Restriction map for
pFN21AB6617
(Linear) (Six-base) MAPSORT of: KIBB6617.seq Check: 6671 from: 1 to: 1236
KIBB6617.orf
With 182 enzymes: SgfI *
October 7, 2009 14:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 69 340 843 1236
Size: 69 271 503 393
Fragments arranged by size:
503 393 271 69
Acc65I G'GTAC_C
Cuts at: 0 340 1236
Size: 340 896
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 208 625 1236
Size: 208 417 611
Fragments arranged by size:
611 417 208
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1036 1236
Size: 1036 200
AlwNI CAG_nnn'CTG
Cuts at: 0 864 942 1236
Size: 864 78 294
Fragments arranged by size:
864 294 78
AvaI C'yCGr_G
Cuts at: 0 218 481 1236
Size: 218 263 755
Fragments arranged by size:
755 263 218
BanI G'GyrC_C
Cuts at: 0 340 352 1166 1236
Size: 340 12 814 70
Fragments arranged by size:
814 340 70 12
BanII G_rGCy'C
Cuts at: 0 41 109 936 1082 1236
Size: 41 68 827 146 154
Fragments arranged by size:
827 154 146 68 41
BbsI GAAGACnn'nnnn_
Cuts at: 0 405 1236
Size: 405 831
BbvCI CC'TCA_GC
Cuts at: 0 406 1236
Size: 406 830
BglI GCCn_nnn'nGGC
Cuts at: 0 656 665 898 1097 1236
Size: 656 9 233 199 139
Fragments arranged by size:
656 233 199 139 9
BlpI GC'TnA_GC
Cuts at: 0 967 1236
Size: 967 269
Bme1580I G_kGCm'C
Cuts at: 0 357 1236
Size: 357 879
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 123 1236
Size: 123 1113
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 25 118 247 268 448 618 805 1236
Size: 25 93 129 21 180 170 187 431
Fragments arranged by size:
431 187 180 170 129 93 25 21
Bpu10I CC'TnA_GC
Cuts at: 0 379 406 1236
Size: 379 27 830
Fragments arranged by size:
830 379 27
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 961 1236
Size: 961 275
BsaHI Gr'CG_yC
Cuts at: 0 473 1236
Size: 473 763
BsaWI w'CCGG_w
Cuts at: 0 767 1236
Size: 767 469
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 356 386 1236
Size: 356 30 850
Fragments arranged by size:
850 356 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 703 964 1084 1236
Size: 703 261 120 152
Fragments arranged by size:
703 261 152 120
BseYI C'CCAG_C
Cuts at: 0 135 356 654 867 884 1236
Size: 135 221 298 213 17 352
Fragments arranged by size:
352 298 221 213 135 17
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 877 967 1236
Size: 877 90 269
Fragments arranged by size:
877 269 90
BsiHKAI G_wGCw'C
Cuts at: 0 109 1082 1236
Size: 109 973 154
Fragments arranged by size:
973 154 109
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1066 1236
Size: 1066 170
Bsp1286I G_dGCh'C
Cuts at: 0 41 109 357 936 1082 1236
Size: 41 68 248 579 146 154
Fragments arranged by size:
579 248 154 146 68 41
BspHI T'CATG_A
Cuts at: 0 824 1236
Size: 824 412
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 69 331 340 843 1236
Size: 69 262 9 503 393
Fragments arranged by size:
503 393 262 69 9
BsrBI CCG'CTC
Cuts at: 0 1211 1236
Size: 1211 25
BsrDI GCAATG_nn'
Cuts at: 0 73 396 1236
Size: 73 323 840
Fragments arranged by size:
840 323 73
BsrFI r'CCGG_y
Cuts at: 0 722 1124 1236
Size: 722 402 112
Fragments arranged by size:
722 402 112
BssSI C'ACGA_G
Cuts at: 0 1057 1236
Size: 1057 179
BstAPI GCAn_nnn'nTGC
Cuts at: 0 323 864 1236
Size: 323 541 372
Fragments arranged by size:
541 372 323
BstYI r'GATC_y
Cuts at: 0 939 1236
Size: 939 297
Bsu36I CC'TnA_GG
Cuts at: 0 1162 1236
Size: 1162 74
BtgI C'CryG_G
Cuts at: 0 638 674 774 1236
Size: 638 36 100 462
Fragments arranged by size:
638 462 100 36
BtsI GCAGTG_nn'
Cuts at: 0 675 727 1236
Size: 675 52 509
Fragments arranged by size:
675 509 52
DraIII CAC_nnn'GTG
Cuts at: 0 1047 1236
Size: 1047 189
EaeI y'GGCC_r
Cuts at: 0 635 724 875 890 944 1236
Size: 635 89 151 15 54 292
Fragments arranged by size:
635 292 151 89 54 15
EarI CTCTTCn'nnn_
Cuts at: 0 97 1236
Size: 97 1139
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 801 1236
Size: 801 435
EcoICRI GAG'CTC
Cuts at: 0 107 1080 1236
Size: 107 973 156
Fragments arranged by size:
973 156 107
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 25 118 208 247 268 448 618 625
Size: 25 93 90 39 21 180 170 7
Cuts at: 625 805 1236
Size: 180 431
Fragments arranged by size:
431 180 180 170 93 90 39 25
21 7
EcoNI CCTnn'n_nnAGG
Cuts at: 0 798 850 1236
Size: 798 52 386
Fragments arranged by size:
798 386 52
EcoO109I rG'GnC_Cy
Cuts at: 0 423 444 780 1134 1184 1236
Size: 423 21 336 354 50 52
Fragments arranged by size:
423 354 336 52 50 21
HaeII r_GCGC'y
Cuts at: 0 126 739 865 904 1236
Size: 126 613 126 39 332
Fragments arranged by size:
613 332 126 126 39
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 356 388 1236
Size: 356 32 848
Fragments arranged by size:
848 356 32
HincII GTy'rAC
Cuts at: 0 579 1236
Size: 579 657
KpnI G_GTAC'C
Cuts at: 0 344 1236
Size: 344 892
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 80 1236
Size: 80 1156
MscI TGG'CCA
Cuts at: 0 877 892 1236
Size: 877 15 344
Fragments arranged by size:
877 344 15
MslI CAynn'nnrTG
Cuts at: 0 464 637 1236
Size: 464 173 599
Fragments arranged by size:
599 464 173
MspA1I CmG'CkG
Cuts at: 0 45 1026 1236
Size: 45 981 210
Fragments arranged by size:
981 210 45
NaeI GCC'GGC
Cuts at: 0 1126 1236
Size: 1126 110
NcoI C'CATG_G
Cuts at: 0 674 1236
Size: 674 562
NgoMIV G'CCGG_C
Cuts at: 0 1124 1236
Size: 1124 112
PflMI CCAn_nnn'nTGG
Cuts at: 0 884 942 1236
Size: 884 58 294
Fragments arranged by size:
884 294 58
PfoI T'CCnGG_A
Cuts at: 0 245 1236
Size: 245 991
PpuMI rG'GwC_Cy
Cuts at: 0 780 1184 1236
Size: 780 404 52
Fragments arranged by size:
780 404 52
PspXI vC'TCGA_Gb
Cuts at: 0 481 1236
Size: 481 755
PstI C_TGCA'G
Cuts at: 0 502 713 734 1025 1133 1175 1236
Size: 502 211 21 291 108 42 61
Fragments arranged by size:
502 291 211 108 61 42 21
PvuII CAG'CTG
Cuts at: 0 45 1236
Size: 45 1191
SacI G_AGCT'C
Cuts at: 0 109 1082 1236
Size: 109 973 154
Fragments arranged by size:
973 154 109
SanDI GG'GwC_CC
Cuts at: 0 1184 1236
Size: 1184 52
SapI GCTCTTCn'nnn_
Cuts at: 0 97 1236
Size: 97 1139
SfcI C'TryA_G
Cuts at: 0 498 709 730 1021 1129 1171 1236
Size: 498 211 21 291 108 42 65
Fragments arranged by size:
498 291 211 108 65 42 21
SmaI CCC'GGG
Cuts at: 0 220 1236
Size: 220 1016
SmlI C'TyrA_G
Cuts at: 0 481 976 1236
Size: 481 495 260
Fragments arranged by size:
495 481 260
StyI C'CwwG_G
Cuts at: 0 327 674 1236
Size: 327 347 562
Fragments arranged by size:
562 347 327
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 844 859 876 1236
Size: 844 15 17 360
Fragments arranged by size:
844 360 17 15
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 772 885 900 1236
Size: 772 113 15 336
Fragments arranged by size:
772 336 113 15
XhoI C'TCGA_G
Cuts at: 0 481 1236
Size: 481 755
XmaI C'CCGG_G
Cuts at: 0 218 1236
Size: 218 1018
Enzymes that do cut:
AarI Acc65I AcuI AlfI AlwNI AvaI BanI BanII
BbsI BbvCI BglI BlpI Bme1580I BplI BpmI Bpu10I
BpuEI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstYI Bsu36I BtgI BtsI DraIII EaeI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII
KpnI MmeI MscI MslI MspA1I NaeI NcoI NgoMIV
PflMI PfoI PpuMI PspXI PstI PvuII SacI SanDI
SapI SfcI SmaI SmlI StyI TaqII XcmI XhoI
XmaI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AflIII AgeI AhdI
AleI AloI ApaI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BcgI BciVI BclI BfrBI
BglII BmgBI BmrI BmtI BsaI BsaAI BsaBI BsiEI
BsiWI BsmI BspEI BsrGI BssHII BstBI BstEII BstXI
BstZ17I BtgZI ClaI CspCI DraI DrdI EagI EcoRI
EcoRV FalI FseI FspI FspAI HindIII HpaI KasI
MfeI MluI NarI NdeI NheI NotI NruI NsiI
NspI PacI PasI PciI PmeI PmlI PpiI PshAI
PsiI PspOMI PsrI PvuI RsrII SacII SalI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI
SphI SrfI SspI StuI SwaI TatI TstI Tth111I
XbaI XmnI ZraI