(Circular) (Six-base) MAPSORT of: pFN21AB7994.seq Check: 1100 from: 1 to: 6046 pFN21AB7994 6046 bp With 182 enzymes: SgfI * April 15, 2010 09:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3856 278 Size: 53 83 186 936 2320 2468 Fragments arranged by size: 2468 2320 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3389 1937 Size: 1452 4594 Acc65I G'GTAC_C Cuts at: 2481 3367 2481 Size: 886 5160 AclI AA'CG_TT Cuts at: 1563 4173 4546 1563 Size: 2610 373 3063 Fragments arranged by size: 3063 2610 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2762 2769 4109 5493 5761 1632 Size: 1130 7 1340 1384 268 1917 Fragments arranged by size: 1917 1384 1340 1130 268 7 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5849 AflIII A'CryG_T Cuts at: 1203 4945 5922 1203 Size: 3742 977 1327 Fragments arranged by size: 3742 1327 977 AgeI A'CCGG_T Cuts at: 2499 2579 4877 2499 Size: 80 2298 3668 Fragments arranged by size: 3668 2298 80 AhdI GACnn_n'nnGTC Cuts at: 4775 4775 Size: 6046 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6046 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2946 1184 Size: 1762 4284 AlwNI CAG_nnn'CTG Cuts at: 2167 2798 3048 3097 3789 5361 2167 Size: 631 250 49 692 1572 2852 Fragments arranged by size: 2852 1572 692 631 250 49 ApaI G_GGCC'C Cuts at: 3291 3291 Size: 6046 ApaLI G'TGCA_C Cuts at: 2245 2696 4103 5259 2245 Size: 451 1407 1156 3032 Fragments arranged by size: 3032 1407 1156 451 ApoI r'AATT_y Cuts at: 1493 2339 2753 3355 3581 3733 5842 1493 Size: 846 414 602 226 152 2109 1697 Fragments arranged by size: 2109 1697 846 602 414 226 152 AseI AT'TA_AT Cuts at: 160 1032 4600 160 Size: 872 3568 1606 Fragments arranged by size: 3568 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6046 AvaI C'yCGr_G Cuts at: 1870 1943 2043 3371 1870 Size: 73 100 1328 4545 Fragments arranged by size: 4545 1328 100 73 BamHI G'GATC_C Cuts at: 2425 3376 3743 2425 Size: 951 367 4728 Fragments arranged by size: 4728 951 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2354 2481 3110 3367 4822 Size: 325 217 627 567 127 629 257 1455 Cuts at: 4822 618 Size: 1842 Fragments arranged by size: 1842 1455 629 627 567 325 257 217 127 BanII G_rGCy'C Cuts at: 1391 3291 3365 1391 Size: 1900 74 4072 Fragments arranged by size: 4072 1900 74 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5075 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4237 4271 1903 Size: 34 2300 34 3678 Fragments arranged by size: 3678 2300 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3938 5148 3938 Size: 1210 4836 BclI T'GATC_A Cuts at: 1548 2364 1548 Size: 816 5230 BfrBI ATG'CAT Cuts at: 2677 2677 Size: 6046 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2548 4657 136 Size: 107 122 71 631 605 876 2109 1525 Fragments arranged by size: 2109 1525 876 631 605 122 107 71 BglII A'GATC_T Cuts at: 6041 6041 Size: 6046 BlpI GC'TnA_GC Cuts at: 3463 3463 Size: 6046 Bme1580I G_kGCm'C Cuts at: 1790 2161 2249 2357 2700 3291 4107 5263 1790 Size: 371 88 108 343 591 816 1156 2573 Fragments arranged by size: 2573 1156 816 591 371 343 108 88 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6046 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2277 3191 3777 Size: 416 53 83 399 24 860 914 586 Cuts at: 3777 4735 442 Size: 958 1753 Fragments arranged by size: 1753 958 914 860 586 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6046 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2150 2336 2857 4706 1239 Size: 911 186 521 1849 2579 Fragments arranged by size: 2579 1849 911 521 186 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4171 5036 5334 4171 Size: 865 298 4883 Fragments arranged by size: 4883 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2312 4709 882 Size: 617 813 2397 2219 Fragments arranged by size: 2397 2219 813 617 BsaAI yAC'GTr Cuts at: 493 1204 3837 493 Size: 711 2633 2702 Fragments arranged by size: 2702 2633 711 BsaBI GATnn'nnATC Cuts at: 2366 2456 2841 6040 2366 Size: 90 385 3199 2372 Fragments arranged by size: 3199 2372 385 90 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2018 3335 3853 4235 Size: 53 83 186 936 485 1317 518 382 Cuts at: 4235 4870 275 Size: 635 1451 Fragments arranged by size: 1451 1317 936 635 518 485 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2499 2579 4479 4877 5151 5298 5709 2499 Size: 80 1900 398 274 147 411 2836 Fragments arranged by size: 2836 1900 411 398 274 147 80 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2042 2072 2135 2165 2489 Size: 30 165 30 639 30 63 30 324 Cuts at: 2489 2519 1178 Size: 30 4705 Fragments arranged by size: 4705 639 324 165 63 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2029 2224 2227 2973 1186 Size: 843 195 3 746 4259 Fragments arranged by size: 4259 843 746 195 3 BseYI C'CCAG_C Cuts at: 2160 2350 5249 5745 2160 Size: 190 2899 496 2461 Fragments arranged by size: 2899 2461 496 190 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5473 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3496 4257 5285 1540 Size: 275 186 1495 761 1028 2301 Fragments arranged by size: 2301 1495 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2249 2700 4107 4192 5263 2249 Size: 451 1407 85 1071 3032 Fragments arranged by size: 3032 1407 1071 451 85 BsmI GAATG_Cn' Cuts at: 2923 3192 3557 3650 2923 Size: 269 365 93 5319 Fragments arranged by size: 5319 365 269 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2161 2249 2357 2700 3291 3365 4107 Size: 399 371 88 108 343 591 74 742 Cuts at: 4107 4192 5263 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 591 399 371 343 108 88 85 74 BspEI T'CCGG_A Cuts at: 5709 5709 Size: 6046 BspHI T'CATG_A Cuts at: 3935 5665 3935 Size: 1730 4316 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3401 844 Size: 2557 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2082 3933 1394 Size: 429 259 1851 3507 Fragments arranged by size: 3507 1851 429 259 BsrDI GCAATG_nn' Cuts at: 65 1248 2061 2729 3207 4541 4715 65 Size: 1183 813 668 478 1334 174 1396 Fragments arranged by size: 1396 1334 1183 813 668 478 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2499 2579 4690 4877 1700 Size: 110 689 80 2111 187 2869 Fragments arranged by size: 2869 2111 689 187 110 80 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6046 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6046 BssSI C'ACGA_G Cuts at: 4106 5118 4106 Size: 1012 5034 BstAPI GCAn_nnn'nTGC Cuts at: 794 2798 794 Size: 2004 4042 BstBI TT'CG_AA Cuts at: 4850 4850 Size: 6046 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6046 BstYI r'GATC_y Cuts at: 1972 2425 3376 3743 4128 4145 5597 5683 5695 Size: 453 951 367 385 17 1452 86 12 Cuts at: 5695 6041 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 951 453 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2430 2430 Size: 6046 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4555 Fragments arranged by size: 4555 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5307 Fragments arranged by size: 5307 683 56 BtsI GCAGTG_nn' Cuts at: 2221 2796 3562 4356 4376 2221 Size: 575 766 794 20 3891 Fragments arranged by size: 3891 794 766 575 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6046 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6011 DraI TTT'AAA Cuts at: 3350 3350 Size: 6046 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5053 809 Size: 507 3737 1802 Fragments arranged by size: 3737 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2295 2653 3204 Size: 54 1425 50 275 18 465 358 551 Cuts at: 3204 3493 4382 6011 8 Size: 289 889 1629 43 Fragments arranged by size: 1629 1425 889 551 465 358 289 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3493 1537 Size: 275 1681 4090 Fragments arranged by size: 4090 1681 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3976 1353 Size: 2623 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4633 5007 5153 387 Size: 1064 138 3044 374 146 1280 Fragments arranged by size: 3044 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2150 2336 2762 2769 2857 4109 4706 Size: 393 518 186 426 7 88 1252 597 Cuts at: 4706 5493 5761 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1252 787 597 518 426 393 268 186 88 7 EcoO109I rG'GnC_Cy Cuts at: 3287 3288 3287 Size: 1 6045 EcoRI G'AATT_C Cuts at: 3355 3733 3355 Size: 378 5668 FspI TGC'GCA Cuts at: 4552 4552 Size: 6046 HaeII r_GCGC'y Cuts at: 1678 5193 1678 Size: 3515 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2135 2167 3020 3052 4687 Size: 32 135 32 563 32 853 32 1635 Cuts at: 4687 4719 4761 4793 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1635 853 563 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3390 3636 669 Size: 988 281 1452 246 3079 Fragments arranged by size: 3079 1452 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6046 HpaI GTT'AAC Cuts at: 3636 3636 Size: 6046 KpnI G_GTAC'C Cuts at: 2485 3371 2485 Size: 886 5160 MfeI C'AATT_G Cuts at: 3645 3645 Size: 6046 MluI A'CGCG_T Cuts at: 5922 5922 Size: 6046 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2420 5160 5344 1305 Size: 1115 2740 184 2007 Fragments arranged by size: 2740 2007 1115 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2297 2655 10 Size: 54 1425 343 465 358 3401 Fragments arranged by size: 3401 1425 465 358 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2360 2405 2928 4004 4363 4522 518 Size: 648 1194 45 523 1076 359 159 2042 Fragments arranged by size: 2042 1194 1076 648 523 359 159 45 MspA1I CmG'CkG Cuts at: 1606 3462 4139 5287 5532 1606 Size: 1856 677 1148 245 2120 Fragments arranged by size: 2120 1856 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4555 Fragments arranged by size: 4555 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6046 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6046 NotI GC'GGCC_GC Cuts at: 3493 3493 Size: 6046 NsiI A_TGCA'T Cuts at: 2679 2679 Size: 6046 NspI r_CATG'y Cuts at: 1132 1229 3259 3404 4949 1132 Size: 97 2030 145 1545 2229 Fragments arranged by size: 2229 2030 1545 145 97 PasI CC'CwG_GG Cuts at: 1348 2167 1348 Size: 819 5227 PciI A'CATG_T Cuts at: 4945 4945 Size: 6046 PflMI CCAn_nnn'nTGG Cuts at: 1108 2167 2350 1108 Size: 1059 183 4804 Fragments arranged by size: 4804 1059 183 PfoI T'CCnGG_A Cuts at: 2862 2862 Size: 6046 PmeI GTTT'AAAC Cuts at: 3350 3350 Size: 6046 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4115 4147 5628 5660 4115 Size: 32 1481 32 4501 Fragments arranged by size: 4501 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6046 PsiI TTA'TAA Cuts at: 3616 3616 Size: 6046 PspOMI G'GGCC_C Cuts at: 3287 3287 Size: 6046 PstI C_TGCA'G Cuts at: 830 2312 3398 830 Size: 1482 1086 3478 Fragments arranged by size: 3478 1482 1086 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6046 SalI G'TCGA_C Cuts at: 1936 3388 1936 Size: 1452 4594 SbfI CC_TGCA'GG Cuts at: 3398 3398 Size: 6046 ScaI AGT'ACT Cuts at: 4294 6006 4294 Size: 1712 4334 SfcI C'TryA_G Cuts at: 826 1045 2308 3394 4529 5210 5401 826 Size: 219 1263 1086 1135 681 191 1471 Fragments arranged by size: 1471 1263 1135 1086 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6046 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6046 SmaI CCC'GGG Cuts at: 1872 2045 3373 1872 Size: 173 1328 4545 Fragments arranged by size: 4545 1328 173 SmlI C'TyrA_G Cuts at: 820 1017 1943 4150 5051 5313 820 Size: 197 926 2207 901 262 1553 Fragments arranged by size: 2207 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3837 493 Size: 3344 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6046 SphI G_CATG'C Cuts at: 1132 3404 1132 Size: 2272 3774 SspI AAT'ATT Cuts at: 5 52 3970 5 Size: 47 3918 2081 Fragments arranged by size: 3918 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6046 StyI C'CwwG_G Cuts at: 513 1065 2004 2322 2690 3485 513 Size: 552 939 318 368 795 3074 Fragments arranged by size: 3074 939 795 552 368 318 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2159 2956 3102 3151 4073 4090 4243 Size: 1255 579 797 146 49 922 17 153 Cuts at: 4243 4428 4873 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 922 797 579 445 185 153 146 49 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2439 4292 6004 Size: 274 80 33 51 1443 462 1853 1712 Cuts at: 6004 96 Size: 138 Fragments arranged by size: 1853 1712 1443 462 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2489 2521 3077 3109 6006 6038 2489 Size: 32 556 32 2897 32 2497 Fragments arranged by size: 2897 2497 556 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2459 1316 Size: 1143 4903 XbaI T'CTAG_A Cuts at: 3382 3382 Size: 6046 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2290 2638 2290 Size: 348 5698 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6046 XmaI C'CCGG_G Cuts at: 1870 2043 3371 1870 Size: 173 1328 4545 Fragments arranged by size: 4545 1328 173 XmnI GAAnn'nnTTC Cuts at: 4175 4175 Size: 6046 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3854 276 Size: 53 83 186 936 2320 2468 Fragments arranged by size: 2468 2320 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI