Restriction map for
pFN21AB0447
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# NO CUT ENZYMES ( 112 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI AhdI Alw44I AocI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BalI BamHI BbrPI BbvCI
BcgI BclI BfrI BlnI BsaAI
BsaBI BseAI BsgI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrGI Bst1107I BstBI BstXI
BstZ17I Bsu36I ClaI CpoI DraI
EagI Eam1105I EclXI Eco52I Eco81I
EcoNI EcoRV EcoT22I FbaI FseI
FspI HindIII HpaI KpnI KspI
MamI MfeI MluNI MroI MscI
MunI NaeI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SbfI SexAI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tth111I Van91I XbaI XcmI
XhoI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AflIII 534 ACGCGT A^C[GA][CT]GT
AlwNI 211 CAGGTCCTG CAG[ACGT][ACGT][ACGT]^CTG
AvaI 746 CCCGGG C^[CT]CG[GA]G
BbeI 60 GGCGCC GGCGC^C
BciVI 759 GGATAC GTATCC(6/5)
BfaI 376 CTAG C^TAG
BglI 557 GCCTGGTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1076 AGATCT A^GATCT
BsiEI 651 CGACCG CG[GA][CT]^CG
BsiWI 857 CGTACG C^GTACG
BsoBI 746 CCCGGG C^[CT]CG[GA]G
BsrDI 461 GCAATG GCAATG(2/0)
BsrFI 653 ACCGGC [GA]^CCGG[CT]
BssHII 100 GCGCGC G^CGCGC
BssSI 910 CACGAG CACGAG(-5/-1)
BstAPI 108 GCACAGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I 653 ACCGGC [GA]^CCGG[CT]
DraII 212 AGGTCCT [GA]G^G[ACGT]CC[CT]
DraIII 109 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 223 GACCAGACGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 564 TGGCCG [CT]^GGCC[GA]
EcoO109I 212 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRI 973 GAATTC G^AATTC
EcoT14I 926 CCATGG C^C[AT][AT]GG
KasI 60 GGCGCC G^GCGCC
MaeI 376 CTAG C^TAG
MluI 534 ACGCGT A^CGCGT
MseI 1253 TTAA T^TAA
NarI 60 GGCGCC GG^CGCC
NcoI 926 CCATGG C^CATGG
NspI 668 ACATGC [GA]CATG^[CT]
PpuMI 212 AGGTCCT [GA]G^G[AT]CC[CT]
PvuII 1237 CAGCTG CAG^CTG
ScaI 1298 AGTACT AGT^ACT
SfiI 556 GGCCTGGTTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 60 GGCGCC GGC^GCC
SmaI 746 CCCGGG CCC^GGG
StyI 926 CCATGG C^C[AT][AT]GG
Tru9I 1253 TTAA T^TAA
XmaCI 746 CCCGGG C^CCGGG
XmaI 746 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcyI 60 GGCGCC G[GA]^CG[CT]C
84 GGCGTC
AlwI 1017 GATCC GGATC(4/5)
1393 GATCC
Aor51HI 46 AGCGCT AGC^GCT
1467 AGCGCT
BanI 60 GGCGCC G^G[CT][GA]CC
114 GGTGCC
BbsI 162 GAAGAC GAAGAC(2/6)
1291 GAAGAC
BpuAI 162 GAAGAC GAAGAC(2/6)
1291 GAAGAC
BsaHI 60 GGCGCC G[GA]^CG[CT]C
84 GGCGTC
BstEII 622 GGTGACC G^GT[ACGT]ACC
1311 GGTCACC
BstPI 622 GGTGACC G^GT[ACGT]ACC
1311 GGTCACC
BstYI 1016 AGATCC [GA]^GATC[CT]
1076 AGATCT
Eco47III 46 AGCGCT AGC^GCT
1467 AGCGCT
EcoO65I 622 GGTGACC G^GT[ACGT]ACC
1311 GGTCACC
Hin1I 60 GGCGCC G[GA]^CG[CT]C
84 GGCGTC
HincII 304 GTCAAC GT[CT]^[GA]AC
1164 GTTGAC
HindII 304 GTCAAC GT[CT]^[GA]AC
1164 GTTGAC
MaeII 278 ACGT A^CGT
1346 ACGT
MflI 1016 AGATCC [GA]^GATC[CT]
1076 AGATCT
MspA1I 118 CCGCTG C[AC]G^C[GT]G
1237 CAGCTG
PstI 540 CTGCAG CTGCA^G
1471 CTGCAG
SapI 349 GAAGAGC GCTCTTC(1/4)
723 GCTCTTC
SmlI 574 CTTGAG C^T[CT][GA]AG
898 CTTGAG
StuI 555 AGGCCT AGG^CCT
799 AGGCCT
TaqI 632 TCGA T^CGA
1136 TCGA
TfiI 424 GAATC G^A[AT]TC
1141 GAATC
TthHB8I 632 TCGA T^CGA
1136 TCGA
XhoII 1016 AGATCC [GA]^GATC[CT]
1076 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
ApoI AspHI AvaII BanII BbvI
BcnI BlpI BmyI BpmI Bpu1102I
BsaI BsaJI BsaWI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstUI Cac8I CelII CfoI Cfr13I
DdeI DpnII DsaI EarI EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeIII
MboI MboII MnlI MslI MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PleI RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
TseI Tsp45I Tsp509I TspRI