(Circular) (Six-base) MAPSORT of: pFN21AB0428.seq Check: 7371 from: 1 to: 7261 pFN21AB0428 7261 bp With 182 enzymes: SgfI * April 15, 2010 09:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2726 2726 Size: 7261 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5071 278 Size: 53 83 186 936 3535 2468 Fragments arranged by size: 3535 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4604 1937 Size: 2667 4594 Acc65I G'GTAC_C Cuts at: 4582 4582 Size: 7261 AclI AA'CG_TT Cuts at: 1563 5388 5761 1563 Size: 3825 373 3063 Fragments arranged by size: 3825 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3102 3585 4048 4468 5324 6708 6976 1632 Size: 1470 483 463 420 856 1384 268 1917 Fragments arranged by size: 1917 1470 1384 856 483 463 420 268 AflII C'TTAA_G Cuts at: 820 1017 3907 820 Size: 197 2890 4174 Fragments arranged by size: 4174 2890 197 AflIII A'CryG_T Cuts at: 1203 6160 7137 1203 Size: 4957 977 1327 Fragments arranged by size: 4957 1327 977 AgeI A'CCGG_T Cuts at: 6092 6092 Size: 7261 AhdI GACnn_n'nnGTC Cuts at: 5990 5990 Size: 7261 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7261 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7261 AlwNI CAG_nnn'CTG Cuts at: 2203 2333 2817 3341 3653 5004 6576 2203 Size: 130 484 524 312 1351 1572 2888 Fragments arranged by size: 2888 1572 1351 524 484 312 130 ApaLI G'TGCA_C Cuts at: 2741 4074 4338 4422 5318 6474 2741 Size: 1333 264 84 896 1156 3528 Fragments arranged by size: 3528 1333 1156 896 264 84 ApoI r'AATT_y Cuts at: 1493 3792 4162 4570 4796 4948 7057 1493 Size: 2299 370 408 226 152 2109 1697 Fragments arranged by size: 2299 2109 1697 408 370 226 152 AseI AT'TA_AT Cuts at: 160 1032 5815 160 Size: 872 4783 1606 Fragments arranged by size: 4783 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7261 AvaI C'yCGr_G Cuts at: 1870 1943 4586 1870 Size: 73 2643 4545 Fragments arranged by size: 4545 2643 73 BamHI G'GATC_C Cuts at: 2583 4591 4958 2583 Size: 2008 367 4886 Fragments arranged by size: 4886 2008 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3442 4582 6037 618 Size: 325 217 627 1655 1140 1455 1842 Fragments arranged by size: 1842 1655 1455 1140 627 325 217 BanII G_rGCy'C Cuts at: 1391 2603 4244 4580 1391 Size: 1212 1641 336 4072 Fragments arranged by size: 4072 1641 1212 336 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2314 3853 928 Size: 971 415 1539 4336 Fragments arranged by size: 4336 1539 971 415 BbvCI CC'TCA_GC Cuts at: 3582 3582 Size: 7261 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5452 5486 1903 Size: 34 3515 34 3678 Fragments arranged by size: 3678 3515 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3625 4024 4344 5153 6363 3625 Size: 399 320 809 1210 4523 Fragments arranged by size: 4523 1210 809 399 320 BclI T'GATC_A Cuts at: 1548 1548 Size: 7261 BfrBI ATG'CAT Cuts at: 4006 4006 Size: 7261 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 3063 5872 Size: 107 122 71 631 605 337 1054 2809 Cuts at: 5872 136 Size: 1525 Fragments arranged by size: 2809 1525 1054 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 2315 2590 3779 7256 2315 Size: 275 1189 3477 2320 Fragments arranged by size: 3477 2320 1189 275 BlpI GC'TnA_GC Cuts at: 2853 4678 2853 Size: 1825 5436 Bme1580I G_kGCm'C Cuts at: 1790 2745 4078 4342 4426 5322 6478 1790 Size: 955 1333 264 84 896 1156 2573 Fragments arranged by size: 2573 1333 1156 955 896 264 84 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7261 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2755 3015 3244 Size: 416 53 83 399 24 1338 260 229 Cuts at: 3244 3277 4992 5950 442 Size: 33 1715 958 1753 Fragments arranged by size: 1753 1715 1338 958 416 399 260 229 83 53 33 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7261 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2542 2731 2903 3654 4128 5921 1239 Size: 1303 189 172 751 474 1793 2579 Fragments arranged by size: 2579 1793 1303 751 474 189 172 Bpu10I CC'TnA_GC Cuts at: 2144 2861 3003 3138 3582 2144 Size: 717 142 135 444 5823 Fragments arranged by size: 5823 717 444 142 135 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4228 5386 6251 6549 4228 Size: 1158 865 298 4940 Fragments arranged by size: 4940 1158 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2042 2560 2753 2921 2954 3242 3272 Size: 617 543 518 193 168 33 288 30 Cuts at: 3272 3443 3925 3943 4360 5924 882 Size: 171 482 18 417 1564 2219 Fragments arranged by size: 2219 1564 617 543 518 482 417 288 193 171 168 33 30 18 BsaAI yAC'GTr Cuts at: 493 1204 4421 5052 493 Size: 711 3217 631 2702 Fragments arranged by size: 3217 2702 711 631 BsaBI GATnn'nnATC Cuts at: 7255 7255 Size: 7261 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5068 5450 6085 275 Size: 53 83 186 936 3535 382 635 1451 Fragments arranged by size: 3535 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2546 2880 3300 4092 5694 6092 6366 6513 6924 Size: 334 420 792 1602 398 274 147 411 Cuts at: 6924 2546 Size: 2883 Fragments arranged by size: 2883 1602 792 420 411 398 334 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3652 3682 3876 3906 1178 Size: 30 165 30 2249 30 194 30 4533 Fragments arranged by size: 4533 2249 194 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2279 2762 3128 1186 Size: 1093 483 366 5319 Fragments arranged by size: 5319 1093 483 366 BseYI C'CCAG_C Cuts at: 2093 2424 2596 2691 6464 6960 2093 Size: 331 172 95 3773 496 2394 Fragments arranged by size: 3773 2394 496 331 172 95 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3183 1163 Size: 573 1447 5241 Fragments arranged by size: 5241 1447 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4711 5472 6500 1540 Size: 275 186 2710 761 1028 2301 Fragments arranged by size: 2710 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2745 3004 3670 4078 4244 4342 4426 5322 5407 Size: 259 666 408 166 98 84 896 85 Cuts at: 5407 6478 2745 Size: 1071 3528 Fragments arranged by size: 3528 1071 896 666 408 259 166 98 85 84 BsmI GAATG_Cn' Cuts at: 4772 4865 4772 Size: 93 7168 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2603 2745 3004 3670 4078 4244 4342 Size: 399 813 142 259 666 408 166 98 Cuts at: 4342 4426 4580 5322 5407 6478 1391 Size: 84 154 742 85 1071 2174 Fragments arranged by size: 2174 1071 813 742 666 408 399 259 166 154 142 98 85 84 BspEI T'CCGG_A Cuts at: 2880 3300 6924 2880 Size: 420 3624 3217 Fragments arranged by size: 3624 3217 420 BspHI T'CATG_A Cuts at: 4465 5150 6880 4465 Size: 685 1730 4846 Fragments arranged by size: 4846 1730 685 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2726 2972 4616 844 Size: 1882 246 1644 3489 Fragments arranged by size: 3489 1882 1644 246 BsrBI CCG'CTC Cuts at: 1394 1823 2526 2857 2946 5148 1394 Size: 429 703 331 89 2202 3507 Fragments arranged by size: 3507 2202 703 429 331 89 BsrDI GCAATG_nn' Cuts at: 65 1248 3009 3829 5756 5930 65 Size: 1183 1761 820 1927 174 1396 Fragments arranged by size: 1927 1761 1396 1183 820 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5905 6092 1700 Size: 110 4095 187 2869 Fragments arranged by size: 4095 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3229 96 Size: 3133 4128 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7261 BssSI C'ACGA_G Cuts at: 2279 5321 6333 2279 Size: 3042 1012 3207 Fragments arranged by size: 3207 3042 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2350 794 Size: 1556 5705 BstBI TT'CG_AA Cuts at: 6065 6065 Size: 7261 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7261 BstXI CCAn_nnnn'nTGG Cuts at: 2840 3329 2840 Size: 489 6772 BstYI r'GATC_y Cuts at: 1972 2223 2315 2583 2590 3163 3779 4591 4958 Size: 251 92 268 7 573 616 812 367 Cuts at: 4958 5343 5360 6812 6898 6910 7256 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 812 616 573 385 367 346 268 251 92 86 17 12 7 Bsu36I CC'TnA_GG Cuts at: 3865 3865 Size: 7261 BtgI C'CryG_G Cuts at: 513 1065 2099 2579 3691 513 Size: 552 1034 480 1112 4083 Fragments arranged by size: 4083 1112 1034 552 480 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3084 487 Size: 683 56 1858 4664 Fragments arranged by size: 4664 1858 683 56 BtsI GCAGTG_nn' Cuts at: 2277 3342 4777 5571 5591 2277 Size: 1065 1435 794 20 3947 Fragments arranged by size: 3947 1435 1065 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7261 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7226 DraI TTT'AAA Cuts at: 3400 4565 3400 Size: 1165 6096 DraIII CAC_nnn'GTG Cuts at: 4024 4024 Size: 7261 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6268 809 Size: 507 4952 1802 Fragments arranged by size: 4952 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2010 2947 3436 Size: 54 1425 50 275 18 180 937 489 Cuts at: 3436 3517 4708 5597 7226 8 Size: 81 1191 889 1629 43 Fragments arranged by size: 1629 1425 1191 937 889 489 275 180 81 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4708 1537 Size: 275 2896 4090 Fragments arranged by size: 4090 2896 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2751 2918 2973 3078 3120 3240 3462 3519 Size: 1398 167 55 105 42 120 222 57 Cuts at: 3519 3621 3896 4293 5191 1353 Size: 102 275 397 898 3423 Fragments arranged by size: 3423 1398 898 397 275 222 167 120 105 102 57 55 42 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5848 6222 6368 387 Size: 1064 138 4259 374 146 1280 Fragments arranged by size: 4259 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4242 4242 Size: 7261 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2542 2731 2903 3102 3585 3654 4048 Size: 393 910 189 172 199 483 69 394 Cuts at: 4048 4128 4468 5324 5921 6708 6976 1239 Size: 80 340 856 597 787 268 1524 Fragments arranged by size: 1524 910 856 787 597 483 394 393 340 268 199 189 172 80 69 EcoO109I rG'GnC_Cy Cuts at: 3184 3372 3184 Size: 188 7073 EcoRI G'AATT_C Cuts at: 4570 4948 4570 Size: 378 6883 FspI TGC'GCA Cuts at: 2159 5767 2159 Size: 3608 3653 FspAI rTGC'GCAy Cuts at: 2159 2159 Size: 7261 HaeII r_GCGC'y Cuts at: 1678 6408 1678 Size: 4730 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2307 2339 2575 2607 2986 Size: 32 135 32 735 32 236 32 379 Cuts at: 2986 3018 3176 3208 3824 3856 5902 5934 5976 Size: 32 158 32 616 32 2046 32 42 Cuts at: 5976 6008 1373 Size: 32 2626 Fragments arranged by size: 2626 2046 735 616 379 236 158 135 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3926 4605 4851 669 Size: 988 281 1988 679 246 3079 Fragments arranged by size: 3079 1988 988 679 281 246 HindIII A'AGCT_T Cuts at: 748 3979 4060 4312 4535 748 Size: 3231 81 252 223 3474 Fragments arranged by size: 3474 3231 252 223 81 HpaI GTT'AAC Cuts at: 4851 4851 Size: 7261 KpnI G_GTAC'C Cuts at: 4586 4586 Size: 7261 MfeI C'AATT_G Cuts at: 4860 4860 Size: 7261 MluI A'CGCG_T Cuts at: 7137 7137 Size: 7261 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2080 2544 2879 3368 4049 6375 6559 1305 Size: 775 464 335 489 681 2326 184 2007 Fragments arranged by size: 2326 2007 775 681 489 464 335 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2012 3438 3519 10 Size: 54 1425 343 180 1426 81 3752 Fragments arranged by size: 3752 1426 1425 343 180 81 54 MslI CAynn'nnrTG Cuts at: 518 1166 4039 4335 4470 5219 5578 5737 518 Size: 648 2873 296 135 749 359 159 2042 Fragments arranged by size: 2873 2042 749 648 359 296 159 135 MspA1I CmG'CkG Cuts at: 1606 2101 3197 3325 4677 5354 6502 6747 1606 Size: 495 1096 128 1352 677 1148 245 2120 Fragments arranged by size: 2120 1352 1148 1096 677 495 245 128 NcoI C'CATG_G Cuts at: 513 1065 2579 513 Size: 552 1514 5195 Fragments arranged by size: 5195 1514 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7261 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7261 NotI GC'GGCC_GC Cuts at: 4708 4708 Size: 7261 NsiI A_TGCA'T Cuts at: 4008 4008 Size: 7261 NspI r_CATG'y Cuts at: 1132 1229 2657 4619 6164 1132 Size: 97 1428 1962 1545 2229 Fragments arranged by size: 2229 1962 1545 1428 97 PasI CC'CwG_GG Cuts at: 1348 2430 3647 1348 Size: 1082 1217 4962 Fragments arranged by size: 4962 1217 1082 PciI A'CATG_T Cuts at: 6160 6160 Size: 7261 PflMI CCAn_nnn'nTGG Cuts at: 1108 2089 2258 4024 1108 Size: 981 169 1766 4345 Fragments arranged by size: 4345 1766 981 169 PfoI T'CCnGG_A Cuts at: 3894 3894 Size: 7261 PmeI GTTT'AAAC Cuts at: 4565 4565 Size: 7261 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5330 5362 6843 6875 5330 Size: 32 1481 32 5716 Fragments arranged by size: 5716 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3184 3372 3184 Size: 188 7073 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7261 PsiI TTA'TAA Cuts at: 4122 4831 4122 Size: 709 6552 PstI C_TGCA'G Cuts at: 830 4613 830 Size: 3783 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7261 PvuII CAG'CTG Cuts at: 3197 3325 3197 Size: 128 7133 SacI G_AGCT'C Cuts at: 4244 4244 Size: 7261 SacII CC_GC'GG Cuts at: 2102 2102 Size: 7261 SalI G'TCGA_C Cuts at: 1936 4603 1936 Size: 2667 4594 SapI GCTCTTCn'nnn_ Cuts at: 2918 3078 3896 2918 Size: 160 818 6283 Fragments arranged by size: 6283 818 160 SbfI CC_TGCA'GG Cuts at: 4613 4613 Size: 7261 ScaI AGT'ACT Cuts at: 5509 7221 5509 Size: 1712 5549 SexAI A'CCwGG_T Cuts at: 4020 4020 Size: 7261 SfcI C'TryA_G Cuts at: 826 1045 3835 4609 5744 6425 6616 826 Size: 219 2790 774 1135 681 191 1471 Fragments arranged by size: 2790 1471 1135 774 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7261 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7261 SmaI CCC'GGG Cuts at: 1872 4588 1872 Size: 2716 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3907 4243 5365 6266 6528 820 Size: 197 926 1964 336 1122 901 262 1553 Fragments arranged by size: 1964 1553 1122 926 901 336 262 197 SnaBI TAC'GTA Cuts at: 493 5052 493 Size: 4559 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7261 SphI G_CATG'C Cuts at: 1132 2657 4619 1132 Size: 1525 1962 3774 Fragments arranged by size: 3774 1962 1525 SspI AAT'ATT Cuts at: 5 52 5185 5 Size: 47 5133 2081 Fragments arranged by size: 5133 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7261 StyI C'CwwG_G Cuts at: 513 1065 2163 2579 4557 4700 513 Size: 552 1098 416 1978 143 3074 Fragments arranged by size: 3074 1978 1098 552 416 143 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2311 2683 2967 3523 4187 4449 5288 Size: 1255 731 372 284 556 664 262 839 Cuts at: 5288 5305 5458 5643 6088 325 Size: 17 153 185 445 1498 Fragments arranged by size: 1498 1255 839 731 664 556 445 372 284 262 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3229 3751 5507 Size: 274 80 33 51 1443 1252 522 1756 Cuts at: 5507 7219 96 Size: 1712 138 Fragments arranged by size: 1756 1712 1443 1252 522 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7221 7253 7221 Size: 32 7229 Tth111I GACn'n_nGTC Cuts at: 1316 4546 1316 Size: 3230 4031 XbaI T'CTAG_A Cuts at: 3166 4597 3166 Size: 1431 5830 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2090 3028 3689 2090 Size: 938 661 5662 Fragments arranged by size: 5662 938 661 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7261 XmaI C'CCGG_G Cuts at: 1870 4586 1870 Size: 2716 4545 XmnI GAAnn'nnTTC Cuts at: 4061 4229 4536 5390 4061 Size: 168 307 854 5932 Fragments arranged by size: 5932 854 307 168 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5069 276 Size: 53 83 186 936 3535 2468 Fragments arranged by size: 3535 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstZ17I EcoNI EcoRV FalI FseI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII SanDI SfiI SfoI SrfI SwaI