(Circular) (Six-base) MAPSORT of: pFN21AB0441.seq Check: 9896 from: 1 to: 6037 pFN21AB0441 6037 bp With 182 enzymes: SgfI * April 15, 2010 09:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3116 3847 278 Size: 53 83 186 936 1580 731 2468 Fragments arranged by size: 2468 1580 936 731 186 83 53 AccI GT'mk_AC Cuts at: 1937 3380 1937 Size: 1443 4594 Acc65I G'GTAC_C Cuts at: 2232 3358 2232 Size: 1126 4911 AclI AA'CG_TT Cuts at: 1563 4164 4537 1563 Size: 2601 373 3063 Fragments arranged by size: 3063 2601 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2855 4100 5484 5752 1632 Size: 1223 1245 1384 268 1917 Fragments arranged by size: 1917 1384 1245 1223 268 AfeI AGC'GCT Cuts at: 3070 3070 Size: 6037 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5840 AflIII A'CryG_T Cuts at: 1203 4936 5913 1203 Size: 3733 977 1327 Fragments arranged by size: 3733 1327 977 AgeI A'CCGG_T Cuts at: 4868 4868 Size: 6037 AhdI GACnn_n'nnGTC Cuts at: 2047 2289 4766 2047 Size: 242 2477 3318 Fragments arranged by size: 3318 2477 242 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6037 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6037 AlwNI CAG_nnn'CTG Cuts at: 2537 3780 5352 2537 Size: 1243 1572 3222 Fragments arranged by size: 3222 1572 1243 ApaI G_GGCC'C Cuts at: 2029 2029 Size: 6037 ApaLI G'TGCA_C Cuts at: 4094 5250 4094 Size: 1156 4881 ApoI r'AATT_y Cuts at: 1493 2167 2855 3346 3572 3724 5833 1493 Size: 674 688 491 226 152 2109 1697 Fragments arranged by size: 2109 1697 688 674 491 226 152 AseI AT'TA_AT Cuts at: 160 1032 4591 160 Size: 872 3559 1606 Fragments arranged by size: 3559 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6037 AvaI C'yCGr_G Cuts at: 1870 1943 2236 3362 1870 Size: 73 293 1126 4545 Fragments arranged by size: 4545 1126 293 73 AvrII C'CTAG_G Cuts at: 2197 2197 Size: 6037 BamHI G'GATC_C Cuts at: 3367 3734 3367 Size: 367 5670 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2232 2725 3358 4813 618 Size: 325 217 627 445 493 633 1455 1842 Fragments arranged by size: 1842 1455 633 627 493 445 325 217 BanII G_rGCy'C Cuts at: 1391 2029 2305 2899 3356 1391 Size: 638 276 594 457 4072 Fragments arranged by size: 4072 638 594 457 276 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5066 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4228 4262 1903 Size: 34 2291 34 3678 Fragments arranged by size: 3678 2291 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3929 5139 3929 Size: 1210 4827 BclI T'GATC_A Cuts at: 1548 2595 1548 Size: 1047 4990 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2309 2796 3272 Size: 107 122 71 631 605 637 487 476 Cuts at: 3272 4648 136 Size: 1376 1525 Fragments arranged by size: 1525 1376 637 631 605 487 476 122 107 71 BglII A'GATC_T Cuts at: 6032 6032 Size: 6037 BlpI GC'TnA_GC Cuts at: 3454 3454 Size: 6037 Bme1580I G_kGCm'C Cuts at: 1790 2029 2730 4098 5254 1790 Size: 239 701 1368 1156 2573 Fragments arranged by size: 2573 1368 1156 701 239 BmgBI CAC'GTC Cuts at: 1318 2493 1318 Size: 1175 4862 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3075 3768 4726 Size: 416 53 83 399 24 1658 693 958 Cuts at: 4726 442 Size: 1753 Fragments arranged by size: 1753 1658 958 693 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6037 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2300 2582 4697 1239 Size: 1061 282 2115 2579 Fragments arranged by size: 2579 2115 1061 282 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4162 5027 5325 4162 Size: 865 298 4874 Fragments arranged by size: 4874 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2276 4700 882 Size: 617 777 2424 2219 Fragments arranged by size: 2424 2219 777 617 BsaAI yAC'GTr Cuts at: 493 1204 3828 493 Size: 711 2624 2702 Fragments arranged by size: 2702 2624 711 BsaBI GATnn'nnATC Cuts at: 6031 6031 Size: 6037 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3113 3844 4226 4861 Size: 53 83 186 936 1580 731 382 635 Cuts at: 4861 275 Size: 1451 Fragments arranged by size: 1580 1451 936 731 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2434 4470 4868 5142 5289 5700 2434 Size: 2036 398 274 147 411 2771 Fragments arranged by size: 2771 2036 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2348 2378 1178 Size: 30 165 30 945 30 4837 Fragments arranged by size: 4837 945 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2579 3299 1186 Size: 1393 720 3924 Fragments arranged by size: 3924 1393 720 BseYI C'CCAG_C Cuts at: 2334 2920 3094 3103 5240 5736 2334 Size: 586 174 9 2137 496 2635 Fragments arranged by size: 2635 2137 586 496 174 9 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3290 1163 Size: 573 1554 3910 Fragments arranged by size: 3910 1554 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3267 3487 4248 5276 1540 Size: 275 186 1266 220 761 1028 2301 Fragments arranged by size: 2301 1266 1028 761 275 220 186 BsiHKAI G_wGCw'C Cuts at: 4098 4183 5254 4098 Size: 85 1071 4881 Fragments arranged by size: 4881 1071 85 BsmI GAATG_Cn' Cuts at: 3548 3641 3548 Size: 93 5944 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2029 2305 2730 2899 3356 4098 4183 Size: 399 239 276 425 169 457 742 85 Cuts at: 4183 5254 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 457 425 399 276 239 169 85 BspEI T'CCGG_A Cuts at: 2434 5700 2434 Size: 3266 2771 BspHI T'CATG_A Cuts at: 2463 3926 5656 2463 Size: 1463 1730 2844 Fragments arranged by size: 2844 1730 1463 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3392 844 Size: 2548 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3924 1394 Size: 429 2101 3507 Fragments arranged by size: 3507 2101 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4532 4706 65 Size: 1183 3284 174 1396 Fragments arranged by size: 3284 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2130 4681 4868 1700 Size: 110 320 2551 187 2869 Fragments arranged by size: 2869 2551 320 187 110 BsrGI T'GTAC_A Cuts at: 96 2385 96 Size: 2289 3748 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6037 BssSI C'ACGA_G Cuts at: 4097 5109 4097 Size: 1012 5025 BstAPI GCAn_nnn'nTGC Cuts at: 794 2378 794 Size: 1584 4453 BstBI TT'CG_AA Cuts at: 4841 4841 Size: 6037 BstEII G'GTnAC_C Cuts at: 1184 2755 1184 Size: 1571 4466 BstXI CCAn_nnnn'nTGG Cuts at: 2334 2334 Size: 6037 BstYI r'GATC_y Cuts at: 1972 2971 3189 3367 3734 4119 4136 5588 5674 Size: 999 218 178 367 385 17 1452 86 Cuts at: 5674 5686 6032 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 999 385 367 346 218 178 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2407 2455 3118 513 Size: 552 1342 48 663 3432 Fragments arranged by size: 3432 1342 663 552 48 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2584 487 Size: 683 56 1358 3940 Fragments arranged by size: 3940 1358 683 56 BtsI GCAGTG_nn' Cuts at: 2388 2743 3553 4347 4367 2388 Size: 355 810 794 20 4058 Fragments arranged by size: 4058 810 794 355 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6037 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6002 DraI TTT'AAA Cuts at: 2860 3341 2860 Size: 481 5556 DraIII CAC_nnn'GTG Cuts at: 2878 2878 Size: 6037 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3206 5044 809 Size: 507 1890 1838 1802 Fragments arranged by size: 1890 1838 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2883 3264 3484 Size: 54 1425 50 275 18 1053 381 220 Cuts at: 3484 4373 6002 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1053 889 381 275 220 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3264 3484 1537 Size: 275 1452 220 4090 Fragments arranged by size: 4090 1452 275 220 EarI CTCTTCn'nnn_ Cuts at: 1353 2585 3314 3967 1353 Size: 1232 729 653 3423 Fragments arranged by size: 3423 1232 729 653 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2699 4624 4998 5144 387 Size: 1064 138 1110 1925 374 146 1280 Fragments arranged by size: 1925 1280 1110 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2300 2582 2855 4100 4697 5484 5752 Size: 393 668 282 273 1245 597 787 268 Cuts at: 5752 1239 Size: 1524 Fragments arranged by size: 1524 1245 787 668 597 393 282 273 268 EcoO109I rG'GnC_Cy Cuts at: 2025 2194 2908 3099 2025 Size: 169 714 191 4963 Fragments arranged by size: 4963 714 191 169 EcoRI G'AATT_C Cuts at: 3346 3724 3346 Size: 378 5659 FspI TGC'GCA Cuts at: 4543 4543 Size: 6037 HaeII r_GCGC'y Cuts at: 1678 3072 5184 1678 Size: 1394 2112 2531 Fragments arranged by size: 2531 2112 1394 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2033 2065 2275 2307 4678 Size: 32 135 32 461 32 210 32 2371 Cuts at: 4678 4710 4752 4784 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2371 461 210 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2020 2344 3381 3627 669 Size: 988 281 82 324 1037 246 3079 Fragments arranged by size: 3079 1037 988 324 281 246 82 HindIII A'AGCT_T Cuts at: 748 748 Size: 6037 HpaI GTT'AAC Cuts at: 3627 3627 Size: 6037 KpnI G_GTAC'C Cuts at: 2236 3362 2236 Size: 1126 4911 MfeI C'AATT_G Cuts at: 3636 3636 Size: 6037 MluI A'CGCG_T Cuts at: 5913 5913 Size: 6037 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2122 5151 5335 1305 Size: 817 3029 184 2007 Fragments arranged by size: 3029 2007 817 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2885 10 Size: 54 1425 343 1053 3162 Fragments arranged by size: 3162 1425 1053 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2034 2748 3995 4354 4513 518 Size: 648 843 25 714 1247 359 159 2042 Fragments arranged by size: 2042 1247 843 714 648 359 159 25 MspA1I CmG'CkG Cuts at: 1606 2560 2613 2703 3453 4130 5278 5523 1606 Size: 954 53 90 750 677 1148 245 2120 Fragments arranged by size: 2120 1148 954 750 677 245 90 53 NcoI C'CATG_G Cuts at: 513 1065 2407 2455 513 Size: 552 1342 48 4095 Fragments arranged by size: 4095 1342 552 48 NdeI CA'TA_TG Cuts at: 387 387 Size: 6037 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6037 NotI GC'GGCC_GC Cuts at: 3484 3484 Size: 6037 NspI r_CATG'y Cuts at: 1132 1229 3395 4940 1132 Size: 97 2166 1545 2229 Fragments arranged by size: 2229 2166 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6037 PciI A'CATG_T Cuts at: 4936 4936 Size: 6037 PflMI CCAn_nnn'nTGG Cuts at: 1108 2537 2881 1108 Size: 1429 344 4264 Fragments arranged by size: 4264 1429 344 PfoI T'CCnGG_A Cuts at: 3279 3279 Size: 6037 PmeI GTTT'AAAC Cuts at: 3341 3341 Size: 6037 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4106 4138 5619 5651 4106 Size: 32 1481 32 4492 Fragments arranged by size: 4492 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3099 3099 Size: 6037 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6037 PsiI TTA'TAA Cuts at: 3607 3607 Size: 6037 PspOMI G'GGCC_C Cuts at: 2025 2025 Size: 6037 PstI C_TGCA'G Cuts at: 830 2310 2894 3189 3389 830 Size: 1480 584 295 200 3478 Fragments arranged by size: 3478 1480 584 295 200 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6037 PvuII CAG'CTG Cuts at: 2560 2613 2560 Size: 53 5984 SalI G'TCGA_C Cuts at: 1936 3379 1936 Size: 1443 4594 SanDI GG'GwC_CC Cuts at: 3099 3099 Size: 6037 SbfI CC_TGCA'GG Cuts at: 2310 3389 2310 Size: 1079 4958 ScaI AGT'ACT Cuts at: 4285 5997 4285 Size: 1712 4325 SexAI A'CCwGG_T Cuts at: 2874 2874 Size: 6037 SfcI C'TryA_G Cuts at: 826 1045 2306 2809 2890 3185 3385 4520 5201 Size: 219 1261 503 81 295 200 1135 681 Cuts at: 5201 5392 826 Size: 191 1471 Fragments arranged by size: 1471 1261 1135 681 503 295 219 200 191 81 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6037 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6037 SmaI CCC'GGG Cuts at: 1872 2238 3364 1872 Size: 366 1126 4545 Fragments arranged by size: 4545 1126 366 SmlI C'TyrA_G Cuts at: 820 1017 1943 4141 5042 5304 820 Size: 197 926 2198 901 262 1553 Fragments arranged by size: 2198 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3828 493 Size: 3335 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6037 SphI G_CATG'C Cuts at: 1132 3395 1132 Size: 2263 3774 SspI AAT'ATT Cuts at: 5 52 3961 5 Size: 47 3909 2081 Fragments arranged by size: 3909 2081 47 StuI AGG'CCT Cuts at: 1517 2312 1517 Size: 795 5242 StyI C'CwwG_G Cuts at: 513 1065 2197 2407 2455 3476 513 Size: 552 1132 210 48 1021 3074 Fragments arranged by size: 3074 1132 1021 552 210 48 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4064 4081 4234 4419 4864 325 Size: 1255 2484 17 153 185 445 1498 Fragments arranged by size: 2484 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2385 4283 5995 Size: 274 80 33 51 1443 408 1898 1712 Cuts at: 5995 96 Size: 138 Fragments arranged by size: 1898 1712 1443 408 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2315 2347 2565 2597 5997 6029 2315 Size: 32 218 32 3400 32 2323 Fragments arranged by size: 3400 2323 218 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6037 XbaI T'CTAG_A Cuts at: 3373 3373 Size: 6037 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2249 2453 3200 2249 Size: 204 747 5086 Fragments arranged by size: 5086 747 204 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6037 XmaI C'CCGG_G Cuts at: 1870 2236 3362 1870 Size: 366 1126 4545 Fragments arranged by size: 4545 1126 366 XmnI GAAnn'nnTTC Cuts at: 3227 4166 3227 Size: 939 5098 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3114 3845 276 Size: 53 83 186 936 1580 731 2468 Fragments arranged by size: 2468 1580 936 731 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SapI SfiI SfoI SrfI SwaI