(Circular) (Six-base) MAPSORT of: pFN21AB6379.seq Check: 2938 from: 1 to: 5389 pFN21AB6379 5389 bp With 182 enzymes: SgfI * April 15, 2010 09:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2315 2315 Size: 5389 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2025 3199 278 Size: 53 83 186 936 489 1174 2468 Fragments arranged by size: 2468 1174 936 489 186 83 53 AccI GT'mk_AC Cuts at: 1937 2732 1937 Size: 795 4594 Acc65I G'GTAC_C Cuts at: 2710 2710 Size: 5389 AclI AA'CG_TT Cuts at: 1563 3516 3889 1563 Size: 1953 373 3063 Fragments arranged by size: 3063 1953 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2333 3452 4836 5104 1632 Size: 701 1119 1384 268 1917 Fragments arranged by size: 1917 1384 1119 701 268 AflII C'TTAA_G Cuts at: 820 1017 2113 2365 820 Size: 197 1096 252 3844 Fragments arranged by size: 3844 1096 252 197 AflIII A'CryG_T Cuts at: 1203 4288 5265 1203 Size: 3085 977 1327 Fragments arranged by size: 3085 1327 977 AgeI A'CCGG_T Cuts at: 4220 4220 Size: 5389 AhdI GACnn_n'nnGTC Cuts at: 4118 4118 Size: 5389 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5389 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5389 AlwNI CAG_nnn'CTG Cuts at: 2185 3132 4704 2185 Size: 947 1572 2870 Fragments arranged by size: 2870 1572 947 ApaLI G'TGCA_C Cuts at: 3446 4602 3446 Size: 1156 4233 ApoI r'AATT_y Cuts at: 1493 2057 2153 2531 2698 2924 3076 5185 1493 Size: 564 96 378 167 226 152 2109 1697 Fragments arranged by size: 2109 1697 564 378 226 167 152 96 AseI AT'TA_AT Cuts at: 160 1032 3943 160 Size: 872 2911 1606 Fragments arranged by size: 2911 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5389 AvaI C'yCGr_G Cuts at: 1870 1943 2714 1870 Size: 73 771 4545 Fragments arranged by size: 4545 771 73 BamHI G'GATC_C Cuts at: 2719 3086 2719 Size: 367 5022 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2549 2710 4165 618 Size: 325 217 627 762 161 1455 1842 Fragments arranged by size: 1842 1455 762 627 325 217 161 BanII G_rGCy'C Cuts at: 1391 2708 1391 Size: 1317 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4418 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3580 3614 1903 Size: 34 1643 34 3678 Fragments arranged by size: 3678 1643 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3281 4491 3281 Size: 1210 4179 BclI T'GATC_A Cuts at: 1548 1548 Size: 5389 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 4000 136 Size: 107 122 71 631 605 337 1991 1525 Fragments arranged by size: 1991 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 2359 5384 2359 Size: 3025 2364 BlpI GC'TnA_GC Cuts at: 2270 2806 2270 Size: 536 4853 Bme1580I G_kGCm'C Cuts at: 1790 2552 3450 4606 1790 Size: 762 898 1156 2573 Fragments arranged by size: 2573 1156 898 762 BmgBI CAC'GTC Cuts at: 1318 2029 1318 Size: 711 4678 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3120 4078 442 Size: 416 53 83 399 24 1703 958 1753 Fragments arranged by size: 1753 1703 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2633 1056 Size: 1577 3812 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2378 2378 Size: 5389 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2087 2207 2222 4049 1239 Size: 848 120 15 1827 2579 Fragments arranged by size: 2579 1827 848 120 15 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3514 4379 4677 3514 Size: 865 298 4226 Fragments arranged by size: 4226 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4052 882 Size: 617 2553 2219 Fragments arranged by size: 2553 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3180 493 Size: 711 1976 2702 Fragments arranged by size: 2702 1976 711 BsaBI GATnn'nnATC Cuts at: 5383 5383 Size: 5389 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2022 3196 3578 4213 Size: 53 83 186 936 489 1174 382 635 Cuts at: 4213 275 Size: 1451 Fragments arranged by size: 1451 1174 936 635 489 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2031 3822 4220 4494 4641 5052 2031 Size: 1791 398 274 147 411 2368 Fragments arranged by size: 2368 1791 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2220 2250 1178 Size: 30 165 30 817 30 4317 Fragments arranged by size: 4317 817 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2370 2489 1186 Size: 1184 119 4086 Fragments arranged by size: 4086 1184 119 BseYI C'CCAG_C Cuts at: 2454 2560 4592 5088 2454 Size: 106 2032 496 2755 Fragments arranged by size: 2755 2032 496 106 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4816 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2839 3600 4628 1540 Size: 275 186 838 761 1028 2301 Fragments arranged by size: 2301 1028 838 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2122 3450 3535 4606 2122 Size: 1328 85 1071 2905 Fragments arranged by size: 2905 1328 1071 85 BsmI GAATG_Cn' Cuts at: 2900 2993 2900 Size: 93 5296 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2122 2552 2708 3450 3535 4606 1391 Size: 399 332 430 156 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 430 399 332 156 85 BspEI T'CCGG_A Cuts at: 2031 5052 2031 Size: 3021 2368 BspHI T'CATG_A Cuts at: 3278 5008 3278 Size: 1730 3659 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2315 2449 2744 844 Size: 1471 134 295 3489 Fragments arranged by size: 3489 1471 295 134 BsrBI CCG'CTC Cuts at: 1394 1823 3276 1394 Size: 429 1453 3507 Fragments arranged by size: 3507 1453 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3884 4058 65 Size: 1183 2636 174 1396 Fragments arranged by size: 2636 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4033 4220 1700 Size: 110 2223 187 2869 Fragments arranged by size: 2869 2223 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5389 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5389 BssSI C'ACGA_G Cuts at: 3449 4461 3449 Size: 1012 4377 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5389 BstBI TT'CG_AA Cuts at: 4193 4193 Size: 5389 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5389 BstXI CCAn_nnnn'nTGG Cuts at: 2560 2560 Size: 5389 BstYI r'GATC_y Cuts at: 1972 2034 2359 2719 3086 3471 3488 4940 5026 Size: 62 325 360 367 385 17 1452 86 Cuts at: 5026 5038 5384 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 385 367 360 346 325 86 62 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3898 Fragments arranged by size: 3898 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4650 Fragments arranged by size: 4650 683 56 BtsI GCAGTG_nn' Cuts at: 2905 3699 3719 2905 Size: 794 20 4575 Fragments arranged by size: 4575 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5389 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5354 DraI TTT'AAA Cuts at: 2693 2693 Size: 5389 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2027 4396 809 Size: 507 711 2369 1802 Fragments arranged by size: 2369 1802 711 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2836 3725 5354 Size: 54 1425 50 275 18 1006 889 1629 Cuts at: 5354 8 Size: 43 Fragments arranged by size: 1629 1425 1006 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2836 1537 Size: 275 1024 4090 Fragments arranged by size: 4090 1024 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3319 1353 Size: 1966 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3976 4350 4496 387 Size: 1064 138 2387 374 146 1280 Fragments arranged by size: 2387 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2087 2207 2222 2333 3452 4049 4836 Size: 393 455 120 15 111 1119 597 787 Cuts at: 4836 5104 1239 Size: 268 1524 Fragments arranged by size: 1524 1119 787 597 455 393 268 120 111 15 EcoNI CCTnn'n_nnAGG Cuts at: 2287 2287 Size: 5389 EcoO109I rG'GnC_Cy Cuts at: 2108 2182 2108 Size: 74 5315 EcoRI G'AATT_C Cuts at: 2698 3076 2698 Size: 378 5011 EcoRV GAT'ATC Cuts at: 2092 2092 Size: 5389 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2360 2692 2360 Size: 332 5057 FspI TGC'GCA Cuts at: 3895 3895 Size: 5389 HaeII r_GCGC'y Cuts at: 1678 2081 4536 1678 Size: 403 2455 2531 Fragments arranged by size: 2531 2455 403 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2201 2233 4030 4062 4104 Size: 32 135 32 629 32 1797 32 42 Cuts at: 4104 4136 1373 Size: 32 2626 Fragments arranged by size: 2626 