(Circular) (Six-base) MAPSORT of: pFN21AB8562.seq Check: 4686 from: 1 to: 7438 pFN21AB8562 7438 bp With 182 enzymes: SgfI * April 15, 2010 09:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5248 278 Size: 53 83 186 936 3712 2468 Fragments arranged by size: 3712 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4781 1937 Size: 2844 4594 Acc65I G'GTAC_C Cuts at: 2162 3743 4759 2162 Size: 1581 1016 4841 Fragments arranged by size: 4841 1581 1016 AclI AA'CG_TT Cuts at: 1563 5565 5938 1563 Size: 4002 373 3063 Fragments arranged by size: 4002 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4621 4637 5501 6885 7153 1632 Size: 2989 16 864 1384 268 1917 Fragments arranged by size: 2989 1917 1384 864 268 16 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7241 AflIII A'CryG_T Cuts at: 1203 3672 6337 7314 1203 Size: 2469 2665 977 1327 Fragments arranged by size: 2665 2469 1327 977 AgeI A'CCGG_T Cuts at: 6269 6269 Size: 7438 AhdI GACnn_n'nnGTC Cuts at: 3450 4298 6167 3450 Size: 848 1869 4721 Fragments arranged by size: 4721 1869 848 AleI CACnn'nnGTG Cuts at: 1166 2687 3338 4187 1166 Size: 1521 651 849 4417 Fragments arranged by size: 4417 1521 849 651 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2978 1184 Size: 1794 5644 AlwNI CAG_nnn'CTG Cuts at: 2194 2221 2566 2998 3220 3379 4250 4660 5181 Size: 27 345 432 222 159 871 410 521 Cuts at: 5181 6753 2194 Size: 1572 2879 Fragments arranged by size: 2879 1572 871 521 432 410 345 222 159 27 ApaI G_GGCC'C Cuts at: 2473 3107 3701 2473 Size: 634 594 6210 Fragments arranged by size: 6210 634 594 ApaLI G'TGCA_C Cuts at: 5495 6651 5495 Size: 1156 6282 ApoI r'AATT_y Cuts at: 1493 2649 4747 4973 5125 7234 1493 Size: 1156 2098 226 152 2109 1697 Fragments arranged by size: 2109 2098 1697 1156 226 152 AseI AT'TA_AT Cuts at: 160 1032 5992 160 Size: 872 4960 1606 Fragments arranged by size: 4960 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7438 AvaI C'yCGr_G Cuts at: 1870 1943 2084 2158 3939 4763 1870 Size: 73 141 74 1781 824 4545 Fragments arranged by size: 4545 1781 824 141 74 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2146 2179 4439 4472 2146 Size: 33 2260 33 5112 Fragments arranged by size: 5112 2260 33 33 BamHI G'GATC_C Cuts at: 2551 4768 5135 2551 Size: 2217 367 4854 Fragments arranged by size: 4854 2217 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2162 2782 3010 3165 3682 Size: 325 217 627 375 620 228 155 517 Cuts at: 3682 3743 3904 4759 6214 618 Size: 61 161 855 1455 1842 Fragments arranged by size: 1842 1455 855 627 620 517 375 325 228 217 161 155 61 BanII G_rGCy'C Cuts at: 1391 2473 3107 3373 3701 4103 4338 4757 1391 Size: 1082 634 266 328 402 235 419 4072 Fragments arranged by size: 4072 1082 634 419 402 328 266 235 BbeI G_GCGC'C Cuts at: 3169 3169 Size: 7438 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2037 2076 2335 2436 3662 4018 4496 Size: 971 138 39 259 101 1226 356 478 Cuts at: 4496 928 Size: 3870 Fragments arranged by size: 3870 1226 971 478 356 259 138 101 39 BbvCI CC'TCA_GC Cuts at: 3934 3934 Size: 7438 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3337 3371 5629 5663 1903 Size: 34 1400 34 2258 34 3678 Fragments arranged by size: 3678 2258 1400 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3874 5330 6540 3874 Size: 1456 1210 4772 Fragments arranged by size: 4772 1456 1210 BclI T'GATC_A Cuts at: 1548 3624 3849 1548 Size: 2076 225 5137 Fragments arranged by size: 5137 2076 225 BfrBI ATG'CAT Cuts at: 3203 3203 Size: 7438 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2903 4574 4612 Size: 107 122 71 631 605 1231 1671 38 Cuts at: 4612 6049 136 Size: 1437 1525 Fragments arranged by size: 1671 1525 1437 1231 631 605 122 107 71 38 BglII A'GATC_T Cuts at: 3570 3630 7433 3570 Size: 60 3803 3575 Fragments arranged by size: 3803 3575 60 BlpI GC'TnA_GC Cuts at: 3372 4403 4855 3372 Size: 1031 452 5955 Fragments arranged by size: 5955 1031 452 Bme1580I G_kGCm'C Cuts at: 1790 2473 2627 2637 3107 3687 3701 4473 5499 Size: 683 154 10 470 580 14 772 1026 Cuts at: 5499 6655 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 1026 772 683 580 470 154 14 10 BmgBI CAC'GTC Cuts at: 1318 4398 1318 Size: 3080 4358 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3971 5169 6127 Size: 416 53 83 399 24 2554 1198 958 Cuts at: 6127 442 Size: 1753 Fragments arranged by size: 2554 1753 1198 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7438 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3592 3631 3592 Size: 39 7399 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2072 2276 2338 2412 2517 2525 2609 2735 Size: 833 204 62 74 105 8 84 126 Cuts at: 2735 2783 3055 4064 6098 1239 Size: 48 272 1009 2034 2579 Fragments arranged by size: 2579 2034 1009 833 272 204 126 105 84 74 62 48 8 Bpu10I CC'TnA_GC Cuts at: 3934 4080 3934 Size: 146 7292 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5563 6428 6726 5563 Size: 865 298 6275 Fragments arranged by size: 6275 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2372 3437 6101 882 Size: 617 873 1065 2664 2219 Fragments arranged by size: 2664 2219 1065 873 617 BsaAI yAC'GTr Cuts at: 493 1204 5229 493 Size: 711 4025 2702 Fragments arranged by size: 4025 2702 711 BsaBI GATnn'nnATC Cuts at: 3629 7432 3629 Size: 3803 3635 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3166 5245 5627 6262 Size: 53 83 186 936 1633 2079 382 635 Cuts at: 6262 275 Size: 1451 Fragments arranged by size: 2079 1633 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5871 6269 6543 6690 7101 5871 Size: 398 274 147 411 6208 Fragments arranged by size: 6208 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4640 4670 1178 Size: 30 165 30 3237 30 3946 Fragments arranged by size: 3946 3237 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2024 2057 2114 2273 2471 2572 3710 3922 Size: 838 33 57 159 198 101 1138 212 Cuts at: 3922 4709 1186 Size: 787 3915 Fragments arranged by size: 3915 1138 838 787 212 198 159 101 57 33 BseYI C'CCAG_C Cuts at: 2244 2728 2809 2841 3364 3945 6641 7137 2244 Size: 484 81 32 523 581 2696 496 2545 Fragments arranged by size: 2696 2545 581 523 496 484 81 32 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2912 3038 3234 3362 1163 Size: 573 1176 126 196 128 5239 Fragments arranged by size: 5239 1176 573 196 128 126 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4888 5649 6677 1540 Size: 275 186 2887 761 1028 2301 Fragments arranged by size: 2887 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3735 5499 5584 6655 3735 Size: 1764 85 1071 4518 Fragments arranged by size: 4518 1764 1071 85 BsmI GAATG_Cn' Cuts at: 3203 3348 4949 5042 3203 Size: 145 1601 93 5599 Fragments arranged by size: 5599 1601 145 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2473 2627 2637 3107 3373 3687 3701 Size: 399 683 154 10 470 266 314 14 Cuts at: 3701 3735 4103 4338 4473 4757 5499 5584 6655 Size: 34 368 235 135 284 742 85 1071 Cuts at: 6655 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 683 470 399 368 314 284 266 235 154 135 85 34 14 10 BspEI T'CCGG_A Cuts at: 7101 7101 Size: 7438 BspHI T'CATG_A Cuts at: 5327 7057 5327 Size: 1730 5708 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2379 4128 4793 844 Size: 1535 1749 665 3489 Fragments arranged by size: 3489 1749 1535 665 BsrBI CCG'CTC Cuts at: 1394 1823 4630 5325 1394 Size: 429 2807 695 3507 Fragments arranged by size: 3507 2807 695 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5933 6107 65 Size: 1183 4685 174 1396 Fragments arranged by size: 4685 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2104 2722 2779 3740 4564 6082 6269 Size: 110 294 618 57 961 824 1518 187 Cuts at: 6269 1700 Size: 2869 Fragments arranged by size: 2869 1518 961 824 618 294 187 110 57 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7438 BssHII G'CGCG_C Cuts at: 1925 2240 1925 Size: 315 7123 BssSI C'ACGA_G Cuts at: 5498 6510 5498 Size: 1012 6426 BstAPI GCAn_nnn'nTGC Cuts at: 794 2725 2998 794 Size: 1931 273 5234 Fragments arranged by size: 5234 1931 273 BstBI TT'CG_AA Cuts at: 3034 6242 3034 Size: 3208 4230 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7438 BstXI CCAn_nnnn'nTGG Cuts at: 4330 4735 4330 Size: 405 7033 BstYI r'GATC_y Cuts at: 1972 2436 2551 3570 3630 4260 4768 5135 5520 Size: 464 115 1019 60 630 508 367 385 Cuts at: 5520 5537 6989 7075 7087 7433 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1019 630 508 464 385 367 346 115 86 60 17 12 Bsu36I CC'TnA_GG Cuts at: 2445 2817 3693 2445 Size: 372 876 6190 Fragments arranged by size: 6190 876 372 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 5947 Fragments arranged by size: 5947 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6699 Fragments arranged by size: 6699 683 56 BtsI GCAGTG_nn' Cuts at: 2996 3333 3345 4545 4954 5748 5768 2996 Size: 337 12 1200 409 794 20 4666 Fragments arranged by size: 4666 1200 794 409 337 20 12 ClaI AT'CG_AT Cuts at: 1554 2832 1554 Size: 1278 6160 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3948 3983 4048 4083 529 Size: 35 3384 35 65 35 3884 Fragments arranged by size: 3884 3384 65 35 35 35 DraI TTT'AAA Cuts at: 4742 4742 Size: 7438 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3830 6445 809 Size: 507 2514 2615 1802 Fragments arranged by size: 2615 2514 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2397 2848 3738 Size: 54 1425 50 275 18 567 451 890 Cuts at: 3738 4149 4725 4885 5774 7403 8 Size: 411 576 160 889 1629 43 Fragments arranged by size: 1629 1425 890 889 576 567 451 411 275 160 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4885 1537 Size: 275 3073 4090 Fragments arranged by size: 4090 3073 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2606 3143 3359 5368 1353 Size: 1253 537 216 2009 3423 Fragments arranged by size: 3423 2009 1253 537 216 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3994 6025 6399 6545 387 Size: 1064 138 2405 2031 374 146 1280 Fragments arranged by size: 2405 2031 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2072 2276 2338 2412 2517 2525 2609 Size: 393 440 204 62 74 105 8 84 Cuts at: 2609 2735 2783 3055 4064 4621 4637 5501 6098 Size: 126 48 272 1009 557 16 864 597 Cuts at: 6098 6885 7153 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1009 864 787 597 557 440 393 272 268 204 126 105 