(Circular) (Six-base) MAPSORT of: pFN21AB5864.seq Check: 6478 from: 1 to: 6178 pFN21AB5864 6178 bp With 182 enzymes: SgfI * April 15, 2010 09:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2121 2121 Size: 6178 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3988 278 Size: 53 83 186 936 2452 2468 Fragments arranged by size: 2468 2452 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3521 1937 Size: 1584 4594 Acc65I G'GTAC_C Cuts at: 3499 3499 Size: 6178 AclI AA'CG_TT Cuts at: 1563 4305 4678 1563 Size: 2742 373 3063 Fragments arranged by size: 3063 2742 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2754 3233 4241 5625 5893 1632 Size: 1122 479 1008 1384 268 1917 Fragments arranged by size: 1917 1384 1122 1008 479 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5981 AflIII A'CryG_T Cuts at: 1203 5077 6054 1203 Size: 3874 977 1327 Fragments arranged by size: 3874 1327 977 AgeI A'CCGG_T Cuts at: 5009 5009 Size: 6178 AhdI GACnn_n'nnGTC Cuts at: 4907 4907 Size: 6178 AleI CACnn'nnGTG Cuts at: 1166 3414 1166 Size: 2248 3930 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6178 AlwNI CAG_nnn'CTG Cuts at: 2041 2050 3921 5493 2041 Size: 9 1871 1572 2726 Fragments arranged by size: 2726 1871 1572 9 ApaI G_GGCC'C Cuts at: 2077 2077 Size: 6178 ApaLI G'TGCA_C Cuts at: 4235 5391 4235 Size: 1156 5022 ApoI r'AATT_y Cuts at: 1493 3136 3487 3713 3865 5974 1493 Size: 1643 351 226 152 2109 1697 Fragments arranged by size: 2109 1697 1643 351 226 152 AseI AT'TA_AT Cuts at: 160 1032 4732 160 Size: 872 3700 1606 Fragments arranged by size: 3700 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6178 AvaI C'yCGr_G Cuts at: 1870 1943 2009 3290 3503 1870 Size: 73 66 1281 213 4545 Fragments arranged by size: 4545 1281 213 73 66 BamHI G'GATC_C Cuts at: 2433 3508 3875 2433 Size: 1075 367 4736 Fragments arranged by size: 4736 1075 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3499 4954 618 Size: 325 217 627 1712 1455 1842 Fragments arranged by size: 1842 1712 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2061 2077 2298 3497 1391 Size: 670 16 221 1199 4072 Fragments arranged by size: 4072 1199 670 221 16 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2524 928 Size: 971 625 4582 Fragments arranged by size: 4582 971 625 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2045 2079 4369 4403 1903 Size: 34 50 34 24 34 2290 34 3678 Fragments arranged by size: 3678 2290 50 34 34 34 34 24 BciVI GTATCCnnnnn_n' Cuts at: 4070 5280 4070 Size: 1210 4968 BclI T'GATC_A Cuts at: 1548 1548 Size: 6178 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2072 4789 136 Size: 107 122 71 631 605 400 2717 1525 Fragments arranged by size: 2717 1525 631 605 400 122 107 71 BglII A'GATC_T Cuts at: 2787 6173 2787 Size: 3386 2792 BlpI GC'TnA_GC Cuts at: 3595 3595 Size: 6178 Bme1580I G_kGCm'C Cuts at: 1790 2068 2077 2452 4239 5395 1790 Size: 278 9 375 1787 1156 2573 Fragments arranged by size: 2573 1787 1156 375 278 9 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6178 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3909 4867 442 Size: 416 53 83 399 24 2492 958 1753 Fragments arranged by size: 2492 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6178 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2506 2506 Size: 6178 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2099 3433 4838 1239 Size: 860 1334 1405 2579 Fragments arranged by size: 2579 1405 1334 860 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4303 5168 5466 4303 Size: 865 298 5015 Fragments arranged by size: 5015 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2198 2312 3399 4841 882 Size: 617 699 114 1087 1442 2219 Fragments arranged by size: 2219 1442 1087 699 617 114 BsaAI yAC'GTr Cuts at: 493 1204 3969 493 Size: 711 2765 2702 Fragments arranged by size: 2765 2702 711 BsaBI GATnn'nnATC Cuts at: 6172 6172 Size: 6178 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2457 3985 4367 5002 Size: 53 83 186 936 924 1528 382 635 Cuts at: 5002 275 Size: 1451 Fragments arranged by size: 1528 1451 936 924 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2878 4611 5009 5283 5430 5841 2878 Size: 1733 398 274 147 411 3215 Fragments arranged by size: 3215 1733 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2121 2151 2635 2665 1178 Size: 30 165 30 718 30 484 30 4691 Fragments arranged by size: 4691 718 484 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2368 1186 Size: 1182 4996 BseYI C'CCAG_C Cuts at: 2238 3397 5381 5877 2238 Size: 1159 1984 496 2539 Fragments arranged by size: 2539 1984 1159 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2486 1163 Size: 573 750 4855 Fragments arranged by size: 4855 750 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3628 4389 5417 1540 Size: 275 186 1627 761 1028 2301 Fragments arranged by size: 2301 1627 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2061 2085 4239 4324 5395 2061 Size: 24 2154 85 1071 2844 Fragments arranged by size: 2844 2154 1071 85 24 BsmI GAATG_Cn' Cuts at: 3457 3689 3782 3457 Size: 232 93 5853 Fragments arranged by size: 5853 232 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2638 2638 Size: 6178 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2061 2068 2077 2085 2298 2452 3497 Size: 399 271 7 9 8 213 154 1045 Cuts at: 3497 4239 4324 5395 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 1045 742 399 271 213 154 85 9 8 7 BspEI T'CCGG_A Cuts at: 5841 5841 Size: 6178 BspHI T'CATG_A Cuts at: 2950 4067 5797 2950 Size: 1117 1730 3331 Fragments arranged by size: 3331 1730 1117 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2121 3533 844 Size: 1277 1412 3489 Fragments arranged by size: 3489 1412 1277 BsrBI CCG'CTC Cuts at: 1394 1823 4065 1394 Size: 429 2242 3507 Fragments arranged by size: 3507 2242 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3153 3444 4673 4847 65 Size: 1183 1905 291 1229 174 1396 Fragments arranged by size: 1905 1396 1229 1183 291 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4822 5009 1700 Size: 110 3012 187 2869 Fragments arranged by size: 3012 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6178 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6178 BssSI C'ACGA_G Cuts at: 4238 5250 4238 Size: 1012 5166 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6178 BstBI TT'CG_AA Cuts at: 4982 4982 Size: 6178 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6178 BstYI r'GATC_y Cuts at: 1972 2433 2773 2787 3058 3169 3508 3875 4260 Size: 461 340 14 271 111 339 367 385 Cuts at: 4260 4277 5729 5815 5827 6173 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 461 385 367 346 340 339 271 111 86 17 14 12 BtgI C'CryG_G Cuts at: 513 1065 2068 2246 2452 513 Size: 552 1003 178 206 4239 Fragments arranged by size: 4239 1003 552 206 178 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5439 Fragments arranged by size: 5439 683 56 BtsI GCAGTG_nn' Cuts at: 2181 3694 4488 4508 2181 Size: 1513 794 20 3851 Fragments arranged by size: 3851 1513 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6178 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2647 2682 529 Size: 35 2083 35 4025 Fragments arranged by size: 4025 2083 35 35 DraI TTT'AAA Cuts at: 2688 3141 3482 2688 Size: 453 341 5384 Fragments arranged by size: 5384 453 341 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5185 809 Size: 507 3869 1802 Fragments arranged by size: 3869 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2052 2962 3625 Size: 54 1425 50 275 18 222 910 663 Cuts at: 3625 4514 6143 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 910 889 663 275 222 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3625 1537 Size: 275 1813 4090 Fragments arranged by size: 4090 1813 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2222 3212 4108 1353 Size: 869 990 896 3423 Fragments arranged by size: 3423 990 896 869 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4765 5139 5285 387 Size: 1064 138 3176 374 146 1280 Fragments arranged by size: 3176 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2059 2059 Size: 6178 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2099 2754 3233 3433 4241 4838 5625 Size: 393 467 655 479 200 808 597 787 Cuts at: 5625 5893 1239 Size: 268 1524 Fragments arranged by size: 1524 808 787 655 597 479 467 393 268 200 EcoNI CCTnn'n_nnAGG Cuts at: 3023 3023 Size: 6178 EcoO109I rG'GnC_Cy Cuts at: 2073 2074 2073 Size: 1 6177 EcoRI G'AATT_C Cuts at: 3487 3865 3487 Size: 378 5800 EcoRV GAT'ATC Cuts at: 2152 3031 2152 Size: 879 5299 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2932 2932 Size: 6178 FspI TGC'GCA Cuts at: 4684 4684 Size: 6178 HaeII r_GCGC'y Cuts at: 1678 2042 2473 5325 1678 Size: 364 431 2852 2531 Fragments arranged by size: 2852 2531 431 364 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2121 2153 2758 2790 2927 Size: 32 135 32 549 32 605 32 137 Cuts at: 2927 2959 4819 4851 4893 4925 1373 Size: 32 1860 32 42 32 2626 Fragments arranged by size: 2626 1860 605 549 137 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2580 3522 3768 669 Size: 988 281 642 942 246 3079 Fragments arranged by size: 3079 988 942 642 281 246 HindIII A'AGCT_T Cuts at: 748 2909 748 Size: 2161 4017 HpaI GTT'AAC Cuts at: 3768 3768 Size: 6178 KpnI G_GTAC'C Cuts at: 3503 3503 Size: 6178 MfeI C'AATT_G Cuts at: 3777 3777 Size: 6178 MluI A'CGCG_T Cuts at: 6054 6054 Size: 6178 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3119 5292 5476 1305 Size: 1814 2173 184 2007 Fragments arranged by size: 2173 2007 1814 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4356 Fragments arranged by size: 4356 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3365 3414 4136 4495 4654 518 Size: 648 2199 49 722 359 159 2042 Fragments arranged by size: 2199 2042 722 648 359 159 49 MspA1I CmG'CkG Cuts at: 1606 2047 2091 3594 4271 5419 5664 1606 Size: 441 44 1503 677 1148 245 2120 Fragments arranged by size: 2120 1503 1148 677 441 245 44 NcoI C'CATG_G Cuts at: 513 1065 2452 513 Size: 552 1387 4239 Fragments arranged by size: 4239 1387 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6178 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6178 NotI GC'GGCC_GC Cuts at: 3625 3625 Size: 6178 NspI r_CATG'y Cuts at: 1132 1229 3536 5081 1132 Size: 97 2307 1545 2229 Fragments arranged by size: 2307 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 3006 1348 Size: 1658 4520 PciI A'CATG_T Cuts at: 5077 5077 Size: 6178 PflMI CCAn_nnn'nTGG Cuts at: 1108 2971 3101 1108 Size: 1863 130 4185 Fragments arranged by size: 4185 1863 130 PfoI T'CCnGG_A Cuts at: 2436 2436 Size: 6178 PmeI GTTT'AAAC Cuts at: 3482 3482 Size: 6178 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4247 4279 5760 5792 4247 Size: 32 1481 32 4633 Fragments arranged by size: 4633 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6178 PsiI TTA'TAA Cuts at: 3125 3748 3125 Size: 623 5555 PspOMI G'GGCC_C Cuts at: 2073 2073 Size: 6178 PstI C_TGCA'G Cuts at: 830 2046 3530 830 Size: 1216 1484 3478 Fragments arranged by size: 3478 1484 1216 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6178 PvuII CAG'CTG Cuts at: 2047 2091 2047 Size: 44 6134 SacI G_AGCT'C Cuts at: 2061 2061 Size: 6178 SalI G'TCGA_C Cuts at: 1936 3520 1936 Size: 1584 4594 SbfI CC_TGCA'GG Cuts at: 3530 3530 Size: 6178 ScaI AGT'ACT Cuts at: 4426 6138 4426 Size: 1712 4466 SfcI C'TryA_G Cuts at: 826 1045 2042 3526 4661 5342 5533 826 Size: 219 997 1484 1135 681 191 1471 Fragments arranged by size: 1484 1471 1135 997 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6178 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6178 SmaI CCC'GGG Cuts at: 1872 2011 3505 1872 Size: 139 1494 4545 Fragments arranged by size: 4545 1494 139 SmlI C'TyrA_G Cuts at: 820 1017 1943 4282 5183 5445 820 Size: 197 926 2339 901 262 1553 Fragments arranged by size: 2339 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3969 493 Size: 3476 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6178 SphI G_CATG'C Cuts at: 1132 3536 1132 Size: 2404 3774 SspI AAT'ATT Cuts at: 5 52 4102 5 Size: 47 4050 2081 Fragments arranged by size: 4050 2081 47 StuI AGG'CCT Cuts at: 1517 2262 1517 Size: 745 5433 StyI C'CwwG_G Cuts at: 513 1065 2452 2751 3617 513 Size: 552 1387 299 866 3074 Fragments arranged by size: 3074 1387 866 552 299 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2438 4205 4222 4375 4560 5005 325 Size: 1255 858 1767 17 153 185 445 1498 Fragments arranged by size: 1767 1498 1255 858 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4424 6136 96 Size: 274 80 33 51 1443 2447 1712 138 Fragments arranged by size: 2447 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3235 3267 3443 3475 6138 6170 3235 Size: 32 176 32 2663 32 3243 Fragments arranged by size: 3243 2663 176 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6178 XbaI T'CTAG_A Cuts at: 3514 3514 Size: 6178 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3052 3325 3052 Size: 273 5905 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6178 XmaI C'CCGG_G Cuts at: 1870 2009 3503 1870 Size: 139 1494 4545 Fragments arranged by size: 4545 1494 139 XmnI GAAnn'nnTTC Cuts at: 4307 4307 Size: 6178 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3986 276 Size: 53 83 186 936 2452 2468 Fragments arranged by size: 2468 2452 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI