(Circular) (Six-base) MAPSORT of: pFN21AB6523.seq Check: 8137 from: 1 to: 5797 pFN21AB6523 5797 bp With 182 enzymes: SgfI * April 15, 2010 09:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3607 278 Size: 53 83 186 936 2071 2468 Fragments arranged by size: 2468 2071 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3045 3140 1937 Size: 1108 95 4594 Fragments arranged by size: 4594 1108 95 Acc65I G'GTAC_C Cuts at: 3118 3118 Size: 5797 AclI AA'CG_TT Cuts at: 1563 3924 4297 1563 Size: 2361 373 3063 Fragments arranged by size: 3063 2361 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2703 3860 5244 5512 1632 Size: 1071 1157 1384 268 1917 Fragments arranged by size: 1917 1384 1157 1071 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5600 AflIII A'CryG_T Cuts at: 1203 2929 4696 5673 1203 Size: 1726 1767 977 1327 Fragments arranged by size: 1767 1726 1327 977 AgeI A'CCGG_T Cuts at: 4628 4628 Size: 5797 AhdI GACnn_n'nnGTC Cuts at: 4526 4526 Size: 5797 AleI CACnn'nnGTG Cuts at: 1166 2555 1166 Size: 1389 4408 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5797 AlwNI CAG_nnn'CTG Cuts at: 3540 5112 3540 Size: 1572 4225 ApaI G_GGCC'C Cuts at: 2181 2181 Size: 5797 ApaLI G'TGCA_C Cuts at: 2933 3854 5010 2933 Size: 921 1156 3720 Fragments arranged by size: 3720 1156 921 ApoI r'AATT_y Cuts at: 1493 2276 3106 3332 3484 5593 1493 Size: 783 830 226 152 2109 1697 Fragments arranged by size: 2109 1697 830 783 226 152 AseI AT'TA_AT Cuts at: 160 1032 4351 160 Size: 872 3319 1606 Fragments arranged by size: 3319 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5797 AvaI C'yCGr_G Cuts at: 1870 1943 3122 1870 Size: 73 1179 4545 Fragments arranged by size: 4545 1179 73 BamHI G'GATC_C Cuts at: 3127 3494 3127 Size: 367 5430 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3118 4573 618 Size: 325 217 627 1331 1455 1842 Fragments arranged by size: 1842 1455 1331 627 325 217 BanII G_rGCy'C Cuts at: 1391 2142 2181 3116 1391 Size: 751 39 935 4072 Fragments arranged by size: 4072 935 751 39 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2714 928 Size: 971 815 4011 Fragments arranged by size: 4011 971 815 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3988 4022 1903 Size: 34 2051 34 3678 Fragments arranged by size: 3678 2051 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3689 4899 3689 Size: 1210 4587 BclI T'GATC_A Cuts at: 1548 2559 1548 Size: 1011 4786 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4408 136 Size: 107 122 71 631 605 2736 1525 Fragments arranged by size: 2736 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2503 5792 2503 Size: 3289 2508 BlpI GC'TnA_GC Cuts at: 3214 3214 Size: 5797 Bme1580I G_kGCm'C Cuts at: 1790 2181 2326 2937 3858 5014 1790 Size: 391 145 611 921 1156 2573 Fragments arranged by size: 2573 1156 921 611 391 145 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5797 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2562 3528 4486 Size: 416 53 83 399 24 1145 966 958 Cuts at: 4486 442 Size: 1753 Fragments arranged by size: 1753 1145 966 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5797 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2045 2807 2943 4457 1239 Size: 806 762 136 1514 2579 Fragments arranged by size: 2579 1514 806 762 136 Bpu10I CC'TnA_GC Cuts at: 2034 2250 2034 Size: 216 5581 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2972 3922 4787 5085 2972 Size: 950 865 298 3684 Fragments arranged by size: 3684 950 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4460 882 Size: 617 2961 2219 Fragments arranged by size: 2961 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3588 493 Size: 711 2384 2702 Fragments arranged by size: 2702 2384 711 BsaBI GATnn'nnATC Cuts at: 5791 5791 Size: 5797 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3604 3986 4621 275 Size: 53 83 186 936 2071 382 635 1451 Fragments arranged by size: 2071 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4230 4628 4902 5049 5460 4230 Size: 398 274 147 411 4567 Fragments arranged by size: 4567 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2624 2654 2916 2946 