Restriction map for
pFN21AB0467
##################################
# NO CUT ENZYMES ( 136 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaLI AscI AseI
AsnI Asp718I AspEI AvaI AvrII
BalI BamHI BbeI BbrPI BbvCI
BciVI BclI BcnI BfrI BglII
BlnI BpmI BsaI BsaWI BseAI
BsgI BsiEI BsiWI BsoBI BspDI
BspEI BspHI BspLU11I BsrDI BsrFI
BssHII Bst1107I BstBI BstEII BstPI
BstUI BstZ17I Bsu36I Cfr10I ClaI
CpoI DraI DraIII DrdI DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI HaeII HapII
HincII HindII HpaI HpaII KasI
KpnI KspI MluI MluNI MroI
MscI MspI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PleI PmaCI PmeI
PmlI PshAI PshBI PstI PvuI
RcaI RsrII SacII SalI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI Sse8387I StuI SwaI TaqI
Tsp45I TspRI TthHB8I XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AclI 1322 AACGTT AA^CGTT
AcyI 1316 GACGCC G[GA]^CG[CT]C
AlwNI 745 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 889 GAACATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1453 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1511 TGCGCA TGC^GCA
BanI 19 GGCACC G^G[CT][GA]CC
BcgI 740 CGACACAGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 649 GCCCAAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 302 TACGTG [CT]AC^GT[GA]
BsaBI 1356 GATGCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1316 GACGCC G[GA]^CG[CT]C
BsmI 702 GAATGC GAATGC(1/-1)
BspMI 1423 GCAGGT ACCTGC(4/8)
BsrI 1526 CCAGT ACTGG(1/-1)
BssSI 937 CTCGTG CACGAG(-5/-1)
BstXI 9 CCAGCGGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 70 AGATCC [GA]^GATC[CT]
CfoI 1512 GCGC GCG^C
EcoNI 79 CCTCCTAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 1511 TGCGCA TGC^GCA
HhaI 1512 GCGC GCG^C
Hin1I 1316 GACGCC G[GA]^CG[CT]C
HinP1I 1512 GCGC G^CGC
HindIII 1531 AAGCTT A^AGCTT
MaeIII 782 GTAAC ^GT[ACGT]AC
MamI 1356 GATGCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 349 CAATTG C^AATTG
MflI 70 AGATCC [GA]^GATC[CT]
MunI 349 CAATTG C^AATTG
NspI 1519 GCATGC [GA]CATG^[CT]
PflFI 1453 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 605 CCAAACTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1466 AGGTCCT [GA]G^G[AT]CC[CT]
Psp1406I 1322 AACGTT AA^CGTT
PvuII 745 CAGCTG CAG^CTG
SacI 487 GAGCTC GAGCT^C
SapI 589 GAAGAGC GCTCTTC(1/4)
SphI 1519 GCATGC GCATG^C
Tth111I 1453 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 605 CCAAACTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 108 TCTAGA T^CTAGA
XcmI 47 CCACCCCCAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 70 AGATCC [GA]^GATC[CT]
XmnI 889 GAACATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcsI 1075 GAATTT [GA]^AATT[CT]
1305 GAATTT
AlwI 71 GATCC GGATC(4/5)
177 GGATC
ApaI 42 GGGCCC GGGCC^C
947 GGGCCC
ApoI 1075 GAATTT [GA]^AATT[CT]
1305 GAATTT
AspHI 100 GAGCAC G[AT]GC[AT]^C
487 GAGCTC
BbsI 619 GAAGAC GAAGAC(2/6)
1096 GAAGAC
BlpI 97 GCTGAGC GC^T[ACGT]AGC
567 GCTGAGC
Bpu1102I 97 GCTGAGC GC^T[ACGT]AGC
567 GCTGAGC
BpuAI 619 GAAGAC GAAGAC(2/6)
1096 GAAGAC
BsiHKAI 100 GAGCAC G[AT]GC[AT]^C
487 GAGCTC
BsmBI 911 GAGACG CGTCTC(1/5)
1044 GAGACG
BsmFI 197 GGGAC GGGAC(10/14)
1380 GTCCC
Bsp1407I 2 TGTACA T^GTACA
1183 TGTACA
BsrBI 172 GAGCGG CCGCTC(-3/-3)
535 GAGCGG
BsrGI 2 TGTACA T^GTACA
1183 TGTACA
BstAPI 544 GCACAGATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1514 GCAAGGCATGC
CelII 97 GCTGAGC GC^T[ACGT]AGC
567 GCTGAGC
EcoT14I 90 CCAAGG C^C[AT][AT]GG
632 CCAAGG
FauI 30 GCGGG CCCGC(4/6)
174 GCGGG
HgaI 122 GACGC GACGC(5/10)
1316 GACGC
HinfI 116 GAATC G^A[ACGT]TC
245 GAATC
HphI 60 GGTGA GGTGA(8/7)
713 TCACC
MslI 802 CATGAGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1261 CACATAAATG
SspBI 2 TGTACA T^GTACA
1183 TGTACA
SspI 1031 AATATT AAT^ATT
1086 AATATT
StyI 90 CCAAGG C^C[AT][AT]GG
632 CCAAGG
TfiI 116 GAATC G^A[AT]TC
245 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AciI AfaI AluI AvaII BanII
BbvI BfaI BmyI BsaJI BseRI
BsiYI BslI BsmAI Bsp1286I BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII DraII EarI EcoO109I EcoRII
Fnu4HI FokI HaeIII ItaI Ksp632I
MaeI MaeII MboI MboII MnlI
MseI MspA1I MvaI MwoI NdeII
NlaIII NlaIV RsaI Sau3AI Sau96I
ScrFI SfaNI Tru9I TseI Tsp509I