Restriction map for
pFN21AB6523
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# NO CUT ENZYMES ( 149 enzymes) #
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AatII Acc65I AccIII AclI AcyI
AflII AgeI AhdI AlwNI AocI
Aor51HI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AvrII
BalI BamHI BanI BbeI BbrPI
BbvCI BcgI BciVI BfrI BglI
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsiWI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I Bsu36I CelII
ClaI CpoI DraI DraII DrdI
DsaI Eam1105I Eco47III Eco81I EcoO109I
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FseI HaeII HgaI Hin1I HindIII
HpaI KasI KpnI KspI MamI
MfeI MluI MluNI MroI MscI
MseI MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI TaqI
TfiI Tru9I Tth111I TthHB8I XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccI 1039 GTCTAC GT^[AC][GT]AC
AcsI 271 AAATTC [GA]^AATT[CT]
AfaI 483 GTAC GT^AC
AflIII 924 ACGTGT A^C[GA][CT]GT
Alw44I 928 GTGCAC G^TGCAC
ApaI 172 GGGCCC GGGCC^C
ApaLI 928 GTGCAC G^TGCAC
ApoI 271 AAATTC [GA]^AATT[CT]
AspHI 928 GTGCAC G[AT]GC[AT]^C
AviII 542 TGCGCA TGC^GCA
BbsI 702 GAAGAC GAAGAC(2/6)
BclI 554 TGATCA T^GATCA
BfaI 1081 CTAG C^TAG
BglII 498 AGATCT A^GATCT
BpuAI 702 GAAGAC GAAGAC(2/6)
BsgI 1003 GTGCAG GTGCAG(16/14)
BsiEI 1047 CGGCCG CG[GA][CT]^CG
BsiHKAI 928 GTGCAC G[AT]GC[AT]^C
BsmBI 1013 GAGACG CGTCTC(1/5)
BsrDI 518 GCAATG GCAATG(2/0)
BsrFI 1045 ACCGGC [GA]^CCGG[CT]
BsrI 547 ACTGG ACTGG(1/-1)
BstXI 138 CCAGGCCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 1021 GCGGGC GC[ACGT]^[ACGT]GC
CfoI 543 GCGC GCG^C
Cfr10I 1045 ACCGGC [GA]^CCGG[CT]
DraIII 931 CACCGCGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 1047 CGGCCG C^GGCCG
EclXI 1047 CGGCCG C^GGCCG
Eco52I 1047 CGGCCG C^GGCCG
EcoNI 780 CCTGGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 554 TGATCA T^GATCA
FspI 542 TGCGCA TGC^GCA
HhaI 543 GCGC GCG^C
HinP1I 543 GCGC G^CGC
HincII 253 GTCAAC GT[CT]^[GA]AC
HindII 253 GTCAAC GT[CT]^[GA]AC
HphI 552 GGTGA GGTGA(8/7)
MaeI 1081 CTAG C^TAG
MslI 546 CACTGGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 70 CAGCGG C[AC]G^C[GT]G
PflMI 993 CCAGATCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RsaI 483 GTAC GT^AC
SexAI 435 ACCTGGT A^CC[AT]GGT
SfaNI 416 GATGC GCATC(5/9)
SmlI 982 CTCAAG C^T[CT][GA]AG
Tsp45I 937 GTGAC ^GT[GC]AC
Van91I 993 CCAGATCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1080 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 12 enzymes) #
###################################
AccII 934 CGCG CG^CG
961 CGCG
AluI 162 AGCT AG^CT
248 AGCT
AvaII 909 GGTCC G^G[AT]CC
990 GGACC
BanII 133 GAGCCC G[GA]GC[CT]^C
172 GGGCCC
BcnI 320 CCCGG CC^[GC]GG
912 CCGGG
BstUI 934 CGCG CG^CG
961 CGCG
EaeI 638 CGGCCA [CT]^GGCC[GA]
1047 CGGCCG
MaeII 924 ACGT A^CGT
1031 ACGT
MaeIII 84 GTTAC ^GT[ACGT]AC
937 GTGAC
MvnI 934 CGCG CG^CG
961 CGCG
NciI 320 CCCGG CC^[GC]GG
912 CCGGG
Tsp509I 272 AATT ^AATT
1089 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AciI AlwI BbvI BmyI BpmI
BsaJI BseRI BsiYI BslI BsmAI
BsmFI Bsp1286I BssKI BstF5I BstNI
BstYI Cfr13I DdeI DpnII EarI
EcoRII FauI Fnu4HI FokI HaeIII
HapII HinfI HpaII ItaI Ksp632I
MboI MboII MflI MnlI MspI
MvaI MwoI NdeII NlaIII NlaIV
PleI Sau3AI Sau96I ScrFI SfcI
TseI TspRI XhoII