1178 Size: 30 165 30 1221 30 262 30 4029 Fragments arranged by size: 4029 1221 262 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2234 2237 2240 2243 2642 2645 1186 Size: 1048 3 3 3 399 3 4338 Fragments arranged by size: 4338 1048 399 3 3 3 3 BseYI C'CCAG_C Cuts at: 2047 2168 5000 5496 2047 Size: 121 2832 496 2348 Fragments arranged by size: 2832 2348 496 121 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3029 1163 Size: 573 1293 3931 Fragments arranged by size: 3931 1293 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3055 3247 4008 5036 1540 Size: 275 186 1054 192 761 1028 2301 Fragments arranged by size: 2301 1054 1028 761 275 192 186 BsiHKAI G_wGCw'C Cuts at: 2937 3858 3943 5014 2937 Size: 921 85 1071 3720 Fragments arranged by size: 3720 1071 921 85 BsmI GAATG_Cn' Cuts at: 3308 3401 3308 Size: 93 5704 BsmBI CGTCTCn'nnnn_ Cuts at: 3012 3012 Size: 5797 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2142 2181 2326 2937 3116 3858 3943 Size: 399 352 39 145 611 179 742 85 Cuts at: 3943 5014 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 611 399 352 179 145 85 39 BspEI T'CCGG_A Cuts at: 5460 5460 Size: 5797 BspHI T'CATG_A Cuts at: 3686 5416 3686 Size: 1730 4067 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3152 844 Size: 2308 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3684 1394 Size: 429 1861 3507 Fragments arranged by size: 3507 1861 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2530 4292 4466 65 Size: 1183 1282 1762 174 1396 Fragments arranged by size: 1762 1396 1282 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3050 4441 4628 1700 Size: 110 1240 1391 187 2869 Fragments arranged by size: 2869 1391 1240 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5797 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5797 BssSI C'ACGA_G Cuts at: 3857 4869 3857 Size: 1012 4785 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5797 BstBI TT'CG_AA Cuts at: 4601 4601 Size: 5797 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5797 BstXI CCAn_nnnn'nTGG Cuts at: 2150 2150 Size: 5797 BstYI r'GATC_y Cuts at: 1972 2433 2503 2948 3127 3494 3879 3896 5348 Size: 461 70 445 179 367 385 17 1452 Cuts at: 5348 5434 5446 5792 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 461 445 385 367 346 179 86 70 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5245 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5058 Fragments arranged by size: 5058 683 56 BtsI GCAGTG_nn' Cuts at: 2166 3313 4107 4127 2166 Size: 1147 794 20 3836 Fragments arranged by size: 3836 1147 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5797 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5762 DraI TTT'AAA Cuts at: 3101 3101 Size: 5797 DraIII CAC_nnn'GTG Cuts at: 2941 2941 Size: 5797 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4804 809 Size: 507 3488 1802 Fragments arranged by size: 3488 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2643 3052 3244 Size: 54 1425 50 275 18 813 409 192 Cuts at: 3244 4133 5762 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 813 409 275 192 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3052 3244 1537 Size: 275 1240 192 4090 Fragments arranged by size: 4090 1240 275 192 EarI CTCTTCn'nnn_ Cuts at: 1353 2196 2202 2211 3727 1353 Size: 843 6 9 1516 3423 Fragments arranged by size: 3423 1516 843 9 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4384 4758 4904 387 Size: 1064 138 2795 374 146 1280 Fragments arranged by size: 2795 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2045 2703 2807 2943 3860 4457 5244 Size: 393 413 658 104 136 917 597 787 Cuts at: 5244 5512 1239 Size: 268 1524 Fragments arranged by size: 1524 917 787 658 597 413 393 268 136 104 EcoNI CCTnn'n_nnAGG Cuts at: 2789 2789 Size: 5797 EcoRI G'AATT_C Cuts at: 3106 3484 3106 Size: 378 5419 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2059 2059 Size: 5797 FspI TGC'GCA Cuts at: 2549 4303 2549 Size: 1754 4043 HaeII r_GCGC'y Cuts at: 1678 4944 1678 Size: 3266 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2624 2656 2808 2840 4438 Size: 32 135 32 1052 32 152 32 1598 Cuts at: 4438 4470 4512 4544 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1598 1052 152 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2260 3141 3387 669 Size: 988 281 322 881 246 3079 Fragments arranged by size: 3079 988 881 322 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5797 HpaI GTT'AAC Cuts at: 3387 3387 Size: 5797 KpnI G_GTAC'C Cuts at: 3122 3122 Size: 5797 MfeI C'AATT_G Cuts at: 3396 3396 Size: 5797 MluI A'CGCG_T Cuts at: 5673 5673 Size: 5797 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2863 4911 5095 1305 Size: 1558 2048 184 2007 Fragments arranged by size: 2048 2007 1558 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3975 Fragments arranged by size: 3975 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2555 3755 4114 4273 518 Size: 648 1389 1200 359 159 2042 Fragments arranged by size: 2042 1389 1200 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2077 3213 3890 5038 5283 1606 Size: 471 1136 677 1148 245 2120 Fragments arranged by size: 2120 1148 1136 677 471 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5245 NdeI CA'TA_TG Cuts at: 387 387 Size: 5797 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5797 NotI GC'GGCC_GC Cuts at: 3244 3244 Size: 5797 NspI r_CATG'y Cuts at: 1132 1229 3155 4700 1132 Size: 97 1926 1545 2229 Fragments arranged by size: 2229 1926 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5797 PciI A'CATG_T Cuts at: 4696 4696 Size: 5797 PflMI CCAn_nnn'nTGG Cuts at: 1108 3004 1108 Size: 1896 3901 PmeI GTTT'AAAC Cuts at: 3101 3101 Size: 5797 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3866 3898 5379 5411 3866 Size: 32 1481 32 4252 Fragments arranged by size: 4252 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5797 PsiI TTA'TAA Cuts at: 3367 3367 Size: 5797 PspOMI G'GGCC_C Cuts at: 2177 2177 Size: 5797 PstI C_TGCA'G Cuts at: 830 3149 830 Size: 2319 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5797 SalI G'TCGA_C Cuts at: 1936 3139 1936 Size: 1203 4594 SbfI CC_TGCA'GG Cuts at: 3149 3149 Size: 5797 ScaI AGT'ACT Cuts at: 4045 5757 4045 Size: 1712 4085 SexAI A'CCwGG_T Cuts at: 2440 2440 Size: 5797 SfcI C'TryA_G Cuts at: 826 1045 2072 2106 2458 2473 2610 3145 4280 Size: 219 1027 34 352 15 137 535 1135 Cuts at: 4280 4961 5152 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 1027 681 535 352 219 191 137 34 15 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5797 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5797 SmaI CCC'GGG Cuts at: 1872 3124 1872 Size: 1252 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2987 3901 4802 5064 820 Size: 197 926 1044 914 901 262 1553 Fragments arranged by size: 1553 1044 926 914 901 262 197 SnaBI TAC'GTA Cuts at: 493 3588 493 Size: 3095 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5797 SphI G_CATG'C Cuts at: 1132 3155 1132 Size: 2023 3774 SspI AAT'ATT Cuts at: 5 52 3721 5 Size: 47 3669 2081 Fragments arranged by size: 3669 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5797 StyI C'CwwG_G Cuts at: 513 1065 3236 513 Size: 552 2171 3074 Fragments arranged by size: 3074 2171 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3824 3841 3994 4179 4624 325 Size: 1255 2244 17 153 185 445 1498 Fragments arranged by size: 2244 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4043 5755 96 Size: 274 80 33 51 1443 2066 1712 138 Fragments arranged by size: 2066 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2384 2416 2430 2462 2916 2948 5757 5789 2384 Size: 32 14 32 454 32 2809 32 2392 Fragments arranged by size: 2809 2392 454 32 32 32 32 14 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5797 XbaI T'CTAG_A Cuts at: 3085 3133 3085 Size: 48 5749 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5797 XmaI C'CCGG_G Cuts at: 1870 3122 1870 Size: 1252 4545 XmnI GAAnn'nnTTC Cuts at: 3926 3926 Size: 5797 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3605 276 Size: 53 83 186 936 2071 2468 Fragments arranged by size: 2468 2071 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BstZ17I Bsu36I EcoICRI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI