Restriction map for
pFN21AB6617
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# NO CUT ENZYMES ( 121 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I AspEI AspI
AviII AvrII BamHI BbeI BbrPI
BcgI BciVI BclI BfaI BfrI
BglII BlnI BsaAI BsaBI BsaI
BseAI BsiEI BsiWI BsmI Bsp1407I
BspDI BspEI BspLU11I BsrGI BssHII
Bst1107I BstBI BstEII BstPI BstXI
BstZ17I ClaI CpoI DraI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HindIII HpaI HphI
KasI KspI MaeI MamI MfeI
MluI MroI MseI MunI NarI
NdeI NheI NotI NruI NsiI
NspI NspV PacI PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TfiI Tru9I Tsp509I Tth111I XbaI
XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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Acc65I 340 GGTACC G^GTACC
AcyI 472 GACGCC G[GA]^CG[CT]C
AlwI 939 GGATC GGATC(4/5)
AocI 1161 CCTCAGG CC^T[ACGT]AGG
Asp718I 340 GGTACC G^GTACC
BbsI 412 GTCTTC GAAGAC(2/6)
BbvCI 405 CCTCAGC CCTCAGC(-5/-2)
BlpI 966 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 966 GCTGAGC GC^T[ACGT]AGC
BpuAI 412 GTCTTC GAAGAC(2/6)
BsaHI 472 GACGCC G[GA]^CG[CT]C
BsaWI 767 ACCGGA [AT]^CCGG[AT]
BsmBI 1072 GAGACG CGTCTC(1/5)
BspHI 824 TCATGA T^CATGA
BsrBI 1209 CCGCTC CCGCTC(-3/-3)
BssSI 1057 CACGAG CACGAG(-5/-1)
BstYI 939 GGATCT [GA]^GATC[CT]
Bsu36I 1161 CCTCAGG CC^T[ACGT]AGG
CelII 966 GCTGAGC GC^T[ACGT]AGC
DraIII 1042 CACGTTGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 102 GAAGAG CTCTTC(1/4)
Eco81I 1161 CCTCAGG CC^T[ACGT]AGG
Hin1I 472 GACGCC G[GA]^CG[CT]C
HincII 577 GTCAAC GT[CT]^[GA]AC
HindII 577 GTCAAC GT[CT]^[GA]AC
KpnI 340 GGTACC GGTAC^C
Ksp632I 102 GAAGAG CTCTTC(1/4)
MaeII 1043 ACGT A^CGT
MaeIII 148 GTCAC ^GT[ACGT]AC
MflI 939 GGATCT [GA]^GATC[CT]
NaeI 1124 GCCGGC GCC^GGC
NcoI 674 CCATGG C^CATGG
NgoMIV 1124 GCCGGC G^CCGGC
PaeR7I 481 CTCGAG C^TCGAG
PvuII 43 CAGCTG CAG^CTG
RcaI 824 TCATGA T^CATGA
SapI 102 GAAGAGC GCTCTTC(1/4)
SmaI 218 CCCGGG CCC^GGG
Tsp45I 148 GTCAC ^GT[GC]AC
XhoI 481 CTCGAG C^TCGAG
XhoII 939 GGATCT [GA]^GATC[CT]
XmaCI 218 CCCGGG C^CCGGG
XmaI 218 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AlwNI 859 CAGGCGCTG CAG[ACGT][ACGT][ACGT]^CTG
937 CAGGATCTG
AspHI 105 GAGCTC G[AT]GC[AT]^C
1078 GAGCTC
AvaI 218 CCCGGG C^[CT]CG[GA]G
481 CTCGAG
BalI 875 TGGCCA TGG^CCA
890 TGGCCA
BsgI 982 CTGCAC GTGCAG(16/14)
856 GTGCAG
BsiHKAI 105 GAGCTC G[AT]GC[AT]^C
1078 GAGCTC
BsmAI 787 GAGAC GTCTC(1/5)
1072 GAGAC
BsoBI 218 CCCGGG C^[CT]CG[GA]G
481 CTCGAG
BsrDI 74 CATTGC GCAATG(2/0)
389 GCAATG
BsrFI 722 ACCGGC [GA]^CCGG[CT]
1124 GCCGGC
BsrI 51 CCAGT ACTGG(1/-1)
624 CCAGT
BstAPI 317 GCAAGACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
858 GCAGGCGCTGC
Cfr10I 722 ACCGGC [GA]^CCGG[CT]
1124 GCCGGC
EcoNI 794 CCTTCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
846 CCTGGGGCAGG
EcoT14I 327 CCTTGG C^C[AT][AT]GG
674 CCATGG
FauI 1026 GCGGG CCCGC(4/6)
1035 GCGGG
HgaI 472 GACGC GACGC(5/10)
410 GCGTC
MluNI 875 TGGCCA TGG^CCA
890 TGGCCA
MscI 875 TGGCCA TGG^CCA
890 TGGCCA
MslI 460 CACAACAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
633 CATGGCCGTG
MspA1I 43 CAGCTG C[AC]G^C[GT]G
1024 CAGCGG
PflMI 878 CCAAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
936 CCAGGATCTGG
PpuMI 779 GGGACCT [GA]G^G[AT]CC[CT]
1183 GGGACCC
SacI 105 GAGCTC GAGCT^C
1078 GAGCTC
SmlI 481 CTCGAG C^T[CT][GA]AG
976 CTCAAG
StyI 327 CCTTGG C^C[AT][AT]GG
674 CCATGG
Van91I 878 CCAAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
936 CCAGGATCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AccII AciI AfaI AluI AvaII
BanI BanII BbvI BcnI BglI
BmyI BpmI BsaJI BseRI BsiYI
BslI BsmFI Bsp1286I BspMI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EcoO109I EcoRII Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII ItaI MboI MboII
MnlI MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PleI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI TaqI TseI TspRI
TthHB8I XcmI