(Circular) (Six-base) MAPSORT of: pFN21AB8545.seq Check: 4296 from: 1 to: 7657 pFN21AB8545 7657 bp With 182 enzymes: SgfI * April 15, 2010 09:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5467 278 Size: 53 83 186 936 3931 2468 Fragments arranged by size: 3931 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2250 3850 4101 5000 1937 Size: 313 1600 251 899 4594 Fragments arranged by size: 4594 1600 899 313 251 Acc65I G'GTAC_C Cuts at: 4978 4978 Size: 7657 AclI AA'CG_TT Cuts at: 1563 5784 6157 1563 Size: 4221 373 3063 Fragments arranged by size: 4221 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2573 3589 5720 7104 7372 1632 Size: 941 1016 2131 1384 268 1917 Fragments arranged by size: 2131 1917 1384 1016 941 268 AfeI AGC'GCT Cuts at: 4771 4771 Size: 7657 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7460 AflIII A'CryG_T Cuts at: 1203 6556 7533 1203 Size: 5353 977 1327 Fragments arranged by size: 5353 1327 977 AgeI A'CCGG_T Cuts at: 6488 6488 Size: 7657 AhdI GACnn_n'nnGTC Cuts at: 6386 6386 Size: 7657 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7657 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7657 AlwNI CAG_nnn'CTG Cuts at: 2539 3818 5400 6972 2539 Size: 1279 1582 1572 3224 Fragments arranged by size: 3224 1582 1572 1279 ApaLI G'TGCA_C Cuts at: 5714 6870 5714 Size: 1156 6501 ApoI r'AATT_y Cuts at: 1493 3300 3344 3486 4125 4469 4850 4966 5192 Size: 1807 44 142 639 344 381 116 226 Cuts at: 5192 5344 7453 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1807 1697 639 381 344 226 152 142 116 44 AseI AT'TA_AT Cuts at: 160 1032 3540 6211 160 Size: 872 2508 2671 1606 Fragments arranged by size: 2671 2508 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7657 AvaI C'yCGr_G Cuts at: 1870 1943 4089 4884 4982 1870 Size: 73 2146 795 98 4545 Fragments arranged by size: 4545 2146 795 98 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2109 2142 2471 2504 2109 Size: 33 329 33 7262 Fragments arranged by size: 7262 329 33 33 BamHI G'GATC_C Cuts at: 4987 5354 4987 Size: 367 7290 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3599 4698 4978 6433 618 Size: 325 217 627 1812 1099 280 1455 1842 Fragments arranged by size: 1842 1812 1455 1099 627 325 280 217 BanII G_rGCy'C Cuts at: 1391 3526 4976 1391 Size: 2135 1450 4072 Fragments arranged by size: 4072 2135 1450 BbeI G_GCGC'C Cuts at: 4702 4702 Size: 7657 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2060 3466 928 Size: 971 161 1406 5119 Fragments arranged by size: 5119 1406 971 161 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4703 4737 5848 5882 1903 Size: 34 2766 34 1111 34 3678 Fragments arranged by size: 3678 2766 1111 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4641 4890 5549 6759 4641 Size: 249 659 1210 5539 Fragments arranged by size: 5539 1210 659 249 BclI T'GATC_A Cuts at: 1548 1548 Size: 7657 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6268 136 Size: 107 122 71 631 605 4596 1525 Fragments arranged by size: 4596 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3938 7652 3938 Size: 3714 3943 BlpI GC'TnA_GC Cuts at: 2532 5074 2532 Size: 2542 5115 Bme1580I G_kGCm'C Cuts at: 1790 5718 6874 1790 Size: 3928 1156 2573 Fragments arranged by size: 3928 2573 1156 BmgBI CAC'GTC Cuts at: 1318 2927 1318 Size: 1609 6048 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5388 6346 442 Size: 416 53 83 399 24 3971 958 1753 Fragments arranged by size: 3971 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3681 4713 1056 Size: 2625 1032 4000 Fragments arranged by size: 4000 2625 1032 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2352 2528 3657 3714 6317 1239 Size: 1113 176 1129 57 2603 2579 Fragments arranged by size: 2603 2579 1129 1113 176 57 Bpu10I CC'TnA_GC Cuts at: 2737 2737 Size: 7657 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2760 3597 3734 3947 5782 6647 6945 2760 Size: 837 137 213 1835 865 298 3472 Fragments arranged by size: 3472 1835 865 837 298 213 137 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3333 6320 882 Size: 617 1834 2987 2219 Fragments arranged by size: 2987 2219 1834 617 BsaAI yAC'GTr Cuts at: 493 1204 3290 5448 493 Size: 711 2086 2158 2702 Fragments arranged by size: 2702 2158 2086 711 BsaBI GATnn'nnATC Cuts at: 7651 7651 Size: 7657 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2009 4699 5464 5846 Size: 53 83 186 936 476 2690 765 382 Cuts at: 5846 6481 275 Size: 635 1451 Fragments arranged by size: 2690 1451 936 765 635 476 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6090 6488 6762 6909 7320 6090 Size: 398 274 147 411 6427 Fragments arranged by size: 6427 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3073 3103 4068 4098 1178 Size: 30 165 30 1670 30 965 30 4737 Fragments arranged by size: 4737 1670 965 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3516 3717 3879 4925 1186 Size: 2330 201 162 1046 3918 Fragments arranged by size: 3918 2330 1046 201 162 BseYI C'CCAG_C Cuts at: 6860 7356 6860 Size: 496 7161 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2147 2487 1163 Size: 573 411 340 6333 Fragments arranged by size: 6333 573 411 340 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5107 5868 6896 1540 Size: 275 186 3106 761 1028 2301 Fragments arranged by size: 3106 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3526 5718 5803 6874 3526 Size: 2192 85 1071 4309 Fragments arranged by size: 4309 2192 1071 85 BsmI GAATG_Cn' Cuts at: 2583 5168 5261 2583 Size: 2585 93 4979 Fragments arranged by size: 4979 2585 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3526 4976 5718 5803 6874 1391 Size: 399 1736 1450 742 85 1071 2174 Fragments arranged by size: 2174 1736 1450 1071 742 399 85 BspEI T'CCGG_A Cuts at: 7320 7320 Size: 7657 BspHI T'CATG_A Cuts at: 3108 5546 7276 3108 Size: 2438 1730 3489 Fragments arranged by size: 3489 2438 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 5012 844 Size: 4168 3489 BsrBI CCG'CTC Cuts at: 1394 1823 5544 1394 Size: 429 3721 3507 Fragments arranged by size: 3721 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4941 6152 6326 65 Size: 1183 3693 1211 174 1396 Fragments arranged by size: 3693 1396 1211 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4226 6301 6488 1700 Size: 110 2416 2075 187 2869 Fragments arranged by size: 2869 2416 2075 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7657 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7657 BssSI C'ACGA_G Cuts at: 3741 5717 6729 3741 Size: 1976 1012 4669 Fragments arranged by size: 4669 1976 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2708 794 Size: 1914 5743 BstBI TT'CG_AA Cuts at: 6461 6461 Size: 7657 BstEII G'GTnAC_C Cuts at: 1184 3430 1184 Size: 2246 5411 BstXI CCAn_nnnn'nTGG Cuts at: 3383 3383 Size: 7657 BstYI r'GATC_y Cuts at: 1972 2335 2498 3938 4987 5354 5739 5756 7208 Size: 363 163 1440 1049 367 385 17 1452 Cuts at: 7208 7294 7306 7652 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1440 1049 385 367 363 346 163 86 17 12 BstZ17I GTA'TAC Cuts at: 3851 3851 Size: 7657 BtgI C'CryG_G Cuts at: 513 1065 2004 2521 513 Size: 552 939 517 5649 Fragments arranged by size: 5649 939 552 517 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6918 Fragments arranged by size: 6918 683 56 BtsI GCAGTG_nn' Cuts at: 3068 5173 5967 5987 3068 Size: 2105 794 20 4738 Fragments arranged by size: 4738 2105 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7657 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7622 DraI TTT'AAA Cuts at: 2817 4961 2817 Size: 2144 5513 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6664 809 Size: 507 5348 1802 Fragments arranged by size: 5348 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3217 5104 5993 Size: 54 1425 50 275 18 1387 1887 889 Cuts at: 5993 7622 8 Size: 1629 43 Fragments arranged by size: 1887 1629 1425 1387 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5104 1537 Size: 275 3292 4090 Fragments arranged by size: 4090 3292 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3565 3991 4584 4902 5587 1353 Size: 2212 426 593 318 685 3423 Fragments arranged by size: 3423 2212 685 593 426 318 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6244 6618 6764 387 Size: 1064 138 4655 374 146 1280 Fragments arranged by size: 4655 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3524 3524 Size: 7657 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2352 2528 2573 3589 3657 3714 5720 Size: 393 720 176 45 1016 68 57 2006 Cuts at: 5720 6317 7104 7372 1239 Size: 597 787 268 1524 Fragments arranged by size: 2006 1524 1016 787 720 597 393 268 176 68 57 45 EcoNI CCTnn'n_nnAGG Cuts at: 3894 3894 Size: 7657 EcoO109I rG'GnC_Cy Cuts at: 2422 4234 4528 2422 Size: 1812 294 5551 Fragments arranged by size: 5551 1812 294 EcoRI G'AATT_C Cuts at: 4966 5344 4966 Size: 378 7279 EcoRV GAT'ATC Cuts at: 2146 2940 3025 2146 Size: 794 85 6778 Fragments arranged by size: 6778 794 85 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2259 3023 2259 Size: 764 6893 FspI TGC'GCA Cuts at: 6163 6163 Size: 7657 HaeII r_GCGC'y Cuts at: 1678 4702 4773 6804 1678 Size: 3024 71 2031 2531 Fragments arranged by size: 3024 2531 2031 71 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2629 2661 3155 3187 3311 Size: 32 135 32 1057 32 494 32 124 Cuts at: 3311 3343 3923 3955 4867 4899 6298 6330 6372 Size: 32 580 32 912 32 1399 32 42 Cuts at: 6372 6404 1373 Size: 32 2626 Fragments arranged by size: 2626 1399 1057 912 580 494 135 124 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 5001 5247 669 Size: 988 281 3063 246 3079 Fragments arranged by size: 3079 3063 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7657 HpaI GTT'AAC Cuts at: 5247 5247 Size: 7657 KasI G'GCGC_C Cuts at: 4698 4698 Size: 7657 KpnI G_GTAC'C Cuts at: 4982 4982 Size: 7657 MfeI C'AATT_G Cuts at: 2435 5256 2435 Size: 2821 4836 MluI A'CGCG_T Cuts at: 7533 7533 Size: 7657 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2037 2787 3059 6771 6955 1305 Size: 732 750 272 3712 184 2007 Fragments arranged by size: 3712 2007 750 732 272 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3219 10 Size: 54 1425 343 1387 4448 Fragments arranged by size: 4448 1425 1387 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2576 4257 5615 5974 6133 518 Size: 648 1410 1681 1358 359 159 2042 Fragments arranged by size: 2042 1681 1410 1358 648 359 159 MspA1I CmG'CkG Cuts at: 1606 5073 5750 6898 7143 1606 Size: 3467 677 1148 245 2120 Fragments arranged by size: 3467 2120 1148 677 245 NarI GG'CG_CC Cuts at: 4699 4699 Size: 7657 NcoI C'CATG_G Cuts at: 513 1065 2004 2521 513 Size: 552 939 517 5649 Fragments arranged by size: 5649 939 552 517 NdeI CA'TA_TG Cuts at: 387 387 Size: 7657 NheI G'CTAG_C Cuts at: 1052 3677 4709 1052 Size: 2625 1032 4000 Fragments arranged by size: 4000 2625 1032 NotI GC'GGCC_GC Cuts at: 5104 5104 Size: 7657 NspI r_CATG'y Cuts at: 1132 1229 3391 4182 4202 5015 6560 1132 Size: 97 2162 791 20 813 1545 2229 Fragments arranged by size: 2229 2162 1545 813 791 97 20 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7657 PciI A'CATG_T Cuts at: 6556 6556 Size: 7657 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7657 PfoI T'CCnGG_A Cuts at: 2544 2544 Size: 7657 PmeI GTTT'AAAC Cuts at: 4961 4961 Size: 7657 PmlI CAC'GTG Cuts at: 3290 3290 Size: 7657 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5726 5758 7239 7271 5726 Size: 32 1481 32 6112 Fragments arranged by size: 6112 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 4234 4234 Size: 7657 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7657 PsiI TTA'TAA Cuts at: 5227 5227 Size: 7657 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 4622 4654 4881 4913 4622 Size: 32 227 32 7366 Fragments arranged by size: 7366 227 32 32 PstI C_TGCA'G Cuts at: 830 2052 2307 5009 830 Size: 1222 255 2702 3478 Fragments arranged by size: 3478 2702 1222 255 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7657 SacI G_AGCT'C Cuts at: 3526 3526 Size: 7657 SalI G'TCGA_C Cuts at: 1936 4999 1936 Size: 3063 4594 SapI GCTCTTCn'nnn_ Cuts at: 3565 3565 Size: 7657 SbfI CC_TGCA'GG Cuts at: 5009 5009 Size: 7657 ScaI AGT'ACT Cuts at: 5905 7617 5905 Size: 1712 5945 SfcI C'TryA_G Cuts at: 826 1045 2048 2303 3582 3630 3687 4515 4647 Size: 219 1003 255 1279 48 57 828 132 Cuts at: 4647 5005 6140 6821 7012 826 Size: 358 1135 681 191 1471 Fragments arranged by size: 1471 1279 1135 1003 828 681 358 255 219 191 132 57 48 SfoI GGC'GCC Cuts at: 4700 4700 Size: 7657 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7657 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7657 SmaI CCC'GGG Cuts at: 1872 4984 1872 Size: 3112 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2775 3612 3713 3926 4089 4884 Size: 197 926 832 837 101 213 163 795 Cuts at: 4884 5761 6662 6924 820 Size: 877 901 262 1553 Fragments arranged by size: 1553 926 901 877 837 832 795 262 213 197 163 101 SnaBI TAC'GTA Cuts at: 493 5448 493 Size: 4955 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7657 SphI G_CATG'C Cuts at: 1132 5015 1132 Size: 3883 3774 SspI AAT'ATT Cuts at: 5 52 3538 3544 5581 5 Size: 47 3486 6 2037 2081 Fragments arranged by size: 3486 2081 2037 47 6 StuI AGG'CCT Cuts at: 1517 3900 1517 Size: 2383 5274 StyI C'CwwG_G Cuts at: 513 1065 2004 2360 2521 4531 4634 4702 4892 Size: 552 939 356 161 2010 103 68 190 Cuts at: 4892 5096 513 Size: 204 3074 Fragments arranged by size: 3074 2010 939 552 356 204 190 161 103 68 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4758 5684 5701 5854 6039 6484 325 Size: 1255 3178 926 17 153 185 445 1498 Fragments arranged by size: 3178 1498 1255 926 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2960 5903 7615 Size: 274 80 33 51 1443 983 2943 1712 Cuts at: 7615 96 Size: 138 Fragments arranged by size: 2943 1712 1443 983 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3073 3105 4068 4100 7617 7649 3073 Size: 32 963 32 3517 32 3081 Fragments arranged by size: 3517 3081 963 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7657 XbaI T'CTAG_A Cuts at: 4993 4993 Size: 7657 XhoI C'TCGA_G Cuts at: 1943 4089 4884 1943 Size: 2146 795 4716 Fragments arranged by size: 4716 2146 795 XmaI C'CCGG_G Cuts at: 1870 4982 1870 Size: 3112 4545 XmnI GAAnn'nnTTC Cuts at: 5786 5786 Size: 7657 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5465 276 Size: 53 83 186 936 3931 2468 Fragments arranged by size: 3931 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PsrI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BbvCI BfrBI BplI BsiWI BsmBI Bsu36I DraIII FseI FspAI NaeI NgoMIV NruI NsiI PacI PspOMI PspXI PvuII RsrII SacII SanDI SexAI SfiI SrfI SwaI XcmI