Restriction map for
pFN21AB8474
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# NO CUT ENZYMES ( 117 enzymes) #
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AatII Acc65I AccII AclI AcsI
AcyI AflII AflIII AgeI Alw44I
AocI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp718I AspI AviII
AvrII BalI BanI BbeI BbrPI
BcgI BfrI BlnI BsaAI BsaBI
BsaHI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspHI BspLU11I BspMI BsrFI
BsrGI BssHII Bst1107I BstBI BstEII
BstPI BstUI BstZ17I Bsu36I Cfr10I
CpoI DraI DraIII DrdI EagI
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI EcoRV EcoT22I FseI FspI
Hin1I HindIII HpaI KasI KpnI
KspI MaeIII MamI MfeI MluI
MluNI MscI MunI MvnI NaeI
NarI NdeI NgoMIV NotI NruI
NsiI NspV PacI PflFI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RcaI RsrII SacII ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI SspBI
SspI SwaI Tsp45I Tth111I XbaI
XmaCI XmaI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AccIII 59 TCCGGA T^CCGGA
AhdI 125 GACAGAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 125 GACAGAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 1714 CTCGAG C^[CT]CG[GA]G
BbvCI 411 GCTGAGG CCTCAGC(-5/-2)
BciVI 949 GTATCC GTATCC(6/5)
BclI 807 TGATCA T^GATCA
BglII 2021 AGATCT A^GATCT
BsaWI 59 TCCGGA [AT]^CCGG[AT]
BseAI 59 TCCGGA T^CCGGA
BsoBI 1714 CTCGAG C^[CT]CG[GA]G
BspDI 2275 ATCGAT AT^CGAT
BspEI 59 TCCGGA T^CCGGA
BsrDI 1309 GCAATG GCAATG(2/0)
BssSI 394 CTCGTG CACGAG(-5/-1)
ClaI 2275 ATCGAT AT^CGAT
Eam1105I 125 GACAGAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1764 CCTGAAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 807 TGATCA T^GATCA
MroI 59 TCCGGA T^CCGGA
MseI 758 TTAA T^TAA
MslI 282 CACACTAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NheI 1305 GCTAGC G^CTAGC
PaeR7I 1714 CTCGAG C^TCGAG
PflMI 1562 CCAAGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 2253 GACCTACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SalI 1196 GTCGAC G^TCGAC
SbfI 459 CCTGCAGG CCTGCA^GG
Sse8387I 459 CCTGCAGG CCTGCA^GG
StuI 1929 AGGCCT AGG^CCT
TfiI 1600 GAATC G^A[AT]TC
Tru9I 758 TTAA T^TAA
Van91I 1562 CCAAGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 863 CCAATCAGAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1714 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AccI 1196 GTCGAC GT^[AC][GT]AC
1364 GTCTAC
Asp700I 1207 GAACAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1525 GAAGAACTTC
BamHI 519 GGATCC G^GATCC
1802 GGATCC
BsaI 2251 GAGACC GGTCTC(1/5)
2333 GAGACC
BsgI 244 CTGCAC GTGCAG(16/14)
962 GTGCAG
EaeI 290 TGGCCG [CT]^GGCC[GA]
2095 CGGCCA
HgaI 1443 GCGTC GACGC(5/10)
2324 GCGTC
HincII 1196 GTCGAC GT[CT]^[GA]AC
1242 GTTGAC
HindII 1196 GTCGAC GT[CT]^[GA]AC
1242 GTTGAC
MaeII 449 ACGT A^CGT
2258 ACGT
NcoI 1550 CCATGG C^CATGG
2213 CCATGG
NspI 915 ACATGC [GA]CATG^[CT]
2390 ACATGC
SapI 1345 GAAGAGC GCTCTTC(1/4)
2235 GAAGAGC
XmnI 1207 GAACAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1525 GAAGAACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
ApaI AspHI AvaII BanII BbsI
BbvI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BpuAI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrBI BsrI BssKI
BstAPI BstF5I BstNI BstXI BstYI
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraII DsaI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MboI MboII MflI MnlI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TseI Tsp509I TspRI
TthHB8I XhoII