Restriction map for
pFN21AB0447
(Linear) (Six-base) MAPSORT of: KIBB0447.seq Check: 4737 from: 1 to: 1503
KIBB0447.orf
With 182 enzymes: SgfI *
October 7, 2009 14:13 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 460 678 765 1503
Size: 460 218 87 738
Fragments arranged by size:
738 460 218 87
AfeI AGC'GCT
Cuts at: 0 48 1469 1503
Size: 48 1421 34
Fragments arranged by size:
1421 48 34
AflIII A'CryG_T
Cuts at: 0 534 1503
Size: 534 969
AleI CACnn'nnGTG
Cuts at: 0 1318 1366 1503
Size: 1318 48 137
Fragments arranged by size:
1318 137 48
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 379 555 859 1503
Size: 379 176 304 644
Fragments arranged by size:
644 379 304 176
AlwNI CAG_nnn'CTG
Cuts at: 0 216 1503
Size: 216 1287
ApoI r'AATT_y
Cuts at: 0 290 973 1441 1503
Size: 290 683 468 62
Fragments arranged by size:
683 468 290 62
AvaI C'yCGr_G
Cuts at: 0 746 1503
Size: 746 757
BanI G'GyrC_C
Cuts at: 0 60 114 1503
Size: 60 54 1389
Fragments arranged by size:
1389 60 54
BanII G_rGCy'C
Cuts at: 0 248 449 598 725 755 866 1136 1503
Size: 248 201 149 127 30 111 270 367
Fragments arranged by size:
367 270 248 201 149 127 111 30
BbeI G_GCGC'C
Cuts at: 0 64 1503
Size: 64 1439
BbsI GAAGACnn'nnnn_
Cuts at: 0 169 1298 1503
Size: 169 1129 205
Fragments arranged by size:
1129 205 169
BciVI GTATCCnnnnn_n'
Cuts at: 0 753 1503
Size: 753 750
BglI GCCn_nnn'nGGC
Cuts at: 0 563 1503
Size: 563 940
BglII A'GATC_T
Cuts at: 0 1076 1503
Size: 1076 427
BlpI GC'TnA_GC
Cuts at: 0 367 917 1118 1503
Size: 367 550 201 385
Fragments arranged by size:
550 385 367 201
Bme1580I G_kGCm'C
Cuts at: 0 75 110 1503
Size: 75 35 1393
Fragments arranged by size:
1393 75 35
BmrI ACTGGGnnnn_n'
Cuts at: 0 62 1266 1503
Size: 62 1204 237
Fragments arranged by size:
1204 237 62
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 126 232 664 1132 1252 1410 1503
Size: 126 106 432 468 120 158 93
Fragments arranged by size:
468 432 158 126 120 106 93
Bpu10I CC'TnA_GC
Cuts at: 0 552 1503
Size: 552 951
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 595 919 1503
Size: 595 324 584
Fragments arranged by size:
595 584 324
BsaI GGTCTCn'nnnn_
Cuts at: 0 237 409 460 1444 1503
Size: 237 172 51 984 59
Fragments arranged by size:
984 237 172 59 51
BsaHI Gr'CG_yC
Cuts at: 0 61 85 1503
Size: 61 24 1418
Fragments arranged by size:
1418 61 24
BsaWI w'CCGG_w
Cuts at: 0 794 998 1354 1503
Size: 794 204 356 149
Fragments arranged by size:
794 356 204 149
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 48 78 1066 1093 1096 1123 1207 1237
Size: 48 30 988 27 3 27 84 30
Cuts at: 1237 1503
Size: 266
Fragments arranged by size:
988 266 84 48 30 30 27 27
3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 110 592 1212 1503
Size: 110 482 620 291
Fragments arranged by size:
620 482 291 110
BsiEI CG_ry'CG
Cuts at: 0 654 1503
Size: 654 849
BsiHKAI G_wGCw'C
Cuts at: 0 248 449 598 725 1136 1503
Size: 248 201 149 127 411 367
Fragments arranged by size:
411 367 248 201 149 127
BsiWI C'GTAC_G
Cuts at: 0 857 1503
Size: 857 646
Bsp1286I G_dGCh'C
Cuts at: 0 75 110 248 449 598 725 755 866
Size: 75 35 138 201 149 127 30 111
Cuts at: 866 1136 1503
Size: 270 367
Fragments arranged by size:
367 270 201 149 138 127 111 75
35 30
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 201 523 782 1503
Size: 201 322 259 721
Fragments arranged by size:
721 322 259 201
BsrDI GCAATG_nn'
Cuts at: 0 468 1503
Size: 468 1035
BsrFI r'CCGG_y
Cuts at: 0 653 1503
Size: 653 850
BssHII G'CGCG_C
Cuts at: 0 100 1503
Size: 100 1403
BssSI C'ACGA_G
Cuts at: 0 910 1503
Size: 910 593
BstAPI GCAn_nnn'nTGC
Cuts at: 0 114 1503
Size: 114 1389
BstEII G'GTnAC_C
Cuts at: 0 622 1311 1503
Size: 622 689 192
Fragments arranged by size:
689 622 192
BstYI r'GATC_y
Cuts at: 0 1016 1076 1503
Size: 1016 60 427
Fragments arranged by size:
1016 427 60
BtgI C'CryG_G
Cuts at: 0 926 1031 1316 1454 1503
Size: 926 105 285 138 49
Fragments arranged by size:
926 285 138 105 49
BtsI GCAGTG_nn'
Cuts at: 0 361 1503
Size: 361 1142
DraIII CAC_nnn'GTG
Cuts at: 0 114 1503
Size: 114 1389
DrdI GACnn_nn'nnGTC
Cuts at: 0 229 1503
Size: 229 1274
EaeI y'GGCC_r
Cuts at: 0 564 1503
Size: 564 939
EarI CTCTTCn'nnn_
Cuts at: 0 344 482 730 1503
Size: 344 138 248 773
Fragments arranged by size:
773 344 248 138
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1009 1474 1503
Size: 1009 465 29
Fragments arranged by size:
1009 465 29
EcoICRI GAG'CTC
Cuts at: 0 246 447 596 723 1134 1503
Size: 246 201 149 127 411 369
Fragments arranged by size:
411 369 246 201 149 127
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 126 232 460 664 678 765 1132 1252
Size: 126 106 228 204 14 87 367 120
Cuts at: 1252 1410 1503
Size: 158 93
Fragments arranged by size:
367 228 204 158 126 120 106 93
87 14
EcoO109I rG'GnC_Cy
Cuts at: 0 213 1503
Size: 213 1290
EcoRI G'AATT_C
Cuts at: 0 973 1503
Size: 973 530
HaeII r_GCGC'y
Cuts at: 0 50 64 1471 1503
Size: 50 14 1407 32
Fragments arranged by size:
1407 50 32 14
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 150 182 312 344 1066 1098 1503
Size: 150 32 130 32 722 32 405
Fragments arranged by size:
722 405 150 130 32 32 32
HincII GTy'rAC
Cuts at: 0 306 1166 1503
Size: 306 860 337
Fragments arranged by size:
860 337 306
KasI G'GCGC_C
Cuts at: 0 60 1503
Size: 60 1443
MluI A'CGCG_T
Cuts at: 0 534 1503
Size: 534 969
MslI CAynn'nnrTG
Cuts at: 0 1318 1366 1402 1503
Size: 1318 48 36 101
Fragments arranged by size:
1318 101 48 36
MspA1I CmG'CkG
Cuts at: 0 120 1239 1503
Size: 120 1119 264
Fragments arranged by size:
1119 264 120
NarI GG'CG_CC
Cuts at: 0 61 1503
Size: 61 1442
NcoI C'CATG_G
Cuts at: 0 926 1503
Size: 926 577
NspI r_CATG'y
Cuts at: 0 672 1503
Size: 672 831
PfoI T'CCnGG_A
Cuts at: 0 248 728 1426 1503
Size: 248 480 698 77
Fragments arranged by size:
698 480 248 77
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 580 612 1503
Size: 580 32 891
Fragments arranged by size:
891 580 32
PpuMI rG'GwC_Cy
Cuts at: 0 213 1503
Size: 213 1290
PstI C_TGCA'G
Cuts at: 0 544 1475 1503
Size: 544 931 28
Fragments arranged by size:
931 544 28
PvuII CAG'CTG
Cuts at: 0 1239 1503
Size: 1239 264
SacI G_AGCT'C
Cuts at: 0 248 449 598 725 1136 1503
Size: 248 201 149 127 411 367
Fragments arranged by size:
411 367 248 201 149 127
SapI GCTCTTCn'nnn_
Cuts at: 0 344 730 1503
Size: 344 386 773
Fragments arranged by size:
773 386 344
ScaI AGT'ACT
Cuts at: 0 1300 1503
Size: 1300 203
SfcI C'TryA_G
Cuts at: 0 540 1415 1471 1503
Size: 540 875 56 32
Fragments arranged by size:
875 540 56 32
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 563 1503
Size: 563 940
SfoI GGC'GCC
Cuts at: 0 62 1503
Size: 62 1441
SmaI CCC'GGG
Cuts at: 0 748 1503
Size: 748 755
SmlI C'TyrA_G
Cuts at: 0 574 898 1503
Size: 574 324 605
Fragments arranged by size:
605 574 324
StuI AGG'CCT
Cuts at: 0 557 801 1503
Size: 557 244 702
Fragments arranged by size:
702 557 244
StyI C'CwwG_G
Cuts at: 0 926 1503
Size: 926 577
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 139 1248 1503
Size: 139 1109 255
Fragments arranged by size:
1109 255 139
TatI w'GTAC_w
Cuts at: 0 1298 1503
Size: 1298 205
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 606 638 1503
Size: 606 32 865
Fragments arranged by size:
865 606 32
XmaI C'CCGG_G
Cuts at: 0 746 1503
Size: 746 757
Enzymes that do cut:
AcuI AfeI AflIII AleI AlfI AlwNI ApoI AvaI
BanI BanII BbeI BbsI BciVI BglI BglII BlpI
Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI
BsaXI BseRI BsiEI BsiHKAI BsiWI Bsp1286I BspMI BsrDI
BsrFI BssHII BssSI BstAPI BstEII BstYI BtgI BtsI
DraIII DrdI EaeI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI HaeII Hin4I HincII KasI MluI MslI MspA1I
NarI NcoI NspI PfoI PpiI PpuMI PstI PvuII
SacI SapI ScaI SfcI SfiI SfoI SmaI SmlI
StuI StyI TaqII TatI TstI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AgeI AhdI
AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BbvCI BcgI BclI BfrBI BmgBI BmtI BplI
BsaAI BsaBI BseYI BsgI BsmI BsmBI BspEI BspHI
BsrBI BsrGI BstBI BstXI BstZ17I Bsu36I BtgZI ClaI
CspCI DraI EagI EcoNI EcoRV FalI FseI FspI
FspAI HindIII HpaI KpnI MfeI MmeI MscI NaeI
NdeI NgoMIV NheI NotI NruI NsiI PacI PasI
PciI PflMI PmeI PmlI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacII SalI SanDI SbfI SexAI
SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI
Tth111I XbaI XcmI XhoI XmnI ZraI