(Circular) (Six-base) MAPSORT of: pFN21AB0467.seq Check: 4144 from: 1 to: 6247 pFN21AB0467 6247 bp With 182 enzymes: SgfI * April 15, 2010 09:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3419 3419 Size: 6247 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4057 278 Size: 53 83 186 936 2521 2468 Fragments arranged by size: 2521 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3590 1937 Size: 1653 4594 Acc65I G'GTAC_C Cuts at: 3568 3568 Size: 6247 AclI AA'CG_TT Cuts at: 1563 3328 4374 4747 1563 Size: 1765 1046 373 3063 Fragments arranged by size: 3063 1765 1046 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2777 3341 4310 5694 5962 1632 Size: 1145 564 969 1384 268 1917 Fragments arranged by size: 1917 1384 1145 969 564 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6050 AflIII A'CryG_T Cuts at: 1203 5146 6123 1203 Size: 3943 977 1327 Fragments arranged by size: 3943 1327 977 AgeI A'CCGG_T Cuts at: 3544 5078 3544 Size: 1534 4713 AhdI GACnn_n'nnGTC Cuts at: 4976 4976 Size: 6247 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6247 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6247 AlwNI CAG_nnn'CTG Cuts at: 2755 3990 5562 2755 Size: 1235 1572 3440 Fragments arranged by size: 3440 1572 1235 ApaI G_GGCC'C Cuts at: 2051 2956 2051 Size: 905 5342 ApaLI G'TGCA_C Cuts at: 4304 5460 4304 Size: 1156 5091 ApoI r'AATT_y Cuts at: 1493 3080 3310 3556 3782 3934 6043 1493 Size: 1587 230 246 226 152 2109 1697 Fragments arranged by size: 2109 1697 1587 246 230 226 152 AseI AT'TA_AT Cuts at: 160 1032 4801 160 Size: 872 3769 1606 Fragments arranged by size: 3769 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6247 AvaI C'yCGr_G Cuts at: 1870 1943 3572 1870 Size: 73 1629 4545 Fragments arranged by size: 4545 1629 73 BamHI G'GATC_C Cuts at: 3577 3944 3577 Size: 367 5880 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2024 3568 5023 618 Size: 325 217 627 237 1544 1455 1842 Fragments arranged by size: 1842 1544 1455 627 325 237 217 BanII G_rGCy'C Cuts at: 1391 2051 2496 2956 3566 1391 Size: 660 445 460 610 4072 Fragments arranged by size: 4072 660 610 460 445 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2631 3108 928 Size: 971 732 477 4067 Fragments arranged by size: 4067 971 732 477 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2734 2768 4438 4472 1903 Size: 34 797 34 1670 34 3678 Fragments arranged by size: 3678 1670 797 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4139 5349 4139 Size: 1210 5037 BclI T'GATC_A Cuts at: 1548 1548 Size: 6247 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2660 4858 136 Size: 107 122 71 631 605 988 2198 1525 Fragments arranged by size: 2198 1525 988 631 605 122 107 71 BglII A'GATC_T Cuts at: 6242 6242 Size: 6247 BlpI GC'TnA_GC Cuts at: 2103 2573 3664 2103 Size: 470 1091 4686 Fragments arranged by size: 4686 1091 470 Bme1580I G_kGCm'C Cuts at: 1790 2051 2956 4308 5464 1790 Size: 261 905 1352 1156 2573 Fragments arranged by size: 2573 1352 1156 905 261 BmgBI CAC'GTC Cuts at: 1318 3053 1318 Size: 1735 4512 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3525 3978 4936 Size: 416 53 83 399 24 2108 453 958 Cuts at: 4936 442 Size: 1753 Fragments arranged by size: 2108 1753 958 453 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6247 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4907 1239 Size: 3668 2579 Bpu10I CC'TnA_GC Cuts at: 3069 3069 Size: 6247 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4372 5237 5535 4372 Size: 865 298 5084 Fragments arranged by size: 5084 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4910 882 Size: 617 3411 2219 Fragments arranged by size: 3411 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2309 4038 493 Size: 711 1105 1729 2702 Fragments arranged by size: 2702 1729 1105 711 BsaBI GATnn'nnATC Cuts at: 3365 6241 3365 Size: 2876 3371 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3322 4054 4436 5071 Size: 53 83 186 936 1789 732 382 635 Cuts at: 5071 275 Size: 1451 Fragments arranged by size: 1789 1451 936 732 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3544 4680 5078 5352 5499 5910 3544 Size: 1136 398 274 147 411 3881 Fragments arranged by size: 3881 1136 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3461 3491 1178 Size: 30 165 30 2058 30 3934 Fragments arranged by size: 3934 2058 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2390 2504 2552 2582 2680 2780 1186 Size: 1204 114 48 30 98 100 4653 Fragments arranged by size: 4653 1204 114 100 98 48 30 BseYI C'CCAG_C Cuts at: 5450 5946 5450 Size: 496 5751 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5674 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3697 4458 5486 1540 Size: 275 186 1696 761 1028 2301 Fragments arranged by size: 2301 1696 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2109 2496 4308 4393 5464 2109 Size: 387 1812 85 1071 2892 Fragments arranged by size: 2892 1812 1071 387 85 BsmI GAATG_Cn' Cuts at: 2713 3758 3851 2713 Size: 1045 93 5109 Fragments arranged by size: 5109 1045 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2910 3043 2910 Size: 133 6114 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2051 2109 2496 2956 3566 4308 4393 Size: 399 261 58 387 460 610 742 85 Cuts at: 4393 5464 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 610 460 399 387 261 85 58 BspEI T'CCGG_A Cuts at: 5910 5910 Size: 6247 BspHI T'CATG_A Cuts at: 4136 5866 4136 Size: 1730 4517 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3419 3602 844 Size: 2575 183 3489 Fragments arranged by size: 3489 2575 183 BsrBI CCG'CTC Cuts at: 1394 1823 2179 2542 4134 1394 Size: 429 356 363 1592 3507 Fragments arranged by size: 3507 1592 429 363 356 BsrDI GCAATG_nn' Cuts at: 65 1248 4742 4916 65 Size: 1183 3494 174 1396 Fragments arranged by size: 3494 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3544 4891 5078 1700 Size: 110 1734 1347 187 2869 Fragments arranged by size: 2869 1734 1347 187 110 BsrGI T'GTAC_A Cuts at: 96 2007 3188 96 Size: 1911 1181 3155 Fragments arranged by size: 3155 1911 1181 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6247 BssSI C'ACGA_G Cuts at: 2942 4307 5319 2942 Size: 1365 1012 3870 Fragments arranged by size: 3870 1365 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2555 3525 794 Size: 1761 970 3516 Fragments arranged by size: 3516 1761 970 BstBI TT'CG_AA Cuts at: 5051 5051 Size: 6247 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6247 BstXI CCAn_nnnn'nTGG Cuts at: 2021 2021 Size: 6247 BstYI r'GATC_y Cuts at: 1972 2075 3577 3944 4329 4346 5798 5884 5896 Size: 103 1502 367 385 17 1452 86 12 Cuts at: 5896 6242 1972 Size: 346 1977 Fragments arranged by size: 1977 1502 1452 385 367 346 103 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5695 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5508 Fragments arranged by size: 5508 683 56 BtsI GCAGTG_nn' Cuts at: 3763 4557 4577 3763 Size: 794 20 5433 Fragments arranged by size: 5433 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6247 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6212 DraI TTT'AAA Cuts at: 3551 3551 Size: 6247 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5254 809 Size: 507 3938 1802 Fragments arranged by size: 3938 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3694 4583 6212 Size: 54 1425 50 275 18 1864 889 1629 Cuts at: 6212 8 Size: 43 Fragments arranged by size: 1864 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3694 1537 Size: 275 1882 4090 Fragments arranged by size: 4090 1882 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2589 2757 2967 3294 3399 4177 1353 Size: 1236 168 210 327 105 778 3423 Fragments arranged by size: 3423 1236 778 327 210 168 105 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4834 5208 5354 387 Size: 1064 138 3245 374 146 1280 Fragments arranged by size: 3245 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2494 2494 Size: 6247 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2777 3341 4310 4907 5694 5962 1239 Size: 393 1145 564 969 597 787 268 1524 Fragments arranged by size: 1524 1145 969 787 597 564 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2088 2088 Size: 6247 EcoO109I rG'GnC_Cy Cuts at: 2047 2048 2952 2953 3472 2047 Size: 1 904 1 519 4822 Fragments arranged by size: 4822 904 519 1 1 EcoRI G'AATT_C Cuts at: 3556 3934 3556 Size: 378 5869 FspI TGC'GCA Cuts at: 3518 4753 3518 Size: 1235 5012 HaeII r_GCGC'y Cuts at: 1678 5394 1678 Size: 3716 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2235 2267 3461 3493 4888 Size: 32 135 32 663 32 1194 32 1395 Cuts at: 4888 4920 4962 4994 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1395 1194 663 