84 74 62 48 16 8 EcoO109I rG'GnC_Cy Cuts at: 2280 2470 2509 2813 2821 3085 3103 3145 3217 Size: 190 39 304 8 264 18 42 72 Cuts at: 3217 3401 3697 3698 3832 2280 Size: 184 296 1 134 5886 Fragments arranged by size: 5886 304 296 264 190 184 134 72 42 39 18 8 1 EcoRI G'AATT_C Cuts at: 2649 4747 5125 2649 Size: 2098 378 4962 Fragments arranged by size: 4962 2098 378 EcoRV GAT'ATC Cuts at: 3160 3160 Size: 7438 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4702 4702 Size: 7438 FspI TGC'GCA Cuts at: 3770 5944 3770 Size: 2174 5264 HaeII r_GCGC'y Cuts at: 1678 3043 3169 6585 1678 Size: 1365 126 3416 2531 Fragments arranged by size: 3416 2531 1365 126 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2428 2460 2969 3001 3436 Size: 32 135 32 856 32 509 32 435 Cuts at: 3436 3468 4284 4316 6079 6111 6153 6185 1373 Size: 32 816 32 1763 32 42 32 2626 Fragments arranged by size: 2626 1763 856 816 509 435 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4782 5028 669 Size: 988 281 2844 246 3079 Fragments arranged by size: 3079 2844 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7438 HpaI GTT'AAC Cuts at: 5028 5028 Size: 7438 KasI G'GCGC_C Cuts at: 3165 3165 Size: 7438 KpnI G_GTAC'C Cuts at: 2166 3747 4763 2166 Size: 1581 1016 4841 Fragments arranged by size: 4841 1581 1016 MfeI C'AATT_G Cuts at: 5037 5037 Size: 7438 MluI A'CGCG_T Cuts at: 7314 7314 Size: 7438 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3297 3951 6552 6736 1305 Size: 1992 654 2601 184 2007 Fragments arranged by size: 2601 2007 1992 654 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2399 4727 10 Size: 54 1425 343 567 2328 2721 Fragments arranged by size: 2721 2328 1425 567 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2687 2867 3338 3989 4187 4223 4328 Size: 648 1521 180 471 651 198 36 105 Cuts at: 4328 4464 4733 5396 5755 5914 518 Size: 136 269 663 359 159 2042 Fragments arranged by size: 2042 1521 663 651 648 471 359 269 198 180 159 136 105 36 MspA1I CmG'CkG Cuts at: 1606 2756 4282 4407 4609 4854 5531 6679 6924 Size: 1150 1526 125 202 245 677 1148 245 Cuts at: 6924 1606 Size: 2120 Fragments arranged by size: 2120 1526 1150 1148 677 245 245 202 125 NaeI GCC'GGC Cuts at: 2106 4566 2106 Size: 2460 4978 NarI GG'CG_CC Cuts at: 3166 3166 Size: 7438 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5947 Fragments arranged by size: 5947 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7438 NgoMIV G'CCGG_C Cuts at: 2104 4564 2104 Size: 2460 4978 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7438 NotI GC'GGCC_GC Cuts at: 4885 4885 Size: 7438 NsiI A_TGCA'T Cuts at: 3205 3205 Size: 7438 NspI r_CATG'y Cuts at: 1132 1229 4504 4796 6341 1132 Size: 97 3275 292 1545 2229 Fragments arranged by size: 3275 2229 1545 292 97 PasI CC'CwG_GG Cuts at: 1348 2512 4244 1348 Size: 1164 1732 4542 Fragments arranged by size: 4542 1732 1164 PciI A'CATG_T Cuts at: 6337 6337 Size: 7438 PflMI CCAn_nnn'nTGG Cuts at: 1108 3220 3961 1108 Size: 2112 741 4585 Fragments arranged by size: 4585 2112 741 PfoI T'CCnGG_A Cuts at: 2712 2946 3636 4041 4263 2712 Size: 234 690 405 222 5887 Fragments arranged by size: 5887 690 405 234 222 PmeI GTTT'AAAC Cuts at: 4742 4742 Size: 7438 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2867 2899 5507 5539 7020 7052 2867 Size: 32 2608 32 1481 32 3253 Fragments arranged by size: 3253 2608 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2813 3217 3401 3832 2813 Size: 404 184 431 6419 Fragments arranged by size: 6419 431 404 184 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7438 PsiI TTA'TAA Cuts at: 3663 5008 3663 Size: 1345 6093 PspOMI G'GGCC_C Cuts at: 2469 3103 3697 2469 Size: 634 594 6210 Fragments arranged by size: 6210 634 594 PstI C_TGCA'G Cuts at: 830 2019 2190 2533 2748 2957 3123 3340 4255 Size: 1189 171 343 215 209 166 217 915 Cuts at: 4255 4790 830 Size: 535 3478 Fragments arranged by size: 3478 1189 915 535 343 217 215 209 171 166 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7438 SalI G'TCGA_C Cuts at: 1936 4780 1936 Size: 2844 4594 SanDI GG'GwC_CC Cuts at: 3832 3832 Size: 7438 SapI GCTCTTCn'nnn_ Cuts at: 2606 3143 2606 Size: 537 6901 SbfI CC_TGCA'GG Cuts at: 4790 4790 Size: 7438 ScaI AGT'ACT Cuts at: 5686 7398 5686 Size: 1712 5726 SfcI C'TryA_G Cuts at: 826 1045 2015 2186 2529 2744 2953 3119 3336 Size: 219 970 171 343 215 209 166 217 Cuts at: 3336 3650 4251 4786 5921 6602 6793 826 Size: 314 601 535 1135 681 191 1471 Fragments arranged by size: 1471 1135 970 681 601 535 343 314 219 217 215 209 191 171 166 SfiI GGCCn_nnn'nGGCC Cuts at: 4574 4574 Size: 7438 SfoI GGC'GCC Cuts at: 3167 3167 Size: 7438 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7438 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7438 SmaI CCC'GGG Cuts at: 1872 2160 3941 4765 1872 Size: 288 1781 824 4545 Fragments arranged by size: 4545 1781 824 288 SmlI C'TyrA_G Cuts at: 820 1017 1943 5542 6443 6705 820 Size: 197 926 3599 901 262 1553 Fragments arranged by size: 3599 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5229 493 Size: 4736 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7438 SphI G_CATG'C Cuts at: 1132 4504 4796 1132 Size: 3372 292 3774 Fragments arranged by size: 3774 3372 292 SspI AAT'ATT Cuts at: 5 52 5362 5 Size: 47 5310 2081 Fragments arranged by size: 5310 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7438 StyI C'CwwG_G Cuts at: 513 1065 2004 2349 4677 4877 513 Size: 552 939 345 2328 200 3074 Fragments arranged by size: 3074 2328 939 552 345 200 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2236 2466 2855 3660 3905 3972 4130 Size: 1255 656 230 389 805 245 67 158 Cuts at: 4130 4217 4465 4476 4537 5465 5482 5635 5820 Size: 87 248 11 61 928 17 153 185 Cuts at: 5820 6265 325 Size: 445 1498 Fragments arranged by size: 1498 1255 928 805 656 445 389 248 245 230 185 158 153 87 67 61 17 11 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3459 4363 5684 Size: 274 80 33 51 1443 1482 904 1321 Cuts at: 5684 7396 96 Size: 1712 138 Fragments arranged by size: 1712 1482 1443 1321 904 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4640 4672 7398 7430 4640 Size: 32 2726 32 4648 Fragments arranged by size: 4648 2726 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7438 XbaI T'CTAG_A Cuts at: 3454 4774 3454 Size: 1320 6118 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3850 3850 Size: 7438 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7438 XmaI C'CCGG_G Cuts at: 1870 2158 3939 4763 1870 Size: 288 1781 824 4545 Fragments arranged by size: 4545 1781 824 288 XmnI GAAnn'nnTTC Cuts at: 2661 5567 2661 Size: 2906 4532 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5246 276 Size: 53 83 186 936 3712 2468 Fragments arranged by size: 3712 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI FseI FspAI NruI PacI PmlI PspXI PsrI PvuII RsrII SacI SacII SexAI SrfI SwaI