(Circular) (Six-base) MAPSORT of: pFN21AB7943.seq Check: 4742 from: 1 to: 5836 pFN21AB7943 5836 bp With 182 enzymes: SgfI * April 15, 2010 09:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2326 2326 Size: 5836 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3646 278 Size: 53 83 186 936 2110 2468 Fragments arranged by size: 2468 2110 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3179 1937 Size: 1242 4594 Acc65I G'GTAC_C Cuts at: 3157 3157 Size: 5836 AclI AA'CG_TT Cuts at: 1563 3135 3963 4336 1563 Size: 1572 828 373 3063 Fragments arranged by size: 3063 1572 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2106 3899 5283 5551 1632 Size: 474 1793 1384 268 1917 Fragments arranged by size: 1917 1793 1384 474 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5639 AflIII A'CryG_T Cuts at: 1203 4735 5712 1203 Size: 3532 977 1327 Fragments arranged by size: 3532 1327 977 AgeI A'CCGG_T Cuts at: 4667 4667 Size: 5836 AhdI GACnn_n'nnGTC Cuts at: 4565 4565 Size: 5836 AleI CACnn'nnGTG Cuts at: 1166 2714 2985 1166 Size: 1548 271 4017 Fragments arranged by size: 4017 1548 271 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2333 1184 Size: 1149 4687 AlwNI CAG_nnn'CTG Cuts at: 2609 2959 2972 3579 5151 2609 Size: 350 13 607 1572 3294 Fragments arranged by size: 3294 1572 607 350 13 ApaI G_GGCC'C Cuts at: 2744 2744 Size: 5836 ApaLI G'TGCA_C Cuts at: 2308 2831 3893 5049 2308 Size: 523 1062 1156 3095 Fragments arranged by size: 3095 1156 1062 523 ApoI r'AATT_y Cuts at: 1493 3145 3371 3523 5632 1493 Size: 1652 226 152 2109 1697 Fragments arranged by size: 2109 1697 1652 226 152 AseI AT'TA_AT Cuts at: 160 1032 4390 160 Size: 872 3358 1606 Fragments arranged by size: 3358 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5836 AvaI C'yCGr_G Cuts at: 1870 1943 2237 2328 2567 3161 1870 Size: 73 294 91 239 594 4545 Fragments arranged by size: 4545 594 294 239 91 73 BamHI G'GATC_C Cuts at: 3166 3533 3166 Size: 367 5469 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3157 4612 618 Size: 325 217 627 1370 1455 1842 Fragments arranged by size: 1842 1455 1370 627 325 217 BanII G_rGCy'C Cuts at: 1391 2429 2636 2744 2930 3155 1391 Size: 1038 207 108 186 225 4072 Fragments arranged by size: 4072 1038 225 207 186 108 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2270 928 Size: 971 371 4494 Fragments arranged by size: 4494 971 371 BbvCI CC'TCA_GC Cuts at: 2421 2610 2421 Size: 189 5647 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2498 2532 4027 4061 1903 Size: 34 50 34 477 34 1495 34 3678 Fragments arranged by size: 3678 1495 477 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3728 4938 3728 Size: 1210 4626 BclI T'GATC_A Cuts at: 1548 1548 Size: 5836 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2093 2739 4447 Size: 107 122 71 631 605 421 646 1708 Cuts at: 4447 136 Size: 1525 Fragments arranged by size: 1708 1525 646 631 605 421 122 107 71 BglII A'GATC_T Cuts at: 5831 5831 Size: 5836 BlpI GC'TnA_GC Cuts at: 2145 2229 2520 2771 3253 2145 Size: 84 291 251 482 4728 Fragments arranged by size: 4728 482 291 251 84 Bme1580I G_kGCm'C Cuts at: 1790 2312 2744 2835 2898 3897 5053 1790 Size: 522 432 91 63 999 1156 2573 Fragments arranged by size: 2573 1156 999 522 432 91 63 BmgBI CAC'GTC Cuts at: 1318 2495 1318 Size: 1177 4659 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3567 4525 442 Size: 416 53 83 399 24 2150 958 1753 Fragments arranged by size: 2150 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5836 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2387 2445 4496 1239 Size: 1148 58 2051 2579 Fragments arranged by size: 2579 2051 1148 58 Bpu10I CC'TnA_GC Cuts at: 2421 2610 2421 Size: 189 5647 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3961 4826 5124 3961 Size: 865 298 4673 Fragments arranged by size: 4673 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3003 4499 882 Size: 617 1504 1496 2219 Fragments arranged by size: 2219 1504 1496 617 BsaAI yAC'GTr Cuts at: 493 1204 3627 493 Size: 711 2423 2702 Fragments arranged by size: 2702 2423 711 BsaBI GATnn'nnATC Cuts at: 5830 5830 Size: 5836 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2907 3643 4025 4660 Size: 53 83 186 936 1374 736 382 635 Cuts at: 4660 275 Size: 1451 Fragments arranged by size: 1451 1374 936 736 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2367 4269 4667 4941 5088 5499 2367 Size: 1902 398 274 147 411 2704 Fragments arranged by size: 2704 1902 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2695 2725 3092 3122 1178 Size: 30 165 30 1292 30 367 30 3892 Fragments arranged by size: 3892 1292 367 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2603 2991 1186 Size: 1417 388 4031 Fragments arranged by size: 4031 1417 388 BseYI C'CCAG_C Cuts at: 2088 2097 2187 2469 2656 2923 3019 5039 5535 Size: 9 90 282 187 267 96 2020 496 Cuts at: 5535 2088 Size: 2389 Fragments arranged by size: 2389 2020 496 282 267 187 96 90 9 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2021 2057 2354 2918 2997 1163 Size: 573 285 36 297 564 79 4002 Fragments arranged by size: 4002 573 564 297 285 79 36 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3286 4047 5075 1540 Size: 275 186 1285 761 1028 2301 Fragments arranged by size: 2301 1285 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2312 2835 3897 3982 5053 2312 Size: 523 1062 85 1071 3095 Fragments arranged by size: 3095 1071 1062 523 85 BsmI GAATG_Cn' Cuts at: 3347 3440 3347 Size: 93 5743 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2312 2429 2636 2744 2835 2898 2930 Size: 399 522 117 207 108 91 63 32 Cuts at: 2930 3155 3897 3982 5053 1391 Size: 225 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 522 399 225 207 117 108 91 85 63 32 BspEI T'CCGG_A Cuts at: 2367 5499 2367 Size: 3132 2704 BspHI T'CATG_A Cuts at: 3725 5455 3725 Size: 1730 4106 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2079 2325 2326 3087 3191 844 Size: 1235 246 1 761 104 3489 Fragments arranged by size: 3489 1235 761 246 104 1 BsrBI CCG'CTC Cuts at: 1394 1823 3723 1394 Size: 429 1900 3507 Fragments arranged by size: 3507 1900 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4331 4505 65 Size: 1183 3083 174 1396 Fragments arranged by size: 3083 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2115 4480 4667 1700 Size: 110 305 2365 187 2869 Fragments arranged by size: 2869 2365 305 187 110 BsrGI T'GTAC_A Cuts at: 96 2064 96 Size: 1968 3868 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5836 BssSI C'ACGA_G Cuts at: 3896 4908 3896 Size: 1012 4824 BstAPI GCAn_nnn'nTGC Cuts at: 794 2972 794 Size: 2178 3658 BstBI TT'CG_AA Cuts at: 2847 4640 2847 Size: 1793 4043 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5836 BstXI CCAn_nnnn'nTGG Cuts at: 2827 2827 Size: 5836 BstYI r'GATC_y Cuts at: 1972 3166 3533 3918 3935 5387 5473 5485 5831 Size: 1194 367 385 17 1452 86 12 346 Cuts at: 5831 1972 Size: 1977 Fragments arranged by size: 1977 1452 1194 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2322 2322 Size: 5836 BtgI C'CryG_G Cuts at: 513 1065 2431 513 