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3591 3837 669 Size: 988 281 1653 246 3079 Fragments arranged by size: 3079 1653 988 281 246 HindIII A'AGCT_T Cuts at: 748 3536 748 Size: 2788 3459 HpaI GTT'AAC Cuts at: 3837 3837 Size: 6247 KpnI G_GTAC'C Cuts at: 3572 3572 Size: 6247 MfeI C'AATT_G Cuts at: 2354 3846 2354 Size: 1492 4755 MluI A'CGCG_T Cuts at: 6123 6123 Size: 6247 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2149 5361 5545 1305 Size: 844 3212 184 2007 Fragments arranged by size: 3212 2007 844 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4425 Fragments arranged by size: 4425 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2811 3270 4205 4564 4723 518 Size: 648 1645 459 935 359 159 2042 Fragments arranged by size: 2042 1645 935 648 459 359 159 MspA1I CmG'CkG Cuts at: 1606 2017 2035 2752 3663 4340 5488 5733 1606 Size: 411 18 717 911 677 1148 245 2120 Fragments arranged by size: 2120 1148 911 717 677 411 245 18 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5695 NdeI CA'TA_TG Cuts at: 387 387 Size: 6247 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6247 NotI GC'GGCC_GC Cuts at: 3694 3694 Size: 6247 NspI r_CATG'y Cuts at: 1132 1229 3528 3605 5150 1132 Size: 97 2299 77 1545 2229 Fragments arranged by size: 2299 2229 1545 97 77 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6247 PciI A'CATG_T Cuts at: 5146 5146 Size: 6247 PflMI CCAn_nnn'nTGG Cuts at: 1108 2616 1108 Size: 1508 4739 PfoI T'CCnGG_A Cuts at: 3465 3465 Size: 6247 PmeI GTTT'AAAC Cuts at: 3551 3551 Size: 6247 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2588 2620 4316 4348 5829 5861 2588 Size: 32 1696 32 1481 32 2974 Fragments arranged by size: 2974 1696 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3472 3472 Size: 6247 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6247 PsiI TTA'TAA Cuts at: 3817 3817 Size: 6247 PspOMI G'GGCC_C Cuts at: 2047 2952 2047 Size: 905 5342 PstI C_TGCA'G Cuts at: 830 3599 830 Size: 2769 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6247 PvuII CAG'CTG Cuts at: 2752 2752 Size: 6247 SacI G_AGCT'C Cuts at: 2496 2496 Size: 6247 SalI G'TCGA_C Cuts at: 1936 3589 1936 Size: 1653 4594 SapI GCTCTTCn'nnn_ Cuts at: 2589 2589 Size: 6247 SbfI CC_TGCA'GG Cuts at: 3599 3599 Size: 6247 ScaI AGT'ACT Cuts at: 4495 6207 4495 Size: 1712 4535 SfcI C'TryA_G Cuts at: 826 1045 3595 4730 5411 5602 826 Size: 219 2550 1135 681 191 1471 Fragments arranged by size: 2550 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6247 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6247 SmaI CCC'GGG Cuts at: 1872 3574 1872 Size: 1702 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4351 5252 5514 820 Size: 197 926 2408 901 262 1553 Fragments arranged by size: 2408 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4038 493 Size: 3545 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6247 SphI G_CATG'C Cuts at: 1132 3528 3605 1132 Size: 2396 77 3774 Fragments arranged by size: 3774 2396 77 SspI AAT'ATT Cuts at: 5 52 3038 3093 4171 5 Size: 47 2986 55 1078 2081 Fragments arranged by size: 2986 2081 1078 55 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6247 StyI C'CwwG_G Cuts at: 513 1065 2095 2637 3686 513 Size: 552 1030 542 1049 3074 Fragments arranged by size: 3074 1049 1030 552 542 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2643 3497 4274 4291 4444 4629 5074 Size: 1255 1063 854 777 17 153 185 445 Cuts at: 5074 325 Size: 1498 Fragments arranged by size: 1498 1255 1063 854 777 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2007 3188 3342 Size: 274 80 33 51 1443 30 1181 154 Cuts at: 3342 4493 6205 96 Size: 1151 1712 138 Fragments arranged by size: 1712 1443 1181 1151 274 154 138 80 51 33 30 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6207 6239 6207 Size: 32 6215 Tth111I GACn'n_nGTC Cuts at: 1316 3461 1316 Size: 2145 4102 XbaI T'CTAG_A Cuts at: 2113 3583 2113 Size: 1470 4777 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2059 2059 Size: 6247 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6247 XmaI C'CCGG_G Cuts at: 1870 3572 1870 Size: 1702 4545 XmnI GAAnn'nnTTC Cuts at: 2898 4376 2898 Size: 1478 4769 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4055 276 Size: 53 83 186 936 2521 2468 Fragments arranged by size: 2521 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI