(Circular) (Six-base) MAPSORT of: pFN21AB0453.seq Check: 7448 from: 1 to: 6220 pFN21AB0453 6220 bp With 182 enzymes: SgfI * April 15, 2010 09:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4030 278 Size: 53 83 186 936 2494 2468 Fragments arranged by size: 2494 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2721 3563 1937 Size: 784 842 4594 Fragments arranged by size: 4594 842 784 Acc65I G'GTAC_C Cuts at: 3033 3541 3033 Size: 508 5712 AclI AA'CG_TT Cuts at: 1563 4347 4720 1563 Size: 2784 373 3063 Fragments arranged by size: 3063 2784 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2597 4283 5667 5935 1632 Size: 965 1686 1384 268 1917 Fragments arranged by size: 1917 1686 1384 965 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6023 AflIII A'CryG_T Cuts at: 1203 2163 5119 6096 1203 Size: 960 2956 977 1327 Fragments arranged by size: 2956 1327 977 960 AgeI A'CCGG_T Cuts at: 5051 5051 Size: 6220 AhdI GACnn_n'nnGTC Cuts at: 4949 4949 Size: 6220 AleI CACnn'nnGTG Cuts at: 1166 2213 1166 Size: 1047 5173 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6220 AlwNI CAG_nnn'CTG Cuts at: 3109 3121 3963 5535 3109 Size: 12 842 1572 3794 Fragments arranged by size: 3794 1572 842 12 ApaLI G'TGCA_C Cuts at: 4277 5433 4277 Size: 1156 5064 ApoI r'AATT_y Cuts at: 1493 2443 2864 3529 3755 3907 6016 1493 Size: 950 421 665 226 152 2109 1697 Fragments arranged by size: 2109 1697 950 665 421 226 152 AseI AT'TA_AT Cuts at: 160 1032 4774 160 Size: 872 3742 1606 Fragments arranged by size: 3742 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6220 AvaI C'yCGr_G Cuts at: 1870 1943 2148 2403 2945 3545 1870 Size: 73 205 255 542 600 4545 Fragments arranged by size: 4545 600 542 255 205 73 BamHI G'GATC_C Cuts at: 3550 3917 3550 Size: 367 5853 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2950 3033 3541 4996 618 Size: 325 217 627 1163 83 508 1455 1842 Fragments arranged by size: 1842 1455 1163 627 508 325 217 83 BanII G_rGCy'C Cuts at: 1391 3539 1391 Size: 2148 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5249 BbvCI CC'TCA_GC Cuts at: 3479 3479 Size: 6220 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3145 3179 4411 4445 1903 Size: 34 1208 34 1232 34 3678 Fragments arranged by size: 3678 1232 1208 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4112 5322 4112 Size: 1210 5010 BclI T'GATC_A Cuts at: 1548 1548 Size: 6220 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4831 136 Size: 107 122 71 631 605 3159 1525 Fragments arranged by size: 3159 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6215 6215 Size: 6220 BlpI GC'TnA_GC Cuts at: 3637 3637 Size: 6220 Bme1580I G_kGCm'C Cuts at: 1790 2955 3496 4281 5437 1790 Size: 1165 541 785 1156 2573 Fragments arranged by size: 2573 1165 1156 785 541 BmgBI CAC'GTC Cuts at: 1318 2743 1318 Size: 1425 4795 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3951 4909 442 Size: 416 53 83 399 24 2534 958 1753 Fragments arranged by size: 2534 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6220 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3267 3267 Size: 6220 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2531 2891 3143 4880 1239 Size: 1292 360 252 1737 2579 Fragments arranged by size: 2579 1737 1292 360 252 Bpu10I CC'TnA_GC Cuts at: 3479 3479 Size: 6220 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3262 3455 4345 5210 5508 3262 Size: 193 890 865 298 3974 Fragments arranged by size: 3974 890 865 298 193 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4883 882 Size: 617 3384 2219 Fragments arranged by size: 3384 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2164 4011 493 Size: 711 960 1847 2702 Fragments arranged by size: 2702 1847 960 711 BsaBI GATnn'nnATC Cuts at: 6214 6214 Size: 6220 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3436 4027 4409 5044 Size: 53 83 186 936 1903 591 382 635 Cuts at: 5044 275 Size: 1451 Fragments arranged by size: 1903 1451 936 635 591 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4653 5051 5325 5472 5883 4653 Size: 398 274 147 411 4990 Fragments arranged by size: 4990 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5995 Fragments arranged by size: 5995 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2306 2375 2425 1186 Size: 1120 69 50 4981 Fragments arranged by size: 4981 1120 69 50 BseYI C'CCAG_C Cuts at: 5423 5919 5423 Size: 496 5724 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3134 1163 Size: 573 1398 4249 Fragments arranged by size: 4249 1398 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3670 4431 5459 1540 Size: 275 186 1669 761 1028 2301 Fragments arranged by size: 2301 1669 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2323 4281 4366 5437 2323 Size: 1958 85 1071 3106 Fragments arranged by size: 3106 1958 1071 85 BsmI GAATG_Cn' Cuts at: 2594 3731 3824 2594 Size: 1137 93 4990 Fragments arranged by size: 4990 1137 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2323 2955 3496 3539 4281 4366 5437 Size: 399 533 632 541 43 742 85 1071 Cuts at: 5437 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 632 541 533 399 85 43 BspEI T'CCGG_A Cuts at: 5883 5883 Size: 6220 BspHI T'CATG_A Cuts at: 2730 4109 5839 2730 Size: 1379 1730 3111 Fragments arranged by size: 3111 1730 1379 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3575 844 Size: 2731 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4107 1394 Size: 429 2284 3507 Fragments arranged by size: 3507 2284 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2493 3163 4715 4889 65 Size: 1183 1245 670 1552 174 1396 Fragments arranged by size: 1552 1396 1245 1183 670 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2769 3462 4864 5051 1700 Size: 110 959 693 1402 187 2869 Fragments arranged by size: 2869 1402 959 693 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6220 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6220 BssSI C'ACGA_G Cuts at: 4280 5292 4280 Size: 1012 5208 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6220 BstBI TT'CG_AA Cuts at: 2068 3422 5024 2068 Size: 1354 1602 3264 Fragments arranged by size: 3264 1602 1354 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6220 BstXI CCAn_nnnn'nTGG Cuts at: 2509 2509 Size: 6220 BstYI r'GATC_y Cuts at: 1972 2304 2638 3550 3917 4302 4319 5771 5857 Size: 332 334 912 367 385 17 1452 86 Cuts at: 5857 5869 6215 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 912 385 367 346 334 332 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5668 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5481 Fragments arranged by size: 5481 683 56 BtsI GCAGTG_nn' Cuts at: 2969 3736 4530 4550 2969 Size: 767 794 20 4639 Fragments arranged by size: 4639 794 767 20 ClaI AT'CG_AT Cuts at: 1554 3079 1554 Size: 1525 4695 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6185 DraI TTT'AAA Cuts at: 3524 3524 Size: 6220 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5227 809 Size: 507 3911 1802 Fragments arranged by size: 3911 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3667 4556 6185 Size: 54 1425 50 275 18 1837 889 1629 Cuts at: 6185 8 Size: 43 Fragments arranged by size: 1837 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3667 1537 Size: 275 1855 4090 Fragments arranged by size: 4090 1855 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2573 2912 2997 3036 4150 1353 Size: 1220 339 85 39 1114 3423 Fragments arranged by size: 3423 1220 1114 339 85 39 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4807 5181 5327 387 Size: 1064 138 3218 374 146 1280 Fragments arranged by size: 3218 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2531 2597 2891 3143 4283 4880 5667 Size: 393 899 66 294 252 1140 597 787 Cuts at: 5667 5935 1239 Size: 268 1524 Fragments arranged by size: 1524 1140 899 787 597 393 294 268 252 66 EcoO109I rG'GnC_Cy Cuts at: 3106 3106 Size: 6220 EcoRI G'AATT_C Cuts at: 2443 3529 3907 2443 Size: 1086 378 4756 Fragments arranged by size: 4756 1086 378 EcoRV GAT'ATC Cuts at: 3083 3083 Size: 6220 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2476 2476 Size: 6220 FspI TGC'GCA Cuts at: 4726 4726 Size: 6220 HaeII r_GCGC'y Cuts at: 1678 2333 5367 1678 Size: 655 3034 2531 Fragments arranged by size: 3034 2531 655 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2667 2699 3237 3269 4861 Size: 32 135 32 1095 32 538 32 1592 Cuts at: 4861 4893 4935 4967 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1592 1095 538 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2686 3564 3810 669 Size: 988 281 748 878 246 3079 Fragments arranged by size: 3079 988 878 748 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6220 HpaI GTT'AAC Cuts at: 3810 3810 Size: 6220 KpnI G_GTAC'C Cuts at: 3037 3545 3037 Size: 508 5712 MfeI C'AATT_G Cuts at: 3819 3819 Size: 6220 MluI A'CGCG_T Cuts at: 6096 6096 Size: 6220 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5334 5518 1305 Size: 4029 184 2007 Fragments arranged by size: 4029 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4398 Fragments arranged by size: 4398 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2213 2286 2654 4178 4537 4696 518 Size: 648 1047 73 368 1524 359 159 2042 Fragments arranged by size: 2042 1524 1047 648 368 359 159 73 MspA1I CmG'CkG Cuts at: 1606 3118 3636 4313 5461 5706 1606 Size: 1512 518 677 1148 245 2120 Fragments arranged by size: 2120 1512 1148 677 518 245 NaeI GCC'GGC Cuts at: 2771 3464 2771 Size: 693 5527 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5668 NdeI CA'TA_TG Cuts at: 387 387 Size: 6220 NgoMIV G'CCGG_C Cuts at: 2769 3462 2769 Size: 693 5527 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6220 NotI GC'GGCC_GC Cuts at: 3667 3667 Size: 6220 NspI r_CATG'y Cuts at: 1132 1229 3505 3578 5123 1132 Size: 97 2276 73 1545 2229 Fragments arranged by size: 2276 2229 1545 97 73 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6220 PciI A'CATG_T Cuts at: 5119 5119 Size: 6220 PflMI CCAn_nnn'nTGG Cuts at: 1108 3109 1108 Size: 2001 4219 PfoI T'CCnGG_A Cuts at: 2694 2694 Size: 6220 PmeI GTTT'AAAC Cuts at: 3524 3524 Size: 6220 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4289 4321 5802 5834 4289 Size: 32 1481 32 4675 Fragments arranged by size: 4675 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3106 3106 Size: 6220 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6220 PsiI TTA'TAA Cuts at: 3790 3790 Size: 6220 PstI C_TGCA'G Cuts at: 830 3572 830 Size: 2742 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6220 SalI G'TCGA_C Cuts at: 1936 3562 1936 Size: 1626 4594 SbfI CC_TGCA'GG Cuts at: 3572 3572 Size: 6220 ScaI AGT'ACT Cuts at: 4468 6180 4468 Size: 1712 4508 SexAI A'CCwGG_T Cuts at: 3108 3108 Size: 6220 SfcI C'TryA_G Cuts at: 826 1045 3568 4703 5384 5575 826 Size: 219 2523 1135 681 191 1471 Fragments arranged by size: 2523 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6220 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6220 SmaI CCC'GGG Cuts at: 1872 2150 2405 3547 1872 Size: 278 255 1142 4545 Fragments arranged by size: 4545 1142 278 255 SmlI C'TyrA_G Cuts at: 820 1017 1943 3241 3470 4324 5225 5487 820 Size: 197 926 1298 229 854 901 262 1553 Fragments arranged by size: 1553 1298 926 901 854 262 229 197 SnaBI TAC'GTA Cuts at: 493 4011 493 Size: 3518 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6220 SphI G_CATG'C Cuts at: 1132 3578 1132 Size: 2446 3774 SspI AAT'ATT Cuts at: 5 52 2506 4144 5 Size: 47 2454 1638 2081 Fragments arranged by size: 2454 2081 1638 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6220 StyI C'CwwG_G Cuts at: 513 1065 3659 513 Size: 552 2594 3074 Fragments arranged by size: 3074 2594 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3279 4247 4264 4417 4602 5047 325 Size: 1255 1699 968 17 153 185 445 1498 Fragments arranged by size: 1699 1498 1255 968 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4466 6178 96 Size: 274 80 33 51 1443 2489 1712 138 Fragments arranged by size: 2489 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6180 6212 6180 Size: 32 6188 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6220 XbaI T'CTAG_A Cuts at: 3556 3556 Size: 6220 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6220 XmaI C'CCGG_G Cuts at: 1870 2148 2403 3545 1870 Size: 278 255 1142 4545 Fragments arranged by size: 4545 1142 278 255 XmnI GAAnn'nnTTC Cuts at: 2447 4349 2447 Size: 1902 4318 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4028 276 Size: 53 83 186 936 2494 2468 Fragments arranged by size: 2494 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI