Restriction map for
pFN21AB6440
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# NO CUT ENZYMES ( 161 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AflIII
AgeI AhdI AlwI AlwNI AocI
Aor51HI ApaI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BalI BamHI BanI BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BfaI BfrI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaBI BsaHI
BsaI BsaWI BseAI BsiEI BsmAI
BsmBI BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrFI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstYI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraII DsaI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO109I
EcoO65I EcoRI EcoRV FauI FbaI
FseI FspI HgaI Hin1I HincII
HindII HindIII HpaI KasI KpnI
KspI MaeI MamI MfeI MflI
MluI MluNI MroI MscI MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NspV
PacI PflFI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SpeI SphI
Sse8387I SspI StuI SwaI Tsp45I
Tth111I XbaI XcmI XhoII XmaCI
XmaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AcsI 853 AAATTT [GA]^AATT[CT]
Alw44I 369 GTGCAC G^TGCAC
ApaLI 369 GTGCAC G^TGCAC
ApoI 853 AAATTT [GA]^AATT[CT]
Asp700I 482 GAAACAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanII 390 GAGCCC G[GA]GC[CT]^C
BbvI 698 GCTGC GCAGC(8/12)
BpmI 8 CTCCAG CTGGAG(16/14)
BsaAI 141 TACGTA [CT]AC^GT[GA]
BseRI 173 GAGGAG GAGGAG(10/8)
BsgI 561 CTGCAC GTGCAG(16/14)
BsiWI 282 CGTACG C^GTACG
Bsp1407I 235 TGTACA T^GTACA
BspMI 559 ACCTGC ACCTGC(4/8)
BsrGI 235 TGTACA T^GTACA
BstNI 127 CCAGG CC^[AT]GG
Cac8I 152 GCAAGC GC[ACGT]^[ACGT]GC
CfoI 353 GCGC GCG^C
DpnII 315 GATC ^GATC
DraIII 455 CACTTCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 59 GACGGACCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 133 GAAGAG CTCTTC(1/4)
EcoNI 199 CCTCTCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRII 127 CCAGG ^CC[AT]GG
EcoT14I 825 CCAAGG C^C[AT][AT]GG
EcoT22I 1013 ATGCAT ATGCA^T
Fnu4HI 698 GCTGC GC^[ACGT]GC
HaeII 352 GGCGCT [GA]GCGC^[CT]
HhaI 353 GCGC GCG^C
HinP1I 353 GCGC G^CGC
HphI 877 GGTGA GGTGA(8/7)
ItaI 698 GCTGC GC^[ACGT]GC
Ksp632I 133 GAAGAG CTCTTC(1/4)
MaeIII 329 GTAAC ^GT[ACGT]AC
MboI 315 GATC ^GATC
MslI 813 CACTGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 476 CAGCTG C[AC]G^C[GT]G
MvaI 127 CCAGG CC^[AT]GG
NdeII 315 GATC ^GATC
NsiI 1013 ATGCAT ATGCA^T
NspI 531 ACATGC [GA]CATG^[CT]
PaeR7I 27 CTCGAG C^TCGAG
PvuII 476 CAGCTG CAG^CTG
Sau3AI 315 GATC ^GATC
SmlI 27 CTCGAG C^T[CT][GA]AG
SnaBI 141 TACGTA TAC^GTA
SspBI 235 TGTACA T^GTACA
StyI 825 CCAAGG C^C[AT][AT]GG
TseI 698 GCTGC G^C[AT]GC
XhoI 27 CTCGAG C^TCGAG
XmnI 482 GAAACAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 14 enzymes) #
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AvaI 27 CTCGAG C^[CT]CG[GA]G
170 CCCGAG
AvaII 62 GGACC G^G[AT]CC
102 GGTCC
BsaJI 171 CCGAGG C^C[ACGT][ACGT]GG
825 CCAAGG
BsmI 595 GAATGC GAATGC(1/-1)
866 GAATGC
BsoBI 27 CTCGAG C^[CT]CG[GA]G
170 CCCGAG
HaeIII 215 GGCC GG^CC
724 GGCC
MaeII 142 ACGT A^CGT
445 ACGT
MseI 109 TTAA T^TAA
857 TTAA
MwoI 152 GCAAGCCGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
355 GCTGATTCTGC
PflMI 472 CCATCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
631 CCAGACAATGG
TaqI 28 TCGA T^CGA
959 TCGA
Tru9I 109 TTAA T^TAA
857 TTAA
TthHB8I 28 TCGA T^CGA
959 TCGA
Van91I 472 CCATCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
631 CCAGACAATGG
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# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AfaI AluI AspHI BcnI BmyI
BsiHKAI BsiYI BslI BsmFI Bsp1286I
BsrI BssKI BstF5I Cfr13I DdeI
FokI HapII HinfI HpaII MboII
MnlI MspI NciI NlaIII NlaIV
RsaI Sau96I ScrFI SfaNI TfiI
Tsp509I TspRI