(Circular) (Six-base) MAPSORT of: pFN21AB6535.seq Check: 4711 from: 1 to: 5806 pFN21AB6535 5806 bp With 182 enzymes: SgfI * April 15, 2010 09:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2276 2276 Size: 5806 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3616 278 Size: 53 83 186 936 2080 2468 Fragments arranged by size: 2468 2080 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2388 3149 1937 Size: 451 761 4594 Fragments arranged by size: 4594 761 451 Acc65I G'GTAC_C Cuts at: 3127 3127 Size: 5806 AclI AA'CG_TT Cuts at: 1563 3933 4306 1563 Size: 2370 373 3063 Fragments arranged by size: 3063 2370 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2339 3869 5253 5521 1632 Size: 707 1530 1384 268 1917 Fragments arranged by size: 1917 1530 1384 707 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5609 AflIII A'CryG_T Cuts at: 1203 4705 5682 1203 Size: 3502 977 1327 Fragments arranged by size: 3502 1327 977 AgeI A'CCGG_T Cuts at: 4637 4637 Size: 5806 AhdI GACnn_n'nnGTC Cuts at: 4535 4535 Size: 5806 AleI CACnn'nnGTG Cuts at: 1166 2291 2480 2816 3029 1166 Size: 1125 189 336 213 3943 Fragments arranged by size: 3943 1125 336 213 189 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2758 3047 1184 Size: 1574 289 3943 Fragments arranged by size: 3943 1574 289 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2519 2551 2519 Size: 32 5774 AlwNI CAG_nnn'CTG Cuts at: 2114 2615 3549 5121 2114 Size: 501 934 1572 2799 Fragments arranged by size: 2799 1572 934 501 ApaLI G'TGCA_C Cuts at: 2486 3863 5019 2486 Size: 1377 1156 3273 Fragments arranged by size: 3273 1377 1156 ApoI r'AATT_y Cuts at: 1493 2556 3115 3341 3493 5602 1493 Size: 1063 559 226 152 2109 1697 Fragments arranged by size: 2109 1697 1063 559 226 152 AseI AT'TA_AT Cuts at: 160 1032 4360 160 Size: 872 3328 1606 Fragments arranged by size: 3328 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5806 AvaI C'yCGr_G Cuts at: 1870 1943 2562 3131 1870 Size: 73 619 569 4545 Fragments arranged by size: 4545 619 569 73 BamHI G'GATC_C Cuts at: 3136 3503 3136 Size: 367 5439 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2792 3127 4582 618 Size: 325 217 627 1005 335 1455 1842 Fragments arranged by size: 1842 1455 1005 627 335 325 217 BanII G_rGCy'C Cuts at: 1391 2569 2774 3125 1391 Size: 1178 205 351 4072 Fragments arranged by size: 4072 1178 351 205 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4835 BbvCI CC'TCA_GC Cuts at: 2214 2214 Size: 5806 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3997 4031 1903 Size: 34 2060 34 3678 Fragments arranged by size: 3678 2060 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2162 3698 4908 2162 Size: 1536 1210 3060 Fragments arranged by size: 3060 1536 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5806 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2648 4417 136 Size: 107 122 71 631 605 976 1769 1525 Fragments arranged by size: 1769 1525 976 631 605 122 107 71 BglII A'GATC_T Cuts at: 5801 5801 Size: 5806 BlpI GC'TnA_GC Cuts at: 2037 2568 3223 2037 Size: 531 655 4620 Fragments arranged by size: 4620 655 531 Bme1580I G_kGCm'C Cuts at: 1790 2490 2580 3867 5023 1790 Size: 700 90 1287 1156 2573 Fragments arranged by size: 2573 1287 1156 700 90 BmgBI CAC'GTC Cuts at: 1318 2428 1318 Size: 1110 4696 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3537 4495 442 Size: 416 53 83 399 24 2120 958 1753 Fragments arranged by size: 2120 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5806 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2570 2856 2901 4466 1239 Size: 1331 286 45 1565 2579 Fragments arranged by size: 2579 1565 1331 286 45 Bpu10I CC'TnA_GC Cuts at: 2214 2214 Size: 5806 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3931 4796 5094 3931 Size: 865 298 4643 Fragments arranged by size: 4643 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2980 4469 882 Size: 617 1481 1489 2219 Fragments arranged by size: 2219 1489 1481 617 BsaAI yAC'GTr Cuts at: 493 1204 3597 493 Size: 711 2393 2702 Fragments arranged by size: 2702 2393 711 BsaBI GATnn'nnATC Cuts at: 5800 5800 Size: 5806 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3613 3995 4630 275 Size: 53 83 186 936 2080 382 635 1451 Fragments arranged by size: 2080 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2225 4239 4637 4911 5058 5469 2225 Size: 2014 398 274 147 411 2562 Fragments arranged by size: 2562 2014 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2519 2549 2850 2880 1178 Size: 30 165 30 1116 30 301 30 4104 Fragments arranged by size: 4104 1116 301 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2904 1186 Size: 1718 4088 BseYI C'CCAG_C Cuts at: 3056 5009 5505 3056 Size: 1953 496 3357 Fragments arranged by size: 3357 1953 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3053 1163 Size: 573 1317 3916 Fragments arranged by size: 3916 1317 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3256 4017 5045 1540 Size: 275 186 1255 761 1028 2301 Fragments arranged by size: 2301 1255 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2490 2774 3867 3952 5023 2490 Size: 284 1093 85 1071 3273 Fragments arranged by size: 3273 1093 1071 284 85 BsmI GAATG_Cn' Cuts at: 3317 3410 3317 Size: 93 5713 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2490 2569 2580 2774 3125 3867 3952 Size: 399 700 79 11 194 351 742 85 Cuts at: 3952 5023 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 700 399 351 194 85 79 11 BspEI T'CCGG_A Cuts at: 2225 5469 2225 Size: 3244 2562 BspHI T'CATG_A Cuts at: 3695 5425 3695 Size: 1730 4076 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2276 2673 2750 2958 3161 844 Size: 1432 397 77 208 203 3489 Fragments arranged by size: 3489 1432 397 208 203 77 BsrBI CCG'CTC Cuts at: 1394 1823 3693 1394 Size: 429 1870 3507 Fragments arranged by size: 3507 1870 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4301 4475 65 Size: 1183 3053 174 1396 Fragments arranged by size: 3053 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4450 4637 1700 Size: 110 2640 187 2869 Fragments arranged by size: 2869 2640 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5806 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5806 BssSI C'ACGA_G Cuts at: 3866 4878 3866 Size: 1012 4794 BstAPI GCAn_nnn'nTGC Cuts at: 794 2439 2732 794 Size: 1645 293 3868 Fragments arranged by size: 3868 1645 293 BstBI TT'CG_AA Cuts at: 4610 4610 Size: 5806 BstEII G'GTnAC_C Cuts at: 1184 2985 1184 Size: 1801 4005 BstXI CCAn_nnnn'nTGG Cuts at: 2293 2818 2293 Size: 525 5281 BstYI r'GATC_y Cuts at: 1972 3136 3503 3888 3905 5357 5443 5455 5801 Size: 1164 367 385 17 1452 86 12 346 Cuts at: 5801 1972 Size: 1977 Fragments arranged by size: 1977 1452 1164 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2389 2389 Size: 5806 Bsu36I CC'TnA_GG Cuts at: 2992 2992 Size: 5806 BtgI C'CryG_G Cuts at: 513 1065 2382 2817 513 Size: 552 1317 435 3502 Fragments arranged by size: 3502 1317 552 435 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2115 487 Size: 683 56 889 4178 Fragments arranged by size: 4178 889 683 56 BtsI GCAGTG_nn' Cuts at: 2742 3322 4116 4136 2742 Size: 580 794 20 4412 Fragments arranged by size: 4412 794 580 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5806 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5771 DraI TTT'AAA Cuts at: 3110 3110 Size: 5806 DraIII CAC_nnn'GTG Cuts at: 2708 2708 Size: 5806 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4813 809 Size: 507 3497 1802 Fragments arranged by size: 3497 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2508 3253 4142 Size: 54 1425 50 275 18 678 745 889 Cuts at: 4142 5771 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 745 678 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3253 1537 Size: 275 1441 4090 Fragments arranged by size: 4090 1441 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3736 1353 Size: 2383 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4393 4767 4913 387 Size: 1064 138 2804 374 146 1280 Fragments arranged by size: 2804 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2772 2772 Size: 5806 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2339 2570 2856 2901 3869 4466 5253 Size: 393 707 231 286 45 968 597 787 Cuts at: 5253 5521 1239 Size: 268 1524 Fragments arranged by size: 1524 968 787 707 597 393 286 268 231 45 EcoRI G'AATT_C Cuts at: 3115 3493 3115 Size: 378 5428 FspI TGC'GCA Cuts at: 2432 4312 2432 Size: 1880 3926 HaeII r_GCGC'y Cuts at: 1678 4953 1678 Size: 3275 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4447 4479 4521 4553 1373 Size: 32 135 32 2875 32 42 32 2626 Fragments arranged by size: 2875 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3150 3396 669 Size: 988 281 1212 246 3079 Fragments arranged by size: 3079 1212 988 281 246 HindIII A'AGCT_T Cuts at: 748 2714 748 Size: 1966 3840 HpaI GTT'AAC Cuts at: 3396 3396 Size: 5806 KpnI G_GTAC'C Cuts at: 3131 3131 Size: 5806 MfeI C'AATT_G Cuts at: 3405 3405 Size: 5806 MluI A'CGCG_T Cuts at: 5682 5682 Size: 5806 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4920 5104 1305 Size: 3615 184 2007 Fragments arranged by size: 3615 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3984 Fragments arranged by size: 3984 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2291 2480 2816 2963 2969 3029 3764 Size: 648 1125 189 336 147 6 60 735 Cuts at: 3764 4123 4282 518 Size: 359 159 2042 Fragments arranged by size: 2042 1125 735 648 359 336 189 159 147 60 6 MspA1I CmG'CkG Cuts at: 1606 2317 2356 2467 2548 2876 3222 3899 5047 Size: 711 39 111 81 328 346 677 1148 Cuts at: 5047 5292 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 711 677 346 328 245 111 81 39 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5254 NdeI CA'TA_TG Cuts at: 387 387 Size: 5806 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5806 NotI GC'GGCC_GC Cuts at: 3253 3253 Size: 5806 NspI r_CATG'y Cuts at: 1132 1229 2330 3164 4709 1132 Size: 97 1101 834 1545 2229 Fragments arranged by size: 2229 1545 1101 834 97 PasI CC'CwG_GG Cuts at: 1348 2072 1348 Size: 724 5082 PciI A'CATG_T Cuts at: 4705 4705 Size: 5806 PflMI CCAn_nnn'nTGG Cuts at: 1108 2278 1108 Size: 1170 4636 PfoI T'CCnGG_A Cuts at: 2917 2917 Size: 5806 PmeI GTTT'AAAC Cuts at: 3110 3110 Size: 5806 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2519 2551 3875 3907 5388 5420 2519 Size: 32 1324 32 1481 32 2905 Fragments arranged by size: 2905 1481 1324 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5806 PsiI TTA'TAA Cuts at: 3376 3376 Size: 5806 PstI C_TGCA'G Cuts at: 830 3158 830 Size: 2328 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5806 PvuII CAG'CTG Cuts at: 2317 2356 2548 2876 2317 Size: 39 192 328 5247 Fragments arranged by size: 5247 328 192 39 SacI G_AGCT'C Cuts at: 2774 2774 Size: 5806 SalI G'TCGA_C Cuts at: 1936 3148 1936 Size: 1212 4594 SbfI CC_TGCA'GG Cuts at: 3158 3158 Size: 5806 ScaI AGT'ACT Cuts at: 4054 5766 4054 Size: 1712 4094 SfcI C'TryA_G Cuts at: 826 1045 2136 3154 4289 4970 5161 826 Size: 219 1091 1018 1135 681 191 1471 Fragments arranged by size: 1471 1135 1091 1018 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5806 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5806 SmaI CCC'GGG Cuts at: 1872 2564 3133 1872 Size: 692 569 4545 Fragments arranged by size: 4545 692 569 SmlI C'TyrA_G Cuts at: 820 1017 1943 3910 4811 5073 820 Size: 197 926 1967 901 262 1553 Fragments arranged by size: 1967 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3597 493 Size: 3104 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5806 SphI G_CATG'C Cuts at: 1132 2330 3164 1132 Size: 1198 834 3774 Fragments arranged by size: 3774 1198 834 SspI AAT'ATT Cuts at: 5 52 3730 5 Size: 47 3678 2081 Fragments arranged by size: 3678 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5806 StyI C'CwwG_G Cuts at: 513 1065 2162 3245 513 Size: 552 1097 1083 3074 Fragments arranged by size: 3074 1097 1083 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2957 3833 3850 4003 4188 4633 325 Size: 1255 1377 876 17 153 185 445 1498 Fragments arranged by size: 1498 1377 1255 876 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4052 5764 96 Size: 274 80 33 51 1443 2075 1712 138 Fragments arranged by size: 2075 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2850 2882 5766 5798 2850 Size: 32 2884 32 2858 Fragments arranged by size: 2884 2858 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5806 XbaI T'CTAG_A Cuts at: 3142 3142 Size: 5806 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2545 2818 2545 Size: 273 5533 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5806 XmaI C'CCGG_G Cuts at: 1870 2562 3131 1870 Size: 692 569 4545 Fragments arranged by size: 4545 692 569 XmnI GAAnn'nnTTC Cuts at: 3935 3935 Size: 5806 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3614 276 Size: 53 83 186 936 2080 2468 Fragments arranged by size: 2468 2080 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI