Restriction map for
pFN21AB0441
(Linear) (Six-base) MAPSORT of: KIBB0441.seq Check: 8560 from: 1 to: 1332
KIBB0441.orf
With 182 enzymes: SgfI *
October 7, 2009 14:13 ..
AatII G_ACGT'C
Cuts at: 0 1111 1332
Size: 1111 221
Acc65I G'GTAC_C
Cuts at: 0 227 1332
Size: 227 1105
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 850 1332
Size: 850 482
AfeI AGC'GCT
Cuts at: 0 1065 1332
Size: 1065 267
AhdI GACnn_n'nnGTC
Cuts at: 0 42 284 1332
Size: 42 242 1048
Fragments arranged by size:
1048 242 42
AlwNI CAG_nnn'CTG
Cuts at: 0 532 1332
Size: 532 800
ApaI G_GGCC'C
Cuts at: 0 24 1332
Size: 24 1308
ApoI r'AATT_y
Cuts at: 0 162 850 1332
Size: 162 688 482
Fragments arranged by size:
688 482 162
AvaI C'yCGr_G
Cuts at: 0 231 1332
Size: 231 1101
AvrII C'CTAG_G
Cuts at: 0 192 1332
Size: 192 1140
BanI G'GyrC_C
Cuts at: 0 227 720 1332
Size: 227 493 612
Fragments arranged by size:
612 493 227
BanII G_rGCy'C
Cuts at: 0 24 300 894 1332
Size: 24 276 594 438
Fragments arranged by size:
594 438 276 24
BclI T'GATC_A
Cuts at: 0 590 1332
Size: 590 742
BglI GCCn_nnn'nGGC
Cuts at: 0 304 791 1267 1332
Size: 304 487 476 65
Fragments arranged by size:
487 476 304 65
Bme1580I G_kGCm'C
Cuts at: 0 24 725 1332
Size: 24 701 607
Fragments arranged by size:
701 607 24
BmgBI CAC'GTC
Cuts at: 0 488 1332
Size: 488 844
BmrI ACTGGGnnnn_n'
Cuts at: 0 1070 1332
Size: 1070 262
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 295 577 1332
Size: 295 282 755
Fragments arranged by size:
755 295 282
BsaI GGTCTCn'nnnn_
Cuts at: 0 271 1332
Size: 271 1061
BsaHI Gr'CG_yC
Cuts at: 0 1108 1332
Size: 1108 224
BsaWI w'CCGG_w
Cuts at: 0 429 1332
Size: 429 903
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 343 373 1332
Size: 343 30 959
Fragments arranged by size:
959 343 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 574 1294 1332
Size: 574 720 38
Fragments arranged by size:
720 574 38
BseYI C'CCAG_C
Cuts at: 0 329 915 1089 1098 1332
Size: 329 586 174 9 234
Fragments arranged by size:
586 329 234 174 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1285 1332
Size: 1285 47
BsiEI CG_ry'CG
Cuts at: 0 1262 1332
Size: 1262 70
Bsp1286I G_dGCh'C
Cuts at: 0 24 300 725 894 1332
Size: 24 276 425 169 438
Fragments arranged by size:
438 425 276 169 24
BspEI T'CCGG_A
Cuts at: 0 429 1332
Size: 429 903
BspHI T'CATG_A
Cuts at: 0 458 1332
Size: 458 874
BsrFI r'CCGG_y
Cuts at: 0 125 1332
Size: 125 1207
BsrGI T'GTAC_A
Cuts at: 0 380 1332
Size: 380 952
BstAPI GCAn_nnn'nTGC
Cuts at: 0 373 1332
Size: 373 959
BstEII G'GTnAC_C
Cuts at: 0 750 1332
Size: 750 582
BstXI CCAn_nnnn'nTGG
Cuts at: 0 329 1332
Size: 329 1003
BstYI r'GATC_y
Cuts at: 0 966 1184 1332
Size: 966 218 148
Fragments arranged by size:
966 218 148
BtgI C'CryG_G
Cuts at: 0 402 450 1113 1332
Size: 402 48 663 219
Fragments arranged by size:
663 402 219 48
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 579 1332
Size: 579 753
BtsI GCAGTG_nn'
Cuts at: 0 383 738 1332
Size: 383 355 594
Fragments arranged by size:
594 383 355
DraI TTT'AAA
Cuts at: 0 855 1332
Size: 855 477
DraIII CAC_nnn'GTG
Cuts at: 0 873 1332
Size: 873 459
DrdI GACnn_nn'nnGTC
Cuts at: 0 1201 1332
Size: 1201 131
EaeI y'GGCC_r
Cuts at: 0 878 1259 1332
Size: 878 381 73
Fragments arranged by size:
878 381 73
EagI C'GGCC_G
Cuts at: 0 1259 1332
Size: 1259 73
EarI CTCTTCn'nnn_
Cuts at: 0 580 1309 1332
Size: 580 729 23
Fragments arranged by size:
729 580 23
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 694 1332
Size: 694 638
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 295 577 850 1332
Size: 295 282 273 482
Fragments arranged by size:
482 295 282 273
EcoO109I rG'GnC_Cy
Cuts at: 0 20 189 903 1094 1332
Size: 20 169 714 191 238
Fragments arranged by size:
714 238 191 169 20
HaeII r_GCGC'y
Cuts at: 0 1067 1332
Size: 1067 265
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 28 60 270 302 1332
Size: 28 32 210 32 1030
Fragments arranged by size:
1030 210 32 32 28
HincII GTy'rAC
Cuts at: 0 15 339 1332
Size: 15 324 993
Fragments arranged by size:
993 324 15
KpnI G_GTAC'C
Cuts at: 0 231 1332
Size: 231 1101
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 117 1332
Size: 117 1215
MscI TGG'CCA
Cuts at: 0 880 1332
Size: 880 452
MslI CAynn'nnrTG
Cuts at: 0 29 743 1332
Size: 29 714 589
Fragments arranged by size:
714 589 29
MspA1I CmG'CkG
Cuts at: 0 555 608 698 1332
Size: 555 53 90 634
Fragments arranged by size:
634 555 90 53
NcoI C'CATG_G
Cuts at: 0 402 450 1332
Size: 402 48 882
Fragments arranged by size:
882 402 48
PflMI CCAn_nnn'nTGG
Cuts at: 0 532 876 1332
Size: 532 344 456
Fragments arranged by size:
532 456 344
PfoI T'CCnGG_A
Cuts at: 0 1274 1332
Size: 1274 58
PpuMI rG'GwC_Cy
Cuts at: 0 1094 1332
Size: 1094 238
PspOMI G'GGCC_C
Cuts at: 0 20 1332
Size: 20 1312
PstI C_TGCA'G
Cuts at: 0 305 889 1184 1332
Size: 305 584 295 148
Fragments arranged by size:
584 305 295 148
PvuII CAG'CTG
Cuts at: 0 555 608 1332
Size: 555 53 724
Fragments arranged by size:
724 555 53
SanDI GG'GwC_CC
Cuts at: 0 1094 1332
Size: 1094 238
SbfI CC_TGCA'GG
Cuts at: 0 305 1332
Size: 305 1027
SexAI A'CCwGG_T
Cuts at: 0 869 1332
Size: 869 463
SfcI C'TryA_G
Cuts at: 0 301 804 885 1180 1332
Size: 301 503 81 295 152
Fragments arranged by size:
503 301 295 152 81
SmaI CCC'GGG
Cuts at: 0 233 1332
Size: 233 1099
StuI AGG'CCT
Cuts at: 0 307 1332
Size: 307 1025
StyI C'CwwG_G
Cuts at: 0 192 402 450 1332
Size: 192 210 48 882
Fragments arranged by size:
882 210 192 48
TatI w'GTAC_w
Cuts at: 0 380 1332
Size: 380 952
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 310 342 560 592 1332
Size: 310 32 218 32 740
Fragments arranged by size:
740 310 218 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 244 448 1195 1332
Size: 244 204 747 137
Fragments arranged by size:
747 244 204 137
XmaI C'CCGG_G
Cuts at: 0 231 1332
Size: 231 1101
XmnI GAAnn'nnTTC
Cuts at: 0 1222 1332
Size: 1222 110
ZraI GAC'GTC
Cuts at: 0 1109 1332
Size: 1109 223
Enzymes that do cut:
AatII Acc65I AcuI AfeI AhdI AlwNI ApaI ApoI
AvaI AvrII BanI BanII BclI BglI Bme1580I BmgBI
BmrI BpmI BsaI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI Bsp1286I BspEI BspHI BsrFI BsrGI BstAPI
BstEII BstXI BstYI BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I HaeII
Hin4I HincII KpnI MmeI MscI MslI MspA1I NcoI
PflMI PfoI PpuMI PspOMI PstI PvuII SanDI SbfI
SexAI SfcI SmaI StuI StyI TatI TstI XcmI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AccI AclI AflII AflIII AgeI AleI AlfI
AloI ApaLI AscI AseI AsiSI BaeI BamHI BbeI
BbsI BbvCI BcgI BciVI BfrBI BglII BlpI BmtI
BplI Bpu10I BpuEI BsaAI BsaBI BsiHKAI BsiWI BsmI
BsmBI BspMI BsrBI BsrDI BssHII BssSI BstBI BstZ17I
Bsu36I ClaI CspCI EcoICRI EcoNI EcoRI EcoRV FalI
FseI FspI FspAI HindIII HpaI KasI MfeI MluI
NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PasI PciI PmeI PmlI PpiI PshAI
PsiI PspXI PsrI PvuI RsrII SacI SacII SalI
SapI ScaI SfiI SfoI SgfI SgrAI SmlI SnaBI
SpeI SphI SrfI SspI SwaI TaqII Tth111I XbaI
XhoI