################################## # NO CUT ENZYMES ( 163 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsiHKAI BsiWI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1286I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII ClaI CpoI DraI DraII DraIII DrdI DsaI Eam1105I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HgaI Hin1I HpaI KasI KpnI KspI MaeI MamI MluI MluNI MroI MscI MspA1I MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AccI 205 GTCGAC GT^[AC][GT]AC AciI 25 GCGG CCGC(-3/-1) Aor51HI 326 AGCGCT AGC^GCT BbsI 1030 GTCTTC GAAGAC(2/6) BpuAI 1030 GTCTTC GAAGAC(2/6) BsaJI 1037 CCTTGG C^C[ACGT][ACGT]GG BsgI 343 GTGCAG GTGCAG(16/14) BsiEI 26 CGGCCG CG[GA][CT]^CG Bsp1407I 410 TGTACA T^GTACA BspMI 345 GCAGGT ACCTGC(4/8) BsrFI 119 GCCGGT [GA]^CCGG[CT] BsrGI 410 TGTACA T^GTACA BstNI 643 CCAGG CC^[AT]GG CfoI 327 GCGC GCG^C Cfr10I 119 GCCGGT [GA]^CCGG[CT] Cfr13I 512 GGGCC G^G[ACGT]CC EaeI 26 CGGCCG [CT]^GGCC[GA] EagI 26 CGGCCG C^GGCCG EclXI 26 CGGCCG C^GGCCG Eco47III 326 AGCGCT AGC^GCT Eco52I 26 CGGCCG C^GGCCG EcoRII 643 CCAGG ^CC[AT]GG EcoT14I 1037 CCTTGG C^C[AT][AT]GG HaeII 326 AGCGCT [GA]GCGC^[CT] HhaI 327 GCGC GCG^C HinP1I 327 GCGC G^CGC HincII 205 GTCGAC GT[CT]^[GA]AC HindII 205 GTCGAC GT[CT]^[GA]AC HindIII 238 AAGCTT A^AGCTT HphI 1034 TCACC GGTGA(8/7) MaeII 1028 ACGT A^CGT MaeIII 695 GTGAC ^GT[ACGT]AC MfeI 473 CAATTG C^AATTG MunI 473 CAATTG C^AATTG MvaI 643 CCAGG CC^[AT]GG NlaIII 483 CATG CATG^ NlaIV 348 GGTTCC GG[ACGT]^[ACGT]CC PleI 647 GACTC GAGTC(4/5) SalI 205 GTCGAC G^TCGAC SapI 91 GAAGAGC GCTCTTC(1/4) Sau96I 512 GGGCC G^G[ACGT]CC ScaI 257 AGTACT AGT^ACT SfcI 575 CTGTAG C^T[GA][CT]AG SspBI 410 TGTACA T^GTACA StyI 1037 CCTTGG C^C[AT][AT]GG TfiI 893 GATTC G^A[AT]TC Tsp45I 695 GTGAC ^GT[GC]AC ################################### # TWO CUT ENZYMES ( 13 enzymes) # ################################### AluI 239 AGCT AG^CT 867 AGCT BcnI 9 CCCGG CC^[GC]GG 29 CCGGG BsiYI 446 CCAAGAAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 515 CCTTGCGATGG BslI 446 CCAAGAAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 515 CCTTGCGATGG BstF5I 42 GGATG GGATG(2/0) 1041 GGATG Cac8I 358 GCTCGC GC[ACGT]^[ACGT]GC 703 GCTTGC FokI 42 GGATG GGATG(9/13) 1041 GGATG HinfI 647 GACTC G^A[ACGT]TC 893 GATTC MslI 822 CATTGGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 837 CATTTTGATG NciI 9 CCCGG CC^[GC]GG 29 CCGGG SfaNI 769 GATGC GCATC(5/9) 1042 GATGC TaqI 206 TCGA T^CGA 389 TCGA TthHB8I 206 TCGA T^CGA 389 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 35 enzymes) # ######################################### AcsI AfaI AlwI ApoI Asp700I BbvI BsrI BssKI BstYI DdeI DpnII EarI Fnu4HI HaeIII HapII HpaII ItaI Ksp632I MboI MboII MflI MnlI MseI MspI MwoI NdeII RsaI Sau3AI ScrFI Tru9I TseI Tsp509I TspRI XhoII XmnI