(Circular) (Six-base) MAPSORT of: pFN21AB0423.seq Check: 4084 from: 1 to: 6127 pFN21AB0423 6127 bp With 182 enzymes: SgfI * April 15, 2010 09:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3937 278 Size: 53 83 186 936 2401 2468 Fragments arranged by size: 2468 2401 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2140 3470 1937 Size: 203 1330 4594 Fragments arranged by size: 4594 1330 203 Acc65I G'GTAC_C Cuts at: 3448 3448 Size: 6127 AclI AA'CG_TT Cuts at: 1563 4254 4627 1563 Size: 2691 373 3063 Fragments arranged by size: 3063 2691 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3011 4190 5574 5842 1632 Size: 1379 1179 1384 268 1917 Fragments arranged by size: 1917 1384 1379 1179 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5930 AflIII A'CryG_T Cuts at: 1203 5026 6003 1203 Size: 3823 977 1327 Fragments arranged by size: 3823 1327 977 AgeI A'CCGG_T Cuts at: 4958 4958 Size: 6127 AhdI GACnn_n'nnGTC Cuts at: 4856 4856 Size: 6127 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6127 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6127 AlwNI CAG_nnn'CTG Cuts at: 2501 3870 5442 2501 Size: 1369 1572 3186 Fragments arranged by size: 3186 1572 1369 ApaLI G'TGCA_C Cuts at: 4184 5340 4184 Size: 1156 4971 ApoI r'AATT_y Cuts at: 1493 2038 3436 3662 3814 5923 1493 Size: 545 1398 226 152 2109 1697 Fragments arranged by size: 2109 1697 1398 545 226 152 AseI AT'TA_AT Cuts at: 160 1032 4681 160 Size: 872 3649 1606 Fragments arranged by size: 3649 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6127 AvaI C'yCGr_G Cuts at: 1870 1943 2304 3452 1870 Size: 73 361 1148 4545 Fragments arranged by size: 4545 1148 361 73 BamHI G'GATC_C Cuts at: 2617 2789 3457 3824 2617 Size: 172 668 367 4920 Fragments arranged by size: 4920 668 367 172 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2202 3448 4903 618 Size: 325 217 627 415 1246 1455 1842 Fragments arranged by size: 1842 1455 1246 627 415 325 217 BanII G_rGCy'C Cuts at: 1391 3249 3446 1391 Size: 1858 197 4072 Fragments arranged by size: 4072 1858 197 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2448 3272 928 Size: 971 549 824 3783 Fragments arranged by size: 3783 971 824 549 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4318 4352 1903 Size: 34 2381 34 3678 Fragments arranged by size: 3678 2381 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4019 5229 4019 Size: 1210 4917 BclI T'GATC_A Cuts at: 1548 1548 Size: 6127 BfrBI ATG'CAT Cuts at: 2756 2756 Size: 6127 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4738 136 Size: 107 122 71 631 605 3066 1525 Fragments arranged by size: 3066 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6122 6122 Size: 6127 BlpI GC'TnA_GC Cuts at: 3544 3544 Size: 6127 Bme1580I G_kGCm'C Cuts at: 1790 4188 5344 1790 Size: 2398 1156 2573 Fragments arranged by size: 2573 2398 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6127 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3858 4816 442 Size: 416 53 83 399 24 2441 958 1753 Fragments arranged by size: 2441 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6127 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2911 2941 3163 2911 Size: 30 222 5875 Fragments arranged by size: 5875 222 30 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2565 2881 4787 1239 Size: 1326 316 1906 2579 Fragments arranged by size: 2579 1906 1326 316 Bpu10I CC'TnA_GC Cuts at: 2724 2724 Size: 6127 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4252 5117 5415 4252 Size: 865 298 4964 Fragments arranged by size: 4964 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4790 882 Size: 617 3291 2219 Fragments arranged by size: 3291 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3918 493 Size: 711 2714 2702 Fragments arranged by size: 2714 2702 711 BsaBI GATnn'nnATC Cuts at: 6121 6121 Size: 6127 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3934 4316 4951 275 Size: 53 83 186 936 2401 382 635 1451 Fragments arranged by size: 2401 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3000 4560 4958 5232 5379 5790 3000 Size: 1560 398 274 147 411 3337 Fragments arranged by size: 3337 1560 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2195 2225 2768 2798 2893 Size: 30 165 30 792 30 543 30 95 Cuts at: 2893 2923 1178 Size: 30 4382 Fragments arranged by size: 4382 792 543 165 95 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2124 2486 2917 1186 Size: 938 362 431 4396 Fragments arranged by size: 4396 938 431 362 BseYI C'CCAG_C Cuts at: 5330 5826 5330 Size: 496 5631 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2886 1163 Size: 573 1150 4404 Fragments arranged by size: 4404 1150 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3577 4338 5366 1540 Size: 275 186 1576 761 1028 2301 Fragments arranged by size: 2301 1576 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2100 2781 3171 3249 4188 4273 5344 2100 Size: 681 390 78 939 85 1071 2883 Fragments arranged by size: 2883 1071 939 681 390 85 78 BsmI GAATG_Cn' Cuts at: 2642 3638 3731 2642 Size: 996 93 5038 Fragments arranged by size: 5038 996 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2100 2781 3171 3249 3446 4188 4273 Size: 399 310 681 390 78 197 742 85 Cuts at: 4273 5344 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 681 399 390 310 197 85 78 BspEI T'CCGG_A Cuts at: 5790 5790 Size: 6127 BspHI T'CATG_A Cuts at: 2311 4016 5746 2311 Size: 1705 1730 2692 Fragments arranged by size: 2692 1730 1705 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3482 844 Size: 2638 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4014 1394 Size: 429 2191 3507 Fragments arranged by size: 3507 2191 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4622 4796 65 Size: 1183 3374 174 1396 Fragments arranged by size: 3374 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4771 4958 1700 Size: 110 2961 187 2869 Fragments arranged by size: 2961 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6127 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6127 BssSI C'ACGA_G Cuts at: 4187 5199 4187 Size: 1012 5115 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6127 BstBI TT'CG_AA Cuts at: 2082 4931 2082 Size: 2849 3278 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6127 BstXI CCAn_nnnn'nTGG Cuts at: 2289 2333 2366 2672 2848 2289 Size: 44 33 306 176 5568 Fragments arranged by size: 5568 306 176 44 33 BstYI r'GATC_y Cuts at: 1972 2617 2657 2789 3457 3824 4209 4226 5678 Size: 645 40 132 668 367 385 17 1452 Cuts at: 5678 5764 5776 6122 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 668 645 385 367 346 132 86 40 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2691 513 Size: 552 939 687 3949 Fragments arranged by size: 3949 939 687 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2339 487 Size: 683 56 1113 4275 Fragments arranged by size: 4275 1113 683 56 BtsI GCAGTG_nn' Cuts at: 3403 3643 4437 4457 3403 Size: 240 794 20 5073 Fragments arranged by size: 5073 794 240 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6127 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6092 DraI TTT'AAA Cuts at: 3431 3431 Size: 6127 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2243 5134 809 Size: 507 927 2891 1802 Fragments arranged by size: 2891 1802 927 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3574 4463 6092 Size: 54 1425 50 275 18 1744 889 1629 Cuts at: 6092 8 Size: 43 Fragments arranged by size: 1744 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3574 1537 Size: 275 1762 4090 Fragments arranged by size: 4090 1762 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2305 2463 2963 4057 1353 Size: 952 158 500 1094 3423 Fragments arranged by size: 3423 1094 952 500 158 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2210 4714 5088 5234 387 Size: 1064 138 621 2504 374 146 1280 Fragments arranged by size: 2504 1280 1064 621 374 146 138 EcoICRI GAG'CTC Cuts at: 3247 3247 Size: 6127 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2565 2881 3011 4190 4787 5574 5842 Size: 393 933 316 130 1179 597 787 268 Cuts at: 5842 1239 Size: 1524 Fragments arranged by size: 1524 1179 933 787 597 393 316 268 130 EcoNI CCTnn'n_nnAGG Cuts at: 3236 3236 Size: 6127 EcoRI G'AATT_C Cuts at: 3436 3814 3436 Size: 378 5749 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3322 3322 Size: 6127 FspI TGC'GCA Cuts at: 4633 4633 Size: 6127 HaeII r_GCGC'y Cuts at: 1678 5274 1678 Size: 3596 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2768 2800 4768 4800 4842 Size: 32 135 32 1196 32 1968 32 42 Cuts at: 4842 4874 1373 Size: 32 2626 Fragments arranged by size: 2626 1968 1196 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2141 2946 3471 3717 669 Size: 988 281 203 805 525 246 3079 Fragments arranged by size: 3079 988 805 525 281 246 203 HindIII A'AGCT_T Cuts at: 748 748 Size: 6127 HpaI GTT'AAC Cuts at: 3717 3717 Size: 6127 KpnI G_GTAC'C Cuts at: 3452 3452 Size: 6127 MfeI C'AATT_G Cuts at: 2913 3726 2913 Size: 813 5314 MluI A'CGCG_T Cuts at: 6003 6003 Size: 6127 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5241 5425 1305 Size: 3936 184 2007 Fragments arranged by size: 3936 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4305 Fragments arranged by size: 4305 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2364 2670 3105 4085 4444 4603 518 Size: 648 1198 306 435 980 359 159 2042 Fragments arranged by size: 2042 1198 980 648 435 359 306 159 MspA1I CmG'CkG Cuts at: 1606 3277 3543 4220 5368 5613 1606 Size: 1671 266 677 1148 245 2120 Fragments arranged by size: 2120 1671 1148 677 266 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4636 Fragments arranged by size: 4636 939 552 NdeI CA'TA_TG Cuts at: 387 2260 387 Size: 1873 4254 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6127 NotI GC'GGCC_GC Cuts at: 3574 3574 Size: 6127 NsiI A_TGCA'T Cuts at: 2758 2758 Size: 6127 NspI r_CATG'y Cuts at: 1132 1229 3485 5030 1132 Size: 97 2256 1545 2229 Fragments arranged by size: 2256 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6127 PciI A'CATG_T Cuts at: 5026 5026 Size: 6127 PflMI CCAn_nnn'nTGG Cuts at: 1108 2501 1108 Size: 1393 4734 PfoI T'CCnGG_A Cuts at: 2381 2381 Size: 6127 PmeI GTTT'AAAC Cuts at: 3431 3431 Size: 6127 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3307 3339 4196 4228 5709 5741 3307 Size: 32 857 32 1481 32 3693 Fragments arranged by size: 3693 1481 857 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6127 PsiI TTA'TAA Cuts at: 3697 3697 Size: 6127 PstI C_TGCA'G Cuts at: 830 2191 3479 830 Size: 1361 1288 3478 Fragments arranged by size: 3478 1361 1288 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6127 PvuII CAG'CTG Cuts at: 3277 3277 Size: 6127 SacI G_AGCT'C Cuts at: 3249 3249 Size: 6127 SalI G'TCGA_C Cuts at: 1936 2139 3469 1936 Size: 203 1330 4594 Fragments arranged by size: 4594 1330 203 SapI GCTCTTCn'nnn_ Cuts at: 2305 2305 Size: 6127 SbfI CC_TGCA'GG Cuts at: 3479 3479 Size: 6127 ScaI AGT'ACT Cuts at: 4375 6087 4375 Size: 1712 4415 SexAI A'CCwGG_T Cuts at: 2715 2715 Size: 6127 SfcI C'TryA_G Cuts at: 826 1045 2187 3123 3475 4610 5291 5482 826 Size: 219 1142 936 352 1135 681 191 1471 Fragments arranged by size: 1471 1142 1135 936 681 352 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6127 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6127 SmaI CCC'GGG Cuts at: 1872 3454 1872 Size: 1582 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4231 5132 5394 820 Size: 197 926 2288 901 262 1553 Fragments arranged by size: 2288 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3918 493 Size: 3425 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6127 SphI G_CATG'C Cuts at: 1132 3485 1132 Size: 2353 3774 SspI AAT'ATT Cuts at: 5 52 4051 5 Size: 47 3999 2081 Fragments arranged by size: 3999 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6127 StyI C'CwwG_G Cuts at: 513 1065 2004 3341 3566 513 Size: 552 939 1337 225 3074 Fragments arranged by size: 3074 1337 939 552 225 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4154 4171 4324 4509 4954 325 Size: 1255 2574 17 153 185 445 1498 Fragments arranged by size: 2574 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4373 6085 96 Size: 274 80 33 51 1443 2396 1712 138 Fragments arranged by size: 2396 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2195 2227 2377 2409 2893 2925 6087 6119 2195 Size: 32 150 32 484 32 3162 32 2203 Fragments arranged by size: 3162 2203 484 150 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6127 XbaI T'CTAG_A Cuts at: 3157 3463 3157 Size: 306 5821 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6127 XmaI C'CCGG_G Cuts at: 1870 3452 1870 Size: 1582 4545 XmnI GAAnn'nnTTC Cuts at: 3305 4256 3305 Size: 951 5176 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3935 276 Size: 53 83 186 936 2401 2468 Fragments arranged by size: 2468 2401 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI XcmI