(Circular) (Six-base) MAPSORT of: pFN21AB0447.seq Check: 7554 from: 1 to: 6208 pFN21AB0447 6208 bp With 182 enzymes: SgfI * April 15, 2010 09:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4018 278 Size: 53 83 186 936 2482 2468 Fragments arranged by size: 2482 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3551 1937 Size: 1614 4594 Acc65I G'GTAC_C Cuts at: 3529 3529 Size: 6208 AclI AA'CG_TT Cuts at: 1563 4335 4708 1563 Size: 2772 373 3063 Fragments arranged by size: 3063 2772 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2465 2683 2770 4271 5655 5923 1632 Size: 833 218 87 1501 1384 268 1917 Fragments arranged by size: 1917 1501 1384 833 268 218 87 AfeI AGC'GCT Cuts at: 2053 3474 2053 Size: 1421 4787 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6011 AflIII A'CryG_T Cuts at: 1203 2539 5107 6084 1203 Size: 1336 2568 977 1327 Fragments arranged by size: 2568 1336 1327 977 AgeI A'CCGG_T Cuts at: 5039 5039 Size: 6208 AhdI GACnn_n'nnGTC Cuts at: 4937 4937 Size: 6208 AleI CACnn'nnGTG Cuts at: 1166 3323 3371 1166 Size: 2157 48 4003 Fragments arranged by size: 4003 2157 48 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2384 2560 2864 1184 Size: 1200 176 304 4528 Fragments arranged by size: 4528 1200 304 176 AlwNI CAG_nnn'CTG Cuts at: 2221 3951 5523 2221 Size: 1730 1572 2906 Fragments arranged by size: 2906 1730 1572 ApaLI G'TGCA_C Cuts at: 4265 5421 4265 Size: 1156 5052 ApoI r'AATT_y Cuts at: 1493 2295 2978 3446 3517 3743 3895 6004 1493 Size: 802 683 468 71 226 152 2109 1697 Fragments arranged by size: 2109 1697 802 683 468 226 152 71 AseI AT'TA_AT Cuts at: 160 1032 4762 160 Size: 872 3730 1606 Fragments arranged by size: 3730 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6208 AvaI C'yCGr_G Cuts at: 1870 1943 2751 3533 1870 Size: 73 808 782 4545 Fragments arranged by size: 4545 808 782 73 BamHI G'GATC_C Cuts at: 3538 3905 3538 Size: 367 5841 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2065 2119 3529 4984 618 Size: 325 217 627 278 54 1410 1455 1842 Fragments arranged by size: 1842 1455 1410 627 325 278 217 54 BanII G_rGCy'C Cuts at: 1391 2253 2454 2603 2730 2760 2871 3141 3527 Size: 862 201 149 127 30 111 270 386 Cuts at: 3527 1391 Size: 4072 Fragments arranged by size: 4072 862 386 270 201 149 127 111 30 BbeI G_GCGC'C Cuts at: 2069 2069 Size: 6208 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2174 3303 928 Size: 971 275 1129 3833 Fragments arranged by size: 3833 1129 971 275 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4399 4433 1903 Size: 34 50 34 2378 34 3678 Fragments arranged by size: 3678 2378 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2758 4100 5310 2758 Size: 1342 1210 3656 Fragments arranged by size: 3656 1342 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6208 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2568 4819 136 Size: 107 122 71 631 605 896 2251 1525 Fragments arranged by size: 2251 1525 896 631 605 122 107 71 BglII A'GATC_T Cuts at: 3081 6203 3081 Size: 3122 3086 BlpI GC'TnA_GC Cuts at: 2372 2922 3123 3625 2372 Size: 550 201 502 4955 Fragments arranged by size: 4955 550 502 201 Bme1580I G_kGCm'C Cuts at: 1790 2080 2115 4269 5425 1790 Size: 290 35 2154 1156 2573 Fragments arranged by size: 2573 2154 1156 290 35 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6208 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2067 3271 3939 Size: 416 53 83 399 24 650 1204 668 Cuts at: 3939 4897 442 Size: 958 1753 Fragments arranged by size: 1753 1204 958 668 650 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6208 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2131 2237 2669 3137 3257 3415 4868 1239 Size: 892 106 432 468 120 158 1453 2579 Fragments arranged by size: 2579 1453 892 468 432 158 120 106 Bpu10I CC'TnA_GC Cuts at: 2557 2557 Size: 6208 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2600 2924 4333 5198 5496 2600 Size: 324 1409 865 298 3312 Fragments arranged by size: 3312 1409 865 324 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2242 2414 2465 3449 4871 882 Size: 617 743 172 51 984 1422 2219 Fragments arranged by size: 2219 1422 984 743 617 172 51 BsaAI yAC'GTr Cuts at: 493 1204 3999 493 Size: 711 2795 2702 Fragments arranged by size: 2795 2702 711 BsaBI GATnn'nnATC Cuts at: 6202 6202 Size: 6208 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2066 2090 4015 4397 Size: 53 83 186 936 533 24 1925 382 Cuts at: 4397 5032 275 Size: 635 1451 Fragments arranged by size: 1925 1451 936 635 533 382 186 83 53 24 BsaWI w'CCGG_w Cuts at: 2799 3003 3359 4641 5039 5313 5460 5871 2799 Size: 204 356 1282 398 274 147 411 3136 Fragments arranged by size: 3136 1282 411 398 356 274 204 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2053 2083 3071 3098 3101 Size: 30 165 30 650 30 988 27 3 Cuts at: 3101 3128 3212 3242 1178 Size: 27 84 30 4144 Fragments arranged by size: 4144 988 650 165 84 30 30 30 30 27 27 3 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2115 2597 3217 3518 1186 Size: 929 482 620 301 3876 Fragments arranged by size: 3876 929 620 482 301 BseYI C'CCAG_C Cuts at: 5411 5907 5411 Size: 496 5712 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5635 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2659 3658 4419 5447 1540 Size: 275 186 658 999 761 1028 2301 Fragments arranged by size: 2301 1028 999 761 658 275 186 BsiHKAI G_wGCw'C Cuts at: 2253 2454 2603 2730 3141 4269 4354 5425 2253 Size: 201 149 127 411 1128 85 1071 3036 Fragments arranged by size: 3036 1128 1071 411 201 149 127 85 BsiWI C'GTAC_G Cuts at: 2862 2862 Size: 6208 BsmI GAATG_Cn' Cuts at: 3719 3812 3719 Size: 93 6115 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2080 2115 2253 2454 2603 2730 2760 Size: 399 290 35 138 201 149 127 30 Cuts at: 2760 2871 3141 3527 4269 4354 5425 1391 Size: 111 270 386 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 386 290 270 201 149 138 127 111 85 35 30 BspEI T'CCGG_A Cuts at: 5871 5871 Size: 6208 BspHI T'CATG_A Cuts at: 4097 5827 4097 Size: 1730 4478 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 1999 2206 2528 2787 3563 844 Size: 1155 207 322 259 776 3489 Fragments arranged by size: 3489 1155 776 322 259 207 BsrBI CCG'CTC Cuts at: 1394 1823 4095 1394 Size: 429 2272 3507 Fragments arranged by size: 3507 2272 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2473 4703 4877 65 Size: 1183 1225 2230 174 1396 Fragments arranged by size: 2230 1396 1225 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2658 4852 5039 1700 Size: 110 848 2194 187 2869 Fragments arranged by size: 2869 2194 848 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6208 BssHII G'CGCG_C Cuts at: 1925 2105 1925 Size: 180 6028 BssSI C'ACGA_G Cuts at: 2915 4268 5280 2915 Size: 1353 1012 3843 Fragments arranged by size: 3843 1353 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2119 794 Size: 1325 4883 BstBI TT'CG_AA Cuts at: 5012 5012 Size: 6208 BstEII G'GTnAC_C Cuts at: 1184 2627 3316 1184 Size: 1443 689 4076 Fragments arranged by size: 4076 1443 689 BstYI r'GATC_y Cuts at: 1972 3021 3081 3538 3905 4290 4307 5759 5845 Size: 1049 60 457 367 385 17 1452 86 Cuts at: 5845 5857 6203 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1049 457 385 367 346 86 60 17 12 BtgI C'CryG_G Cuts at: 513 1065 2931 3036 3321 3459 513 Size: 552 1866 105 285 138 3262 Fragments arranged by size: 3262 1866 552 285 138 105 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5469 Fragments arranged by size: 5469 683 56 BtsI GCAGTG_nn' Cuts at: 2366 3724 4518 4538 2366 Size: 1358 794 20 4036 Fragments arranged by size: 4036 1358 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6208 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6173 DraI TTT'AAA Cuts at: 3512 3512 Size: 6208 DraIII CAC_nnn'GTG Cuts at: 2119 2119 Size: 6208 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2234 5215 809 Size: 507 918 2981 1802 Fragments arranged by size: 2981 1802 918 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2569 3655 4544 Size: 54 1425 50 275 18 739 1086 889 Cuts at: 4544 6173 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1086 889 739 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3655 1537 Size: 275 1843 4090 Fragments arranged by size: 4090 1843 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2349 2487 2735 4138 1353 Size: 996 138 248 1403 3423 Fragments arranged by size: 3423 1403 996 248 138 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3014 3479 4795 5169 5315 387 Size: 1064 138 1425 465 1316 374 146 1280 Fragments arranged by size: 1425 1316 1280 1064 465 374 146 138 EcoICRI GAG'CTC Cuts at: 2251 2452 2601 2728 3139 2251 Size: 201 149 127 411 5320 Fragments arranged by size: 5320 411 201 149 127 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2131 2237 2465 2669 2683 2770 3137 Size: 393 499 106 228 204 14 87 367 Cuts at: 3137 3257 3415 4271 4868 5655 5923 1239 Size: 120 158 856 597 787 268 1524 Fragments arranged by size: 1524 856 787 597 499 393 367 268 228 204 158 120 106 87 14 EcoO109I rG'GnC_Cy Cuts at: 2218 2218 Size: 6208 EcoRI G'AATT_C Cuts at: 2978 3517 3895 2978 Size: 539 378 5291 Fragments arranged by size: 5291 539 378 FspI TGC'GCA Cuts at: 4714 4714 Size: 6208 HaeII r_GCGC'y Cuts at: 1678 2055 2069 3476 5355 1678 Size: 377 14 1407 1879 2531 Fragments arranged by size: 2531 1879 1407 377 14 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2155 2187 2317 2349 3071 Size: 32 135 32 583 32 130 32 722 Cuts at: 3071 3103 4849 4881 4923 4955 1373 Size: 32 1746 32 42 32 2626 Fragments arranged by size: 2626 1746 722 583 135 130 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2311 3171 3552 3798 669 Size: 988 281 373 860 381 246 3079 Fragments arranged by size: 3079 988 860 381 373 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6208 HpaI GTT'AAC Cuts at: 3798 3798 Size: 6208 KasI G'GCGC_C Cuts at: 2065 2065 Size: 6208 KpnI G_GTAC'C Cuts at: 3533 3533 Size: 6208 MfeI C'AATT_G Cuts at: 3807 3807 Size: 6208 MluI A'CGCG_T Cuts at: 2539 6084 2539 Size: 3545 2663 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5322 5506 1305 Size: 4017 184 2007 Fragments arranged by size: 4017 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4386 Fragments arranged by size: 4386 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3323 3371 3407 4166 4525 4684 518 Size: 648 2157 48 36 759 359 159 2042 Fragments arranged by size: 2157 2042 759 648 359 159 48 36 MspA1I CmG'CkG Cuts at: 1606 2125 3244 3624 4301 5449 5694 1606 Size: 519 1119 380 677 1148 245 2120 Fragments arranged by size: 2120 1148 1119 677 519 380 245 NarI GG'CG_CC Cuts at: 2066 2066 Size: 6208 NcoI C'CATG_G Cuts at: 513 1065 2931 513 Size: 552 1866 3790 Fragments arranged by size: 3790 1866 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6208 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6208 NotI GC'GGCC_GC Cuts at: 3655 3655 Size: 6208 NspI r_CATG'y