Restriction map for
pFN21AB8528
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# NO CUT ENZYMES ( 88 enzymes) #
##################################
AccI AclI AflII AgeI Alw44I
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I AviII AvrII BamHI
BbrPI BcgI BciVI BclI BfrI
BglI BglII BlnI BsaAI BsaI
BsiEI BsiWI BsmI BspDI BspHI
BssHII Bst1107I BstEII BstPI BstXI
BstZ17I ClaI DraI DraIII EagI
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoRI EcoRV FbaI FseI FspI
HindIII HpaI KspI MfeI MluI
MslI MunI NdeI NheI NotI
NruI PacI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI SacI SacII SalI SbfI
ScaI SexAI SfiI SgrAI SnaBI
SphI Sse8387I SspI StuI SwaI
XbaI XcmI XmnI
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# ONE CUT ENZYMES ( 61 enzymes) #
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AatII 694 GACGTC GACGT^C
Acc65I 1942 GGTACC G^GTACC
AccII 1372 CGCG CG^CG
AccIII 1848 TCCGGA T^CCGGA
AhdI 2303 GACAGCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 1531 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp718I 1942 GGTACC G^GTACC
AspEI 2303 GACAGCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2535 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 2644 TGGCCA TGG^CCA
BanII 22 GAGCCC G[GA]GC[CT]^C
BbeI 3 GGCGCC GGCGC^C
BbvCI 547 CCTCAGC CCTCAGC(-5/-2)
BsaWI 1848 TCCGGA [AT]^CCGG[AT]
BseAI 1848 TCCGGA T^CCGGA
BseRI 1291 GAGGAG GAGGAG(10/8)
BsmBI 2112 CGTCTC CGTCTC(1/5)
Bsp1407I 1046 TGTACA T^GTACA
BspEI 1848 TCCGGA T^CCGGA
BsrBI 1776 GAGCGG CCGCTC(-3/-3)
BsrDI 2556 CATTGC GCAATG(2/0)
BsrFI 430 GCCGGC [GA]^CCGG[CT]
BsrGI 1046 TGTACA T^GTACA
BssSI 889 CTCGTG CACGAG(-5/-1)
BstBI 1034 TTCGAA TT^CGAA
BstUI 1372 CGCG CG^CG
BstYI 1879 GGATCT [GA]^GATC[CT]
Cfr10I 430 GCCGGC [GA]^CCGG[CT]
CpoI 1779 CGGACCG CG^G[AT]CCG
DrdI 688 GACTTGGACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 2644 TGGCCA [CT]^GGCC[GA]
Eam1105I 2303 GACAGCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT22I 2553 ATGCAT ATGCA^T
FauI 660 GCGGG CCCGC(4/6)
HaeII 3 GGCGCC [GA]GCGC^[CT]
HgaI 91 GACGC GACGC(5/10)
HphI 137 TCACC GGTGA(8/7)
KasI 3 GGCGCC G^GCGCC
KpnI 1942 GGTACC GGTAC^C
MflI 1879 GGATCT [GA]^GATC[CT]
MluNI 2644 TGGCCA TGG^CCA
MroI 1848 TCCGGA T^CCGGA
MscI 2644 TGGCCA TGG^CCA
MvnI 1372 CGCG CG^CG
NaeI 430 GCCGGC GCC^GGC
NarI 3 GGCGCC GG^CGCC
NcoI 270 CCATGG C^CATGG
NgoMIV 430 GCCGGC G^CCGGC
NsiI 2553 ATGCAT ATGCA^T
NspV 1034 TTCGAA TT^CGAA
PaeR7I 1027 CTCGAG C^TCGAG
PflFI 2535 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
RsrII 1779 CGGACCG CG^G[AT]CCG
SapI 1773 GAAGAGC GCTCTTC(1/4)
SfoI 3 GGCGCC GGC^GCC
SfuI 1034 TTCGAA TT^CGAA
SpeI 336 ACTAGT A^CTAGT
SspBI 1046 TGTACA T^GTACA
Tth111I 2535 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 1027 CTCGAG C^TCGAG
XhoII 1879 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AspHI 943 GAGCAC G[AT]GC[AT]^C
2105 GTGCTC
BanI 3 GGCGCC G^G[CT][GA]CC
1942 GGTACC
BlpI 1299 GCTCAGC GC^T[ACGT]AGC
2230 GCTGAGC
Bpu1102I 1299 GCTCAGC GC^T[ACGT]AGC
2230 GCTGAGC
BsaBI 1162 GATACTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1852 GATGGCTATC
BsgI 704 CTGCAC GTGCAG(16/14)
1390 GTGCAG
BsiHKAI 943 GAGCAC G[AT]GC[AT]^C
2105 GTGCTC
CelII 1299 GCTCAGC GC^T[ACGT]AGC
2230 GCTGAGC
DsaI 125 CCGTGG C^C[GA][CT]GG
270 CCATGG
HincII 934 GTTGAC GT[CT]^[GA]AC
1518 GTTGAC
HindII 934 GTTGAC GT[CT]^[GA]AC
1518 GTTGAC
MamI 1162 GATACTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1852 GATGGCTATC
PflMI 160 CCACAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1870 CCAACCAGTGG
PstI 1396 CTGCAG CTGCA^G
2276 CTGCAG
TfiI 408 GAATC G^A[AT]TC
1105 GATTC
Van91I 160 CCACAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1870 CCAACCAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 93 enzymes) #
#########################################
AciI AcsI AcyI AfaI AflIII
AluI AlwI AocI ApoI AvaI
AvaII BbsI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaHI BsaJI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspLU11I BspMI BsrI BssKI
BstAPI BstF5I BstNI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraII
EarI Eco81I EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HhaI
Hin1I HinP1I HinfI HpaII ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MnlI MseI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PpuMI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI StyI TaqI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XmaCI XmaI