Restriction map for
pFN21AB8564
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# NO CUT ENZYMES ( 81 enzymes) #
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AclI AflII AflIII AhdI Alw44I
ApaLI AscI AseI AsnI Asp700I
AspEI BamHI BbrPI BbvCI BcgI
BclI BfrI BsaAI BsaBI BsiWI
BsmI Bsp1407I BspHI BspLU11I BsrGI
Bst1107I BstBI BstZ17I CpoI DraI
DraIII Eam1105I EcoRI EcoRV EcoT22I
FbaI FseI HincII HindII HpaI
MamI MfeI MluI MseI MunI
NaeI NdeI NgoMIV NotI NruI
NsiI NspV PacI PflMI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SalI SapI
SbfI ScaI SexAI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI Tru9I Van91I XbaI
XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccI 1048 GTCTAC GT^[AC][GT]AC
AccIII 701 TCCGGA T^CCGGA
AcsI 1297 GAATTT [GA]^AATT[CT]
Aor51HI 959 AGCGCT AGC^GCT
ApoI 1297 GAATTT [GA]^AATT[CT]
AspI 1945 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 110 TGCGCA TGC^GCA
AvrII 1282 CCTAGG C^CTAGG
BalI 2168 TGGCCA TGG^CCA
BciVI 642 GTATCC GTATCC(6/5)
BglII 2603 AGATCT A^GATCT
BlnI 1282 CCTAGG C^CTAGG
BseAI 701 TCCGGA T^CCGGA
BsmBI 357 CGTCTC CGTCTC(1/5)
BspDI 427 ATCGAT AT^CGAT
BspEI 701 TCCGGA T^CCGGA
BsrBI 835 GAGCGG CCGCTC(-3/-3)
BsrDI 603 GCAATG GCAATG(2/0)
BssHII 2159 GCGCGC G^CGCGC
BssSI 659 CACGAG CACGAG(-5/-1)
BstEII 784 GGTGACC G^GT[ACGT]ACC
BstPI 784 GGTGACC G^GT[ACGT]ACC
BstYI 2603 AGATCT [GA]^GATC[CT]
ClaI 427 ATCGAT AT^CGAT
DrdI 237 GACTTTCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 2481 CGGCCG C^GGCCG
EclXI 2481 CGGCCG C^GGCCG
Eco47III 959 AGCGCT AGC^GCT
Eco52I 2481 CGGCCG C^GGCCG
EcoNI 39 CCTGGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 784 GGTGACC G^GT[ACGT]ACC
FspI 110 TGCGCA TGC^GCA
HindIII 1583 AAGCTT A^AGCTT
KspI 223 CCGCGG CCGC^GG
MflI 2603 AGATCT [GA]^GATC[CT]
MluNI 2168 TGGCCA TGG^CCA
MroI 701 TCCGGA T^CCGGA
MscI 2168 TGGCCA TGG^CCA
MslI 1966 CACAATGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NheI 915 GCTAGC G^CTAGC
NspI 1978 ACATGC [GA]CATG^[CT]
PaeR7I 1733 CTCGAG C^TCGAG
PflFI 1945 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
SacII 223 CCGCGG CCGC^GG
SfiI 522 GGCCCAGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
TfiI 1198 GAATC G^A[AT]TC
Tth111I 1945 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 1733 CTCGAG C^TCGAG
XhoII 2603 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 14 enzymes) #
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AatII 355 GACGTC GACGT^C
2663 GACGTC
AlwNI 1115 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
1956 CAGCCCCTG
BglI 523 GCCCAGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1789 GCCACACGGGC
BlpI 369 GCTGAGC GC^T[ACGT]AGC
1455 GCTCAGC
Bpu1102I 369 GCTGAGC GC^T[ACGT]AGC
1455 GCTCAGC
BsaI 1153 GAGACC GGTCTC(1/5)
2137 GAGACC
BsiEI 2481 CGGCCG CG[GA][CT]^CG
2583 CGGTCG
CelII 369 GCTGAGC GC^T[ACGT]AGC
1455 GCTCAGC
NcoI 1313 CCATGG C^CATGG
1605 CCATGG
PvuII 7 CAGCTG CAG^CTG
1171 CAGCTG
SacI 1453 GAGCTC GAGCT^C
2633 GAGCTC
SmlI 1733 CTCGAG C^T[CT][GA]AG
2471 CTCAAG
Tsp509I 1298 AATT ^AATT
1840 AATT
XcmI 957 CCAGCGCTGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1220 CCATCCCCATCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
Acc65I AccII AciI AcyI AfaI
AgeI AluI AlwI AocI ApaI
Asp718I AspHI AvaI AvaII BanI
BanII BbeI BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaHI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
Bsu36I Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI Eco81I EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
KpnI Ksp632I MaeI MaeII MaeIII
MboI MboII MnlI MspA1I MspI
MvaI MvnI MwoI NarI NciI
NdeII NlaIII NlaIV PinAI PleI
PpuMI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SmaI
StuI StyI TaqI TseI Tsp45I
TspRI TthHB8I XmaCI XmaI