(Circular) (Six-base) MAPSORT of: pFN21AB6617.seq Check: 6905 from: 1 to: 5941 pFN21AB6617 5941 bp With 182 enzymes: SgfI * April 15, 2010 09:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2074 2345 2848 2074 Size: 271 503 5167 Fragments arranged by size: 5167 503 271 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3751 278 Size: 53 83 186 936 2215 2468 Fragments arranged by size: 2468 2215 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3284 1937 Size: 1347 4594 Acc65I G'GTAC_C Cuts at: 2345 3262 2345 Size: 917 5024 AclI AA'CG_TT Cuts at: 1563 4068 4441 1563 Size: 2505 373 3063 Fragments arranged by size: 3063 2505 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2213 2630 4004 5388 5656 1632 Size: 581 417 1374 1384 268 1917 Fragments arranged by size: 1917 1384 1374 581 417 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5744 AflIII A'CryG_T Cuts at: 1203 4840 5817 1203 Size: 3637 977 1327 Fragments arranged by size: 3637 1327 977 AgeI A'CCGG_T Cuts at: 4772 4772 Size: 5941 AhdI GACnn_n'nnGTC Cuts at: 4670 4670 Size: 5941 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5941 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3041 1184 Size: 1857 4084 AlwNI CAG_nnn'CTG Cuts at: 2869 2947 3684 5256 2869 Size: 78 737 1572 3554 Fragments arranged by size: 3554 1572 737 78 ApaLI G'TGCA_C Cuts at: 3998 5154 3998 Size: 1156 4785 ApoI r'AATT_y Cuts at: 1493 3250 3476 3628 5737 1493 Size: 1757 226 152 2109 1697 Fragments arranged by size: 2109 1757 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4495 160 Size: 872 3463 1606 Fragments arranged by size: 3463 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5941 AvaI C'yCGr_G Cuts at: 1870 1943 2223 2486 3266 1870 Size: 73 280 263 780 4545 Fragments arranged by size: 4545 780 280 263 73 BamHI G'GATC_C Cuts at: 3271 3638 3271 Size: 367 5574 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2345 2357 3171 3262 4717 Size: 325 217 627 558 12 814 91 1455 Cuts at: 4717 618 Size: 1842 Fragments arranged by size: 1842 1455 814 627 558 325 217 91 12 BanII G_rGCy'C Cuts at: 1391 2046 2114 2941 3087 3260 1391 Size: 655 68 827 146 173 4072 Fragments arranged by size: 4072 827 655 173 146 68 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2410 928 Size: 971 511 4459 Fragments arranged by size: 4459 971 511 BbvCI CC'TCA_GC Cuts at: 2411 2411 Size: 5941 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4132 4166 1903 Size: 34 2195 34 3678 Fragments arranged by size: 3678 2195 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3833 5043 3833 Size: 1210 4731 BclI T'GATC_A Cuts at: 1548 1548 Size: 5941 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2661 2670 2903 Size: 107 122 71 631 605 989 9 233 Cuts at: 2903 3102 4552 136 Size: 199 1450 1525 Fragments arranged by size: 1525 1450 989 631 605 233 199 122 107 71 9 BglII A'GATC_T Cuts at: 5936 5936 Size: 5941 BlpI GC'TnA_GC Cuts at: 2972 3358 2972 Size: 386 5555 Bme1580I G_kGCm'C Cuts at: 1790 2362 4002 5158 1790 Size: 572 1640 1156 2573 Fragments arranged by size: 2573 1640 1156 572 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5941 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3672 4630 442 Size: 416 53 83 399 24 2255 958 1753 Fragments arranged by size: 2255 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5941 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2128 2128 Size: 5941 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2030 2123 2252 2273 2453 2623 2810 4601 Size: 791 93 129 21 180 170 187 1791 Cuts at: 4601 1239 Size: 2579 Fragments arranged by size: 2579 1791 791 187 180 170 129 93 21 Bpu10I CC'TnA_GC Cuts at: 2384 2411 2384 Size: 27 5914 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2966 4066 4931 5229 2966 Size: 1100 865 298 3678 Fragments arranged by size: 3678 1100 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4604 882 Size: 617 3105 2219 Fragments arranged by size: 3105 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3732 493 Size: 711 2528 2702 Fragments arranged by size: 2702 2528 711 BsaBI GATnn'nnATC Cuts at: 5935 5935 Size: 5941 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2478 3748 4130 4765 Size: 53 83 186 936 945 1270 382 635 Cuts at: 4765 275 Size: 1451 Fragments arranged by size: 1451 1270 945 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2772 4374 4772 5046 5193 5604 2772 Size: 1602 398 274 147 411 3109 Fragments arranged by size: 3109 1602 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2361 2391 1178 Size: 30 165 30 958 30 4728 Fragments arranged by size: 4728 958 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2708 2969 3089 1186 Size: 1522 261 120 4038 Fragments arranged by size: 4038 1522 261 120 BseYI C'CCAG_C Cuts at: 2140 2361 2659 2872 2889 5144 5640 2140 Size: 221 298 213 17 2255 496 2441 Fragments arranged by size: 2441 2255 496 298 221 213 17 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2882 2972 1163 Size: 573 1146 90 4132 Fragments arranged by size: 4132 1146 573 90 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3391 4152 5180 1540 Size: 275 186 1390 761 1028 2301 Fragments arranged by size: 2301 1390 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2114 3087 4002 4087 5158 2114 Size: 973 915 85 1071 2897 Fragments arranged by size: 2897 1071 973 915 85 BsmI GAATG_Cn' Cuts at: 3452 3545 3452 Size: 93 5848 BsmBI CGTCTCn'nnnn_ Cuts at: 3071 3071 Size: 5941 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2046 2114 2362 2941 3087 3260 4002 Size: 399 256 68 248 579 146 173 742 Cuts at: 4002 4087 5158 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 579 399 256 248 173 146 85 68 BspEI T'CCGG_A Cuts at: 5604 5604 Size: 5941 BspHI T'CATG_A Cuts at: 2829 3830 5560 2829 Size: 1001 1730 3210 Fragments arranged by size: 3210 1730 1001 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2074 2336 2345 2848 3296 844 Size: 1230 262 9 503 448 3489 Fragments arranged by size: 3489 1230 503 448 262 9 BsrBI CCG'CTC Cuts at: 1394 1823 3216 3828 1394 Size: 429 1393 612 3507 Fragments arranged by size: 3507 1393 612 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2078 2401 4436 4610 65 Size: 1183 830 323 2035 174 1396 Fragments arranged by size: 2035 1396 1183 830 323 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2727 3129 4585 4772 1700 Size: 110 917 402 1456 187 2869 Fragments arranged by size: 2869 1456 917 402 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5941 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5941 BssSI C'ACGA_G Cuts at: 3062 4001 5013 3062 Size: 939 1012 3990 Fragments arranged by size: 3990 1012 939 BstAPI GCAn_nnn'nTGC Cuts at: 794 2328 2869 794 Size: 1534 541 3866 Fragments arranged by size: 3866 1534 541 BstBI TT'CG_AA Cuts at: 4745 4745 Size: 5941 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5941 BstYI r'GATC_y Cuts at: 1972 2944 3271 3638 4023 4040 5492 5578 5590 Size: 972 327 367 385 17 1452 86 12 Cuts at: 5590 5936 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 972 385 367 346 327 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3167 3167 Size: 5941 BtgI C'CryG_G Cuts at: 513 1065 2004 2643 2679 2779 513 Size: 552 939 639 36 100 3675 Fragments arranged by size: 3675 939 639 552 100 36 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5202 Fragments arranged by size: 5202 683 56 BtsI GCAGTG_nn' Cuts at: 2680 2732 3457 4251 4271 2680 Size: 52 725 794 20 4350 Fragments arranged by size: 4350 794 725 52 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5941 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5906 DraI TTT'AAA Cuts at: 3245 3245 Size: 5941 DraIII CAC_nnn'GTG Cuts at: 3052 3052 Size: 5941 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4948 809 Size: 507 3632 1802 Fragments arranged by size: 3632 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2640 2729 2880 Size: 54 1425 50 275 18 810 89 151 Cuts at: 2880 2895 2949 3388 4277 5906 8 Size: 15 54 439 889 1629 43 Fragments arranged by size: 1629 1425 889 810 439 275 151 89 54 54 50 43 18 15 EagI C'GGCC_G Cuts at: 1537 1812 3388 1537 Size: 275 1576 4090 Fragments arranged by size: 4090 1576 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2102 3871 1353 Size: 749 1769 3423 Fragments arranged by size: 3423 1769 749 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2806 4528 4902 5048 387 Size: 1064 138 1217 1722 374 146 1280 Fragments arranged by size: 1722 1280 1217 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2112 3085 2112 Size: 973 4968 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2030 2123 2213 2252 2273 2453 2623 Size: 393 398 93 90 39 21 180 170 Cuts at: 2623 2630 2810 4004 4601 5388 5656 1239 Size: 7 180 1194 597 787 268 1524 Fragments arranged by size: 1524 1194 787 597 398 393 268 180 180 170 93 90 39 21 7 EcoNI CCTnn'n_nnAGG Cuts at: 2803 2855 2803 Size: 52 5889 EcoO109I rG'GnC_Cy Cuts at: 2428 2449 2785 3139 3189 2428 Size: 21 336 354 50 5180 Fragments arranged by size: 5180 354 336 50 21 EcoRI G'AATT_C Cuts at: 3250 3628 3250 Size: 378 5563 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3250 3250 Size: 5941 FspI TGC'GCA Cuts at: 4447 4447 Size: 5941 HaeII r_GCGC'y Cuts at: 1678 2131 2744 2870 2909 5088 1678 Size: 453 613 126 39 2179 2531 Fragments arranged by size: 2531 2179 613 453 126 39 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2361 2393 4582 4614 4656 Size: 32 135 32 789 32 2189 32 42 Cuts at: 4656 4688 1373 Size: 32 2626 Fragments arranged by size: 2626 2189 789 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2584 3285 3531 669 Size: 988 281 646 701 246 3079 Fragments arranged by size: 3079 988 701 646 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5941 HpaI GTT'AAC Cuts at: 3531 3531 Size: 5941 KpnI G_GTAC'C Cuts at: 2349 3266 2349 Size: 917 5024 MfeI C'AATT_G Cuts at: 3540 3540 Size: 5941 MluI A'CGCG_T Cuts at: 5817 5817 Size: 5941 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2085 5055 5239 1305 Size: 780 2970 184 2007 Fragments arranged by size: 2970 2007 780 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2882 2897 10 Size: 54 1425 343 1050 15 3054 Fragments arranged by size: 3054 1425 1050 343 54 15 MslI CAynn'nnrTG Cuts at: 518 1166 2469 2642 3899 4258 4417 518 Size: 648 1303 173 1257 359 159 2042 Fragments arranged by size: 2042 1303 1257 648 359 173 159 MspA1I CmG'CkG Cuts at: 1606 2050 3031 3357 4034 5182 5427 1606 Size: 444 981 326 677 1148 245 2120 Fragments arranged by size: 2120 1148 981 677 444 326 245 NaeI GCC'GGC Cuts at: 3131 3131 Size: 5941 NcoI C'CATG_G Cuts at: 513 1065 2004 2679 513 Size: 552 939 675 3775 Fragments arranged by size: 3775 939 675 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5941 NgoMIV G'CCGG_C Cuts at: 3129 3129 Size: 5941 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5941 NotI GC'GGCC_GC Cuts at: 3388 3388 Size: 5941 NspI r_CATG'y Cuts at: 1132 1229 3299 4844 1132 Size: 97 2070 1545 2229 Fragments arranged by size: 2229 2070 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5941 PciI A'CATG_T Cuts at: 4840 4840 Size: 5941 PflMI CCAn_nnn'nTGG Cuts at: 1108 2889 2947 1108 Size: 1781 58 4102 Fragments arranged by size: 4102 1781 58 PfoI T'CCnGG_A Cuts at: 2250 2250 Size: 5941 PmeI GTTT'AAAC Cuts at: 3245 3245 Size: 5941 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4010 4042 5523 5555 4010 Size: 32 1481 32 4396 Fragments arranged by size: 4396 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2785 3189 2785 Size: 404 5537 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5941 PsiI TTA'TAA Cuts at: 3511 3511 Size: 5941 PspXI vC'TCGA_Gb Cuts at: 2486 2486 Size: 5941 PstI C_TGCA'G Cuts at: 830 2507 2718 2739 3030 3138 3180 3293 830 Size: 1677 211 21 291 108 42 113 3478 Fragments arranged by size: 3478 1677 291 211 113 108 42 21 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5941 PvuII CAG'CTG Cuts at: 2050 2050 Size: 5941 SacI G_AGCT'C Cuts at: 2114 3087 2114 Size: 973 4968 SalI G'TCGA_C Cuts at: 1936 3283 1936 Size: 1347 4594 SanDI GG'GwC_CC Cuts at: 3189 3189 Size: 5941 SapI GCTCTTCn'nnn_ Cuts at: 2102 2102 Size: 5941 SbfI CC_TGCA'GG Cuts at: 3293 3293 Size: 5941 ScaI AGT'ACT Cuts at: 4189 5901 4189 Size: 1712 4229 SfcI C'TryA_G Cuts at: 826 1045 2503 2714 2735 3026 3134 3176 3289 Size: 219 1458 211 21 291 108 42 113 Cuts at: 3289 4424 5105 5296 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1458 1135 681 291 219 211 191 113 108 42 21 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5941 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5941 SmaI CCC'GGG Cuts at: 1872 2225 3268 1872 Size: 353 1043 4545 Fragments arranged by size: 4545 1043 353 SmlI C'TyrA_G Cuts at: 820 1017 1943 2486 2981 4045 4946 5208 820 Size: 197 926 543 495 1064 901 262 1553 Fragments arranged by size: 1553 1064 926 901 543 495 262 197 SnaBI TAC'GTA Cuts at: 493 3732 493 Size: 3239 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5941 SphI G_CATG'C Cuts at: 1132 3299 1132 Size: 2167 3774 SspI AAT'ATT Cuts at: 5 52 3865 5 Size: 47 3813 2081 Fragments arranged by size: 3813 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5941 StyI C'CwwG_G Cuts at: 513 1065 2004 2332 2679 3380 513 Size: 552 939 328 347 701 3074 Fragments arranged by size: 3074 939 701 552 347 328 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2849 2864 2881 3968 3985 4138 4323 Size: 1255 1269 15 17 1087 17 153 185 Cuts at: 4323 4768 325 Size: 445 1498 Fragments arranged by size: 1498 1269 1255 1087 445 185 153 17 17 15 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4187 5899 96 Size: 274 80 33 51 1443 2210 1712 138 Fragments arranged by size: 2210 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5901 5933 5901 Size: 32 5909 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5941 XbaI T'CTAG_A Cuts at: 3277 3277 Size: 5941 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2777 2890 2905 2777 Size: 113 15 5813 Fragments arranged by size: 5813 113 15 XhoI C'TCGA_G Cuts at: 1943 2486 1943 Size: 543 5398 XmaI C'CCGG_G Cuts at: 1870 2223 3266 1870 Size: 353 1043 4545 Fragments arranged by size: 4545 1043 353 XmnI GAAnn'nnTTC Cuts at: 4070 4070 Size: 5941 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3749 276 Size: 53 83 186 936 2215 2468 Fragments arranged by size: 2468 2215 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BstXI BstZ17I EcoRV FseI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PsrI RsrII SacII SexAI SfiI SfoI SrfI SwaI