(Circular) (Six-base) MAPSORT of: pFN21AB0455.seq Check: 8447 from: 1 to: 6220 pFN21AB0455 6220 bp With 182 enzymes: SgfI * April 15, 2010 09:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3389 3389 Size: 6220 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4030 278 Size: 53 83 186 936 2494 2468 Fragments arranged by size: 2494 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3563 1937 Size: 1626 4594 Acc65I G'GTAC_C Cuts at: 3541 3541 Size: 6220 AclI AA'CG_TT Cuts at: 1563 4347 4720 1563 Size: 2784 373 3063 Fragments arranged by size: 3063 2784 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2102 2264 2288 2837 2996 3060 4283 5667 Size: 470 162 24 549 159 64 1223 1384 Cuts at: 5667 5935 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 1223 549 470 268 162 159 64 24 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6023 AflIII A'CryG_T Cuts at: 1203 3021 3255 5119 6096 1203 Size: 1818 234 1864 977 1327 Fragments arranged by size: 1864 1818 1327 977 234 AgeI A'CCGG_T Cuts at: 5051 5051 Size: 6220 AhdI GACnn_n'nnGTC Cuts at: 3259 4949 3259 Size: 1690 4530 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6220 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2436 1184 Size: 1252 4968 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2360 2392 2818 2850 2360 Size: 32 426 32 5730 Fragments arranged by size: 5730 426 32 32 AlwNI CAG_nnn'CTG Cuts at: 2242 2266 2605 2650 3963 5535 2242 Size: 24 339 45 1313 1572 2927 Fragments arranged by size: 2927 1572 1313 339 45 24 ApaI G_GGCC'C Cuts at: 2695 2695 Size: 6220 ApaLI G'TGCA_C Cuts at: 4277 5433 4277 Size: 1156 5064 ApoI r'AATT_y Cuts at: 1493 3067 3529 3755 3907 6016 1493 Size: 1574 462 226 152 2109 1697 Fragments arranged by size: 2109 1697 1574 462 226 152 AseI AT'TA_AT Cuts at: 160 1032 4774 160 Size: 872 3742 1606 Fragments arranged by size: 3742 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6220 AvaI C'yCGr_G Cuts at: 1870 1943 2346 2744 3329 3389 3434 3545 1870 Size: 73 403 398 585 60 45 111 4545 Fragments arranged by size: 4545 585 403 398 111 73 60 45 BamHI G'GATC_C Cuts at: 3550 3917 3550 Size: 367 5853 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2477 3304 3541 4996 618 Size: 325 217 627 690 827 237 1455 1842 Fragments arranged by size: 1842 1455 827 690 627 325 237 217 BanII G_rGCy'C Cuts at: 1391 2078 2695 2925 3539 1391 Size: 687 617 230 614 4072 Fragments arranged by size: 4072 687 617 614 230 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2125 2184 2984 3229 928 Size: 971 226 59 800 245 3919 Fragments arranged by size: 3919 971 800 245 226 59 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4411 4445 1903 Size: 34 2474 34 3678 Fragments arranged by size: 3678 2474 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4112 5322 4112 Size: 1210 5010 BclI T'GATC_A Cuts at: 1548 2523 1548 Size: 975 5245 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4831 136 Size: 107 122 71 631 605 3159 1525 Fragments arranged by size: 3159 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6215 6215 Size: 6220 BlpI GC'TnA_GC Cuts at: 3637 3637 Size: 6220 Bme1580I G_kGCm'C Cuts at: 1790 2331 2695 4281 5437 1790 Size: 541 364 1586 1156 2573 Fragments arranged by size: 2573 1586 1156 541 364 BmgBI CAC'GTC Cuts at: 1318 3256 1318 Size: 1938 4282 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2325 3951 4909 Size: 416 53 83 399 24 908 1626 958 Cuts at: 4909 442 Size: 1753 Fragments arranged by size: 1753 1626 958 908 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6220 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2298 2582 2820 3368 3494 4880 1239 Size: 1059 284 238 548 126 1386 2579 Fragments arranged by size: 2579 1386 1059 548 284 238 126 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4345 5210 5508 4345 Size: 865 298 5057 Fragments arranged by size: 5057 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4883 882 Size: 617 3384 2219 Fragments arranged by size: 3384 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4011 493 Size: 711 2807 2702 Fragments arranged by size: 2807 2702 711 BsaBI GATnn'nnATC Cuts at: 6214 6214 Size: 6220 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2106 3405 4027 4409 Size: 53 83 186 936 573 1299 622 382 Cuts at: 4409 5044 275 Size: 635 1451 Fragments arranged by size: 1451 1299 936 635 622 573 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4653 5051 5325 5472 5883 4653 Size: 398 274 147 411 4990 Fragments arranged by size: 4990 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2555 2585 2818 2848 1178 Size: 30 165 30 1152 30 233 30 4550 Fragments arranged by size: 4550 1152 233 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2139 3452 1186 Size: 953 1313 3954 Fragments arranged by size: 3954 1313 953 BseYI C'CCAG_C Cuts at: 5423 5919 5423 Size: 496 5724 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2630 1163 Size: 573 894 4753 Fragments arranged by size: 4753 894 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3670 4431 5459 1540 Size: 275 186 1669 761 1028 2301 Fragments arranged by size: 2301 1669 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4281 4366 5437 4281 Size: 85 1071 5064 Fragments arranged by size: 5064 1071 85 BsmI GAATG_Cn' Cuts at: 3731 3824 3731 Size: 93 6127 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2078 2331 2695 2925 3539 4281 4366 Size: 399 288 253 364 230 614 742 85 Cuts at: 4366 5437 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 614 399 364 288 253 230 85 BspEI T'CCGG_A Cuts at: 5883 5883 Size: 6220 BspHI T'CATG_A Cuts at: 4109 5839 4109 Size: 1730 4490 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2241 3093 3389 3575 844 Size: 1397 852 296 186 3489 Fragments arranged by size: 3489 1397 852 296 186 BsrBI CCG'CTC Cuts at: 1394 1823 2887 3442 3499 4107 1394 Size: 429 1064 555 57 608 3507 Fragments arranged by size: 3507 1064 608 555 429 57 BsrDI GCAATG_nn' Cuts at: 65 1248 4715 4889 65 Size: 1183 3467 174 1396 Fragments arranged by size: 3467 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2438 3429 4864 5051 1700 Size: 110 628 991 1435 187 2869 Fragments arranged by size: 2869 1435 991 628 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6220 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6220 BssSI C'ACGA_G Cuts at: 2882 4280 5292 2882 Size: 1398 1012 3810 Fragments arranged by size: 3810 1398 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3469 794 Size: 2675 3545 BstBI TT'CG_AA Cuts at: 5024 5024 Size: 6220 BstEII G'GTnAC_C Cuts at: 1184 2911 1184 Size: 1727 4493 BstXI CCAn_nnnn'nTGG Cuts at: 2011 3103 2011 Size: 1092 5128 BstYI r'GATC_y Cuts at: 1972 2538 2709 3550 3917 4302 4319 5771 5857 Size: 566 171 841 367 385 17 1452 86 Cuts at: 5857 5869 6215 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 841 566 385 367 346 171 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3400 513 Size: 552 939 1396 3333 Fragments arranged by size: 3333 1396 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3394 487 Size: 683 56 2168 3313 Fragments arranged by size: 3313 2168 683 56 BtsI GCAGTG_nn' Cuts at: 2114 2913 3736 4530 4550 2114 Size: 799 823 794 20 3784 Fragments arranged by size: 3784 823 799 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6220 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6185 DraI TTT'AAA Cuts at: 3524 3524 Size: 6220 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5227 809 Size: 507 3911 1802 Fragments arranged by size: 3911 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2292 2802 2949 Size: 54 1425 50 275 18 462 510 147 Cuts at: 2949 3093 3489 3667 4556 6185 8 Size: 144 396 178 889 1629 43 Fragments arranged by size: 1629 1425 889 510 462 396 275 178 147 144 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3667 1537 Size: 275 1855 4090 Fragments arranged by size: 4090 1855 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2308 4150 1353 Size: 955 1842 3423 Fragments arranged by size: 3423 1842 955 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4807 5181 5327 387 Size: 1064 138 3218 374 146 1280 Fragments arranged by size: 3218 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2102 2264 2288 2298 2582 2820 2837 Size: 393 470 162 24 10 284 238 17 Cuts at: 2837 2996 3060 3368 3494 4283 4880 5667 5935 Size: 159 64 308 126 789 597 787 268 Cuts at: 5935 1239 Size: 1524 Fragments arranged by size: 1524 789 787 597 470 393 308 284 268 238 162 159 126 64 24 17 10 EcoNI CCTnn'n_nnAGG Cuts at: 2852 2852 Size: 6220 EcoO109I rG'GnC_Cy Cuts at: 2230 2263 2602 2230 Size: 33 339 5848 Fragments arranged by size: 5848 339 33 EcoRI G'AATT_C Cuts at: 3067 3529 3907 3067 Size: 462 378 5380 Fragments arranged by size: 5380 462 378 FspI TGC'GCA Cuts at: 4726 4726 Size: 6220 HaeII r_GCGC'y Cuts at: 1678 2094 5367 1678 Size: 416 3273 2531 Fragments arranged by size: 3273 2531 416 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2857 2889 2988 3020 3245 Size: 32 135 32 1285 32 99 32 225 Cuts at: 3245 3277 4861 4893 4935 4967 1373 Size: 32 1584 32 42 32 2626 Fragments arranged by size: 2626 1584 1285 225 135 99 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3564 3810 669 Size: 988 281 1626 246 3079 Fragments arranged by size: 3079 1626 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6220 HpaI GTT'AAC Cuts at: 3810 3810 Size: 6220 KpnI G_GTAC'C Cuts at: 3545 3545 Size: 6220 MfeI C'AATT_G Cuts at: 3819 3819 Size: 6220 MluI A'CGCG_T Cuts at: 6096 6096 Size: 6220 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2375 2920 5334 5518 1305 Size: 1070 545 2414 184 2007 Fragments arranged by size: 2414 2007 1070 545 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2294 2804 2951 10 Size: 54 1425 343 462 510 147 3279 Fragments arranged by size: 3279 1425 510 462 343 147 54 MslI CAynn'nnrTG Cuts at: 518 1166 2172 2216 2798 3104 4178 4537 4696 Size: 648 1006 44 582 306 1074 359 159 Cuts at: 4696 518 Size: 2042 Fragments arranged by size: 2042 1074 1006 648 582 359 306 159 44 MspA1I CmG'CkG Cuts at: 1606 2544 3402 3636 4313 5461 5706 1606 Size: 938 858 234 677 1148 245 2120 Fragments arranged by size: 2120 1148 938 858 677 245 234 NaeI GCC'GGC Cuts at: 2440 3431 2440 Size: 991 5229 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4729 Fragments arranged by size: 4729 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6220 NgoMIV G'CCGG_C Cuts at: 2438 3429 2438 Size: 991 5229 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6220 NotI GC'GGCC_GC Cuts at: 3667 3667 Size: 6220 NspI r_CATG'y Cuts at: 1132 1229 3025 3367 3578 5123 1132 Size: 97 1796 342 211 1545 2229 Fragments arranged by size: 2229 1796 1545 342 211 97 PasI CC'CwG_GG Cuts at: 1348 2512 2688 3314 1348 Size: 1164 176 626 4254 Fragments arranged by size: 4254 1164 626 176 PciI A'CATG_T Cuts at: 3021 5119 3021 Size: 2098 4122 PflMI CCAn_nnn'nTGG Cuts at: 1108 2605 3204 1108 Size: 1497 599 4124 Fragments arranged by size: 4124 1497 599 PmeI GTTT'AAAC Cuts at: 3524 3524 Size: 6220 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2818 2850 4289 4321 5802 5834 2818 Size: 32 1439 32 1481 32 3204 Fragments arranged by size: 3204 1481 1439 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2230 2263 2230 Size: 33 6187 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6220 PsiI TTA'TAA Cuts at: 3790 3790 Size: 6220 PspOMI G'GGCC_C Cuts at: 2691 2691 Size: 6220 PstI C_TGCA'G Cuts at: 830 2238 2880 3465 3516 3572 830 Size: 1408 642 585 51 56 3478 Fragments arranged by size: 3478 1408 642 585 56 51 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6220 SacII CC_GC'GG Cuts at: 3403 3403 Size: 6220 SalI G'TCGA_C Cuts at: 1936 3562 1936 Size: 1626 4594 SbfI CC_TGCA'GG Cuts at: 3572 3572 Size: 6220 ScaI AGT'ACT Cuts at: 4468 6180 4468 Size: 1712 4508 SfcI C'TryA_G Cuts at: 826 1045 2234 2258 2876 3461 3512 3568 4703 Size: 219 1189 24 618 585 51 56 1135 Cuts at: 4703 5384 5575 826 Size: 681 191 1471 Fragments arranged by size: 1471 1189 1135 681 618 585 219 191 56 51 24 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6220 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6220 SmaI CCC'GGG Cuts at: 1872 3547 1872 Size: 1675 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4324 5225 5487 820 Size: 197 926 2381 901 262 1553 Fragments arranged by size: 2381 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4011 493 Size: 3518 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6220 SphI G_CATG'C Cuts at: 1132 3367 3578 1132 Size: 2235 211 3774 Fragments arranged by size: 3774 2235 211 SspI AAT'ATT Cuts at: 5 52 4144 5 Size: 47 4092 2081 Fragments arranged by size: 4092 2081 47 StuI AGG'CCT Cuts at: 1517 3116 1517 Size: 1599 4621 StyI C'CwwG_G Cuts at: 513 1065 2004 2916 3008 3659 513 Size: 552 939 912 92 651 3074 Fragments arranged by size: 3074 939 912 651 552 92 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2525 2840 4247 4264 4417 4602 5047 Size: 1255 945 315 1407 17 153 185 445 Cuts at: 5047 325 Size: 1498 Fragments arranged by size: 1498 1407 1255 945 445 315 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2943 4466 6178 Size: 274 80 33 51 1443 966 1523 1712 Cuts at: 6178 96 Size: 138 Fragments arranged by size: 1712 1523 1443 966 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6180 6212 6180 Size: 32 6188 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6220 XbaI T'CTAG_A Cuts at: 3556 3556 Size: 6220 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2338 2338 Size: 6220 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6220 XmaI C'CCGG_G Cuts at: 1870 3545 1870 Size: 1675 4545 XmnI GAAnn'nnTTC Cuts at: 4349 4349 Size: 6220 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4028 276 Size: 53 83 186 936 2494 2468 Fragments arranged by size: 2494 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspXI PsrI PvuII RsrII SacI SanDI SapI SexAI SfiI SfoI SrfI SwaI