Restriction map for
pFN21AB0441
##################################
# NO CUT ENZYMES ( 107 enzymes) #
##################################
AccI AccII AclI AflII AflIII
AgeI Alw44I AocI ApaLI AscI
AseI AsnI AspHI AspI AviII
BamHI BbeI BbrPI BbsI BbvCI
BcgI BciVI BfrI BglII BlpI
Bpu1102I BpuAI BsaAI BsaBI BsiHKAI
BsiWI BsmBI BsmI BspDI BspLU11I
BspMI BsrBI BsrDI BssHII BssSI
Bst1107I BstBI BstUI BstZ17I Bsu36I
CelII ClaI CpoI Eco81I EcoNI
EcoRI EcoRV EcoT22I FseI FspI
HgaI HindIII HpaI KasI KspI
MamI MfeI MluI MunI MvnI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SacI SacII SalI
SapI ScaI SfiI SfoI SfuI
SgrAI SmlI SnaBI SpeI SphI
SspI SwaI TaqI Tth111I TthHB8I
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 59 enzymes) #
###################################
AatII 1107 GACGTC GACGT^C
Acc65I 227 GGTACC G^GTACC
AccIII 429 TCCGGA T^CCGGA
AcyI 1107 GACGTC G[GA]^CG[CT]C
AlwNI 527 CAGATACTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 1063 AGCGCT AGC^GCT
ApaI 20 GGGCCC GGGCC^C
Asp700I 1218 GAAAGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 227 GGTACC G^GTACC
AvaI 231 CCCGGG C^[CT]CG[GA]G
AvrII 192 CCTAGG C^CTAGG
BalI 878 TGGCCA TGG^CCA
BclI 590 TGATCA T^GATCA
BlnI 192 CCTAGG C^CTAGG
BsaHI 1107 GACGTC G[GA]^CG[CT]C
BsaI 277 GAGACC GGTCTC(1/5)
BsaWI 429 TCCGGA [AT]^CCGG[AT]
BseAI 429 TCCGGA T^CCGGA
BsgI 1264 GTGCAG GTGCAG(16/14)
BsiEI 1259 CGGCCG CG[GA][CT]^CG
BsoBI 231 CCCGGG C^[CT]CG[GA]G
Bsp1407I 380 TGTACA T^GTACA
BspEI 429 TCCGGA T^CCGGA
BspHI 458 TCATGA T^CATGA
BsrFI 125 ACCGGC [GA]^CCGG[CT]
BsrGI 380 TGTACA T^GTACA
BstAPI 367 GCAAACTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 750 GGTGACC G^GT[ACGT]ACC
BstPI 750 GGTGACC G^GT[ACGT]ACC
BstXI 322 CCACAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 125 ACCGGC [GA]^CCGG[CT]
DraI 853 TTTAAA TTT^AAA
DraIII 868 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1195 GACACTGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1259 CGGCCG C^GGCCG
EclXI 1259 CGGCCG C^GGCCG
Eco47III 1063 AGCGCT AGC^GCT
Eco52I 1259 CGGCCG C^GGCCG
EcoO65I 750 GGTGACC G^GT[ACGT]ACC
FbaI 590 TGATCA T^GATCA
HaeII 1063 AGCGCT [GA]GCGC^[CT]
Hin1I 1107 GACGTC G[GA]^CG[CT]C
KpnI 227 GGTACC GGTAC^C
MluNI 878 TGGCCA TGG^CCA
MroI 429 TCCGGA T^CCGGA
MscI 878 TGGCCA TGG^CCA
PleI 843 GACTC GAGTC(4/5)
PpuMI 1093 GGGACCC [GA]G^G[AT]CC[CT]
RcaI 458 TCATGA T^CATGA
SbfI 300 CCTGCAGG CCTGCA^GG
SexAI 869 ACCAGGT A^CC[AT]GGT
SmaI 231 CCCGGG CCC^GGG
Sse8387I 300 CCTGCAGG CCTGCA^GG
SspBI 380 TGTACA T^GTACA
StuI 305 AGGCCT AGG^CCT
TfiI 502 GAATC G^A[AT]TC
XmaCI 231 CCCGGG C^CCGGG
XmaI 231 CCCGGG C^CCGGG
XmnI 1218 GAAAGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AcsI 162 GAATTT [GA]^AATT[CT]
850 GAATTT
AhdI 37 GACCTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
279 GACCCTCTGTC
ApoI 162 GAATTT [GA]^AATT[CT]
850 GAATTT
AspEI 37 GACCTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
279 GACCCTCTGTC
BanI 227 GGTACC G^G[CT][GA]CC
720 GGTGCC
BbvI 608 GCTGC GCAGC(8/12)
782 GCTGC
BfaI 193 CTAG C^TAG
1012 CTAG
BpmI 274 CTGGAG CTGGAG(16/14)
556 CTGGAG
BseRI 559 GAGGAG GAGGAG(10/8)
1279 GAGGAG
BsmAI 277 GAGAC GTCTC(1/5)
1233 GAGAC
BstF5I 30 GGATG GGATG(2/0)
432 GGATG
BstYI 966 GGATCT [GA]^GATC[CT]
1184 AGATCC
CfoI 408 GCGC GCG^C
1064 GCGC
EaeI 878 TGGCCA [CT]^GGCC[GA]
1259 CGGCCG
Eam1105I 37 GACCTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
279 GACCCTCTGTC
EarI 574 CTCTTC CTCTTC(1/4)
1303 CTCTTC
FauI 251 GCGGG CCCGC(4/6)
698 GCGGG
FokI 30 GGATG GGATG(9/13)
432 GGATG
HhaI 408 GCGC GCG^C
1064 GCGC
HinP1I 408 GCGC G^CGC
1064 GCGC
HincII 13 GTCAAC GT[CT]^[GA]AC
337 GTCAAC
HindII 13 GTCAAC GT[CT]^[GA]AC
337 GTCAAC
HinfI 502 GAATC G^A[ACGT]TC
843 GACTC
Ksp632I 574 CTCTTC CTCTTC(1/4)
1303 CTCTTC
MaeI 193 CTAG C^TAG
1012 CTAG
MflI 966 GGATCT [GA]^GATC[CT]
1184 AGATCC
MslI 25 CACAAGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
739 CACTGCCATG
NcoI 402 CCATGG C^CATGG
450 CCATGG
PflMI 526 CCAGATACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
870 CCAGGTGGTGG
PvuII 553 CAGCTG CAG^CTG
606 CAGCTG
TseI 608 GCTGC G^C[AT]GC
782 GCTGC
Tsp509I 163 AATT ^AATT
851 AATT
Van91I 526 CCAGATACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
870 CCAGGTGGTGG
XhoII 966 GGATCT [GA]^GATC[CT]
1184 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BanII BcnI BglI BmyI BsaJI
BsiYI BslI BsmFI Bsp1286I BsrI
BssKI BstNI Cac8I Cfr13I DdeI
DpnII DraII DsaI EcoO109I EcoRII
EcoT14I Fnu4HI HaeIII HapII HpaII
HphI ItaI MaeII MaeIII MboI
MboII MnlI MseI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI Tru9I
Tsp45I TspRI XcmI