(Circular) (Six-base) MAPSORT of: pFN21AB8490.seq Check: 5283 from: 1 to: 7282 pFN21AB8490 7282 bp With 182 enzymes: SgfI * April 15, 2010 09:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2167 2825 3042 2167 Size: 658 217 6407 Fragments arranged by size: 6407 658 217 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4404 5092 278 Size: 53 83 186 936 2868 688 2468 Fragments arranged by size: 2868 2468 936 688 186 83 53 AccI GT'mk_AC Cuts at: 1937 4404 4625 1937 Size: 2467 221 4594 Fragments arranged by size: 4594 2467 221 Acc65I G'GTAC_C Cuts at: 4603 4603 Size: 7282 AclI AA'CG_TT Cuts at: 1563 5409 5782 1563 Size: 3846 373 3063 Fragments arranged by size: 3846 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2355 3342 4565 5345 6729 6997 1632 Size: 723 987 1223 780 1384 268 1917 Fragments arranged by size: 1917 1384 1223 987 780 723 268 AfeI AGC'GCT Cuts at: 3983 3983 Size: 7282 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7085 AflIII A'CryG_T Cuts at: 1203 2523 6181 7158 1203 Size: 1320 3658 977 1327 Fragments arranged by size: 3658 1327 1320 977 AgeI A'CCGG_T Cuts at: 6113 6113 Size: 7282 AhdI GACnn_n'nnGTC Cuts at: 6011 6011 Size: 7282 AleI CACnn'nnGTG Cuts at: 1166 3226 1166 Size: 2060 5222 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4583 1184 Size: 3399 3883 AlwNI CAG_nnn'CTG Cuts at: 2046 2088 2194 2227 2320 2788 4082 4163 5025 Size: 42 106 33 93 468 1294 81 862 Cuts at: 5025 6597 2046 Size: 1572 2731 Fragments arranged by size: 2731 1572 1294 862 468 106 93 81 42 33 ApaI G_GGCC'C Cuts at: 2133 2464 2723 4539 2133 Size: 331 259 1816 4876 Fragments arranged by size: 4876 1816 331 259 ApaLI G'TGCA_C Cuts at: 5339 6495 5339 Size: 1156 6126 ApoI r'AATT_y Cuts at: 1493 4409 4451 4591 4817 4969 7078 1493 Size: 2916 42 140 226 152 2109 1697 Fragments arranged by size: 2916 2109 1697 226 152 140 42 AseI AT'TA_AT Cuts at: 160 1032 5836 160 Size: 872 4804 1606 Fragments arranged by size: 4804 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7282 AvaI C'yCGr_G Cuts at: 1870 1943 2716 3314 4607 1870 Size: 73 773 598 1293 4545 Fragments arranged by size: 4545 1293 773 598 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2546 2579 2546 Size: 33 7249 BamHI G'GATC_C Cuts at: 4612 4979 4612 Size: 367 6915 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2837 3433 4603 6058 618 Size: 325 217 627 1050 596 1170 1455 1842 Fragments arranged by size: 1842 1455 1170 1050 627 596 325 217 BanII G_rGCy'C Cuts at: 1391 2133 2464 2628 2723 2934 3126 3536 3885 Size: 742 331 164 95 211 192 410 349 Cuts at: 3885 3939 4320 4380 4539 4601 1391 Size: 54 381 60 159 62 4072 Fragments arranged by size: 4072 742 410 381 349 331 211 192 164 159 95 62 60 54 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2520 4218 4404 928 Size: 971 621 1698 186 3806 Fragments arranged by size: 3806 1698 971 621 186 BbvCI CC'TCA_GC Cuts at: 2297 3599 2297 Size: 1302 5980 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2697 2731 5473 5507 1903 Size: 34 760 34 2742 34 3678 Fragments arranged by size: 3678 2742 760 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2359 4132 5174 6384 2359 Size: 1773 1042 1210 3257 Fragments arranged by size: 3257 1773 1210 1042 BclI T'GATC_A Cuts at: 1548 1548 Size: 7282 BfrBI ATG'CAT Cuts at: 4330 4330 Size: 7282 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5893 136 Size: 107 122 71 631 605 4221 1525 Fragments arranged by size: 4221 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4548 7277 4548 Size: 2729 4553 BlpI GC'TnA_GC Cuts at: 2499 2933 2988 3120 4699 2499 Size: 434 55 132 1579 5082 Fragments arranged by size: 5082 1579 434 132 55 Bme1580I G_kGCm'C Cuts at: 1790 2133 2464 2575 2723 3327 3399 4170 4462 Size: 343 331 111 148 604 72 771 292 Cuts at: 4462 4539 5343 6499 1790 Size: 77 804 1156 2573 Fragments arranged by size: 2573 1156 804 771 604 343 331 292 148 111 77 72 BmgBI CAC'GTC Cuts at: 1318 2526 1318 Size: 1208 6074 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2398 3303 5013 Size: 416 53 83 399 24 981 905 1710 Cuts at: 5013 5971 442 Size: 958 1753 Fragments arranged by size: 1753 1710 981 958 905 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7282 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2267 2297 3149 3209 3625 4201 4334 5942 Size: 1028 30 852 60 416 576 133 1608 Cuts at: 5942 1239 Size: 2579 Fragments arranged by size: 2579 1608 1028 852 576 416 133 60 30 Bpu10I CC'TnA_GC Cuts at: 2297 2622 2820 3599 2297 Size: 325 198 779 5980 Fragments arranged by size: 5980 779 325 198 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3782 3911 4222 4478 4556 5407 6272 6570 3782 Size: 129 311 256 78 851 865 298 4494 Fragments arranged by size: 4494 865 851 311 298 256 129 78 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5945 882 Size: 617 4446 2219 Fragments arranged by size: 4446 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 5073 493 Size: 711 3869 2702 Fragments arranged by size: 3869 2702 711 BsaBI GATnn'nnATC Cuts at: 2582 7276 2582 Size: 4694 2588 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4401 5089 5471 6106 Size: 53 83 186 936 2868 688 382 635 Cuts at: 6106 275 Size: 1451 Fragments arranged by size: 2868 1451 936 688 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4056 5715 6113 6387 6534 6945 4056 Size: 1659 398 274 147 411 4393 Fragments arranged by size: 4393 1659 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2991 3021 3623 3653 1178 Size: 30 165 30 1588 30 602 30 4807 Fragments arranged by size: 4807 1588 602 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2441 2999 3893 3920 1186 Size: 1255 558 894 27 4548 Fragments arranged by size: 4548 1255 894 558 27 BseYI C'CCAG_C Cuts at: 2207 2247 2307 2434 2445 2533 2747 2797 2927 Size: 40 60 127 11 88 214 50 130 Cuts at: 2927 3093 3132 3170 3399 3553 4531 6485 6981 Size: 166 39 38 229 154 978 1954 496 Cuts at: 6981 2207 Size: 2508 Fragments arranged by size: 2508 1954 978 496 229 214 166 154 130 127 88 60 50 40 39 38 11 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2180 2195 2636 3230 1163 Size: 573 444 15 441 594 5215 Fragments arranged by size: 5215 594 573 444 441 15 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2217 4732 5493 6521 1540 Size: 275 186 216 2515 761 1028 2301 Fragments arranged by size: 2515 2301 1028 761 275 216 186 BsiHKAI G_wGCw'C Cuts at: 2514 2628 3114 3916 3939 4320 5343 5428 6499 Size: 114 486 802 23 381 1023 85 1071 Cuts at: 6499 2514 Size: 3297 Fragments arranged by size: 3297 1071 1023 802 486 381 114 85 23 BsmI GAATG_Cn' Cuts at: 4793 4886 4793 Size: 93 7189 BsmBI CGTCTCn'nnnn_ Cuts at: 3452 3452 Size: 7282 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2133 2464 2514 2575 2628 2723 2934 Size: 399 343 331 50 61 53 95 211 Cuts at: 2934 3114 3126 3327 3399 3536 3885 3916 3939 Size: 180 12 201 72 137 349 31 23 Cuts at: 3939 4170 4320 4380 4462 4539 4601 5343 5428 Size: 231 150 60 82 77 62 742 85 Cuts at: 5428 6499 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 349 343 331 231 211 201 180 150 137 95 85 82 77 72 62 61 60 53 50 31 23 12 BspEI T'CCGG_A Cuts at: 6945 6945 Size: 7282 BspHI T'CATG_A Cuts at: 5171 6901 5171 Size: 1730 5552 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2167 2235 2825 3042 3252 3333 4637 844 Size: 1323 68 590 217 210 81 1304 3489 Fragments arranged by size: 3489 1323 1304 590 217 210 81 68 BsrBI CCG'CTC Cuts at: 1394 1823 2219 4419 5169 1394 Size: 429 396 2200 750 3507 Fragments arranged by size: 3507 2200 750 429 396 BsrDI GCAATG_nn' Cuts at: 65 1248 2386 2970 5777 5951 65 Size: 1183 1138 584 2807 174 1396 Fragments arranged by size: 2807 1396 1183 1138 584 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2163 3650 4290 5926 6113 1700 Size: 110 353 1487 640 1636 187 2869 Fragments arranged by size: 2869 1636 1487 640 353 187 110 BsrGI T'GTAC_A Cuts at: 96 2145 96 Size: 2049 5233 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7282 BssSI C'ACGA_G Cuts at: 3529 3560 5342 6354 3529 Size: 31 1782 1012 4457 Fragments arranged by size: 4457 1782 1012 31 BstAPI GCAn_nnn'nTGC Cuts at: 794 2088 2173 2194 2263 2509 4082 4483 794 Size: 1294 85 21 69 246 1573 401 3593 Fragments arranged by size: 3593 1573 1294 401 246 85 69 21 BstBI TT'CG_AA Cuts at: 6086 6086 Size: 7282 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7282 BstXI CCAn_nnnn'nTGG Cuts at: 4036 4036 Size: 7282 BstYI r'GATC_y Cuts at: 1972 4548 4612 4979 5364 5381 6833 6919 6931 Size: 2576 64 367 385 17 1452 86 12 Cuts at: 6931 7277 1972 Size: 346 1977 Fragments arranged by size: 2576 1977 1452 385 367 346 86 64 17 12 Bsu36I CC'TnA_GG Cuts at: 2488 3619 2488 Size: 1131 6151 BtgI C'CryG_G Cuts at: 513 1065 2004 2274 2595 3993 513 Size: 552 939 270 321 1398 3802 Fragments arranged by size: 3802 1398 939 552 321 270 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2479 3631 487 Size: 683 56 1253 1152 4138 Fragments arranged by size: 4138 1253 1152 683 56 BtsI GCAGTG_nn' Cuts at: 2047 2089 2110 2905 3234 3275 3410 3498 3654 Size: 42 21 795 329 41 135 88 156 Cuts at: 3654 4798 5592 5612 2047 Size: 1144 794 20 3717 Fragments arranged by size: 3717 1144 795 794 329 156 135 88 42 41 21 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7282 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7247 DraI TTT'AAA Cuts at: 4586 4586 Size: 7282 DraIII CAC_nnn'GTG Cuts at: 4386 4386 Size: 7282 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6289 809 Size: 507 4973 1802 Fragments arranged by size: 4973 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2116 2214 2598 Size: 54 1425 50 275 18 286 98 384 Cuts at: 2598 3943 4729 5618 7247 8 Size: 1345 786 889 1629 43 Fragments arranged by size: 1629 1425 1345 889 786 384 286 275 98 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2214 4729 1537 Size: 275 402 2515 4090 Fragments arranged by size: 4090 2515 402 275 EarI CTCTTCn'nnn_ Cuts at: 1353 5212 1353 Size: 3859 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3767 5869 6243 6389 387 Size: 1064 138 2178 2102 374 146 1280 Fragments arranged by size: 2178 2102 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2626 3937 4318 2626 Size: 1311 381 5590 Fragments arranged by size: 5590 1311 381 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2267 2297 2355 3149 3209 3342 3625 Size: 393 635 30 58 794 60 133 283 Cuts at: 3625 4201 4334 4565 5345 5942 6729 6997 1239 Size: 576 133 231 780 597 787 268 1524 Fragments arranged by size: 1524 794 787 780 635 597 576 393 283 268 231 133 133 60 58 30 EcoO109I rG'GnC_Cy Cuts at: 2130 2191 2395 2720 4309 4339 4535 2130 Size: 61 204 325 1589 30 196 4877 Fragments arranged by size: 4877 1589 325 204 196 61 30 EcoRI G'AATT_C Cuts at: 4409 4451 4591 4969 4409 Size: 42 140 378 6722 Fragments arranged by size: 6722 378 140 42 FspI TGC'GCA Cuts at: 2391 5788 2391 Size: 3397 3885 HaeII r_GCGC'y Cuts at: 1678 2282 2307 3985 4518 6429 1678 Size: 604 25 1678 533 1911 2531 Fragments arranged by size: 2531 1911 1678 604 533 25 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3449 3481 5923 5955 5997 Size: 32 135 32 1877 32 2442 32 42 Cuts at: 5997 6029 1373 Size: 32 2626 Fragments arranged by size: 2626 2442 1877 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4626 4872 669 Size: 988 281 2688 246 3079 Fragments arranged by size: 3079 2688 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7282 HpaI GTT'AAC Cuts at: 4872 4872 Size: 7282 KpnI G_GTAC'C Cuts at: 4607 4607 Size: 7282 MfeI C'AATT_G Cuts at: 4881 4881 Size: 7282 MluI A'CGCG_T Cuts at: 7158 7158 Size: 7282 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6396 6580 1305 Size: 5091 184 2007 Fragments arranged by size: 5091 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5460 Fragments arranged by size: 5460 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3226 3503 4331 4565 5240 5599 5758 Size: 648 2060 277 828 234 675 359 159 Cuts at: 5758 518 Size: 2042 Fragments arranged by size: 2060 2042 828 675 648 359 277 234 159 MspA1I CmG'CkG Cuts at: 1606 2067 2276 2705 2992 3362 4306 4698 5375 Size: 461 209 429 287 370 944 392 677 Cuts at: 5375 6523 6768 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 944 677 461 429 392 370 287 245 209 NaeI GCC'GGC Cuts at: 3652 4292 3652 Size: 640 6642 NcoI C'CATG_G Cuts at: 513 1065 2004 3993 513 Size: 552 939 1989 3802 Fragments arranged by size: 3802 1989 939 552 NdeI CA'TA_TG Cuts at: 387 4332 387 Size: 3945 3337 NgoMIV G'CCGG_C Cuts at: 3650 4290 3650 Size: 640 6642 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7282 NotI GC'GGCC_GC Cuts at: 4729 4729 Size: 7282 NsiI A_TGCA'T Cuts at: 4332 4332 Size: 7282 NspI r_CATG'y Cuts at: 1132 1229 3722 4330 4570 4640 6185 1132 Size: 97 2493 608 240 70 1545 2229 Fragments arranged by size: 2493 2229 1545 608 240 97 70 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7282 PciI A'CATG_T Cuts at: 6181 6181 Size: 7282 PflMI CCAn_nnn'nTGG Cuts at: 1108 2320 4386 1108 Size: 1212 2066 4004 Fragments arranged by size: 4004 2066 1212 PfoI T'CCnGG_A Cuts at: 3741 3741 Size: 7282 PmeI GTTT'AAAC Cuts at: 4586 4586 Size: 7282 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3617 3649 5351 5383 6864 6896 3617 Size: 32 1702 32 1481 32 4003 Fragments arranged by size: 4003 1702 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 4309 4339 4309 Size: 30 7252 PshAI GACnn'nnGTC Cuts at: 1613 3775 1613 Size: 2162 5120 PsiI TTA'TAA Cuts at: 4852 4852 Size: 7282 PspOMI G'GGCC_C Cuts at: 2129 2460 2719 4535 2129 Size: 331 259 1816 4876 Fragments arranged by size: 4876 1816 331 259 PstI C_TGCA'G Cuts at: 830 2232 2271 2482 2836 2997 3039 3381 4071 Size: 1402 39 211 354 161 42 342 690 Cuts at: 4071 4634 830 Size: 563 3478 Fragments arranged by size: 3478 1402 690 563 354 342 211 161 42 39 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7282 PvuII CAG'CTG Cuts at: 2992 3362 2992 Size: 370 6912 SacI G_AGCT'C Cuts at: 2628 3939 4320 2628 Size: 1311 381 5590 Fragments arranged by size: 5590 1311 381 SacII CC_GC'GG Cuts at: 2277 2277 Size: 7282 SalI G'TCGA_C Cuts at: 1936 4624 1936 Size: 2688 4594 SbfI CC_TGCA'GG Cuts at: 2836 4634 2836 Size: 1798 5484 ScaI AGT'ACT Cuts at: 5530 7242 5530 Size: 1712 5570 SexAI A'CCwGG_T Cuts at: 4341 4341 Size: 7282 SfcI C'TryA_G Cuts at: 826 1045 2228 2267 2478 2832 2993 3035 3377 Size: 219 1183 39 211 354 161 42 342 Cuts at: 3377 4067 4630 5765 6446 6637 826 Size: 690 563 1135 681 191 1471 Fragments arranged by size: 1471 1183 1135 690 681 563 354 342 219 211 191 161 42 39 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7282 SgrAI Cr'CCGG_yG Cuts at: 1700 4290 1700 Size: 2590 4692 SmaI CCC'GGG Cuts at: 1872 4609 1872 Size: 2737 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3797 3926 4237 4493 4571 5386 Size: 197 926 1854 129 311 256 78 815 Cuts at: 5386 6287 6549 820 Size: 901 262 1553 Fragments arranged by size: 1854 1553 926 901 815 311 262 256 197 129 78 SnaBI TAC'GTA Cuts at: 493 5073 493 Size: 4580 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7282 SphI G_CATG'C Cuts at: 1132 4570 4640 1132 Size: 3438 70 3774 Fragments arranged by size: 3774 3438 70 SspI AAT'ATT Cuts at: 5 52 5206 5 Size: 47 5154 2081 Fragments arranged by size: 5154 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7282 StyI C'CwwG_G Cuts at: 513 1065 2004 3993 4134 4721 513 Size: 552 939 1989 141 587 3074 Fragments arranged by size: 3074 1989 939 587 552 141 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3608 4276 5309 5326 5479 5664 6109 Size: 1255 2028 668 1033 17 153 185 445 Cuts at: 6109 325 Size: 1498 Fragments arranged by size: 2028 1498 1255 1033 668 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2145 5528 7240 Size: 274 80 33 51 1443 168 3383 1712 Cuts at: 7240 96 Size: 138 Fragments arranged by size: 3383 1712 1443 274 168 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2991 3023 3804 3836 7242 7274 2991 Size: 32 781 32 3406 32 2999 Fragments arranged by size: 3406 2999 781 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3463 1316 Size: 2147 5135 XbaI T'CTAG_A Cuts at: 4618 4618 Size: 7282 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7282 XmaI C'CCGG_G Cuts at: 1870 4607 1870 Size: 2737 4545 XmnI GAAnn'nnTTC Cuts at: 2368 5411 2368 Size: 3043 4239 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4402 5090 276 Size: 53 83 186 936 2868 688 2468 Fragments arranged by size: 2868 2468 936 688 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BbeI BplI BsiWI BstZ17I EcoNI EcoRV FalI FseI FspAI KasI NarI NruI PacI PmlI PspXI PsrI RsrII SanDI SapI SfiI SfoI SrfI SwaI XcmI