Size: 552 1366 3918 Fragments arranged by size: 3918 1366 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5097 Fragments arranged by size: 5097 683 56 BtsI GCAGTG_nn' Cuts at: 2033 2930 2935 3352 4146 4166 2033 Size: 897 5 417 794 20 3703 Fragments arranged by size: 3703 897 794 417 20 5 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5836 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5801 DraI TTT'AAA Cuts at: 3140 3140 Size: 5836 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4843 809 Size: 507 3527 1802 Fragments arranged by size: 3527 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2301 2814 3283 Size: 54 1425 50 275 18 471 513 469 Cuts at: 3283 4172 5801 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 513 471 469 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3283 1537 Size: 275 1471 4090 Fragments arranged by size: 4090 1471 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2624 2783 3766 1353 Size: 1271 159 983 3423 Fragments arranged by size: 3423 1271 983 159 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2789 4423 4797 4943 387 Size: 1064 138 1200 1634 374 146 1280 Fragments arranged by size: 1634 1280 1200 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2106 2387 2445 3899 4496 5283 5551 Size: 393 474 281 58 1454 597 787 268 Cuts at: 5551 1239 Size: 1524 Fragments arranged by size: 1524 1454 787 597 474 393 281 268 58 EcoNI CCTnn'n_nnAGG Cuts at: 2321 2390 2321 Size: 69 5767 EcoO109I rG'GnC_Cy Cuts at: 2242 2452 2593 2614 2741 2242 Size: 210 141 21 127 5337 Fragments arranged by size: 5337 210 141 127 21 EcoRI G'AATT_C Cuts at: 3145 3523 3145 Size: 378 5458 FspI TGC'GCA Cuts at: 4342 4342 Size: 5836 HaeII r_GCGC'y Cuts at: 1678 2061 2152 4983 1678 Size: 383 91 2831 2531 Fragments arranged by size: 2831 2531 383 91 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2695 2727 4477 4509 4551 Size: 32 135 32 1123 32 1750 32 42 Cuts at: 4551 4583 1373 Size: 32 2626 Fragments arranged by size: 2626 1750 1123 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2911 3180 3426 669 Size: 988 281 973 269 246 3079 Fragments arranged by size: 3079 988 973 281 269 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5836 HpaI GTT'AAC Cuts at: 3426 3426 Size: 5836 KpnI G_GTAC'C Cuts at: 3161 3161 Size: 5836 MfeI C'AATT_G Cuts at: 3435 3435 Size: 5836 MluI A'CGCG_T Cuts at: 5712 5712 Size: 5836 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4950 5134 1305 Size: 3645 184 2007 Fragments arranged by size: 3645 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2303 10 Size: 54 1425 343 471 3543 Fragments arranged by size: 3543 1425 471 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2714 2985 3794 4153 4312 518 Size: 648 1548 271 809 359 159 2042 Fragments arranged by size: 2042 1548 809 648 359 271 159 MspA1I CmG'CkG Cuts at: 1606 2473 2770 2879 2923 2972 3252 3929 5077 Size: 867 297 109 44 49 280 677 1148 Cuts at: 5077 5322 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 867 677 297 280 245 109 49 44 NcoI C'CATG_G Cuts at: 513 1065 2431 513 Size: 552 1366 3918 Fragments arranged by size: 3918 1366 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5836 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5836 NotI GC'GGCC_GC Cuts at: 3283 3283 Size: 5836 NspI r_CATG'y Cuts at: 1132 1229 2105 2132 3194 4739 1132 Size: 97 876 27 1062 1545 2229 Fragments arranged by size: 2229 1545 1062 876 97 27 PasI CC'CwG_GG Cuts at: 1348 2598 2636 2723 3112 1348 Size: 1250 38 87 389 4072 Fragments arranged by size: 4072 1250 389 87 38 PciI A'CATG_T Cuts at: 4735 4735 Size: 5836 PflMI CCAn_nnn'nTGG Cuts at: 1108 2729 2809 1108 Size: 1621 80 4135 Fragments arranged by size: 4135 1621 80 PmeI GTTT'AAAC Cuts at: 3140 3140 Size: 5836 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2015 2047 3905 3937 5418 5450 2015 Size: 32 1858 32 1481 32 2401 Fragments arranged by size: 2401 1858 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2593 2614 2593 Size: 21 5815 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5836 PsiI TTA'TAA Cuts at: 3406 3406 Size: 5836 PspOMI G'GGCC_C Cuts at: 2740 2740 Size: 5836 PspXI vC'TCGA_Gb Cuts at: 2237 2237 Size: 5836 PstI C_TGCA'G Cuts at: 830 2391 2790 2878 3188 830 Size: 1561 399 88 310 3478 Fragments arranged by size: 3478 1561 399 310 88 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5836 PvuII CAG'CTG Cuts at: 2473 2473 Size: 5836 RsrII CG'GwC_CG Cuts at: 2581 2581 Size: 5836 SalI G'TCGA_C Cuts at: 1936 3178 1936 Size: 1242 4594 SapI GCTCTTCn'nnn_ Cuts at: 2624 2624 Size: 5836 SbfI CC_TGCA'GG Cuts at: 3188 3188 Size: 5836 ScaI AGT'ACT Cuts at: 4084 5796 4084 Size: 1712 4124 SexAI A'CCwGG_T Cuts at: 2826 2826 Size: 5836 SfcI C'TryA_G Cuts at: 826 1045 2387 2786 2874 3184 4319 5000 5191 Size: 219 1342 399 88 310 1135 681 191 Cuts at: 5191 826 Size: 1471 Fragments arranged by size: 1471 1342 1135 681 399 310 219 191 88 SfiI GGCCn_nnn'nGGCC Cuts at: 2739 2739 Size: 5836 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5836 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5836 SmaI CCC'GGG Cuts at: 1872 2330 3163 1872 Size: 458 833 4545 Fragments arranged by size: 4545 833 458 SmlI C'TyrA_G Cuts at: 820 1017 1943 2237 3940 4841 5103 820 Size: 197 926 294 1703 901 262 1553 Fragments arranged by size: 1703 1553 926 901 294 262 197 SnaBI TAC'GTA Cuts at: 493 3627 493 Size: 3134 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5836 SphI G_CATG'C Cuts at: 1132 2105 2132 3194 1132 Size: 973 27 1062 3774 Fragments arranged by size: 3774 1062 973 27 SspI AAT'ATT Cuts at: 5 52 3760 5 Size: 47 3708 2081 Fragments arranged by size: 3708 2081 47 StuI AGG'CCT Cuts at: 1517 3027 1517 Size: 1510 4326 StyI C'CwwG_G Cuts at: 513 1065 2431 2679 3275 513 Size: 552 1366 248 596 3074 Fragments arranged by size: 3074 1366 596 552 248 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3863 3880 4033 4218 4663 325 Size: 1255 2283 17 153 185 445 1498 Fragments arranged by size: 2283 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2064 4082 5794 Size: 274 80 33 51 1443 87 2018 1712 Cuts at: 5794 96 Size: 138 Fragments arranged by size: 2018 1712 1443 274 138 87 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2741 2773 2825 2857 2927 2959 3092 3124 5796 Size: 32 52 32 70 32 133 32 2672 Cuts at: 5796 5828 2741 Size: 32 2749 Fragments arranged by size: 2749 2672 133 70 52 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5836 XbaI T'CTAG_A Cuts at: 3172 3172 Size: 5836 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2713 2824 2713 Size: 111 5725 XhoI C'TCGA_G Cuts at: 1943 2237 1943 Size: 294 5542 XmaI C'CCGG_G Cuts at: 1870 2328 3161 1870 Size: 458 833 4545 Fragments arranged by size: 4545 833 458 XmnI GAAnn'nnTTC Cuts at: 3965 3965 Size: 5836 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3644 276 Size: 53 83 186 936 2110 2468 Fragments arranged by size: 2468 2110 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII RsrII SalI SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PsrI SacI SacII SanDI SfoI SrfI SwaI