(Linear) (Six-base) MAPSORT of: KIBB6571.seq  Check: 6532  from: 1  to: 1125



KIBB6571.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:21  ..

AccI GT'mk_AC

Cuts at:      0     556    1106    1125 
   Size:        556     550      19

  Fragments arranged by size: 

                 556     550      19
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1029    1125 
   Size:       1029      96
 
AlwNI CAG_nnn'CTG

Cuts at:      0     373     598    1125 
   Size:        373     225     527

  Fragments arranged by size: 

                 527     373     225
 
ApoI r'AATT_y

Cuts at:      0     421     940    1088    1125 
   Size:        421     519     148      37

  Fragments arranged by size: 

                 519     421     148      37
 
AseI AT'TA_AT

Cuts at:      0    1043    1125 
   Size:       1043      82
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     631    1125 
   Size:        631     494
 
BclI T'GATC_A

Cuts at:      0     273    1125 
   Size:        273     852
 
BfrBI ATG'CAT

Cuts at:      0     121    1125 
   Size:        121    1004
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     950    1125 
   Size:        950     175
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     474    1125 
   Size:        474     651
 
BsaBI GATnn'nnATC

Cuts at:      0     175    1125 
   Size:        175     950
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     242    1125 
   Size:        242     883
 
BsiHKAI G_wGCw'C

Cuts at:      0     824    1123    1125 
   Size:        824     299       2

  Fragments arranged by size: 

                 824     299       2
 
Bsp1286I G_dGCh'C

Cuts at:      0     824    1123    1125 
   Size:        824     299       2

  Fragments arranged by size: 

                 824     299       2
 
BspHI T'CATG_A

Cuts at:      0     702    1125 
   Size:        702     423
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0       9    1125 
   Size:          9    1116
 
BstYI r'GATC_y

Cuts at:      0      63     787    1125 
   Size:         63     724     338

  Fragments arranged by size: 

                 724     338      63
 
BstZ17I GTA'TAC

Cuts at:      0     557    1125 
   Size:        557     568
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     320    1111    1125 
   Size:        320     791      14

  Fragments arranged by size: 

                 791     320      14
 
DraI TTT'AAA

Cuts at:      0     420    1125 
   Size:        420     705
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     950    1125 
   Size:        950     175
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1009    1125 
   Size:       1009     116
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     808     840     882     914    1125 
   Size:        808      32      42      32     211

  Fragments arranged by size: 

                 808     211      42      32      32
 
HindIII A'AGCT_T

Cuts at:      0     683     872    1125 
   Size:        683     189     253

  Fragments arranged by size: 

                 683     253     189
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     686    1125 
   Size:        686     439
 
MslI CAynn'nnrTG

Cuts at:      0     358     619    1030    1125 
   Size:        358     261     411      95

  Fragments arranged by size: 

                 411     358     261      95
 
MspA1I CmG'CkG

Cuts at:      0    1112    1125 
   Size:       1112      13
 
NsiI A_TGCA'T

Cuts at:      0     123    1125 
   Size:        123    1002
 
NspI r_CATG'y

Cuts at:      0     125    1125 
   Size:        125    1000
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     598    1125 
   Size:        598     527
 
PfoI T'CCnGG_A

Cuts at:      0     260    1125 
   Size:        260     865
 
PsiI TTA'TAA

Cuts at:      0     229    1125 
   Size:        229     896
 
SfcI C'TryA_G

Cuts at:      0     365    1125 
   Size:        365     760
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1106    1125 
   Size:       1106      19
 
TatI w'GTAC_w

Cuts at:      0     124    1018    1125 
   Size:        124     894     107

  Fragments arranged by size: 

                 894     124     107
 

 Enzymes that do cut:

     AccI     AlfI    AlwNI     ApoI     AseI     BbsI     BclI    BfrBI
     BpmI     BsaI    BsaBI    BseRI  BsiHKAI Bsp1286I    BspHI   BstAPI
    BstYI  BstZ17I    BtgZI     DraI  Eco57MI EcoO109I    Hin4I  HindIII
     MmeI     MslI   MspA1I     NsiI     NspI    PflMI     PfoI     PsiI
     SfcI    TaqII     TatI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AloI     ApaI    ApaLI     AscI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI    BbvCI
     BcgI    BciVI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BplI   Bpu10I    BpuEI    BsaAI    BsaHI    BsaWI    BsaXI
    BseYI     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspMI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI    BstBI   BstEII
    BstXI   Bsu36I     BtgI     BtsI     ClaI    CspCI   DraIII     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII   HincII     HpaI     KasI
     KpnI     MfeI     MluI     MscI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     PacI     PasI     PciI     PmeI
     PmlI     PpiI    PpuMI    PshAI   PspOMI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI
     SwaI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI