Restriction map for
pFN21AB6571
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# NO CUT ENZYMES ( 167 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI Asp700I Asp718I AspEI AspI
AvaI AviII AvrII BalI BamHI
BanI BanII BbeI BbrPI BbvCI
BcgI BciVI BfaI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BsaHI BsaWI BseAI BsgI BsiEI
BsiWI BsmBI BsmFI BsmI BsoBI
Bsp1407I BspDI BspEI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstBI BstEII BstPI BstUI
BstXI Bsu36I CelII CfoI Cfr10I
ClaI CpoI DraIII DrdI DsaI
EaeI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT14I FauI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HincII HindII HpaI HphI
KasI KpnI Ksp632I KspI MaeI
MaeII MfeI MluI MluNI MroI
MscI MunI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NlaIV
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI Psp1406I PstI PvuI
PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI Tsp45I
Tth111I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AciI 1110 CCGC CCGC(-3/-1)
AseI 1042 ATTAAT AT^TAAT
AsnI 1042 ATTAAT AT^TAAT
BbsI 624 GAAGAC GAAGAC(2/6)
BbvI 302 GCAGC GCAGC(8/12)
BclI 273 TGATCA T^GATCA
BcnI 261 CCGGG CC^[GC]GG
BpmI 929 CTGGAG CTGGAG(16/14)
BpuAI 624 GAAGAC GAAGAC(2/6)
BsaBI 171 GATACAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 480 GAGACC GGTCTC(1/5)
BseRI 251 CTCCTC GAGGAG(10/8)
BspHI 702 TCATGA T^CATGA
Bst1107I 555 GTATAC GTA^TAC
BstAPI 3 GCAAAAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 555 GTATAC GTA^TAC
Cac8I 1006 GCAGGC GC[ACGT]^[ACGT]GC
DraI 418 TTTAAA TTT^AAA
DraII 1008 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoO109I 1008 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoT22I 119 ATGCAT ATGCA^T
FbaI 273 TGATCA T^GATCA
Fnu4HI 302 GCAGC GC^[ACGT]GC
HapII 261 CCGG C^CGG
HpaII 261 CCGG C^CGG
ItaI 302 GCAGC GC^[ACGT]GC
MamI 171 GATACAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I 1110 CCGCTG C[AC]G^C[GT]G
MspI 261 CCGG C^CGG
NciI 261 CCGGG CC^[GC]GG
NsiI 119 ATGCAT ATGCA^T
NspI 121 GCATGT [GA]CATG^[CT]
PflMI 592 CCAGGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 1042 ATTAAT AT^TAAT
RcaI 702 TCATGA T^CATGA
SfaNI 839 GATGC GCATC(5/9)
SfcI 365 CTACAG C^T[GA][CT]AG
TaqI 488 TCGA T^CGA
TseI 302 GCAGC G^C[AT]GC
TthHB8I 488 TCGA T^CGA
Van91I 592 CCAGGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 13 enzymes) #
###################################
AccI 555 GTATAC GT^[AC][GT]AC
1105 GTAGAC
AlwI 788 GATCC GGATC(4/5)
63 GGATC
AlwNI 368 CAGAGTCTG CAG[ACGT][ACGT][ACGT]^CTG
593 CAGGCACTG
AspHI 820 GAGCAC G[AT]GC[AT]^C
1119 GTGCTC
AvaII 55 GGTCC G^G[AT]CC
727 GGACC
BmyI 820 GAGCAC G[AGT]GC[ACT]^C
1119 GTGCTC
BsaJI 591 CCCAGG C^C[ACGT][ACGT]GG
723 CCCAGG
BsiHKAI 820 GAGCAC G[AT]GC[AT]^C
1119 GTGCTC
Bsp1286I 820 GAGCAC G[AGT]GC[ACT]^C
1119 GTGCTC
BstYI 63 GGATCT [GA]^GATC[CT]
787 AGATCC
HindIII 683 AAGCTT A^AGCTT
872 AAGCTT
MflI 63 GGATCT [GA]^GATC[CT]
787 AGATCC
XhoII 63 GGATCT [GA]^GATC[CT]
787 AGATCC
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# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AcsI AfaI AluI ApoI BsiYI
BslI BsmAI BsrI BssKI BstF5I
BstNI Cfr13I DdeI DpnII EcoRII
FokI HaeIII HinfI MaeIII MboI
MboII MnlI MseI MslI MvaI
MwoI NdeII NlaIII PleI RsaI
Sau3AI Sau96I ScrFI TfiI Tru9I
Tsp509I TspRI