(Circular) (Six-base) MAPSORT of: pFN21AB0411.seq Check: 4963 from: 1 to: 6106 pFN21AB0411 6106 bp With 182 enzymes: SgfI * April 15, 2010 09:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3916 278 Size: 53 83 186 936 2380 2468 Fragments arranged by size: 2468 2380 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2460 3449 1937 Size: 523 989 4594 Fragments arranged by size: 4594 989 523 Acc65I G'GTAC_C Cuts at: 2212 3427 2212 Size: 1215 4891 AclI AA'CG_TT Cuts at: 1563 4233 4606 1563 Size: 2670 373 3063 Fragments arranged by size: 3063 2670 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4169 5553 5821 1632 Size: 2537 1384 268 1917 Fragments arranged by size: 2537 1917 1384 268 AfeI AGC'GCT Cuts at: 2993 2993 Size: 6106 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5909 AflIII A'CryG_T Cuts at: 1203 5005 5982 1203 Size: 3802 977 1327 Fragments arranged by size: 3802 1327 977 AgeI A'CCGG_T Cuts at: 4937 4937 Size: 6106 AhdI GACnn_n'nnGTC Cuts at: 2151 4835 2151 Size: 2684 3422 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6106 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3088 3251 1184 Size: 1904 163 4039 Fragments arranged by size: 4039 1904 163 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2407 2439 2631 2663 2407 Size: 32 192 32 5850 Fragments arranged by size: 5850 192 32 32 AlwNI CAG_nnn'CTG Cuts at: 3205 3849 5421 3205 Size: 644 1572 3890 Fragments arranged by size: 3890 1572 644 ApaLI G'TGCA_C Cuts at: 3286 4163 5319 3286 Size: 877 1156 4073 Fragments arranged by size: 4073 1156 877 ApoI r'AATT_y Cuts at: 1493 2325 2684 3415 3641 3793 5902 1493 Size: 832 359 731 226 152 2109 1697 Fragments arranged by size: 2109 1697 832 731 359 226 152 AseI AT'TA_AT Cuts at: 160 1032 2054 4660 160 Size: 872 1022 2606 1606 Fragments arranged by size: 2606 1606 1022 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6106 AvaI C'yCGr_G Cuts at: 1870 1943 2439 3431 1870 Size: 73 496 992 4545 Fragments arranged by size: 4545 992 496 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2204 2237 2204 Size: 33 6073 BamHI G'GATC_C Cuts at: 3436 3803 3436 Size: 367 5739 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2212 2444 2972 3427 4882 Size: 325 217 627 425 232 528 455 1455 Cuts at: 4882 618 Size: 1842 Fragments arranged by size: 1842 1455 627 528 455 425 325 232 217 BanII G_rGCy'C Cuts at: 1391 2891 3425 1391 Size: 1500 534 4072 Fragments arranged by size: 4072 1500 534 BbeI G_GCGC'C Cuts at: 2448 2448 Size: 6106 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3063 928 Size: 971 1164 3971 Fragments arranged by size: 3971 1164 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4297 4331 1903 Size: 34 2360 34 3678 Fragments arranged by size: 3678 2360 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3998 5208 3998 Size: 1210 4896 BclI T'GATC_A Cuts at: 1548 1548 Size: 6106 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4717 136 Size: 107 122 71 631 605 3045 1525 Fragments arranged by size: 3045 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6101 6101 Size: 6106 BlpI GC'TnA_GC Cuts at: 3004 3523 3004 Size: 519 5587 Bme1580I G_kGCm'C Cuts at: 1790 2975 3290 4167 5323 1790 Size: 1185 315 877 1156 2573 Fragments arranged by size: 2573 1185 1156 877 315 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6106 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2130 2507 3837 Size: 416 53 83 399 24 713 377 1330 Cuts at: 3837 4795 442 Size: 958 1753 Fragments arranged by size: 1753 1330 958 713 416 399 377 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6106 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2672 2681 3131 4766 1239 Size: 1433 9 450 1635 2579 Fragments arranged by size: 2579 1635 1433 450 9 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2702 3302 4231 5096 5394 2702 Size: 600 929 865 298 3414 Fragments arranged by size: 3414 929 865 600 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4769 882 Size: 617 3270 2219 Fragments arranged by size: 3270 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3897 493 Size: 711 2693 2702 Fragments arranged by size: 2702 2693 711 BsaBI GATnn'nnATC Cuts at: 6100 6100 Size: 6106 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2445 3913 4295 4930 Size: 53 83 186 936 912 1468 382 635 Cuts at: 4930 275 Size: 1451 Fragments arranged by size: 1468 1451 936 912 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4539 4937 5211 5358 5769 4539 Size: 398 274 147 411 4876 Fragments