(Circular) (Six-base) MAPSORT of: pFN21AB7988.seq Check: 8411 from: 1 to: 5680 pFN21AB7988 5680 bp With 182 enzymes: SgfI * April 15, 2010 09:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3490 278 Size: 53 83 186 936 1954 2468 Fragments arranged by size: 2468 1954 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3023 1937 Size: 1086 4594 Acc65I G'GTAC_C Cuts at: 3001 3001 Size: 5680 AclI AA'CG_TT Cuts at: 1563 3807 4180 1563 Size: 2244 373 3063 Fragments arranged by size: 3063 2244 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3743 5127 5395 1632 Size: 2111 1384 268 1917 Fragments arranged by size: 2111 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5483 AflIII A'CryG_T Cuts at: 1203 2505 2907 4579 5556 1203 Size: 1302 402 1672 977 1327 Fragments arranged by size: 1672 1327 1302 977 402 AgeI A'CCGG_T Cuts at: 4511 4511 Size: 5680 AhdI GACnn_n'nnGTC Cuts at: 4409 4409 Size: 5680 AleI CACnn'nnGTG Cuts at: 1166 2198 1166 Size: 1032 4648 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2546 1184 Size: 1362 4318 AlwNI CAG_nnn'CTG Cuts at: 2710 3423 4995 2710 Size: 713 1572 3395 Fragments arranged by size: 3395 1572 713 ApaI G_GGCC'C Cuts at: 2262 2262 Size: 5680 ApaLI G'TGCA_C Cuts at: 3737 4893 3737 Size: 1156 4524 ApoI r'AATT_y Cuts at: 1493 2989 3215 3367 5476 1493 Size: 1496 226 152 2109 1697 Fragments arranged by size: 2109 1697 1496 226 152 AseI AT'TA_AT Cuts at: 160 1032 4234 160 Size: 872 3202 1606 Fragments arranged by size: 3202 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5680 AvaI C'yCGr_G Cuts at: 1870 1943 2117 2180 2255 2442 3005 1870 Size: 73 174 63 75 187 563 4545 Fragments arranged by size: 4545 563 187 174 75 73 63 BamHI G'GATC_C Cuts at: 3010 3377 3010 Size: 367 5313 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2140 2833 2918 3001 4456 Size: 325 217 627 353 693 85 83 1455 Cuts at: 4456 618 Size: 1842 Fragments arranged by size: 1842 1455 693 627 353 325 217 85 83 BanII G_rGCy'C Cuts at: 1391 2111 2207 2262 2999 1391 Size: 720 96 55 737 4072 Fragments arranged by size: 4072 737 720 96 55 BbeI G_GCGC'C Cuts at: 2144 2144 Size: 5680 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2501 928 Size: 971 602 4107 Fragments arranged by size: 4107 971 602 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2908 2942 3871 3905 1903 Size: 34 50 34 887 34 929 34 3678 Fragments arranged by size: 3678 929 887 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3572 4782 3572 Size: 1210 4470 BclI T'GATC_A Cuts at: 1548 1548 Size: 5680 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2222 2289 4291 Size: 107 122 71 631 605 550 67 2002 Cuts at: 4291 136 Size: 1525 Fragments arranged by size: 2002 1525 631 605 550 122 107 71 67 BglII A'GATC_T Cuts at: 5675 5675 Size: 5680 BlpI GC'TnA_GC Cuts at: 3097 3097 Size: 5680 Bme1580I G_kGCm'C Cuts at: 1790 2262 2838 2921 3741 4897 1790 Size: 472 576 83 820 1156 2573 Fragments arranged by size: 2573 1156 820 576 472 83 BmgBI CAC'GTC Cuts at: 1318 2908 1318 Size: 1590 4090 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2150 2710 3411 Size: 416 53 83 399 24 733 560 701 Cuts at: 3411 4369 442 Size: 958 1753 Fragments arranged by size: 1753 958 733 701 560 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5680 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4340 1239 Size: 3101 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3805 4670 4968 3805 Size: 865 298 4517 Fragments arranged by size: 4517 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4343 882 Size: 617 2844 2219 Fragments arranged by size: 2844 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3471 493 Size: 711 2267 2702 Fragments arranged by size: 2702 2267 711 BsaBI GATnn'nnATC Cuts at: 5674 5674 Size: 5680 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2031 2141 3487 3869 Size: 53 83 186 936 498 110 1346 382 Cuts at: 3869 4504 275 Size: 635 1451 Fragments arranged by size: 1451 1346 936 635 498 382 186 110 83 53 BsaWI w'CCGG_w Cuts at: 2325 2867 4113 4511 4785 4932 5343 2325 Size: 542 1246 398 274 147 411 2662 Fragments arranged by size: 2662 1246 542 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5455 Fragments arranged by size: 5455 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2380 2423 1186 Size: 1194 43 4443 Fragments arranged by size: 4443 1194 43 BseYI C'CCAG_C Cuts at: 2095 2279 2628 2680 2855 2877 4883 5379 2095 Size: 184 349 52 175 22 2006 496 2396 Fragments arranged by size: 2396 2006 496 349 184 175 52 22 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2468 2740 1163 Size: 573 732 272 4103 Fragments arranged by size: 4103 732 573 272 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2640 2733 3130 3891 4919 1540 Size: 275 186 639 93 397 761 1028 2301 Fragments arranged by size: 2301 1028 761 639 397 275 186 93 BsiHKAI G_wGCw'C Cuts at: 2606 2958 3741 3826 4897 2606 Size: 352 783 85 1071 3389 Fragments arranged by size: 3389 1071 783 352 85 BsmI GAATG_Cn' Cuts at: 3191 3284 3191 Size: 93 5587 BsmBI CGTCTCn'nnnn_ Cuts at: 2869 2869 Size: 5680 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2111 2207 2262 2606 2838 2921 2958 Size: 399 321 96 55 344 232 83 37 Cuts at: 2958 2999 3741 3826 4897 1391 Size: 41 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 344 321 232 96 85 83 55 41 37 BspEI T'CCGG_A Cuts at: 5343 5343 Size: 5680 BspHI T'CATG_A Cuts at: 3569 5299 3569 Size: 1730 3950 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2440 3035 844 Size: 1596 595 3489 Fragments arranged by size: 3489 1596 595 BsrBI CCG'CTC Cuts at: 1394 1823 3567 1394 Size: 429 1744 3507 Fragments arranged by size: 3507 1744 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4175 4349 65 Size: 1183 2927 174 1396 Fragments arranged by size: 2927 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2288 2674 2732 4324 4511 1700 Size: 110 478 386 58 1592 187 2869 Fragments arranged by size: 2869 1592 478 386 187 110 58 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5680 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5680 BssSI C'ACGA_G Cuts at: 3740 4752 3740 Size: 1012 4668 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5680 BstBI TT'CG_AA Cuts at: 4484 4484 Size: 5680 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5680 BstXI CCAn_nnnn'nTGG Cuts at: 2752 2770 2752 Size: 18 5662 BstYI r'GATC_y Cuts at: 1972 3010 3377 3762 3779 5231 5317 5329 5675 Size: 1038 367 385 17 1452 86 12 346 Cuts at: 5675 1972 Size: 1977 Fragments arranged by size: 1977 1452 1038 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2586 2697 2751 513 Size: 552 1521 111 54 3442 Fragments arranged by size: 3442 1521 552 111 54 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2911 487 Size: 683 56 1685 3256 Fragments arranged by size: 3256 1685 683 56 BtsI GCAGTG_nn' Cuts at: 2417 2525 3196 3990 4010 2417 Size: 108 671 794 20 4087 Fragments arranged by size: 4087 794 671 108 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5680 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5645 DraI TTT'AAA Cuts at: 2984 2984 Size: 5680 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4687 809 Size: 507 3371 1802 Fragments arranged by size: 3371 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2290 2637 2730 Size: 54 1425 50 275 18 460 347 93 Cuts at: 2730 3127 4016 5645 8 Size: 397 889 1629 43 Fragments arranged by size: 1629 1425 889 460 397 347 275 93 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2637 2730 3127 1537 Size: 275 825 93 397 4090 Fragments arranged by size: 4090 825 397 275 93 EarI CTCTTCn'nnn_ Cuts at: 1353 3610 1353 Size: 2257 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2727 2927 4267 4641 4787 387 Size: 1064 138 1138 200 1340 374 146 1280 Fragments arranged by size: 1340 1280 1138 1064 374 200 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3743 4340 5127 5395 1239 Size: 393 2111 597 787 268 1524 Fragments arranged by size: 2111 1524 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2134 2259 2134 Size: 125 5555 EcoRI G'AATT_C Cuts at: 2989 3367 2989 Size: 378 5302 FspI TGC'GCA Cuts at: 4186 4186 Size: 5680 HaeII r_GCGC'y Cuts at: 1678 2144 2235 2323 2681 2951 4827 1678 Size: 466 91 88 358 270 1876 2531 Fragments arranged by size: 2531 1876 466 358 270 91 88 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2436 2468 4321 4353 4395 Size: 32 135 32 864 32 1853 32 42 Cuts at: 4395 4427 1373 Size: 32 2626 Fragments arranged by size: 2626 1853 864 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3024 3270 669 Size: 988 281 1086 246 3079 Fragments arranged by size: 3079 1086 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5680 HpaI GTT'AAC Cuts at: 3270 3270 Size: 5680 KasI G'GCGC_C Cuts at: 2140 2140 Size: 5680 KpnI G_GTAC'C Cuts at: 3005 3005 Size: 5680 MfeI C'AATT_G Cuts at: 3279 3279 Size: 5680 MluI A'CGCG_T Cuts at: 5556 5556 Size: 5680 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2136 2340 2787 2805 4794 4978 1305 Size: 831 204 447 18 1989 184 2007 Fragments arranged by size: 2007 1989 831 447 204 184 18 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3858 Fragments arranged by size: 3858 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2198 2399 3638 3997 4156 518 Size: 648 1032 201 1239 359 159 2042 Fragments arranged by size: 2042 1239 1032 648 359 201 159 MspA1I CmG'CkG Cuts at: 1606 2576 2628 2662 3096 3773 4921 5166 1606 Size: 970 52 34 434 677 1148 245 2120 Fragments arranged by size: 2120 1148 970 677 434 245 52 34 NaeI GCC'GGC Cuts at: 2290 2676 2290 Size: 386 5294 NarI GG'CG_CC Cuts at: 2141 2141 Size: 5680 NcoI C'CATG_G Cuts at: 513 1065 2586 2697 2751 513 Size: 552 1521 111 54 3442 Fragments arranged by size: 3442 1521 552 111 54 NdeI CA'TA_TG Cuts at: 387 387 Size: 5680 NgoMIV G'CCGG_C Cuts at: 2288 2674 2288 Size: 386 5294 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5680 NotI GC'GGCC_GC Cuts at: 2637 3127 2637 Size: 490 5190 NspI r_CATG'y Cuts at: 1132 1229 3038 4583 1132 Size: 97 1809 1545 2229 Fragments arranged by size: 2229 1809 1545 97 PasI CC'CwG_GG Cuts at: 1348 2263 1348 Size: 915 4765 PciI A'CATG_T Cuts at: 4579 4579 Size: 5680 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5680 PfoI T'CCnGG_A Cuts at: 2025 2025 Size: 5680 PmeI GTTT'AAAC Cuts at: 2984 2984 Size: 5680 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3749 3781 5262 5294 3749 Size: 32 1481 32 4135 Fragments arranged by size: 4135 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2134 2134 Size: 5680 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5680 PsiI TTA'TAA Cuts at: 3250 3250 Size: 5680 PspOMI G'GGCC_C Cuts at: 2258 2258 Size: 5680 PstI C_TGCA'G Cuts at: 830 2799 2888 3032 830 Size: 1969 89 144 3478 Fragments arranged by size: 3478 1969 144 89 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5680 PvuII CAG'CTG Cuts at: 2662 2662 Size: 5680 RsrII CG'GwC_CG Cuts at: 2735 2735 Size: 5680 SalI G'TCGA_C Cuts at: 1936 3022 1936 Size: 1086 4594 SbfI CC_TGCA'GG Cuts at: 3032 3032 Size: 5680 ScaI AGT'ACT Cuts at: 3928 5640 3928 Size: 1712 3968 SfcI C'TryA_G Cuts at: 826 1045 2795 2884 3028 4163 4844 5035 826 Size: 219 1750 89 144 1135 681 191 1471 Fragments arranged by size: 1750 1471 1135 681 219 191 144 89 SfoI GGC'GCC Cuts at: 2142 2142 Size: 5680 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5680 SgrAI Cr'CCGG_yG Cuts at: 1700 2674 1700 Size: 974 4706 SmaI CCC'GGG Cuts at: 1872 2119 2182 2257 3007 1872 Size: 247 63 75 750 4545 Fragments arranged by size: 4545 750 247 75 63 SmlI C'TyrA_G Cuts at: 820 1017 1943 3784 4685 4947 820 Size: 197 926 1841 901 262 1553 Fragments arranged by size: 1841 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3471 493 Size: 2978 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5680 SphI G_CATG'C Cuts at: 1132 3038 1132 Size: 1906 3774 SrfI GCCC'GGGC Cuts at: 2257 2257 Size: 5680 SspI AAT'ATT Cuts at: 5 52 3604 5 Size: 47 3552 2081 Fragments arranged by size: 3552 2081 47 StuI AGG'CCT Cuts at: 1517 2544 1517 Size: 1027 4653 StyI C'CwwG_G Cuts at: 513 1065 2586 2697 2751 3119 513 Size: 552 1521 111 54 368 3074 Fragments arranged by size: 3074 1521 552 368 111 54 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2450 3707 3724 3877 4062 4507 325 Size: 1255 870 1257 17 153 185 445 1498 Fragments arranged by size: 1498 1257 1255 870 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3926 5638 96 Size: 274 80 33 51 1443 1949 1712 138 Fragments arranged by size: 1949 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5640 5672 5640 Size: 32 5648 Tth111I GACn'n_nGTC Cuts at: 1316 2437 1316 Size: 1121 4559 XbaI T'CTAG_A Cuts at: 3016 3016 Size: 5680 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5680 XmaI C'CCGG_G Cuts at: 1870 2117 2180 2255 3005 1870 Size: 247 63 75 750 4545 Fragments arranged by size: 4545 750 247 75 63 XmnI GAAnn'nnTTC Cuts at: 3809 3809 Size: 5680 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3488 276 Size: 53 83 186 936 1954 2468 Fragments arranged by size: 2468 1954 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PspXI PsrI SacI SacII SanDI SapI SexAI SfiI SwaI XcmI