Restriction map for
pFN21AB0467
(Linear) (Six-base) MAPSORT of: KIBB0467.seq Check: 2618 from: 1 to: 1542
KIBB0467.orf
With 182 enzymes: SgfI *
October 7, 2009 14:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1414 1542
Size: 1414 128
AclI AA'CG_TT
Cuts at: 0 1323 1542
Size: 1323 219
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 772 1336 1542
Size: 772 564 206
Fragments arranged by size:
772 564 206
AlwNI CAG_nnn'CTG
Cuts at: 0 750 1542
Size: 750 792
ApaI G_GGCC'C
Cuts at: 0 46 951 1542
Size: 46 905 591
Fragments arranged by size:
905 591 46
ApoI r'AATT_y
Cuts at: 0 1075 1305 1542
Size: 1075 230 237
Fragments arranged by size:
1075 237 230
BanI G'GyrC_C
Cuts at: 0 19 1542
Size: 19 1523
BanII G_rGCy'C
Cuts at: 0 46 491 951 1542
Size: 46 445 460 591
Fragments arranged by size:
591 460 445 46
BbsI GAAGACnn'nnnn_
Cuts at: 0 626 1103 1542
Size: 626 477 439
Fragments arranged by size:
626 477 439
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 729 763 1542
Size: 729 34 779
Fragments arranged by size:
779 729 34
BglI GCCn_nnn'nGGC
Cuts at: 0 655 1542
Size: 655 887
BlpI GC'TnA_GC
Cuts at: 0 98 568 1542
Size: 98 470 974
Fragments arranged by size:
974 470 98
Bme1580I G_kGCm'C
Cuts at: 0 46 951 1542
Size: 46 905 591
Fragments arranged by size:
905 591 46
BmgBI CAC'GTC
Cuts at: 0 1048 1542
Size: 1048 494
BmrI ACTGGGnnnn_n'
Cuts at: 0 1520 1542
Size: 1520 22
Bpu10I CC'TnA_GC
Cuts at: 0 1064 1542
Size: 1064 478
BsaAI yAC'GTr
Cuts at: 0 304 1542
Size: 304 1238
BsaBI GATnn'nnATC
Cuts at: 0 1360 1542
Size: 1360 182
BsaHI Gr'CG_yC
Cuts at: 0 1317 1542
Size: 1317 225
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1456 1486 1542
Size: 1456 30 56
Fragments arranged by size:
1456 56 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 385 499 547 577 675 775 1542
Size: 385 114 48 30 98 100 767
Fragments arranged by size:
767 385 114 100 98 48 30
BsiHKAI G_wGCw'C
Cuts at: 0 104 491 1542
Size: 104 387 1051
Fragments arranged by size:
1051 387 104
BsmI GAATG_Cn'
Cuts at: 0 708 1542
Size: 708 834
BsmBI CGTCTCn'nnnn_
Cuts at: 0 905 1038 1542
Size: 905 133 504
Fragments arranged by size:
905 504 133
Bsp1286I G_dGCh'C
Cuts at: 0 46 104 491 951 1542
Size: 46 58 387 460 591
Fragments arranged by size:
591 460 387 58 46
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1414 1542
Size: 1414 128
BsrBI CCG'CTC
Cuts at: 0 174 537 1542
Size: 174 363 1005
Fragments arranged by size:
1005 363 174
BsrGI T'GTAC_A
Cuts at: 0 2 1183 1542
Size: 2 1181 359
Fragments arranged by size:
1181 359 2
BssSI C'ACGA_G
Cuts at: 0 937 1542
Size: 937 605
BstAPI GCAn_nnn'nTGC
Cuts at: 0 550 1520 1542
Size: 550 970 22
Fragments arranged by size:
970 550 22
BstXI CCAn_nnnn'nTGG
Cuts at: 0 16 1542
Size: 16 1526
BstYI r'GATC_y
Cuts at: 0 70 1542
Size: 70 1472
EarI CTCTTCn'nnn_
Cuts at: 0 584 752 962 1289 1394 1542
Size: 584 168 210 327 105 148
Fragments arranged by size:
584 327 210 168 148 105
EcoICRI GAG'CTC
Cuts at: 0 489 1542
Size: 489 1053
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 772 1336 1542
Size: 772 564 206
Fragments arranged by size:
772 564 206
EcoNI CCTnn'n_nnAGG
Cuts at: 0 83 1542
Size: 83 1459
EcoO109I rG'GnC_Cy
Cuts at: 0 42 43 947 948 1467 1542
Size: 42 1 904 1 519 75
Fragments arranged by size:
904 519 75 42 1 1
FspI TGC'GCA
Cuts at: 0 1513 1542
Size: 1513 29
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 230 262 1456 1488 1542
Size: 230 32 1194 32 54
Fragments arranged by size:
1194 230 54 32 32
HindIII A'AGCT_T
Cuts at: 0 1531 1542
Size: 1531 11
MfeI C'AATT_G
Cuts at: 0 349 1542
Size: 349 1193
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 144 1542
Size: 144 1398
MslI CAynn'nnrTG
Cuts at: 0 806 1265 1542
Size: 806 459 277
Fragments arranged by size:
806 459 277
MspA1I CmG'CkG
Cuts at: 0 12 30 747 1542
Size: 12 18 717 795
Fragments arranged by size:
795 717 18 12
NspI r_CATG'y
Cuts at: 0 1523 1542
Size: 1523 19
PflMI CCAn_nnn'nTGG
Cuts at: 0 611 1542
Size: 611 931
PfoI T'CCnGG_A
Cuts at: 0 1460 1542
Size: 1460 82
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 583 615 1542
Size: 583 32 927
Fragments arranged by size:
927 583 32
PpuMI rG'GwC_Cy
Cuts at: 0 1467 1542
Size: 1467 75
PspOMI G'GGCC_C
Cuts at: 0 42 947 1542
Size: 42 905 595
Fragments arranged by size:
905 595 42
PvuII CAG'CTG
Cuts at: 0 747 1542
Size: 747 795
SacI G_AGCT'C
Cuts at: 0 491 1542
Size: 491 1051
SapI GCTCTTCn'nnn_
Cuts at: 0 584 1542
Size: 584 958
SphI G_CATG'C
Cuts at: 0 1523 1542
Size: 1523 19
SspI AAT'ATT
Cuts at: 0 1033 1088 1542
Size: 1033 55 454
Fragments arranged by size:
1033 454 55
StyI C'CwwG_G
Cuts at: 0 90 632 1542
Size: 90 542 910
Fragments arranged by size:
910 542 90
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 638 1492 1542
Size: 638 854 50
Fragments arranged by size:
854 638 50
TatI w'GTAC_w
Cuts at: 0 2 1183 1337 1542
Size: 2 1181 154 205
Fragments arranged by size:
1181 205 154 2
Tth111I GACn'n_nGTC
Cuts at: 0 1456 1542
Size: 1456 86
XbaI T'CTAG_A
Cuts at: 0 108 1542
Size: 108 1434
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 54 1542
Size: 54 1488
XmnI GAAnn'nnTTC
Cuts at: 0 893 1542
Size: 893 649
Enzymes that do cut:
AarI AclI AcuI AlwNI ApaI ApoI BanI BanII
BbsI BcgI BglI BlpI Bme1580I BmgBI BmrI Bpu10I
BsaAI BsaBI BsaHI BsaXI BseRI BsiHKAI BsmI BsmBI
Bsp1286I BspMI BsrBI BsrGI BssSI BstAPI BstXI BstYI
EarI EcoICRI Eco57MI EcoNI EcoO109I FspI Hin4I HindIII
MfeI MmeI MslI MspA1I NspI PflMI PfoI PpiI
PpuMI PspOMI PvuII SacI SapI SphI SspI StyI
TaqII TatI Tth111I XbaI XcmI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BbeI BbvCI BciVI BclI BfrBI
BglII BmtI BplI BpmI BpuEI BsaI BsaWI BseYI
BsgI BsiEI BsiWI BspEI BspHI BsrDI BsrFI BssHII
BstBI BstEII BstZ17I Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EciI EcoRI
EcoRV FalI FseI FspAI HaeII HincII HpaI KasI
KpnI MluI MscI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PciI PmeI
PmlI PshAI PsiI PspXI PsrI PstI PvuI RsrII
SacII SalI SanDI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI
StuI SwaI TstI XhoI XmaI ZraI