arranged by size: 4876 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2631 2661 1178 Size: 30 165 30 1228 30 4623 Fragments arranged by size: 4623 1228 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6106 BseYI C'CCAG_C Cuts at: 5309 5805 5309 Size: 496 5610 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2926 1163 Size: 573 1190 4343 Fragments arranged by size: 4343 1190 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3556 4317 5345 1540 Size: 275 186 1555 761 1028 2301 Fragments arranged by size: 2301 1555 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2021 3290 4167 4252 5323 2021 Size: 1269 877 85 1071 2804 Fragments arranged by size: 2804 1269 1071 877 85 BsmI GAATG_Cn' Cuts at: 3617 3710 3617 Size: 93 6013 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2021 2891 2975 3290 3425 4167 4252 Size: 399 231 870 84 315 135 742 85 Cuts at: 4252 5323 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 870 742 399 315 231 135 85 84 BspEI T'CCGG_A Cuts at: 5769 5769 Size: 6106 BspHI T'CATG_A Cuts at: 2758 3995 5725 2758 Size: 1237 1730 3139 Fragments arranged by size: 3139 1730 1237 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3461 844 Size: 2617 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3993 1394 Size: 429 2170 3507 Fragments arranged by size: 3507 2170 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3060 3270 4601 4775 65 Size: 1183 1812 210 1331 174 1396 Fragments arranged by size: 1812 1396 1331 1183 210 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4750 4937 1700 Size: 110 2940 187 2869 Fragments arranged by size: 2940 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3024 96 Size: 2928 3178 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6106 BssSI C'ACGA_G Cuts at: 2564 4166 5178 2564 Size: 1602 1012 3492 Fragments arranged by size: 3492 1602 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6106 BstBI TT'CG_AA Cuts at: 4910 4910 Size: 6106 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6106 BstYI r'GATC_y Cuts at: 1972 3436 3803 4188 4205 5657 5743 5755 6101 Size: 1464 367 385 17 1452 86 12 346 Cuts at: 6101 1972 Size: 1977 Fragments arranged by size: 1977 1464 1452 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2461 2461 Size: 6106 Bsu36I CC'TnA_GG Cuts at: 2856 2856 Size: 6106 BtgI C'CryG_G Cuts at: 513 1065 2004 2216 513 Size: 552 939 212 4403 Fragments arranged by size: 4403 939 552 212 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2557 487 Size: 683 56 1331 4036 Fragments arranged by size: 4036 1331 683 56 BtsI GCAGTG_nn' Cuts at: 2283 3622 4416 4436 2283 Size: 1339 794 20 3953 Fragments arranged by size: 3953 1339 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6106 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6071 DraI TTT'AAA Cuts at: 2067 3410 2067 Size: 1343 4763 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5113 809 Size: 507 3797 1802 Fragments arranged by size: 3797 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2203 3553 4442 Size: 54 1425 50 275 18 373 1350 889 Cuts at: 4442 6071 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1350 889 373 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3553 1537 Size: 275 1741 4090 Fragments arranged by size: 4090 1741 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2034 2109 4036 1353 Size: 681 75 1927 3423 Fragments arranged by size: 3423 1927 681 75 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4693 5067 5213 387 Size: 1064 138 3104 374 146 1280 Fragments arranged by size: 3104 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2672 2681 3131 4169 4766 5553 5821 Size: 393 1040 9 450 1038 597 787 268 Cuts at: 5821 1239 Size: 1524 Fragments arranged by size: 1524 1040 1038 787 597 450 393 268 9 EcoO109I rG'GnC_Cy Cuts at: 2860 3163 2860 Size: 303 5803 EcoRI G'AATT_C Cuts at: 3415 3793 3415 Size: 378 5728 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2479 2479 Size: 6106 FspI TGC'GCA Cuts at: 4612 4612 Size: 6106 HaeII r_GCGC'y Cuts at: 1678 2448 2995 3354 5253 1678 Size: 770 547 359 1899 2531 Fragments arranged by size: 2531 1899 770 547 359 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2137 2169 2627 2659 4747 Size: 32 135 32 565 32 458 32 2088 Cuts at: 4747 4779 4821 4853 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2088 565 458 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3450 3696 669 Size: 988 281 1512 246 3079 Fragments arranged by size: 3079 1512 988 281 246 HindIII A'AGCT_T Cuts at: 748 3102 748 Size: 2354 3752 HpaI GTT'AAC Cuts at: 3696 3696 Size: 6106 KasI G'GCGC_C Cuts at: 2444 2444 Size: 6106 KpnI G_GTAC'C Cuts at: 2216 3431 2216 Size: 1215 4891 MfeI C'AATT_G Cuts at: 3705 3705 Size: 6106 MluI A'CGCG_T Cuts at: 5982 5982 Size: 6106 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2304 5220 5404 1305 Size: 999 2916 184 2007 Fragments arranged by size: 2916 2007 999 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2205 10 Size: 54 1425 343 373 3911 Fragments arranged by size: 3911 1425 373 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2067 2298 2960 4064 4423 4582 518 Size: 648 901 231 662 1104 359 159 2042 Fragments arranged by size: 2042 1104 901 662 648 359 231 159 MspA1I CmG'CkG Cuts at: 1606 3522 4199 5347 5592 1606 Size: 1916 677 1148 245 2120 Fragments arranged by size: 2120 1916 1148 677 245 NarI GG'CG_CC Cuts at: 2445 2445 Size: 6106 NcoI C'CATG_G Cuts at: 513 1065 2004 2216 513 Size: 552 939 212 4403 Fragments arranged by size: 4403 939 552 212 NdeI CA'TA_TG Cuts at: 387 387 Size: 6106 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6106 NotI GC'GGCC_GC Cuts at: 3553 3553 Size: 6106 NspI r_CATG'y Cuts at: 1132 1229 2714 2965 3464 5009 1132 Size: 97 1485 251 499 1545 2229 Fragments arranged by size: 2229 1545 1485 499 251 97 PacI TTA_AT'TAA Cuts at: 2058 2058 Size: 6106 PasI CC'CwG_GG Cuts at: 1348 2982 1348 Size: 1634 4472 PciI A'CATG_T Cuts at: 5005 5005 Size: 6106 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6106 PmeI GTTT'AAAC Cuts at: 3410 3410 Size: 6106 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2631 2663 4175 4207 5688 5720 2631 Size: 32 1512 32 1481 32 3017 Fragments arranged by size: 3017 1512 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2860 2860 Size: 6106 PshAI GACnn'nnGTC Cuts at: 1613 3075 1613 Size: 1462 4644 PsiI TTA'TAA Cuts at: 3676 3676 Size: 6106 PstI C_TGCA'G Cuts at: 830 3077 3458 830 Size: 2247 381 3478 Fragments arranged by size: 3478 2247 381 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6106 SalI G'TCGA_C Cuts at: 1936 3448 1936 Size: 1512 4594 SapI GCTCTTCn'nnn_ Cuts at: 2109 2109 Size: 6106 SbfI CC_TGCA'GG Cuts at: 3458 3458 Size: 6106 ScaI AGT'ACT Cuts at: 4354 6066 4354 Size: 1712 4394 SfcI C'TryA_G Cuts at: 826 1045 2935 3073 3454 4589 5270 5461 826 Size: 219 1890 138 381 1135 681 191 1471 Fragments arranged by size: 1890 1471 1135 681 381 219 191 138 SfoI GGC'GCC Cuts at: 2446 2446 Size: 6106 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6106 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6106 SmaI CCC'GGG Cuts at: 1872 3433 1872 Size: 1561 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2717 3317 4210 5111 5373 820 Size: 197 926 774 600 893 901 262 1553 Fragments arranged by size: 1553 926 901 893 774 600 262 197 SnaBI TAC'GTA Cuts at: 493 3897 493 Size: 3404 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6106 SphI G_CATG'C Cuts at: 1132 3464 1132 Size: 2332 3774 SspI AAT'ATT Cuts at: 5 52 2419 4030 5 Size: 47 2367 1611 2081 Fragments arranged by size: 2367 2081 1611 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6106 StyI C'CwwG_G Cuts at: 513 1065 2004 2216 3545 513 Size: 552 939 212 1329 3074 Fragments arranged by size: 3074 1329 939 552 212 SwaI ATTT'AAAT Cuts at: 2067 2067 Size: 6106 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2689 2852 2974 4133 4150 4303 4488 Size: 1255 1109 163 122 1159 17 153 185 Cuts at: 4488 4933 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1159 1109 445 185 163 153 122 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2010 3024 4352 Size: 274 80 33 51 1443 33 1014 1328 Cuts at: 4352 6064 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1328 1014 274 138 80 51 33 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6066 6098 6066 Size: 32 6074 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6106 XbaI T'CTAG_A Cuts at: 3442 3442 Size: 6106 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6106 XmaI C'CCGG_G Cuts at: 1870 3431 1870 Size: 1561 4545 XmnI GAAnn'nnTTC Cuts at: 4235 4235 Size: 6106 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3914 276 Size: 53 83 186 936 2380 2468 Fragments arranged by size: 2468 2380 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PacI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI ApaI AscI AvrII BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI DraIII EcoICRI EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SrfI XcmI