Restriction map for
pFN21AB0462
(Circular) (Six-base) MAPSORT of: pFN21AB0462.seq Check: 3194 from: 1 to: 7633
pFN21AB0462 7633 bp
With 182 enzymes: SgfI *
April 15, 2010 09:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3684 3793 4459 3684
Size: 109 666 6858
Fragments arranged by size:
6858 666 109
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2034 5443 278
Size: 53 83 186 936 498 3409 2468
Fragments arranged by size:
3409 2468 936 498 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4976 1937
Size: 3039 4594
Acc65I G'GTAC_C
Cuts at: 4954 4954
Size: 7633
AclI AA'CG_TT
Cuts at: 1563 5760 6133 1563
Size: 4197 373 3063
Fragments arranged by size:
4197 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3347 3552 3942 4124 4667 5696 7080 7348
Size: 1715 205 390 182 543 1029 1384 268
Cuts at: 7348 1632
Size: 1917
Fragments arranged by size:
1917 1715 1384 1029 543 390 268 205
182
AfeI AGC'GCT
Cuts at: 2024 2136 2024
Size: 112 7521
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7436
AflIII A'CryG_T
Cuts at: 1203 4614 6532 7509 1203
Size: 3411 1918 977 1327
Fragments arranged by size:
3411 1918 1327 977
AgeI A'CCGG_T
Cuts at: 2987 6464 2987
Size: 3477 4156
AhdI GACnn_n'nnGTC
Cuts at: 6362 6362
Size: 7633
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7633
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2944 4723 1184
Size: 1760 1779 4094
Fragments arranged by size:
4094 1779 1760
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2744 2776 2744
Size: 32 7601
AlwNI CAG_nnn'CTG
Cuts at: 2265 2605 2830 3172 4342 5376 6948 2265
Size: 340 225 342 1170 1034 1572 2950
Fragments arranged by size:
2950 1572 1170 1034 342 340 225
ApaI G_GGCC'C
Cuts at: 2373 2373
Size: 7633
ApaLI G'TGCA_C
Cuts at: 2174 5690 6846 2174
Size: 3516 1156 2961
Fragments arranged by size:
3516 2961 1156
ApoI r'AATT_y
Cuts at: 1493 3390 4780 4942 5168 5320 7429 1493
Size: 1897 1390 162 226 152 2109 1697
Fragments arranged by size:
2109 1897 1697 1390 226 162 152
AseI AT'TA_AT
Cuts at: 160 1032 6187 160
Size: 872 5155 1606
Fragments arranged by size:
5155 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7633
AvaI C'yCGr_G
Cuts at: 1870 1943 3746 4019 4442 4653 4915 4958 1870
Size: 73 1803 273 423 211 262 43 4545
Fragments arranged by size:
4545 1803 423 273 262 211 73 43
BamHI G'GATC_C
Cuts at: 2383 3333 4963 5330 2383
Size: 950 1630 367 4686
Fragments arranged by size:
4686 1630 950 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2548 2815 2990 3672 3865
Size: 325 217 627 761 267 175 682 193
Cuts at: 3865 4723 4954 6409 618
Size: 858 231 1455 1842
Fragments arranged by size:
1842 1455 858 761 682 627 325 267
231 217 193 175
BanII G_rGCy'C
Cuts at: 1391 2373 2910 2979 3858 4050 4952 1391
Size: 982 537 69 879 192 902 4072
Fragments arranged by size:
4072 982 902 879 537 192 69
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3540 4797 928
Size: 971 1641 1257 3764
Fragments arranged by size:
3764 1641 1257 971
BbvCI CC'TCA_GC
Cuts at: 3572 3572
Size: 7633
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5824 5858 1903
Size: 34 3887 34 3678
Fragments arranged by size:
3887 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3365 5525 6735 3365
Size: 2160 1210 4263
Fragments arranged by size:
4263 2160 1210
BclI T'GATC_A
Cuts at: 1548 4278 1548
Size: 2730 4903
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2159 2660 2799
Size: 107 122 71 631 605 487 501 139
Cuts at: 2799 3031 3040 6244 136
Size: 232 9 3204 1525
Fragments arranged by size:
3204 1525 631 605 501 487 232 139
122 107 71 9
BglII A'GATC_T
Cuts at: 4854 7628 4854
Size: 2774 4859
BlpI GC'TnA_GC
Cuts at: 2131 2280 5050 2131
Size: 149 2770 4714
Fragments arranged by size:
4714 2770 149
Bme1580I G_kGCm'C
Cuts at: 1790 2178 2204 2373 2995 3228 3675 5694 6850
Size: 388 26 169 622 233 447 2019 1156
Cuts at: 6850 1790
Size: 2573
Fragments arranged by size:
2573 2019 1156 622 447 388 233 169
26
BmgBI CAC'GTC
Cuts at: 1318 3781 1318
Size: 2463 5170
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3039 5364 6322
Size: 416 53 83 399 24 1622 2325 958
Cuts at: 6322 442
Size: 1753
Fragments arranged by size:
2325 1753 1622 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7633
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3475 3475
Size: 7633
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3359 3452 4001 4364 4655 6293 1239
Size: 2120 93 549 363 291 1638 2579
Fragments arranged by size:
2579 2120 1638 549 363 291 93
Bpu10I CC'TnA_GC
Cuts at: 2573 3572 2573
Size: 999 6634
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2684 2732 2894 5758 6623 6921 2684
Size: 48 162 2864 865 298 3396
Fragments arranged by size:
3396 2864 865 298 162 48
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3475 4774 6296 882
Size: 617 1976 1299 1522 2219
Fragments arranged by size:
2219 1976 1522 1299 617
BsaAI yAC'GTr
Cuts at: 493 1204 4617 5424 493
Size: 711 3413 807 2702
Fragments arranged by size:
3413 2702 807 711
BsaBI GATnn'nnATC
Cuts at: 4154 7627 4154
Size: 3473 4160
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2031 3117 4431 4563
Size: 53 83 186 936 498 1086 1314 132
Cuts at: 4563 5440 5822 6457 275
Size: 877 382 635 1451
Fragments arranged by size:
1451 1314 1086 936 877 635 498 382
186 132 83 53
BsaWI w'CCGG_w
Cuts at: 2386 2987 3231 3969 4602 6066 6464 6738 6885
Size: 601 244 738 633 1464 398 274 147
Cuts at: 6885 7296 2386
Size: 411 2723
Fragments arranged by size:
2723 1464 738 633 601 411 398 274
244 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2231 2261 2744 2774 3887
Size: 30 165 30 828 30 483 30 1113
Cuts at: 3887 3917 4256 4286 1178
Size: 30 339 30 4525
Fragments arranged by size:
4525 1113 828 483 339 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2513 2839 2842 3356 3764 3911 4780 4789
Size: 1327 326 3 514 408 147 869 9
Cuts at: 4789 1186
Size: 4030
Fragments arranged by size:
4030 1327 869 514 408 326 147 9
3
BseYI C'CCAG_C
Cuts at: 2014 2301 2942 3497 3562 6836 7332 2014
Size: 287 641 555 65 3274 496 2315
Fragments arranged by size:
3274 2315 641 555 496 287 65
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2432 2618 3185 3289 4928 1163
Size: 573 696 186 567 104 1639 3868
Fragments arranged by size:
3868 1639 696 573 567 186 104
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2059 4523 5083 5844 6872 1540
Size: 275 186 58 2464 560 761 1028 2301
Fragments arranged by size:
2464 2301 1028 761 560 275 186 58
BsiHKAI G_wGCw'C
Cuts at: 2132 2178 2334 2353 2400 2910 5694 5779 6850
Size: 46 156 19 47 510 2784 85 1071
Cuts at: 6850 2132
Size: 2915
Fragments arranged by size:
2915 2784 1071 510 156 85 47 46
19
BsiWI C'GTAC_G
Cuts at: 4617 4617
Size: 7633
BsmI GAATG_Cn'
Cuts at: 2233 3075 3508 4743 5144 5237 2233
Size: 842 433 1235 401 93 4629
Fragments arranged by size:
4629 1235 842 433 401 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2317 4792 2317
Size: 2475 5158
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2132 2178 2204 2334 2353 2373 2400
Size: 399 342 46 26 130 19 20 27
Cuts at: 2400 2910 2979 2995 3228 3675 3858 4050 4952
Size: 510 69 16 233 447 183 192 902
Cuts at: 4952 5694 5779 6850 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 902 742 510 447 399 342
233 192 183 130 85 69 46 27
26 20 19 16
BspEI T'CCGG_A
Cuts at: 2386 3969 4602 7296 2386
Size: 1583 633 2694 2723
Fragments arranged by size:
2723 2694 1583 633
BspHI T'CATG_A
Cuts at: 5522 7252 5522
Size: 1730 5903
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2838 3684 3793 4168 4459 4988 844
Size: 1994 846 109 375 291 529 3489
Fragments arranged by size:
3489 1994 846 529 375 291 109
BsrBI CCG'CTC
Cuts at: 1394 1823 5520 1394
Size: 429 3697 3507
Fragments arranged by size:
3697 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4717 4874 6128 6302 65
Size: 1183 3469 157 1254 174 1396
Fragments arranged by size:
3469 1396 1254 1183 174 157
BsrFI r'CCGG_y
Cuts at: 1700 1810 2987 4518 4522 4629 4895 6277 6464
Size: 110 1177 1531 4 107 266 1382 187
Cuts at: 6464 1700
Size: 2869
Fragments arranged by size:
2869 1531 1382 1177 266 187 110 107
4
BsrGI T'GTAC_A
Cuts at: 96 2871 4878 96
Size: 2775 2007 2851
Fragments arranged by size:
2851 2775 2007
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7633
BssSI C'ACGA_G
Cuts at: 2105 4862 5693 6705 2105
Size: 2757 831 1012 3033
Fragments arranged by size:
3033 2757 1012 831
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2605 794
Size: 1811 5822
BstBI TT'CG_AA
Cuts at: 6437 6437
Size: 7633
BstEII G'GTnAC_C
Cuts at: 1184 3616 1184
Size: 2432 5201
BstXI CCAn_nnnn'nTGG
Cuts at: 2950 3430 2950
Size: 480 7153
BstYI r'GATC_y
Cuts at: 1972 2383 3168 3285 3333 3598 4641 4854 4963
Size: 411 785 117 48 265 1043 213 109
Cuts at: 4963 5330 5715 5732 7184 7270 7282 7628 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1043 785 411 385 367 346
265 213 117 109 86 48 17 12
Bsu36I CC'TnA_GG
Cuts at: 2258 2258
Size: 7633
BtgI C'CryG_G
Cuts at: 513 1065 2004 2375 2856 3759 3772 4566 513
Size: 552 939 371 481 903 13 794 3580
Fragments arranged by size:
3580 939 903 794 552 481 371 13
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4141 487
Size: 683 56 2915 3979
Fragments arranged by size:
3979 2915 683 56
BtsI GCAGTG_nn'
Cuts at: 2552 5149 5943 5963 2552
Size: 2597 794 20 4222
Fragments arranged by size:
4222 2597 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7633
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3469 3504 529
Size: 35 2905 35 4658
Fragments arranged by size:
4658 2905 35 35
DraI TTT'AAA
Cuts at: 4937 4937
Size: 7633
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3215 6640 809
Size: 507 1899 3425 1802
Fragments arranged by size:
3425 1899 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2592 2661 2800
Size: 54 1425 50 275 18 762 69 139
Cuts at: 2800 2859 4520 4845 4871 5080 5969 7598 8
Size: 59 1661 325 26 209 889 1629 43
Fragments arranged by size:
1661 1629 1425 889 762 325 275 209
139 69 59 54 50 43 26 18
EagI C'GGCC_G
Cuts at: 1537 1812 4520 5080 1537
Size: 275 2708 560 4090
Fragments arranged by size:
4090 2708 560 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3086 3821 4100 4487 4798 4816 5563 1353
Size: 1733 735 279 387 311 18 747 3423
Fragments arranged by size:
3423 1733 747 735 387 311 279 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6220 6594 6740 387
Size: 1064 138 4631 374 146 1280
Fragments arranged by size:
4631 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2908 2908
Size: 7633
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3347 3359 3452 3552 3942 4001 4124
Size: 393 1715 12 93 100 390 59 123
Cuts at: 4124 4364 4655 4667 5696 6293 7080 7348 1239
Size: 240 291 12 1029 597 787 268 1524
Fragments arranged by size:
1715 1524 1029 787 597 393 390 291
268 240 123 100 93 59 12 12
EcoNI CCTnn'n_nnAGG
Cuts at: 2563 2626 4398 2563
Size: 63 1772 5798
Fragments arranged by size:
5798 1772 63
EcoO109I rG'GnC_Cy
Cuts at: 2369 2370 2527 2653 2737 2806 2827 4081 4186
Size: 1 157 126 84 69 21 1254 105
Cuts at: 4186 4240 4504 4919 2369
Size: 54 264 415 5083
Fragments arranged by size:
5083 1254 415 264 157 126 105 84
69 54 21 1
EcoRI G'AATT_C
Cuts at: 4942 5320 4942
Size: 378 7255
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3307 4498 3307
Size: 1191 6442
FspI TGC'GCA
Cuts at: 6139 6139
Size: 7633
HaeII r_GCGC'y
Cuts at: 1678 2026 2138 2190 2885 3301 3349 6780 1678
Size: 348 112 52 695 416 48 3431 2531
Fragments arranged by size:
3431 2531 695 416 348 112 52 48
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2231 2263 2316 2348 4647
Size: 32 135 32 659 32 53 32 2299
Cuts at: 4647 4679 6274 6306 6348 6380 1373
Size: 32 1595 32 42 32 2626
Fragments arranged by size:
2626 2299 1595 659 135 53 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4977 5223 669
Size: 988 281 3039 246 3079
Fragments arranged by size:
3079 3039 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7633
HpaI GTT'AAC
Cuts at: 5223 5223
Size: 7633
KpnI G_GTAC'C
Cuts at: 4958 4958
Size: 7633
MfeI C'AATT_G
Cuts at: 5232 5232
Size: 7633
MluI A'CGCG_T
Cuts at: 7509 7509
Size: 7633
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3030 4714 6747 6931 1305
Size: 1725 1684 2033 184 2007
Fragments arranged by size:
2033 2007 1725 1684 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2663 2802 2861 4873 10
Size: 54 1425 343 831 139 59 2012 2770
Fragments arranged by size:
2770 2012 1425 831 343 139 59 54
MslI CAynn'nnrTG
Cuts at: 518 1166 5591 5950 6109 518
Size: 648 4425 359 159 2042
Fragments arranged by size:
4425 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2061 2416 5049 5726 6874 7119 1606
Size: 455 355 2633 677 1148 245 2120
Fragments arranged by size:
2633 2120 1148 677 455 355 245
NaeI GCC'GGC
Cuts at: 4897 4897
Size: 7633
NcoI C'CATG_G
Cuts at: 513 1065 2004 2856 513
Size: 552 939 852 5290
Fragments arranged by size:
5290 939 852 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7633
NgoMIV G'CCGG_C
Cuts at: 4895 4895
Size: 7633
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7633
NotI GC'GGCC_GC
Cuts at: 5080 5080
Size: 7633
NspI r_CATG'y
Cuts at: 1132 1229 3529 4664 4991 6536 1132
Size: 97 2300 1135 327 1545 2229
Fragments arranged by size:
2300 2229 1545 1135 327 97
PasI CC'CwG_GG
Cuts at: 1348 2530 3199 4507 1348
Size: 1182 669 1308 4474
Fragments arranged by size:
4474 1308 1182 669
PciI A'CATG_T
Cuts at: 6532 6532
Size: 7633
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4342 4633 1108
Size: 3234 291 4108
Fragments arranged by size:
4108 3234 291
PfoI T'CCnGG_A
Cuts at: 2667 3153 3276 3336 4182 4548 2667
Size: 486 123 60 846 366 5752
Fragments arranged by size:
5752 846 486 366 123 60
PmeI GTTT'AAAC
Cuts at: 4937 4937
Size: 7633
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2744 2776 5702 5734 7215 7247 2744
Size: 32 2926 32 1481 32 3130
Fragments arranged by size:
3130 2926 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2827 4081 4186 4240 4919 2827
Size: 1254 105 54 679 5541
Fragments arranged by size:
5541 1254 679 105 54
PshAI GACnn'nnGTC
Cuts at: 1613 3723 1613
Size: 2110 5523
PsiI TTA'TAA
Cuts at: 4539 5203 4539
Size: 664 6969
PspOMI G'GGCC_C
Cuts at: 2369 2369
Size: 7633
PstI C_TGCA'G
Cuts at: 830 3466 4985 830
Size: 2636 1519 3478
Fragments arranged by size:
3478 2636 1519
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7633
SacI G_AGCT'C
Cuts at: 2910 2910
Size: 7633
SalI G'TCGA_C
Cuts at: 1936 4975 1936
Size: 3039 4594
SanDI GG'GwC_CC
Cuts at: 4081 4081
Size: 7633
SapI GCTCTTCn'nnn_
Cuts at: 3821 3821
Size: 7633
SbfI CC_TGCA'GG
Cuts at: 4985 4985
Size: 7633
ScaI AGT'ACT
Cuts at: 5881 7593 5881
Size: 1712 5921
SfcI C'TryA_G
Cuts at: 826 1045 2243 3462 4981 6116 6797 6988 826
Size: 219 1198 1219 1519 1135 681 191 1471
Fragments arranged by size:
1519 1471 1219 1198 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2660 2799 3040 2660
Size: 139 241 7253
Fragments arranged by size:
7253 241 139
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7633
SgrAI Cr'CCGG_yG
Cuts at: 1700 2987 1700
Size: 1287 6346
SmaI CCC'GGG
Cuts at: 1872 4917 4960 1872
Size: 3045 43 4545
Fragments arranged by size:
4545 3045 43
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2699 2711 2909 5737 6638 6900
Size: 197 926 756 12 198 2828 901 262
Cuts at: 6900 820
Size: 1553
Fragments arranged by size:
2828 1553 926 901 756 262 198 197
12
SnaBI TAC'GTA
Cuts at: 493 5424 493
Size: 4931 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7633
SphI G_CATG'C
Cuts at: 1132 4991 1132
Size: 3859 3774
SspI AAT'ATT
Cuts at: 5 52 5557 5
Size: 47 5505 2081
Fragments arranged by size:
5505 2081 47
StuI AGG'CCT
Cuts at: 1517 2616 4393 1517
Size: 1099 1777 4757
Fragments arranged by size:
4757 1777 1099
StyI C'CwwG_G
Cuts at: 513 1065 2004 2649 2772 2811 2856 2979 3834
Size: 552 939 645 123 39 45 123 855
Cuts at: 3834 4215 4399 4719 5072 513
Size: 381 184 320 353 3074
Fragments arranged by size:
3074 939 855 645 552 381 353 320
184 123 123 45 39
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2436 3498 5660 5677 5830 6015 6460
Size: 1255 856 1062 2162 17 153 185 445
Cuts at: 6460 325
Size: 1498
Fragments arranged by size:
2162 1498 1255 1062 856 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2871 4878 5879
Size: 274 80 33 51 1443 894 2007 1001
Cuts at: 5879 7591 96
Size: 1712 138
Fragments arranged by size:
2007 1712 1443 1001 894 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7593 7625 7593
Size: 32 7601
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7633
XbaI T'CTAG_A
Cuts at: 4969 4969
Size: 7633
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2656 2011
Size: 645 6988
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7633
XmaI C'CCGG_G
Cuts at: 1870 4915 4958 1870
Size: 3045 43 4545
Fragments arranged by size:
4545 3045 43
XmnI GAAnn'nnTTC
Cuts at: 5762 5762
Size: 7633
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2032 5441 276
Size: 53 83 186 936 498 3409 2468
Fragments arranged by size:
3409 2468 936 498 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI
SalI SanDI SapI SbfI ScaI SfcI SfiI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BbeI BfrBI BstZ17I DraIII EcoRV
FseI FspAI KasI NarI NruI NsiI PacI PmlI
PspXI PsrI PvuII RsrII SacII SexAI SfoI SrfI
SwaI