Cuts at: 1132 1229 2677 3566 5111 1132 Size: 97 1448 889 1545 2229 Fragments arranged by size: 2229 1545 1448 889 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6208 PciI A'CATG_T Cuts at: 5107 5107 Size: 6208 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6208 PfoI T'CCnGG_A Cuts at: 2253 2733 3431 2253 Size: 480 698 5030 Fragments arranged by size: 5030 698 480 PmeI GTTT'AAAC Cuts at: 3512 3512 Size: 6208 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2585 2617 4277 4309 5790 5822 2585 Size: 32 1660 32 1481 32 2971 Fragments arranged by size: 2971 1660 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2218 2218 Size: 6208 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6208 PsiI TTA'TAA Cuts at: 3778 3778 Size: 6208 PstI C_TGCA'G Cuts at: 830 2549 3480 3560 830 Size: 1719 931 80 3478 Fragments arranged by size: 3478 1719 931 80 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6208 PvuII CAG'CTG Cuts at: 3244 3244 Size: 6208 SacI G_AGCT'C Cuts at: 2253 2454 2603 2730 3141 2253 Size: 201 149 127 411 5320 Fragments arranged by size: 5320 411 201 149 127 SalI G'TCGA_C Cuts at: 1936 3550 1936 Size: 1614 4594 SapI GCTCTTCn'nnn_ Cuts at: 2349 2735 2349 Size: 386 5822 SbfI CC_TGCA'GG Cuts at: 3560 3560 Size: 6208 ScaI AGT'ACT Cuts at: 3305 4456 6168 3305 Size: 1151 1712 3345 Fragments arranged by size: 3345 1712 1151 SfcI C'TryA_G Cuts at: 826 1045 2545 3420 3476 3556 4691 5372 5563 Size: 219 1500 875 56 80 1135 681 191 Cuts at: 5563 826 Size: 1471 Fragments arranged by size: 1500 1471 1135 875 681 219 191 80 56 SfiI GGCCn_nnn'nGGCC Cuts at: 2568 2568 Size: 6208 SfoI GGC'GCC Cuts at: 2067 2067 Size: 6208 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6208 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6208 SmaI CCC'GGG Cuts at: 1872 2753 3535 1872 Size: 881 782 4545 Fragments arranged by size: 4545 881 782 SmlI C'TyrA_G Cuts at: 820 1017 1943 2579 2903 4312 5213 5475 820 Size: 197 926 636 324 1409 901 262 1553 Fragments arranged by size: 1553 1409 926 901 636 324 262 197 SnaBI TAC'GTA Cuts at: 493 3999 493 Size: 3506 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6208 SphI G_CATG'C Cuts at: 1132 3566 1132 Size: 2434 3774 SspI AAT'ATT Cuts at: 5 52 4132 5 Size: 47 4080 2081 Fragments arranged by size: 4080 2081 47 StuI AGG'CCT Cuts at: 1517 2562 2806 1517 Size: 1045 244 4919 Fragments arranged by size: 4919 1045 244 StyI C'CwwG_G Cuts at: 513 1065 2931 3647 513 Size: 552 1866 716 3074 Fragments arranged by size: 3074 1866 716 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2144 3253 4235 4252 4405 4590 5035 Size: 1255 564 1109 982 17 153 185 445 Cuts at: 5035 325 Size: 1498 Fragments arranged by size: 1498 1255 1109 982 564 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3303 4454 6166 Size: 274 80 33 51 1443 1326 1151 1712 Cuts at: 6166 96 Size: 138 Fragments arranged by size: 1712 1443 1326 1151 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2611 2643 6168 6200 2611 Size: 32 3525 32 2619 Fragments arranged by size: 3525 2619 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6208 XbaI T'CTAG_A Cuts at: 3544 3544 Size: 6208 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6208 XmaI C'CCGG_G Cuts at: 1870 2751 3533 1870 Size: 881 782 4545 Fragments arranged by size: 4545 881 782 XmnI GAAnn'nnTTC Cuts at: 4337 4337 Size: 6208 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4016 276 Size: 53 83 186 936 2482 2468 Fragments arranged by size: 2482 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI BsmBI BstXI BstZ17I Bsu36I EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SrfI SwaI XcmI