Restriction map for
pFN21AB7994
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# NO CUT ENZYMES ( 128 enzymes) #
##################################
AatII AccI AccIII AclI AflII
AflIII AhdI Aor51HI AscI AseI
AsnI Asp700I AspEI AviII AvrII
BbeI BbrPI BbsI BbvCI BcgI
BciVI BfaI BfrI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BseAI
BsgI BsiEI BsiWI BsmBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstXI BstZ17I CelII
ClaI CpoI DraI DraIII DrdI
DsaI EagI Eam1105I EarI EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRI
EcoRV FseI FspI HaeII HincII
HindII HindIII HpaI KasI Ksp632I
KspI MaeI MaeII MfeI MluI
MroI MspA1I MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
###################################
Acc65I 476 GGTACC G^GTACC
AocI 424 CCTGAGG CC^T[ACGT]AGG
ApaI 1282 GGGCCC GGGCC^C
Asp718I 476 GGTACC G^GTACC
AspI 451 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 38 CCCGGG C^[CT]CG[GA]G
AvaII 497 GGTCC G^G[AT]CC
BamHI 420 GGATCC G^GATCC
BanII 1282 GGGCCC G[GA]GC[CT]^C
BclI 359 TGATCA T^GATCA
BglI 537 GCCAGCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI 313 GAGACC GGTCTC(1/5)
BsoBI 38 CCCGGG C^[CT]CG[GA]G
BsrBI 75 GAGCGG CCGCTC(-3/-3)
BstAPI 787 GCAGTCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 420 GGATCC [GA]^GATC[CT]
Bsu36I 424 CCTGAGG CC^T[ACGT]AGG
CfoI 64 GCGC GCG^C
Eco81I 424 CCTGAGG CC^T[ACGT]AGG
EcoT22I 670 ATGCAT ATGCA^T
FauI 528 CCCGC CCCGC(4/6)
FbaI 359 TGATCA T^GATCA
HhaI 64 GCGC GCG^C
HinP1I 64 GCGC G^CGC
KpnI 476 GGTACC GGTAC^C
MaeIII 790 GTCAC ^GT[ACGT]AC
MflI 420 GGATCC [GA]^GATC[CT]
MseI 701 TTAA T^TAA
NsiI 670 ATGCAT ATGCA^T
NspI 1250 ACATGC [GA]CATG^[CT]
PflFI 451 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 981 GAGTC GAGTC(4/5)
PstI 303 CTGCAG CTGCA^G
SfcI 303 CTGCAG C^T[GA][CT]AG
SmaI 38 CCCGGG CCC^GGG
Tru9I 701 TTAA T^TAA
Tsp45I 790 GTCAC ^GT[GC]AC
Tth111I 451 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 420 GGATCC [GA]^GATC[CT]
XmaCI 38 CCCGGG C^CCGGG
XmaI 38 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccII 63 CGCG CG^CG
1256 CGCG
AcsI 334 AAATTC [GA]^AATT[CT]
748 GAATTT
AcyI 12 GGCGTC G[GA]^CG[CT]C
1329 GACGCC
AgeI 494 ACCGGT A^CCGGT
574 ACCGGT
Alw44I 240 GTGCAC G^TGCAC
691 GTGCAC
ApaLI 240 GTGCAC G^TGCAC
691 GTGCAC
ApoI 334 AAATTC [GA]^AATT[CT]
748 GAATTT
AspHI 240 GTGCAC G[AT]GC[AT]^C
691 GTGCAC
BalI 290 TGGCCA TGG^CCA
648 TGGCCA
BsaHI 12 GGCGTC G[GA]^CG[CT]C
1329 GACGCC
BsaWI 494 ACCGGT [AT]^CCGG[AT]
574 ACCGGT
BsiHKAI 240 GTGCAC G[AT]GC[AT]^C
691 GTGCAC
BsmAI 313 GAGAC GTCTC(1/5)
1032 GTCTC
BsmI 1181 GAATGC GAATGC(1/-1)
918 GCATTC
BsrFI 494 ACCGGT [GA]^CCGG[CT]
574 ACCGGT
BstUI 63 CGCG CG^CG
1256 CGCG
Cfr10I 494 ACCGGT [GA]^CCGG[CT]
574 ACCGGT
DraII 1281 GGGGCCC [GA]G^G[ACGT]CC[CT]
1282 GGGCCCT
EcoO109I 1281 GGGGCCC [GA]G^G[ACGT]CC[CT]
1282 GGGCCCT
EcoT14I 317 CCAAGG C^C[AT][AT]GG
685 CCAAGG
HgaI 1329 GACGC GACGC(5/10)
13 GCGTC
Hin1I 12 GGCGTC G[GA]^CG[CT]C
1329 GACGCC
MluNI 290 TGGCCA TGG^CCA
648 TGGCCA
MscI 290 TGGCCA TGG^CCA
648 TGGCCA
MvnI 63 CGCG CG^CG
1256 CGCG
PflMI 156 CCAGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
339 CCATGAGCTGG
PinAI 494 ACCGGT A^CCGGT
574 ACCGGT
StyI 317 CCAAGG C^C[AT][AT]GG
685 CCAAGG
TaqI 73 TCGA T^CGA
999 TCGA
TthHB8I 73 TCGA T^CGA
999 TCGA
Van91I 156 CCAGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
339 CCATGAGCTGG
XcmI 278 CCAGTGAGGAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
626 CCAACAATGAACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
BanI BbvI BcnI BmyI BpmI
BsaBI BsaJI BseRI BsiYI BslI
BsmFI Bsp1286I BsrDI BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII EaeI EcoRII Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI MamI MboI MboII MnlI
MslI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI SfaNI TfiI TseI
Tsp509I TspRI