arranged by size: 2860 1481 1116 674 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2398 2953 3166 2398 Size: 555 213 5491 Fragments arranged by size: 5491 555 213 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6259 PsiI TTA'TAA Cuts at: 3829 3829 Size: 6259 PspOMI G'GGCC_C Cuts at: 2358 2358 Size: 6259 PspXI vC'TCGA_Gb Cuts at: 2562 2562 Size: 6259 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2120 2152 2120 Size: 32 6227 PstI C_TGCA'G Cuts at: 830 2304 2502 2721 3339 3611 830 Size: 1474 198 219 618 272 3478 Fragments arranged by size: 3478 1474 618 272 219 198 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6259 PvuII CAG'CTG Cuts at: 2176 2299 2339 3103 2176 Size: 123 40 764 5332 Fragments arranged by size: 5332 764 123 40 SacI G_AGCT'C Cuts at: 2232 3098 2232 Size: 866 5393 SacII CC_GC'GG Cuts at: 2800 2800 Size: 6259 SalI G'TCGA_C Cuts at: 1936 3601 1936 Size: 1665 4594 SbfI CC_TGCA'GG Cuts at: 2502 3611 2502 Size: 1109 5150 ScaI AGT'ACT Cuts at: 4507 6219 4507 Size: 1712 4547 SfcI C'TryA_G Cuts at: 826 1045 2109 2158 2300 2498 2717 3335 3607 Size: 219 1064 49 142 198 219 618 272 Cuts at: 3607 4742 5423 5614 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1135 1064 681 618 272 219 219 198 191 142 49 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6259 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6259 SmaI CCC'GGG Cuts at: 1872 2066 3023 3586 1872 Size: 194 957 563 4545 Fragments arranged by size: 4545 957 563 194 SmlI C'TyrA_G Cuts at: 820 1017 1943 2480 2562 4363 5264 5526 820 Size: 197 926 537 82 1801 901 262 1553 Fragments arranged by size: 1801 1553 926 901 537 262 197 82 SnaBI TAC'GTA Cuts at: 493 4050 493 Size: 3557 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6259 SphI G_CATG'C Cuts at: 1132 2202 3617 1132 Size: 1070 1415 3774 Fragments arranged by size: 3774 1415 1070 SspI AAT'ATT Cuts at: 5 52 4183 5 Size: 47 4131 2081 Fragments arranged by size: 4131 2081 47 StuI AGG'CCT Cuts at: 1517 3227 1517 Size: 1710 4549 StyI C'CwwG_G Cuts at: 513 1065 2004 2016 2093 3051 3698 513 Size: 552 939 12 77 958 647 3074 Fragments arranged by size: 3074 958 939 647 552 77 12 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2544 3197 3335 4286 4303 4456 4641 Size: 1255 964 653 138 951 17 153 185 Cuts at: 4641 5086 325 Size: 445 1498 Fragments arranged by size: 1498 1255 964 951 653 445 185 153 138 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3037 3501 4505 Size: 274 80 33 51 1443 1060 464 1004 Cuts at: 4505 6217 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1060 1004 464 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6219 6251 6219 Size: 32 6227 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6259 XbaI T'CTAG_A Cuts at: 3595 3595 Size: 6259 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2624 3187 3323 2624 Size: 563 136 5560 Fragments arranged by size: 5560 563 136 XhoI C'TCGA_G Cuts at: 1943 2562 1943 Size: 619 5640 XmaI C'CCGG_G Cuts at: 1870 2064 3021 3584 1870 Size: 194 957 563 4545 Fragments arranged by size: 4545 957 563 194 XmnI GAAnn'nnTTC Cuts at: 4388 4388 Size: 6259 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4067 276 Size: 53 83 186 936 2533 2468 Fragments arranged by size: 2533 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI RsrII SanDI SapI SexAI SfiI SfoI SrfI SwaI