Restriction map for
pFN21AB8528
(Linear) (Six-base) MAPSORT of: KIBB8528.seq Check: 3815 from: 1 to: 2655
KIBB8528.orf
With 182 enzymes: SgfI *
October 7, 2009 14:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1389 2655
Size: 1389 1266
AatII G_ACGT'C
Cuts at: 0 698 2655
Size: 698 1957
Acc65I G'GTAC_C
Cuts at: 0 1942 2655
Size: 1942 713
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 226 1643 1772 2018 2616 2655
Size: 226 1417 129 246 598 39
Fragments arranged by size:
1417 598 246 226 129 39
AflIII A'CryG_T
Cuts at: 0 755 1043 1154 2655
Size: 755 288 111 1501
Fragments arranged by size:
1501 755 288 111
AhdI GACnn_n'nnGTC
Cuts at: 0 2308 2655
Size: 2308 347
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 277 2258 2655
Size: 277 1981 397
Fragments arranged by size:
1981 397 277
AlwNI CAG_nnn'CTG
Cuts at: 0 1536 2655
Size: 1536 1119
ApoI r'AATT_y
Cuts at: 0 925 989 1438 2399 2655
Size: 925 64 449 961 256
Fragments arranged by size:
961 925 449 256 64
AvaI C'yCGr_G
Cuts at: 0 17 1027 1288 1310 1727 2246 2655
Size: 17 1010 261 22 417 519 409
Fragments arranged by size:
1010 519 417 409 261 22 17
BanI G'GyrC_C
Cuts at: 0 3 1942 2655
Size: 3 1939 713
Fragments arranged by size:
1939 713 3
BanII G_rGCy'C
Cuts at: 0 26 2655
Size: 26 2629
BbeI G_GCGC'C
Cuts at: 0 7 2655
Size: 7 2648
BbsI GAAGACnn'nnnn_
Cuts at: 0 326 1013 2006 2655
Size: 326 687 993 649
Fragments arranged by size:
993 687 649 326
BbvCI CC'TCA_GC
Cuts at: 0 548 2655
Size: 548 2107
BfrBI ATG'CAT
Cuts at: 0 2555 2655
Size: 2555 100
BlpI GC'TnA_GC
Cuts at: 0 1300 2231 2655
Size: 1300 931 424
Fragments arranged by size:
1300 931 424
BmrI ACTGGGnnnn_n'
Cuts at: 0 5 825 1663 1784 2104 2655
Size: 5 820 838 121 320 551
Fragments arranged by size:
838 820 551 320 121 5
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 966 2655
Size: 966 1689
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 382 1378 1566 2655
Size: 382 996 188 1089
Fragments arranged by size:
1089 996 382 188
Bpu10I CC'TnA_GC
Cuts at: 0 548 2655
Size: 548 2107
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 538 623 1417 1729 2655
Size: 538 85 794 312 926
Fragments arranged by size:
926 794 538 312 85
BsaBI GATnn'nnATC
Cuts at: 0 1166 1856 2655
Size: 1166 690 799
Fragments arranged by size:
1166 799 690
BsaHI Gr'CG_yC
Cuts at: 0 4 92 695 2655
Size: 4 88 603 1960
Fragments arranged by size:
1960 603 88 4
BsaWI w'CCGG_w
Cuts at: 0 1848 2655
Size: 1848 807
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 56 86 1819 1849 2655
Size: 56 30 1733 30 806
Fragments arranged by size:
1733 806 56 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1306 2655
Size: 1306 1349
BseYI C'CCAG_C
Cuts at: 0 737 798 1074 1938 2655
Size: 737 61 276 864 717
Fragments arranged by size:
864 737 717 276 61
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 689 1411 2655
Size: 689 722 1244
Fragments arranged by size:
1244 722 689
BsiHKAI G_wGCw'C
Cuts at: 0 947 2109 2655
Size: 947 1162 546
Fragments arranged by size:
1162 947 546
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2118 2655
Size: 2118 537
Bsp1286I G_dGCh'C
Cuts at: 0 26 947 2109 2655
Size: 26 921 1162 546
Fragments arranged by size:
1162 921 546 26
BspEI T'CCGG_A
Cuts at: 0 1848 2655
Size: 1848 807
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 429 732 985 1337 1389 2298 2655
Size: 429 303 253 352 52 909 357
Fragments arranged by size:
909 429 357 352 303 253 52
BsrBI CCG'CTC
Cuts at: 0 1778 2655
Size: 1778 877
BsrDI GCAATG_nn'
Cuts at: 0 2555 2655
Size: 2555 100
BsrFI r'CCGG_y
Cuts at: 0 430 2655
Size: 430 2225
BsrGI T'GTAC_A
Cuts at: 0 1046 2655
Size: 1046 1609
BssSI C'ACGA_G
Cuts at: 0 889 2655
Size: 889 1766
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1389 2313 2552 2655
Size: 1389 924 239 103
Fragments arranged by size:
1389 924 239 103
BstBI TT'CG_AA
Cuts at: 0 1035 2655
Size: 1035 1620
BstYI r'GATC_y
Cuts at: 0 1879 2655
Size: 1879 776
Bsu36I CC'TnA_GG
Cuts at: 0 393 683 1634 1643 2655
Size: 393 290 951 9 1012
Fragments arranged by size:
1012 951 393 290 9
BtgI C'CryG_G
Cuts at: 0 125 270 2655
Size: 125 145 2385
Fragments arranged by size:
2385 145 125
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 185 2655
Size: 185 2470
BtsI GCAGTG_nn'
Cuts at: 0 815 2655
Size: 815 1840
DrdI GACnn_nn'nnGTC
Cuts at: 0 694 2655
Size: 694 1961
EaeI y'GGCC_r
Cuts at: 0 2644 2655
Size: 2644 11
EarI CTCTTCn'nnn_
Cuts at: 0 845 1768 1915 2329 2655
Size: 845 923 147 414 326
Fragments arranged by size:
923 845 414 326 147
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 226 382 1378 1566 1643 1772 2018 2616
Size: 226 156 996 188 77 129 246 598
Cuts at: 2616 2655
Size: 39
Fragments arranged by size:
996 598 246 226 188 156 129 77
39
EcoO109I rG'GnC_Cy
Cuts at: 0 30 153 441 2418 2655
Size: 30 123 288 1977 237
Fragments arranged by size:
1977 288 237 123 30
HaeII r_GCGC'y
Cuts at: 0 7 2655
Size: 7 2648
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 376 408 536 568 2294 2326 2655
Size: 376 32 128 32 1726 32 329
Fragments arranged by size:
1726 376 329 128 32 32 32
HincII GTy'rAC
Cuts at: 0 936 1520 2655
Size: 936 584 1135
Fragments arranged by size:
1135 936 584
KasI G'GCGC_C
Cuts at: 0 3 2655
Size: 3 2652
KpnI G_GTAC'C
Cuts at: 0 1946 2655
Size: 1946 709
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 83 567 605 2149 2325 2632 2655
Size: 83 484 38 1544 176 307 23
Fragments arranged by size:
1544 484 307 176 83 38 23
MscI TGG'CCA
Cuts at: 0 2646 2655
Size: 2646 9
MspA1I CmG'CkG
Cuts at: 0 464 1260 1395 1533 1785 1966 2230 2242
Size: 464 796 135 138 252 181 264 12
Cuts at: 2242 2655
Size: 413
Fragments arranged by size:
796 464 413 264 252 181 138 135
12
NaeI GCC'GGC
Cuts at: 0 432 2655
Size: 432 2223
NarI GG'CG_CC
Cuts at: 0 4 2655
Size: 4 2651
NcoI C'CATG_G
Cuts at: 0 270 2655
Size: 270 2385
NgoMIV G'CCGG_C
Cuts at: 0 430 2655
Size: 430 2225
NsiI A_TGCA'T
Cuts at: 0 2557 2655
Size: 2557 98
NspI r_CATG'y
Cuts at: 0 759 1047 1158 1952 2601 2655
Size: 759 288 111 794 649 54
Fragments arranged by size:
794 759 649 288 111 54
PasI CC'CwG_GG
Cuts at: 0 26 2655
Size: 26 2629
PciI A'CATG_T
Cuts at: 0 755 1043 1154 2655
Size: 755 288 111 1501
Fragments arranged by size:
1501 755 288 111
PflMI CCAn_nnn'nTGG
Cuts at: 0 166 1876 2655
Size: 166 1710 779
Fragments arranged by size:
1710 779 166
PfoI T'CCnGG_A
Cuts at: 0 86 2655
Size: 86 2569
PpuMI rG'GwC_Cy
Cuts at: 0 30 441 2418 2655
Size: 30 411 1977 237
Fragments arranged by size:
1977 411 237 30
PspXI vC'TCGA_Gb
Cuts at: 0 1027 2655
Size: 1027 1628
PstI C_TGCA'G
Cuts at: 0 1400 2280 2655
Size: 1400 880 375
Fragments arranged by size:
1400 880 375
PvuII CAG'CTG
Cuts at: 0 464 1260 1395 1533 1966 2230 2242 2655
Size: 464 796 135 138 433 264 12 413
Fragments arranged by size:
796 464 433 413 264 138 135 12
RsrII CG'GwC_CG
Cuts at: 0 1780 2655
Size: 1780 875
SapI GCTCTTCn'nnn_
Cuts at: 0 1768 2655
Size: 1768 887
SfcI C'TryA_G
Cuts at: 0 845 1123 1396 1482 2276 2457 2655
Size: 845 278 273 86 794 181 198
Fragments arranged by size:
845 794 278 273 198 181 86
SfoI GGC'GCC
Cuts at: 0 5 2655
Size: 5 2650
SmaI CCC'GGG
Cuts at: 0 19 1312 2248 2655
Size: 19 1293 936 407
Fragments arranged by size:
1293 936 407 19
SmlI C'TyrA_G
Cuts at: 0 553 638 1027 1432 1744 2655
Size: 553 85 389 405 312 911
Fragments arranged by size:
911 553 405 389 312 85
SpeI A'CTAG_T
Cuts at: 0 336 2655
Size: 336 2319
StyI C'CwwG_G
Cuts at: 0 140 270 2359 2655
Size: 140 130 2089 296
Fragments arranged by size:
2089 296 140 130
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 24 263 1336 2655
Size: 24 239 1073 1319
Fragments arranged by size:
1319 1073 239 24
TatI w'GTAC_w
Cuts at: 0 1046 1590 2190 2655
Size: 1046 544 600 465
Fragments arranged by size:
1046 600 544 465
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 56 88 1819 1851 2655
Size: 56 32 1731 32 804
Fragments arranged by size:
1731 804 56 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 2538 2655
Size: 2538 117
XhoI C'TCGA_G
Cuts at: 0 1027 2655
Size: 1027 1628
XmaI C'CCGG_G
Cuts at: 0 17 1310 2246 2655
Size: 17 1293 936 409
Fragments arranged by size:
1293 936 409 17
ZraI GAC'GTC
Cuts at: 0 696 2655
Size: 696 1959
Enzymes that do cut:
AarI AatII Acc65I AcuI AflIII AhdI AlfI AlwNI
ApoI AvaI BanI BanII BbeI BbsI BbvCI BfrBI
BlpI BmrI BplI BpmI Bpu10I BpuEI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I
BspEI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstYI Bsu36I BtgI BtgZI BtsI DrdI EaeI
EarI Eco57MI EcoO109I HaeII Hin4I HincII KasI KpnI
MmeI MscI MspA1I NaeI NarI NcoI NgoMIV NsiI
NspI PasI PciI PflMI PfoI PpuMI PspXI PstI
PvuII RsrII SapI SfcI SfoI SmaI SmlI SpeI
StyI TaqII TatI TstI Tth111I XhoI XmaI ZraI
Enzymes that do not cut:
AccI AclI AfeI AflII AgeI AleI AloI ApaI
ApaLI AscI AseI AsiSI AvrII BaeI BamHI BcgI
BciVI BclI BglI BglII Bme1580I BmgBI BmtI BsaI
BsaAI BsiEI BsiWI BsmI BspHI BssHII BstEII BstXI
BstZ17I ClaI CspCI DraI DraIII EagI EciI EcoICRI
EcoNI EcoRI EcoRV FalI FseI FspI FspAI HindIII
HpaI MfeI MluI MslI NdeI NheI NotI NruI
PacI PmeI PmlI PpiI PshAI PsiI PspOMI PsrI
PvuI SacI SacII SalI SanDI SbfI ScaI SexAI
SfiI SgfI SgrAI SnaBI SphI SrfI SspI StuI
SwaI XbaI XcmI XmnI