1797 629 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2733 2979 669 Size: 988 281 795 246 3079 Fragments arranged by size: 3079 988 795 281 246 HindIII A'AGCT_T Cuts at: 748 2570 2609 748 Size: 1822 39 3528 Fragments arranged by size: 3528 1822 39 HpaI GTT'AAC Cuts at: 2979 2979 Size: 5389 KpnI G_GTAC'C Cuts at: 2714 2714 Size: 5389 MfeI C'AATT_G Cuts at: 2988 2988 Size: 5389 MluI A'CGCG_T Cuts at: 5265 5265 Size: 5389 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4503 4687 1305 Size: 3198 184 2007 Fragments arranged by size: 3198 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3567 Fragments arranged by size: 3567 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3347 3706 3865 518 Size: 648 2181 359 159 2042 Fragments arranged by size: 2181 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2560 2805 3482 4630 4875 1606 Size: 954 245 677 1148 245 2120 Fragments arranged by size: 2120 1148 954 677 245 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3898 Fragments arranged by size: 3898 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5389 NheI G'CTAG_C Cuts at: 1052 2629 1052 Size: 1577 3812 NotI GC'GGCC_GC Cuts at: 2836 2836 Size: 5389 NspI r_CATG'y Cuts at: 1132 1229 2747 4292 1132 Size: 97 1518 1545 2229 Fragments arranged by size: 2229 1545 1518 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5389 PciI A'CATG_T Cuts at: 4288 4288 Size: 5389 PflMI CCAn_nnn'nTGG Cuts at: 1108 2245 1108 Size: 1137 4252 PfoI T'CCnGG_A Cuts at: 2238 2514 2238 Size: 276 5113 PmeI GTTT'AAAC Cuts at: 2693 2693 Size: 5389 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3458 3490 4971 5003 3458 Size: 32 1481 32 3844 Fragments arranged by size: 3844 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2108 2182 2108 Size: 74 5315 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5389 PsiI TTA'TAA Cuts at: 2959 2959 Size: 5389 PstI C_TGCA'G Cuts at: 830 2741 830 Size: 1911 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5389 PvuII CAG'CTG Cuts at: 2560 2560 Size: 5389 SalI G'TCGA_C Cuts at: 1936 2731 1936 Size: 795 4594 SbfI CC_TGCA'GG Cuts at: 2741 2741 Size: 5389 ScaI AGT'ACT Cuts at: 3637 5349 3637 Size: 1712 3677 SfcI C'TryA_G Cuts at: 826 1045 2737 3872 4553 4744 826 Size: 219 1692 1135 681 191 1471 Fragments arranged by size: 1692 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5389 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5389 SmaI CCC'GGG Cuts at: 1872 2716 1872 Size: 844 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2113 2365 3493 4394 4656 820 Size: 197 926 170 252 1128 901 262 1553 Fragments arranged by size: 1553 1128 926 901 262 252 197 170 SnaBI TAC'GTA Cuts at: 493 3180 493 Size: 2687 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5389 SphI G_CATG'C Cuts at: 1132 2747 1132 Size: 1615 3774 SspI AAT'ATT Cuts at: 5 52 3313 5 Size: 47 3261 2081 Fragments arranged by size: 3261 2081 47 StuI AGG'CCT Cuts at: 1517 2282 1517 Size: 765 4624 StyI C'CwwG_G Cuts at: 513 1065 2004 2828 513 Size: 552 939 824 3074 Fragments arranged by size: 3074 939 824 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3416 3433 3586 3771 4216 325 Size: 1255 1836 17 153 185 445 1498 Fragments arranged by size: 1836 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3635 5347 96 Size: 274 80 33 51 1443 1658 1712 138 Fragments arranged by size: 1712 1658 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2496 2528 5349 5381 2496 Size: 32 2821 32 2504 Fragments arranged by size: 2821 2504 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5389 XbaI T'CTAG_A Cuts at: 2329 2725 2329 Size: 396 4993 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2392 2392 Size: 5389 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5389 XmaI C'CCGG_G Cuts at: 1870 2714 1870 Size: 844 4545 XmnI GAAnn'nnTTC Cuts at: 2346 3518 2346 Size: 1172 4217 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2023 3197 276 Size: 53 83 186 936 489 1174 2468 Fragments arranged by size: 2468 1174 936 489 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI