(Circular) (Six-base) MAPSORT of: pFN21AB5866.seq Check: 6777 from: 1 to: 6178 pFN21AB5866 6178 bp With 182 enzymes: SgfI * April 15, 2010 09:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3988 278 Size: 53 83 186 936 2452 2468 Fragments arranged by size: 2468 2452 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2808 3521 1937 Size: 871 713 4594 Fragments arranged by size: 4594 871 713 Acc65I G'GTAC_C Cuts at: 3499 3499 Size: 6178 AclI AA'CG_TT Cuts at: 1563 4305 4678 1563 Size: 2742 373 3063 Fragments arranged by size: 3063 2742 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2110 3202 4241 5625 5893 1632 Size: 478 1092 1039 1384 268 1917 Fragments arranged by size: 1917 1384 1092 1039 478 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5981 AflIII A'CryG_T Cuts at: 1203 2445 5077 6054 1203 Size: 1242 2632 977 1327 Fragments arranged by size: 2632 1327 1242 977 AgeI A'CCGG_T Cuts at: 5009 5009 Size: 6178 AhdI GACnn_n'nnGTC Cuts at: 4907 4907 Size: 6178 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6178 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6178 AlwNI CAG_nnn'CTG Cuts at: 3921 5493 3921 Size: 1572 4606 ApaLI G'TGCA_C Cuts at: 4235 5391 4235 Size: 1156 5022 ApoI r'AATT_y Cuts at: 1493 3487 3713 3865 5974 1493 Size: 1994 226 152 2109 1697 Fragments arranged by size: 2109 1994 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4732 160 Size: 872 3700 1606 Fragments arranged by size: 3700 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6178 AvaI C'yCGr_G Cuts at: 1870 1943 3503 1870 Size: 73 1560 4545 Fragments arranged by size: 4545 1560 73 BamHI G'GATC_C Cuts at: 2893 3508 3875 2893 Size: 615 367 5196 Fragments arranged by size: 5196 615 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3499 4954 618 Size: 325 217 627 1712 1455 1842 Fragments arranged by size: 1842 1712 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2224 3497 1391 Size: 833 1273 4072 Fragments arranged by size: 4072 1273 833 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2282 3291 928 Size: 971 383 1009 3815 Fragments arranged by size: 3815 1009 971 383 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4369 4403 1903 Size: 34 2432 34 3678 Fragments arranged by size: 3678 2432 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4070 5280 4070 Size: 1210 4968 BclI T'GATC_A Cuts at: 1548 1548 Size: 6178 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4789 136 Size: 107 122 71 631 605 3117 1525 Fragments arranged by size: 3117 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3111 6173 3111 Size: 3062 3116 BlpI GC'TnA_GC Cuts at: 3595 3595 Size: 6178 Bme1580I G_kGCm'C Cuts at: 1790 2134 4239 5395 1790 Size: 344 2105 1156 2573 Fragments arranged by size: 2573 2105 1156 344 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6178 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3355 3909 4867 Size: 416 53 83 399 24 1938 554 958 Cuts at: 4867 442 Size: 1753 Fragments arranged by size: 1938 1753 958 554 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6178 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2539 2539 Size: 6178 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2301 4838 1239 Size: 1062 2537 2579 Fragments arranged by size: 2579 2537 1062 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4303 5168 5466 4303 Size: 865 298 5015 Fragments arranged by size: 5015 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4841 882 Size: 617 3342 2219 Fragments arranged by size: 3342 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3225 3969 493 Size: 711 2021 744 2702 Fragments arranged by size: 2702 2021 744 711 BsaBI GATnn'nnATC Cuts at: 6172 6172 Size: 6178 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3985 4367 5002 275 Size: 53 83 186 936 2452 382 635 1451 Fragments arranged by size: 2452 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4611 5009 5283 5430 5841 4611 Size: 398 274 147 411 4948 Fragments arranged by size: 4948 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3042 3072 1178 Size: 30 165 30 1639 30 4284 Fragments arranged by size: 4284 1639 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2129 2416 2512 1186 Size: 943 287 96 4852 Fragments arranged by size: 4852 943 287 96 BseYI C'CCAG_C Cuts at: 2174 5381 5877 2174 Size: 3207 496 2475 Fragments arranged by size: 3207 2475 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5605 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3628 4389 5417 1540 Size: 275 186 1627 761 1028 2301 Fragments arranged by size: 2301 1627 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4239 4324 5395 4239 Size: 85 1071 5022 Fragments arranged by size: 5022 1071 85 BsmI GAATG_Cn' Cuts at: 3689 3782 3689 Size: 93 6085 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2134 2224 3497 4239 4324 5395 1391 Size: 399 344 90 1273 742 85 1071 2174 Fragments arranged by size: 2174 1273 1071 742 399 344 90 85 BspEI T'CCGG_A Cuts at: 5841 5841 Size: 6178 BspHI T'CATG_A Cuts at: 4067 5797 4067 Size: 1730 4448 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3533 844 Size: 2689 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4065 1394 Size: 429 2242 3507 Fragments arranged by size: 3507 2242 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4673 4847 65 Size: 1183 3425 174 1396 Fragments arranged by size: 3425 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3140 4822 5009 1700 Size: 110 1330 1682 187 2869 Fragments arranged by size: 2869 1682 1330 187 110 BsrGI T'GTAC_A Cuts at: 96 3085 96 Size: 2989 3189 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6178 BssSI C'ACGA_G Cuts at: 4238 5250 4238 Size: 1012 5166 BstAPI GCAn_nnn'nTGC Cuts at: 794 2682 794 Size: 1888 4290 BstBI TT'CG_AA Cuts at: 4982 4982 Size: 6178 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6178 BstYI r'GATC_y Cuts at: 1972 2893 3111 3259 3508 3875 4260 4277 5729 Size: 921 218 148 249 367 385 17 1452 Cuts at: 5729 5815 5827 6173 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 921 385 367 346 249 218 148 86 17 12 BstZ17I GTA'TAC Cuts at: 2809 2809 Size: 6178 Bsu36I CC'TnA_GG Cuts at: 2180 2180 Size: 6178 BtgI C'CryG_G Cuts at: 513 1065 2134 513 Size: 552 1069 4557 Fragments arranged by size: 4557 1069 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5439 Fragments arranged by size: 5439 683 56 BtsI GCAGTG_nn' Cuts at: 3694 4488 4508 3694 Size: 794 20 5364 Fragments arranged by size: 5364 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6178 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6143 DraI TTT'AAA Cuts at: 3482 3482 Size: 6178 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2789 5185 809 Size: 507 1473 2396 1802 Fragments arranged by size: 2396 1802 1473 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3625 4514 6143 Size: 54 1425 50 275 18 1795 889 1629 Cuts at: 6143 8 Size: 43 Fragments arranged by size: 1795 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3625 1537 Size: 275 1813 4090 Fragments arranged by size: 4090 1813 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2153 2160 2434 4108 1353 Size: 800 7 274 1674 3423 Fragments arranged by size: 3423 1674 800 274 7 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4765 5139 5285 387 Size: 1064 138 3176 374 146 1280 Fragments arranged by size: 3176 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2110 2301 3202 4241 4838 5625 5893 Size: 393 478 191 901 1039 597 787 268 Cuts at: 5893 1239 Size: 1524 Fragments arranged by size: 1524 1039 901 787 597 478 393 268 191 EcoO109I rG'GnC_Cy Cuts at: 3011 3459 3011 Size: 448 5730 EcoRI G'AATT_C Cuts at: 3487 3865 3487 Size: 378 5800 FspI TGC'GCA Cuts at: 4684 4684 Size: 6178 HaeII r_GCGC'y Cuts at: 1678 5325 1678 Size: 3647 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3149 3181 4819 4851 4893 Size: 32 135 32 1577 32 1638 32 42 Cuts at: 4893 4925 1373 Size: 32 2626 Fragments arranged by size: 2626 1638 1577 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2104 2875 3252 3522 3768 669 Size: 988 281 166 771 377 270 246 3079 Fragments arranged by size: 3079 988 771 377 281 270 246 166 HindIII A'AGCT_T Cuts at: 748 748 Size: 6178 HpaI GTT'AAC Cuts at: 3252 3768 3252 Size: 516 5662 KpnI G_GTAC'C Cuts at: 3503 3503 Size: 6178 MfeI C'AATT_G Cuts at: 3777 3777 Size: 6178 MluI A'CGCG_T Cuts at: 6054 6054 Size: 6178 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5292 5476 1305 Size: 3987 184 2007 Fragments arranged by size: 3987 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4356 Fragments arranged by size: 4356 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4136 4495 4654 518 Size: 648 2970 359 159 2042 Fragments arranged by size: 2970 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2240 3172 3594 4271 5419 5664 1606 Size: 634 932 422 677 1148 245 2120 Fragments arranged by size: 2120 1148 932 677 634 422 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5626 NdeI CA'TA_TG Cuts at: 387 387 Size: 6178 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6178 NotI GC'GGCC_GC Cuts at: 3625 3625 Size: 6178 NspI r_CATG'y Cuts at: 1132 1229 2449 3214 3536 5081 1132 Size: 97 1220 765 322 1545 2229 Fragments arranged by size: 2229 1545 1220 765 322 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6178 PciI A'CATG_T Cuts at: 2445 5077 2445 Size: 2632 3546 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6178 PfoI T'CCnGG_A Cuts at: 2607 2607 Size: 6178 PmeI GTTT'AAAC Cuts at: 3482 3482 Size: 6178 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4247 4279 5760 5792 4247 Size: 32 1481 32 4633 Fragments arranged by size: 4633 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3011 3011 Size: 6178 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6178 PsiI TTA'TAA Cuts at: 3748 3748 Size: 6178 PstI C_TGCA'G Cuts at: 830 3530 830 Size: 2700 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6178 PvuII CAG'CTG Cuts at: 2240 3172 2240 Size: 932 5246 SalI G'TCGA_C Cuts at: 1936 3520 1936 Size: 1584 4594 SanDI GG'GwC_CC Cuts at: 3011 3011 Size: 6178 SbfI CC_TGCA'GG Cuts at: 3530 3530 Size: 6178 ScaI AGT'ACT Cuts at: 2519 4426 6138 2519 Size: 1907 1712 2559 Fragments arranged by size: 2559 1907 1712 SexAI A'CCwGG_T Cuts at: 2959 2959 Size: 6178 SfcI C'TryA_G Cuts at: 826 1045 2325 3526 4661 5342 5533 826 Size: 219 1280 1201 1135 681 191 1471 Fragments arranged by size: 1471 1280 1201 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6178 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6178 SmaI CCC'GGG Cuts at: 1872 3505 1872 Size: 1633 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4282 5183 5445 820 Size: 197 926 2339 901 262 1553 Fragments arranged by size: 2339 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3969 493 Size: 3476 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6178 SphI G_CATG'C Cuts at: 1132 3536 1132 Size: 2404 3774 SspI AAT'ATT Cuts at: 5 52 2669 2701 2969 4102 5 Size: 47 2617 32 268 1133 2081 Fragments arranged by size: 2617 2081 1133 268 47 32 StuI AGG'CCT Cuts at: 1517 2185 1517 Size: 668 5510 StyI C'CwwG_G Cuts at: 513 1065 2095 3617 513 Size: 552 1030 1522 3074 Fragments arranged by size: 3074 1522 1030 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4205 4222 4375 4560 5005 325 Size: 1255 2625 17 153 185 445 1498 Fragments arranged by size: 2625 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2442 2517 3085 Size: 274 80 33 51 1443 465 75 568 Cuts at: 3085 3357 4424 6136 96 Size: 272 1067 1712 138 Fragments arranged by size: 1712 1443 1067 568 465 274 272 138 80 75 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2734 2766 2829 2861 6138 6170 2734 Size: 32 63 32 3277 32 2742 Fragments arranged by size: 3277 2742 63 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6178 XbaI T'CTAG_A Cuts at: 3514 3514 Size: 6178 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2967 2011 Size: 956 5222 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6178 XmaI C'CCGG_G Cuts at: 1870 3503 1870 Size: 1633 4545 XmnI GAAnn'nnTTC Cuts at: 4307 4307 Size: 6178 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3986 276 Size: 53 83 186 936 2452 2468 Fragments arranged by size: 2468 2452 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SapI SfiI SfoI SrfI SwaI