Restriction map for
pFN21AB8528
(Circular) (Six-base) MAPSORT of: pFN21AB8528.seq Check: 5443 from: 1 to: 7360
pFN21AB8528 7360 bp
With 182 enzymes: SgfI *
April 15, 2010 09:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3394 3394
Size: 7360
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2703 5170 278
Size: 53 83 186 936 1167 2467 2468
Fragments arranged by size:
2468 2467 1167 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4703 1937
Size: 2766 4594
Acc65I G'GTAC_C
Cuts at: 3947 4681 3947
Size: 734 6626
AclI AA'CG_TT
Cuts at: 1563 5487 5860 1563
Size: 3924 373 3063
Fragments arranged by size:
3924 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2231 3648 3777 4023 4621 5423 6807 7075
Size: 599 1417 129 246 598 802 1384 268
Cuts at: 7075 1632
Size: 1917
Fragments arranged by size:
1917 1417 1384 802 599 598 268 246
129
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7163
AflIII A'CryG_T
Cuts at: 1203 2760 3048 3159 6259 7236 1203
Size: 1557 288 111 3100 977 1327
Fragments arranged by size:
3100 1557 1327 977 288 111
AgeI A'CCGG_T
Cuts at: 6191 6191
Size: 7360
AhdI GACnn_n'nnGTC
Cuts at: 4313 6089 4313
Size: 1776 5584
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7360
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2282 4263 1184
Size: 1098 1981 4281
Fragments arranged by size:
4281 1981 1098
AlwNI CAG_nnn'CTG
Cuts at: 3541 5103 6675 3541
Size: 1562 1572 4226
Fragments arranged by size:
4226 1572 1562
ApaLI G'TGCA_C
Cuts at: 5417 6573 5417
Size: 1156 6204
ApoI r'AATT_y
Cuts at: 1493 2930 2994 3443 4404 4669 4895 5047 7156
Size: 1437 64 449 961 265 226 152 2109
Cuts at: 7156 1493
Size: 1697
Fragments arranged by size:
2109 1697 1437 961 449 265 226 152
64
AseI AT'TA_AT
Cuts at: 160 1032 5914 160
Size: 872 4882 1606
Fragments arranged by size:
4882 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7360
AvaI C'yCGr_G
Cuts at: 1870 1943 2022 3032 3293 3315 3732 4251 4685
Size: 73 79 1010 261 22 417 519 434
Cuts at: 4685 1870
Size: 4545
Fragments arranged by size:
4545 1010 519 434 417 261 79 73
22
BamHI G'GATC_C
Cuts at: 4690 5057 4690
Size: 367 6993
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2008 3947 4681 6136 618
Size: 325 217 627 221 1939 734 1455 1842
Fragments arranged by size:
1939 1842 1455 734 627 325 221 217
BanII G_rGCy'C
Cuts at: 1391 2031 4679 1391
Size: 640 2648 4072
Fragments arranged by size:
4072 2648 640
BbeI G_GCGC'C
Cuts at: 2012 2012
Size: 7360
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2331 3018 4011 928
Size: 971 432 687 993 4277
Fragments arranged by size:
4277 993 971 687 432
BbvCI CC'TCA_GC
Cuts at: 2553 2553
Size: 7360
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5551 5585 1903
Size: 34 3614 34 3678
Fragments arranged by size:
3678 3614 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5252 6462 5252
Size: 1210 6150
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7360
BfrBI ATG'CAT
Cuts at: 4560 4560
Size: 7360
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5971 136
Size: 107 122 71 631 605 4299 1525
Fragments arranged by size:
4299 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7355 7355
Size: 7360
BlpI GC'TnA_GC
Cuts at: 3305 4236 4777 3305
Size: 931 541 5888
Fragments arranged by size:
5888 931 541
Bme1580I G_kGCm'C
Cuts at: 1790 5421 6577 1790
Size: 3631 1156 2573
Fragments arranged by size:
3631 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7360
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2010 2830 3668
Size: 416 53 83 399 24 593 820 838
Cuts at: 3668 3789 4109 5091 6049 442
Size: 121 320 982 958 1753
Fragments arranged by size:
1753 982 958 838 820 593 416 399
320 121 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7360
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2971 2971
Size: 7360
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2387 3383 3571 6020 1239
Size: 1148 996 188 2449 2579
Fragments arranged by size:
2579 2449 1148 996 188
Bpu10I CC'TnA_GC
Cuts at: 2553 2553
Size: 7360
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2543 2628 3422 3734 5485 6350 6648 2543
Size: 85 794 312 1751 865 298 3255
Fragments arranged by size:
3255 1751 865 794 312 298 85
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6023 882
Size: 617 4524 2219
Fragments arranged by size:
4524 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5151 493
Size: 711 3947 2702
Fragments arranged by size:
3947 2702 711
BsaBI GATnn'nnATC
Cuts at: 3171 3861 7354 3171
Size: 690 3493 3177
Fragments arranged by size:
3493 3177 690
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2009 2097 2700 5167
Size: 53 83 186 936 476 88 603 2467
Cuts at: 5167 5549 6184 275
Size: 382 635 1451
Fragments arranged by size:
2467 1451 936 635 603 476 382 186
88 83 53
BsaWI w'CCGG_w
Cuts at: 3853 5793 6191 6465 6612 7023 3853
Size: 1940 398 274 147 411 4190
Fragments arranged by size:
4190 1940 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2061 2091 3824 3854 1178
Size: 30 165 30 658 30 1733 30 4684
Fragments arranged by size:
4684 1733 658 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3311 1186
Size: 2125 5235
BseYI C'CCAG_C
Cuts at: 2742 2803 3079 3943 6563 7059 2742
Size: 61 276 864 2620 496 3043
Fragments arranged by size:
3043 2620 864 496 276 61
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2694 3416 1163
Size: 573 958 722 5107
Fragments arranged by size:
5107 958 722 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4810 5571 6599 1540
Size: 275 186 2809 761 1028 2301
Fragments arranged by size:
2809 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2952 4114 5421 5506 6577 2952
Size: 1162 1307 85 1071 3735
Fragments arranged by size:
3735 1307 1162 1071 85
BsmI GAATG_Cn'
Cuts at: 4871 4964 4871
Size: 93 7267
BsmBI CGTCTCn'nnnn_
Cuts at: 4123 4123
Size: 7360
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2031 2952 4114 4679 5421 5506 6577
Size: 399 241 921 1162 565 742 85 1071
Cuts at: 6577 1391
Size: 2174
Fragments arranged by size:
2174 1162 1071 921 742 565 399 241
85
BspEI T'CCGG_A
Cuts at: 3853 7023 3853
Size: 3170 4190
BspHI T'CATG_A
Cuts at: 5249 6979 5249
Size: 1730 5630
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2434 2737 2990 3342 3394 4303 4715 844
Size: 1590 303 253 352 52 909 412 3489
Fragments arranged by size:
3489 1590 909 412 352 303 253 52
BsrBI CCG'CTC
Cuts at: 1394 1823 3783 5247 1394
Size: 429 1960 1464 3507
Fragments arranged by size:
3507 1960 1464 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4560 5855 6029 65
Size: 1183 3312 1295 174 1396
Fragments arranged by size:
3312 1396 1295 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2435 6004 6191 1700
Size: 110 625 3569 187 2869
Fragments arranged by size:
3569 2869 625 187 110
BsrGI T'GTAC_A
Cuts at: 96 3051 96
Size: 2955 4405
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7360
BssSI C'ACGA_G
Cuts at: 2894 5420 6432 2894
Size: 2526 1012 3822
Fragments arranged by size:
3822 2526 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3394 4318 4557 794
Size: 2600 924 239 3597
Fragments arranged by size:
3597 2600 924 239
BstBI TT'CG_AA
Cuts at: 3040 6164 3040
Size: 3124 4236
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7360
BstYI r'GATC_y
Cuts at: 1972 3884 4690 5057 5442 5459 6911 6997 7009
Size: 1912 806 367 385 17 1452 86 12
Cuts at: 7009 7355 1972
Size: 346 1977
Fragments arranged by size:
1977 1912 1452 806 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2398 2688 3639 3648 2398
Size: 290 951 9 6110
Fragments arranged by size:
6110 951 290 9
BtgI C'CryG_G
Cuts at: 513 1065 2004 2130 2275 513
Size: 552 939 126 145 5598
Fragments arranged by size:
5598 939 552 145 126
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2190 487
Size: 683 56 964 5657
Fragments arranged by size:
5657 964 683 56
BtsI GCAGTG_nn'
Cuts at: 2820 4876 5670 5690 2820
Size: 2056 794 20 4490
Fragments arranged by size:
4490 2056 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7360
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7325
DraI TTT'AAA
Cuts at: 4664 4664
Size: 7360
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2699 6367 809
Size: 507 1383 3668 1802
Fragments arranged by size:
3668 1802 1383 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4649 4807 5696
Size: 54 1425 50 275 18 2819 158 889
Cuts at: 5696 7325 8
Size: 1629 43
Fragments arranged by size:
2819 1629 1425 889 275 158 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4807 1537
Size: 275 2995 4090
Fragments arranged by size:
4090 2995 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2850 3773 3920 4334 5290 1353
Size: 1497 923 147 414 956 3423
Fragments arranged by size:
3423 1497 956 923 414 147
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5947 6321 6467 387
Size: 1064 138 4358 374 146 1280
Fragments arranged by size:
4358 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2231 2387 3383 3571 3648 3777 4023
Size: 393 599 156 996 188 77 129 246
Cuts at: 4023 4621 5423 6020 6807 7075 1239
Size: 598 802 597 787 268 1524
Fragments arranged by size:
1524 996 802 787 599 598 597 393
268 246 188 156 129 77
EcoO109I rG'GnC_Cy
Cuts at: 2035 2158 2446 4423 2035
Size: 123 288 1977 4972
Fragments arranged by size:
4972 1977 288 123
EcoRI G'AATT_C
Cuts at: 4669 5047 4669
Size: 378 6982
FspI TGC'GCA
Cuts at: 5866 5866
Size: 7360
HaeII r_GCGC'y
Cuts at: 1678 2012 6507 1678
Size: 334 4495 2531
Fragments arranged by size:
4495 2531 334
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2381 2413 2541 2573 4299
Size: 32 135 32 809 32 128 32 1726
Cuts at: 4299 4331 6001 6033 6075 6107 1373
Size: 32 1670 32 42 32 2626
Fragments arranged by size:
2626 1726 1670 809 135 128 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2941 3525 4704 4950 669
Size: 988 281 1003 584 1179 246 3079
Fragments arranged by size:
3079 1179 1003 988 584 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7360
HpaI GTT'AAC
Cuts at: 4950 4950
Size: 7360
KasI G'GCGC_C
Cuts at: 2008 2008
Size: 7360
KpnI G_GTAC'C
Cuts at: 3951 4685 3951
Size: 734 6626
MfeI C'AATT_G
Cuts at: 4959 4959
Size: 7360
MluI A'CGCG_T
Cuts at: 7236 7236
Size: 7360
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2088 2572 2610 4154 4330 4637 6474 6658
Size: 783 484 38 1544 176 307 1837 184
Cuts at: 6658 1305
Size: 2007
Fragments arranged by size:
2007 1837 1544 783 484 307 184 176
38
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4651 10
Size: 54 1425 343 2819 2719
Fragments arranged by size:
2819 2719 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 5318 5677 5836 518
Size: 648 4152 359 159 2042
Fragments arranged by size:
4152 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2469 3265 3400 3538 3790 3971 4235 4247
Size: 863 796 135 138 252 181 264 12
Cuts at: 4247 4776 5453 6601 6846 1606
Size: 529 677 1148 245 2120
Fragments arranged by size:
2120 1148 863 796 677 529 264 252
245 181 138 135 12
NaeI GCC'GGC
Cuts at: 2437 2437
Size: 7360
NarI GG'CG_CC
Cuts at: 2009 2009
Size: 7360
NcoI C'CATG_G
Cuts at: 513 1065 2004 2275 513
Size: 552 939 271 5598
Fragments arranged by size:
5598 939 552 271
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7360
NgoMIV G'CCGG_C
Cuts at: 2435 2435
Size: 7360
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7360
NotI GC'GGCC_GC
Cuts at: 4807 4807
Size: 7360
NsiI A_TGCA'T
Cuts at: 4562 4562
Size: 7360
NspI r_CATG'y
Cuts at: 1132 1229 2764 3052 3163 3957 4606 4718 6263
Size: 97 1535 288 111 794 649 112 1545
Cuts at: 6263 1132
Size: 2229
Fragments arranged by size:
2229 1545 1535 794 649 288 112 111
97
PasI CC'CwG_GG
Cuts at: 1348 2031 1348
Size: 683 6677
PciI A'CATG_T
Cuts at: 2760 3048 3159 6259 2760
Size: 288 111 3100 3861
Fragments arranged by size:
3861 3100 288 111
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2171 3881 1108
Size: 1063 1710 4587
Fragments arranged by size:
4587 1710 1063
PfoI T'CCnGG_A
Cuts at: 2091 2091
Size: 7360
PmeI GTTT'AAAC
Cuts at: 4664 4664
Size: 7360
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5429 5461 6942 6974 5429
Size: 32 1481 32 5815
Fragments arranged by size:
5815 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2035 2446 4423 2035
Size: 411 1977 4972
Fragments arranged by size:
4972 1977 411
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7360
PsiI TTA'TAA
Cuts at: 4930 4930
Size: 7360
PspXI vC'TCGA_Gb
Cuts at: 3032 3032
Size: 7360
PstI C_TGCA'G
Cuts at: 830 3405 4285 4712 830
Size: 2575 880 427 3478
Fragments arranged by size:
3478 2575 880 427
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7360
PvuII CAG'CTG
Cuts at: 2469 3265 3400 3538 3971 4235 4247 2469
Size: 796 135 138 433 264 12 5582
Fragments arranged by size:
5582 796 433 264 138 135 12
RsrII CG'GwC_CG
Cuts at: 3785 3785
Size: 7360
SalI G'TCGA_C
Cuts at: 1936 4702 1936
Size: 2766 4594
SapI GCTCTTCn'nnn_
Cuts at: 3773 3773
Size: 7360
SbfI CC_TGCA'GG
Cuts at: 4712 4712
Size: 7360
ScaI AGT'ACT
Cuts at: 5608 7320 5608
Size: 1712 5648
SfcI C'TryA_G
Cuts at: 826 1045 2850 3128 3401 3487 4281 4462 4708
Size: 219 1805 278 273 86 794 181 246
Cuts at: 4708 5843 6524 6715 826
Size: 1135 681 191 1471
Fragments arranged by size:
1805 1471 1135 794 681 278 273 246
219 191 181 86
SfoI GGC'GCC
Cuts at: 2010 2010
Size: 7360
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7360
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7360
SmaI CCC'GGG
Cuts at: 1872 2024 3317 4253 4687 1872
Size: 152 1293 936 434 4545
Fragments arranged by size:
4545 1293 936 434 152
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2558 2643 3032 3437 3749 5464
Size: 197 926 615 85 389 405 312 1715
Cuts at: 5464 6365 6627 820
Size: 901 262 1553
Fragments arranged by size:
1715 1553 926 901 615 405 389 312
262 197 85
SnaBI TAC'GTA
Cuts at: 493 5151 493
Size: 4658 2702
SpeI A'CTAG_T
Cuts at: 152 2341 152
Size: 2189 5171
SphI G_CATG'C
Cuts at: 1132 4718 1132
Size: 3586 3774
SspI AAT'ATT
Cuts at: 5 52 5284 5
Size: 47 5232 2081
Fragments arranged by size:
5232 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7360
StyI C'CwwG_G
Cuts at: 513 1065 2004 2145 2275 4364 4799 513
Size: 552 939 141 130 2089 435 3074
Fragments arranged by size:
3074 2089 939 552 435 141 130
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2029 2268 3341 5387 5404 5557 5742
Size: 1255 449 239 1073 2046 17 153 185
Cuts at: 5742 6187 325
Size: 445 1498
Fragments arranged by size:
2046 1498 1255 1073 449 445 239 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3051 3595 4195
Size: 274 80 33 51 1443 1074 544 600
Cuts at: 4195 5606 7318 96
Size: 1411 1712 138
Fragments arranged by size:
1712 1443 1411 1074 600 544 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2061 2093 3824 3856 7320 7352 2061
Size: 32 1731 32 3464 32 2069
Fragments arranged by size:
3464 2069 1731 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4543 1316
Size: 3227 4133
XbaI T'CTAG_A
Cuts at: 4696 4696
Size: 7360
XhoI C'TCGA_G
Cuts at: 1943 3032 1943
Size: 1089 6271
XmaI C'CCGG_G
Cuts at: 1870 2022 3315 4251 4685 1870
Size: 152 1293 936 434 4545
Fragments arranged by size:
4545 1293 936 434 152
XmnI GAAnn'nnTTC
Cuts at: 5489 5489
Size: 7360
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2701 5168 276
Size: 53 83 186 936 1167 2467 2468
Fragments arranged by size:
2468 2467 1167 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspXI PstI PvuI PvuII RsrII SalI SapI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BsiWI BstXI
BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI
NruI PacI PmlI PspOMI PsrI SacI SacII SanDI
SexAI SfiI SrfI SwaI XcmI