Restriction map for
pFN21AB8490
(Linear) (Six-base) MAPSORT of: KIBB8490.seq Check: 1155 from: 1 to: 2577
KIBB8490.orf
With 182 enzymes: SgfI *
October 7, 2009 14:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 162 820 1037 2577
Size: 162 658 217 1540
Fragments arranged by size:
1540 658 217 162
AatII G_ACGT'C
Cuts at: 0 2399 2577
Size: 2399 178
AccI GT'mk_AC
Cuts at: 0 2399 2577
Size: 2399 178
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 350 1337 2560 2577
Size: 350 987 1223 17
Fragments arranged by size:
1223 987 350 17
AfeI AGC'GCT
Cuts at: 0 1978 2577
Size: 1978 599
AflIII A'CryG_T
Cuts at: 0 518 2577
Size: 518 2059
AleI CACnn'nnGTG
Cuts at: 0 1221 2577
Size: 1221 1356
AlwNI CAG_nnn'CTG
Cuts at: 0 41 83 189 222 315 783 2077 2158
Size: 41 42 106 33 93 468 1294 81
Cuts at: 2158 2577
Size: 419
Fragments arranged by size:
1294 468 419 106 93 81 42 41
33
ApaI G_GGCC'C
Cuts at: 0 128 459 718 2534 2577
Size: 128 331 259 1816 43
Fragments arranged by size:
1816 331 259 128 43
ApoI r'AATT_y
Cuts at: 0 2404 2446 2577
Size: 2404 42 131
Fragments arranged by size:
2404 131 42
AvaI C'yCGr_G
Cuts at: 0 711 1309 2577
Size: 711 598 1268
Fragments arranged by size:
1268 711 598
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 541 574 2577
Size: 541 33 2003
Fragments arranged by size:
2003 541 33
BanI G'GyrC_C
Cuts at: 0 832 1428 2577
Size: 832 596 1149
Fragments arranged by size:
1149 832 596
BanII G_rGCy'C
Cuts at: 0 128 459 623 718 929 1121 1531 1880
Size: 128 331 164 95 211 192 410 349
Cuts at: 1880 1934 2315 2375 2534 2577
Size: 54 381 60 159 43
Fragments arranged by size:
410 381 349 331 211 192 164 159
128 95 60 54 43
BbsI GAAGACnn'nnnn_
Cuts at: 0 515 2213 2399 2577
Size: 515 1698 186 178
Fragments arranged by size:
1698 515 186 178
BbvCI CC'TCA_GC
Cuts at: 0 292 1594 2577
Size: 292 1302 983
Fragments arranged by size:
1302 983 292
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 692 726 2577
Size: 692 34 1851
Fragments arranged by size:
1851 692 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 354 2127 2577
Size: 354 1773 450
Fragments arranged by size:
1773 450 354
BfrBI ATG'CAT
Cuts at: 0 2325 2577
Size: 2325 252
BglII A'GATC_T
Cuts at: 0 2543 2577
Size: 2543 34
BlpI GC'TnA_GC
Cuts at: 0 494 928 983 1115 2577
Size: 494 434 55 132 1462
Fragments arranged by size:
1462 494 434 132 55
Bme1580I G_kGCm'C
Cuts at: 0 128 459 570 718 1322 1394 2165 2457
Size: 128 331 111 148 604 72 771 292
Cuts at: 2457 2534 2577
Size: 77 43
Fragments arranged by size:
771 604 331 292 148 128 111 77
72 43
BmgBI CAC'GTC
Cuts at: 0 521 2577
Size: 521 2056
BmrI ACTGGGnnnn_n'
Cuts at: 0 393 1298 2577
Size: 393 905 1279
Fragments arranged by size:
1279 905 393
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 262 292 1144 1204 1620 2196 2329 2577
Size: 262 30 852 60 416 576 133 248
Fragments arranged by size:
852 576 416 262 248 133 60 30
Bpu10I CC'TnA_GC
Cuts at: 0 292 617 815 1594 2577
Size: 292 325 198 779 983
Fragments arranged by size:
983 779 325 292 198
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1777 1906 2217 2473 2551 2577
Size: 1777 129 311 256 78 26
Fragments arranged by size:
1777 311 256 129 78 26
BsaBI GATnn'nnATC
Cuts at: 0 577 2577
Size: 577 2000
BsaHI Gr'CG_yC
Cuts at: 0 2396 2577
Size: 2396 181
BsaWI w'CCGG_w
Cuts at: 0 2051 2577
Size: 2051 526
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 986 1016 1618 1648 2577
Size: 986 30 602 30 929
Fragments arranged by size:
986 929 602 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 436 994 1888 1915 2577
Size: 436 558 894 27 662
Fragments arranged by size:
894 662 558 436 27
BseYI C'CCAG_C
Cuts at: 0 202 242 302 429 440 528 742 792
Size: 202 40 60 127 11 88 214 50
Cuts at: 792 922 1088 1127 1165 1394 1548 2526 2577
Size: 130 166 39 38 229 154 978 51
Fragments arranged by size:
978 229 214 202 166 154 130 127
88 60 51 50 40 39 38 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 175 190 631 1225 2577
Size: 175 15 441 594 1352
Fragments arranged by size:
1352 594 441 175 15
BsiEI CG_ry'CG
Cuts at: 0 212 2577
Size: 212 2365
BsiHKAI G_wGCw'C
Cuts at: 0 509 623 1109 1911 1934 2315 2577
Size: 509 114 486 802 23 381 262
Fragments arranged by size:
802 509 486 381 262 114 23
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1447 2577
Size: 1447 1130
Bsp1286I G_dGCh'C
Cuts at: 0 128 459 509 570 623 718 929 1109
Size: 128 331 50 61 53 95 211 180
Cuts at: 1109 1121 1322 1394 1531 1880 1911 1934 2165
Size: 12 201 72 137 349 31 23 231
Cuts at: 2165 2315 2375 2457 2534 2577
Size: 150 60 82 77 43
Fragments arranged by size:
349 331 231 211 201 180 150 137
128 95 82 77 72 61 60 53
50 43 31 23 12
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 162 230 820 1037 1247 1328 2577
Size: 162 68 590 217 210 81 1249
Fragments arranged by size:
1249 590 217 210 162 81 68
BsrBI CCG'CTC
Cuts at: 0 214 2414 2577
Size: 214 2200 163
Fragments arranged by size:
2200 214 163
BsrDI GCAATG_nn'
Cuts at: 0 381 965 2577
Size: 381 584 1612
Fragments arranged by size:
1612 584 381
BsrFI r'CCGG_y
Cuts at: 0 158 1645 2285 2577
Size: 158 1487 640 292
Fragments arranged by size:
1487 640 292 158
BsrGI T'GTAC_A
Cuts at: 0 140 2577
Size: 140 2437
BssSI C'ACGA_G
Cuts at: 0 1524 1555 2577
Size: 1524 31 1022
Fragments arranged by size:
1524 1022 31
BstAPI GCAn_nnn'nTGC
Cuts at: 0 83 168 189 258 504 2077 2478 2577
Size: 83 85 21 69 246 1573 401 99
Fragments arranged by size:
1573 401 246 99 85 83 69 21
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2031 2577
Size: 2031 546
BstYI r'GATC_y
Cuts at: 0 2543 2577
Size: 2543 34
Bsu36I CC'TnA_GG
Cuts at: 0 483 1614 2577
Size: 483 1131 963
Fragments arranged by size:
1131 963 483
BtgI C'CryG_G
Cuts at: 0 269 590 1988 2577
Size: 269 321 1398 589
Fragments arranged by size:
1398 589 321 269
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 474 1626 2577
Size: 474 1152 951
Fragments arranged by size:
1152 951 474
BtsI GCAGTG_nn'
Cuts at: 0 42 84 105 900 1229 1270 1405 1493
Size: 42 42 21 795 329 41 135 88
Cuts at: 1493 1649 2577
Size: 156 928
Fragments arranged by size:
928 795 329 156 135 88 42 42
41 21
DraIII CAC_nnn'GTG
Cuts at: 0 2381 2577
Size: 2381 196
EaeI y'GGCC_r
Cuts at: 0 111 209 593 1938 2577
Size: 111 98 384 1345 639
Fragments arranged by size:
1345 639 384 111 98
EagI C'GGCC_G
Cuts at: 0 209 2577
Size: 209 2368
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1762 2577
Size: 1762 815
EcoICRI GAG'CTC
Cuts at: 0 621 1932 2313 2577
Size: 621 1311 381 264
Fragments arranged by size:
1311 621 381 264
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 262 292 350 1144 1204 1337 1620 2196
Size: 262 30 58 794 60 133 283 576
Cuts at: 2196 2329 2560 2577
Size: 133 231 17
Fragments arranged by size:
794 576 283 262 231 133 133 60
58 30 17
EcoO109I rG'GnC_Cy
Cuts at: 0 125 186 390 715 2304 2334 2530 2577
Size: 125 61 204 325 1589 30 196 47
Fragments arranged by size:
1589 325 204 196 125 61 47 30
EcoRI G'AATT_C
Cuts at: 0 2404 2446 2577
Size: 2404 42 131
Fragments arranged by size:
2404 131 42
FspI TGC'GCA
Cuts at: 0 386 2577
Size: 386 2191
HaeII r_GCGC'y
Cuts at: 0 277 302 1980 2513 2577
Size: 277 25 1678 533 64
Fragments arranged by size:
1678 533 277 64 25
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1444 1476 2577
Size: 1444 32 1101
Fragments arranged by size:
1444 1101 32
MslI CAynn'nnrTG
Cuts at: 0 1221 1498 2326 2560 2577
Size: 1221 277 828 234 17
Fragments arranged by size:
1221 828 277 234 17
MspA1I CmG'CkG
Cuts at: 0 62 271 700 987 1357 2301 2577
Size: 62 209 429 287 370 944 276
Fragments arranged by size:
944 429 370 287 276 209 62
NaeI GCC'GGC
Cuts at: 0 1647 2287 2577
Size: 1647 640 290
Fragments arranged by size:
1647 640 290
NcoI C'CATG_G
Cuts at: 0 1988 2577
Size: 1988 589
NdeI CA'TA_TG
Cuts at: 0 2327 2577
Size: 2327 250
NgoMIV G'CCGG_C
Cuts at: 0 1645 2285 2577
Size: 1645 640 292
Fragments arranged by size:
1645 640 292
NsiI A_TGCA'T
Cuts at: 0 2327 2577
Size: 2327 250
NspI r_CATG'y
Cuts at: 0 1717 2325 2565 2577
Size: 1717 608 240 12
Fragments arranged by size:
1717 608 240 12
PflMI CCAn_nnn'nTGG
Cuts at: 0 315 2381 2577
Size: 315 2066 196
Fragments arranged by size:
2066 315 196
PfoI T'CCnGG_A
Cuts at: 0 1736 2577
Size: 1736 841
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1612 1644 2577
Size: 1612 32 933
Fragments arranged by size:
1612 933 32
PpuMI rG'GwC_Cy
Cuts at: 0 2304 2334 2577
Size: 2304 30 243
Fragments arranged by size:
2304 243 30
PshAI GACnn'nnGTC
Cuts at: 0 1770 2577
Size: 1770 807
PspOMI G'GGCC_C
Cuts at: 0 124 455 714 2530 2577
Size: 124 331 259 1816 47
Fragments arranged by size:
1816 331 259 124 47
PstI C_TGCA'G
Cuts at: 0 227 266 477 831 992 1034 1376 2066
Size: 227 39 211 354 161 42 342 690
Cuts at: 2066 2577
Size: 511
Fragments arranged by size:
690 511 354 342 227 211 161 42
39
PvuII CAG'CTG
Cuts at: 0 987 1357 2577
Size: 987 370 1220
Fragments arranged by size:
1220 987 370
SacI G_AGCT'C
Cuts at: 0 623 1934 2315 2577
Size: 623 1311 381 262
Fragments arranged by size:
1311 623 381 262
SacII CC_GC'GG
Cuts at: 0 272 2577
Size: 272 2305
SbfI CC_TGCA'GG
Cuts at: 0 831 2577
Size: 831 1746
SexAI A'CCwGG_T
Cuts at: 0 2336 2577
Size: 2336 241
SfcI C'TryA_G
Cuts at: 0 223 262 473 827 988 1030 1372 2062
Size: 223 39 211 354 161 42 342 690
Cuts at: 2062 2577
Size: 515
Fragments arranged by size:
690 515 354 342 223 211 161 42
39
SgrAI Cr'CCGG_yG
Cuts at: 0 2285 2577
Size: 2285 292
SmlI C'TyrA_G
Cuts at: 0 1792 1921 2232 2488 2566 2577
Size: 1792 129 311 256 78 11
Fragments arranged by size:
1792 311 256 129 78 11
SphI G_CATG'C
Cuts at: 0 2565 2577
Size: 2565 12
StyI C'CwwG_G
Cuts at: 0 1988 2129 2577
Size: 1988 141 448
Fragments arranged by size:
1988 448 141
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1603 2271 2577
Size: 1603 668 306
Fragments arranged by size:
1603 668 306
TatI w'GTAC_w
Cuts at: 0 140 2577
Size: 140 2437
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 986 1018 1799 1831 2577
Size: 986 32 781 32 746
Fragments arranged by size:
986 781 746 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1458 2577
Size: 1458 1119
XmnI GAAnn'nnTTC
Cuts at: 0 363 2577
Size: 363 2214
ZraI GAC'GTC
Cuts at: 0 2397 2577
Size: 2397 180
Enzymes that do cut:
AarI AatII AccI AcuI AfeI AflIII AleI AlwNI
ApaI ApoI AvaI BaeI BanI BanII BbsI BbvCI
BcgI BciVI BfrBI BglII BlpI Bme1580I BmgBI BmrI
BpmI Bpu10I BpuEI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraIII EaeI EagI EciI EcoICRI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I MslI MspA1I
NaeI NcoI NdeI NgoMIV NsiI NspI PflMI PfoI
PpiI PpuMI PshAI PspOMI PstI PvuII SacI SacII
SbfI SexAI SfcI SgrAI SmlI SphI StyI TaqII
TatI TstI Tth111I XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AflII AgeI AhdI AlfI AloI ApaLI
AscI AseI AsiSI AvrII BamHI BbeI BclI BglI
BmtI BplI BsaI BsaAI BsiWI BsmI BspEI BspHI
BssHII BstBI BstEII BstZ17I ClaI CspCI DraI DrdI
EarI EcoNI EcoRV FalI FseI FspAI HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI NarI
NheI NotI NruI PacI PasI PciI PmeI PmlI
PsiI PspXI PsrI PvuI RsrII SalI SanDI SapI
ScaI SfiI SfoI SgfI SmaI SnaBI SpeI SrfI
SspI StuI SwaI XbaI XcmI XhoI XmaI