Restriction map for
pFN21AB8490
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# NO CUT ENZYMES ( 96 enzymes) #
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Acc65I AccIII AclI AflII AgeI
AhdI Alw44I ApaLI AscI AseI
AsnI Asp718I AspEI AvrII BalI
BamHI BbeI BbrPI BclI BfrI
BglI BlnI BsaAI BsaI BseAI
BsiWI BsmI BspDI BspEI BspHI
BspLU11I BssHII Bst1107I BstBI BstEII
BstPI BstZ17I ClaI CpoI DraI
DrdI Eam1105I EarI EcoNI EcoO65I
EcoRV FbaI FseI HincII HindII
HindIII HpaI KasI KpnI Ksp632I
MfeI MluI MluNI MroI MscI
MseI MunI NarI NheI NotI
NruI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RcaI RsrII SalI SapI
ScaI SfiI SfoI SfuI SmaI
SnaBI SpeI SspI StuI SwaI
Tru9I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AatII 2395 GACGTC GACGT^C
AccI 2398 GTCTAC GT^[AC][GT]AC
AcyI 2395 GACGTC G[GA]^CG[CT]C
AflIII 518 ACACGT A^C[GA][CT]GT
Aor51HI 1976 AGCGCT AGC^GCT
Asp700I 359 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1455 GACGCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 384 TGCGCA TGC^GCA
BcgI 703 GCAGCAGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 2543 AGATCT A^GATCT
BsaBI 573 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 2395 GACGTC G[GA]^CG[CT]C
BsaWI 2051 ACCGGA [AT]^CCGG[AT]
BsiEI 209 CGGCCG CG[GA][CT]^CG
BsmBI 1453 GAGACG CGTCTC(1/5)
Bsp1407I 140 TGTACA T^GTACA
BsrGI 140 TGTACA T^GTACA
BstXI 2024 CCAAACGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 2543 AGATCT [GA]^GATC[CT]
DraIII 2376 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 209 CGGCCG C^GGCCG
EclXI 209 CGGCCG C^GGCCG
Eco47III 1976 AGCGCT AGC^GCT
Eco52I 209 CGGCCG C^GGCCG
EcoT22I 2323 ATGCAT ATGCA^T
FspI 384 TGCGCA TGC^GCA
Hin1I 2395 GACGTC G[GA]^CG[CT]C
KspI 269 CCGCGG CCGC^GG
MamI 573 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 2543 AGATCT [GA]^GATC[CT]
NcoI 1988 CCATGG C^CATGG
NdeI 2326 CATATG CA^TATG
NsiI 2323 ATGCAT ATGCA^T
PflFI 1455 GACGCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 1766 GACGCTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 269 CCGCGG CCGC^GG
SbfI 826 CCTGCAGG CCTGCA^GG
SexAI 2336 ACCTGGT A^CC[AT]GGT
SgrAI 2284 CGCCGGCG C[GA]^CCGG[CT]G
SphI 2561 GCATGC GCATG^C
Sse8387I 826 CCTGCAGG CCTGCA^GG
SspBI 140 TGTACA T^GTACA
Tth111I 1455 GACGCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 2543 AGATCT [GA]^GATC[CT]
XmnI 359 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 28 enzymes) #
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AccII 270 CGCG CG^CG
1457 CGCG
AcsI 2404 GAATTC [GA]^AATT[CT]
2446 GAATTC
AocI 482 CCTCAGG CC^T[ACGT]AGG
1613 CCTCAGG
ApoI 2404 GAATTC [GA]^AATT[CT]
2446 GAATTC
AvaI 711 CTCGGG C^[CT]CG[GA]G
1309 CCCGAG
BanI 832 GGTGCC G^G[CT][GA]CC
1428 GGCACC
BbvCI 291 CCTCAGC CCTCAGC(-5/-2)
1593 CCTCAGC
BciVI 2133 GGATAC GTATCC(6/5)
343 GTATCC
BfaI 32 CTAG C^TAG
1192 CTAG
BsoBI 711 CTCGGG C^[CT]CG[GA]G
1309 CCCGAG
BsrBI 212 CCGCTC CCGCTC(-3/-3)
2412 CCGCTC
BsrDI 966 CATTGC GCAATG(2/0)
374 GCAATG
BssSI 1524 CACGAG CACGAG(-5/-1)
1555 CACGAG
BstUI 270 CGCG CG^CG
1457 CGCG
Bsu36I 482 CCTCAGG CC^T[ACGT]AGG
1613 CCTCAGG
Eco81I 482 CCTCAGG CC^T[ACGT]AGG
1613 CCTCAGG
EcoRI 2404 GAATTC G^AATTC
2446 GAATTC
EcoT14I 1988 CCATGG C^C[AT][AT]GG
2129 CCAAGG
MaeI 32 CTAG C^TAG
1192 CTAG
MvnI 270 CGCG CG^CG
1457 CGCG
NaeI 1645 GCCGGC GCC^GGC
2285 GCCGGC
NgoMIV 1645 GCCGGC G^CCGGC
2285 GCCGGC
PflMI 309 CCAGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2375 CCACCAAGTGG
PpuMI 2303 GGGACCT [GA]G^G[AT]CC[CT]
2333 GGGACCT
PvuII 985 CAGCTG CAG^CTG
1355 CAGCTG
StyI 1988 CCATGG C^C[AT][AT]GG
2129 CCAAGG
TfiI 1788 GAATC G^A[AT]TC
1891 GAATC
Van91I 309 CCAGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2375 CCACCAAGTGG
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# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
ApaI AspHI AvaII BanII BbsI
BbvI BcnI BlpI BmyI BpmI
Bpu1102I BpuAI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrFI BsrI BssKI
BstAPI BstF5I BstNI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EcoO109I EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI MaeII MaeIII
MboI MboII MnlI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PstI
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI TaqI TseI
Tsp45I Tsp509I TspRI TthHB8I