Restriction map for
pFN21AB8474
(Linear) (Six-base) MAPSORT of: KIBB8474.seq Check: 6367 from: 1 to: 2523
KIBB8474.orf
With 182 enzymes: SgfI *
October 7, 2009 14:25 ..
AccI GT'mk_AC
Cuts at: 0 1197 1365 2523
Size: 1197 168 1158
Fragments arranged by size:
1197 1158 168
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 370 1693 1768 1786 1992 2523
Size: 370 1323 75 18 206 531
Fragments arranged by size:
1323 531 370 206 75 18
AhdI GACnn_n'nnGTC
Cuts at: 0 130 2523
Size: 130 2393
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1679 2523
Size: 1679 844
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 136 168 2523
Size: 136 32 2355
Fragments arranged by size:
2355 136 32
AlwNI CAG_nnn'CTG
Cuts at: 0 51 348 840 2142 2523
Size: 51 297 492 1302 381
Fragments arranged by size:
1302 492 381 297 51
ApaI G_GGCC'C
Cuts at: 0 392 535 936 2523
Size: 392 143 401 1587
Fragments arranged by size:
1587 401 392 143
AvaI C'yCGr_G
Cuts at: 0 1714 2523
Size: 1714 809
BamHI G'GATC_C
Cuts at: 0 519 1802 2523
Size: 519 1283 721
Fragments arranged by size:
1283 721 519
BanII G_rGCy'C
Cuts at: 0 392 535 646 936 1418 1460 2044 2132
Size: 392 143 111 290 482 42 584 88
Cuts at: 2132 2523
Size: 391
Fragments arranged by size:
584 482 392 391 290 143 111 88
42
BbsI GAAGACnn'nnnn_
Cuts at: 0 112 1439 1756 2523
Size: 112 1327 317 767
Fragments arranged by size:
1327 767 317 112
BbvCI CC'TCA_GC
Cuts at: 0 412 2523
Size: 412 2111
BciVI GTATCCnnnnn_n'
Cuts at: 0 960 2523
Size: 960 1563
BclI T'GATC_A
Cuts at: 0 807 2523
Size: 807 1716
BglI GCCn_nnn'nGGC
Cuts at: 0 823 1928 2094 2523
Size: 823 1105 166 429
Fragments arranged by size:
1105 823 429 166
BglII A'GATC_T
Cuts at: 0 2021 2523
Size: 2021 502
BlpI GC'TnA_GC
Cuts at: 0 44 686 2126 2467 2523
Size: 44 642 1440 341 56
Fragments arranged by size:
1440 642 341 56 44
Bme1580I G_kGCm'C
Cuts at: 0 392 535 936 1401 2523
Size: 392 143 401 465 1122
Fragments arranged by size:
1122 465 401 392 143
BmrI ACTGGGnnnn_n'
Cuts at: 0 813 1100 2523
Size: 813 287 1423
Fragments arranged by size:
1423 813 287
BmtI G_CTAG'C
Cuts at: 0 1309 2523
Size: 1309 1214
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1977 2523
Size: 1977 546
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 349 1054 1156 1495 1522 1828 2194 2416
Size: 349 705 102 339 27 306 366 222
Cuts at: 2416 2523
Size: 107
Fragments arranged by size:
705 366 349 339 306 222 107 102
27
Bpu10I CC'TnA_GC
Cuts at: 0 194 412 1538 1926 2523
Size: 194 218 1126 388 597
Fragments arranged by size:
1126 597 388 218 194
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 874 1032 1624 1816 1825 1954 2063 2523
Size: 874 158 592 192 9 129 109 460
Fragments arranged by size:
874 592 460 192 158 129 109 9
BsaI GGTCTCn'nnnn_
Cuts at: 0 2245 2327 2523
Size: 2245 82 196
Fragments arranged by size:
2245 196 82
BsaWI w'CCGG_w
Cuts at: 0 59 2523
Size: 59 2464
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 359 389 739 769 1147 1177 2523
Size: 359 30 350 30 378 30 1346
Fragments arranged by size:
1346 378 359 350 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 34 37 271 616 1465 1468 1492 1699
Size: 34 3 234 345 849 3 24 207
Cuts at: 1699 1702 1744 1951 1992 2191 2413 2523
Size: 3 42 207 41 199 222 110
Fragments arranged by size:
849 345 234 222 207 207 199 110
42 41 34 24 3 3 3
BseYI C'CCAG_C
Cuts at: 0 385 1166 1334 1350 1386 2084 2316 2523
Size: 385 781 168 16 36 698 232 207
Fragments arranged by size:
781 698 385 232 207 168 36 16
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 229 983 2523
Size: 229 754 1540
Fragments arranged by size:
1540 754 229
BsiHKAI G_wGCw'C
Cuts at: 0 646 1418 1460 2044 2132 2523
Size: 646 772 42 584 88 391
Fragments arranged by size:
772 646 584 391 88 42
Bsp1286I G_dGCh'C
Cuts at: 0 392 535 646 936 1401 1418 1460 2044
Size: 392 143 111 290 465 17 42 584
Cuts at: 2044 2132 2523
Size: 88 391
Fragments arranged by size:
584 465 392 391 290 143 111 88
42 17
BspEI T'CCGG_A
Cuts at: 0 59 2523
Size: 59 2464
BsrBI CCG'CTC
Cuts at: 0 1734 1863 1925 2523
Size: 1734 129 62 598
Fragments arranged by size:
1734 598 129 62
BsrDI GCAATG_nn'
Cuts at: 0 1316 2523
Size: 1316 1207
BssSI C'ACGA_G
Cuts at: 0 394 2523
Size: 394 2129
BstAPI GCAn_nnn'nTGC
Cuts at: 0 308 376 2391 2477 2523
Size: 308 68 2015 86 46
Fragments arranged by size:
2015 308 86 68 46
BstXI CCAn_nnnn'nTGG
Cuts at: 0 549 564 1095 2523
Size: 549 15 531 1428
Fragments arranged by size:
1428 549 531 15
BstYI r'GATC_y
Cuts at: 0 519 1040 1469 1802 2021 2523
Size: 519 521 429 333 219 502
Fragments arranged by size:
521 519 502 429 333 219
BtgI C'CryG_G
Cuts at: 0 1461 1550 2213 2523
Size: 1461 89 663 310
Fragments arranged by size:
1461 663 310 89
BtsI GCAGTG_nn'
Cuts at: 0 1651 1827 2211 2523
Size: 1651 176 384 312
Fragments arranged by size:
1651 384 312 176
ClaI AT'CG_AT
Cuts at: 0 2276 2523
Size: 2276 247
EaeI y'GGCC_r
Cuts at: 0 290 2095 2523
Size: 290 1805 428
Fragments arranged by size:
1805 428 290
EarI CTCTTCn'nnn_
Cuts at: 0 601 1264 1340 2230 2523
Size: 601 663 76 890 293
Fragments arranged by size:
890 663 601 293 76
EcoICRI GAG'CTC
Cuts at: 0 644 1416 1458 2042 2130 2523
Size: 644 772 42 584 88 393
Fragments arranged by size:
772 644 584 393 88 42
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 349 370 1054 1156 1495 1522 1693 1768
Size: 349 21 684 102 339 27 171 75
Cuts at: 1768 1786 1828 1992 2194 2416 2523
Size: 18 42 164 202 222 107
Fragments arranged by size:
684 349 339 222 202 171 164 107
102 75 42 27 21 18
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1768 2523
Size: 1768 755
EcoO109I rG'GnC_Cy
Cuts at: 0 389 531 933 1134 1836 2060 2223 2485
Size: 389 142 402 201 702 224 163 262
Cuts at: 2485 2523
Size: 38
Fragments arranged by size:
702 402 389 262 224 201 163 142
38
HaeII r_GCGC'y
Cuts at: 0 909 1584 2243 2523
Size: 909 675 659 280
Fragments arranged by size:
909 675 659 280
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 27 59 116 148 934 966 1147 1179
Size: 27 32 57 32 786 32 181 32
Cuts at: 1179 2523
Size: 1344
Fragments arranged by size:
1344 786 181 57 32 32 32 32
27
HincII GTy'rAC
Cuts at: 0 1198 1244 2523
Size: 1198 46 1279
Fragments arranged by size:
1279 1198 46
MslI CAynn'nnrTG
Cuts at: 0 286 2523
Size: 286 2237
MspA1I CmG'CkG
Cuts at: 0 33 327 499 627 894 1102 1851 2139
Size: 33 294 172 128 267 208 749 288
Cuts at: 2139 2312 2523
Size: 173 211
Fragments arranged by size:
749 294 288 267 211 208 173 172
128 33
NcoI C'CATG_G
Cuts at: 0 1550 2213 2523
Size: 1550 663 310
Fragments arranged by size:
1550 663 310
NheI G'CTAG_C
Cuts at: 0 1305 2523
Size: 1305 1218
NspI r_CATG'y
Cuts at: 0 919 2394 2523
Size: 919 1475 129
Fragments arranged by size:
1475 919 129
PasI CC'CwG_GG
Cuts at: 0 526 2523
Size: 526 1997
PflMI CCAn_nnn'nTGG
Cuts at: 0 1568 2523
Size: 1568 955
PfoI T'CCnGG_A
Cuts at: 0 50 1076 1172 1472 1805 2523
Size: 50 1026 96 300 333 718
Fragments arranged by size:
1026 718 333 300 96 50
PpuMI rG'GwC_Cy
Cuts at: 0 1134 1836 2060 2223 2523
Size: 1134 702 224 163 300
Fragments arranged by size:
1134 702 300 224 163
PshAI GACnn'nnGTC
Cuts at: 0 2257 2523
Size: 2257 266
PspOMI G'GGCC_C
Cuts at: 0 388 531 932 2523
Size: 388 143 401 1591
Fragments arranged by size:
1591 401 388 143
PspXI vC'TCGA_Gb
Cuts at: 0 1714 2523
Size: 1714 809
PstI C_TGCA'G
Cuts at: 0 236 313 366 464 674 737 845 1610
Size: 236 77 53 98 210 63 108 765
Cuts at: 1610 1886 1898 2387 2523
Size: 276 12 489 136
Fragments arranged by size:
765 489 276 236 210 136 108 98
77 63 53 12
PvuII CAG'CTG
Cuts at: 0 327 499 627 1102 1851 2523
Size: 327 172 128 475 749 672
Fragments arranged by size:
749 672 475 327 172 128
SacI G_AGCT'C
Cuts at: 0 646 1418 1460 2044 2132 2523
Size: 646 772 42 584 88 391
Fragments arranged by size:
772 646 584 391 88 42
SalI G'TCGA_C
Cuts at: 0 1196 2523
Size: 1196 1327
SapI GCTCTTCn'nnn_
Cuts at: 0 1340 2230 2523
Size: 1340 890 293
Fragments arranged by size:
1340 890 293
SbfI CC_TGCA'GG
Cuts at: 0 464 2523
Size: 464 2059
SfcI C'TryA_G
Cuts at: 0 232 309 362 418 460 670 733 841
Size: 232 77 53 56 42 210 63 108
Cuts at: 841 1606 1693 1882 1894 2383 2523
Size: 765 87 189 12 489 140
Fragments arranged by size:
765 489 232 210 189 140 108 87
77 63 56 53 42 12
SmlI C'TyrA_G
Cuts at: 0 853 1011 1639 1714 1831 1840 1933 2078
Size: 853 158 628 75 117 9 93 145
Cuts at: 2078 2523
Size: 445
Fragments arranged by size:
853 628 445 158 145 117 93 75
9
StuI AGG'CCT
Cuts at: 0 1931 2523
Size: 1931 592
StyI C'CwwG_G
Cuts at: 0 1550 1562 2213 2523
Size: 1550 12 651 310
Fragments arranged by size:
1550 651 310 12
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 870 2523
Size: 870 1653
XhoI C'TCGA_G
Cuts at: 0 1714 2523
Size: 1714 809
XmnI GAAnn'nnTTC
Cuts at: 0 1211 1529 2523
Size: 1211 318 994
Fragments arranged by size:
1211 994 318
Enzymes that do cut:
AccI AcuI AhdI AlfI AloI AlwNI ApaI AvaI
BamHI BanII BbsI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I
BspEI BsrBI BsrDI BssSI BstAPI BstXI BstYI BtgI
BtsI ClaI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I
HaeII Hin4I HincII MslI MspA1I NcoI NheI NspI
PasI PflMI PfoI PpuMI PshAI PspOMI PspXI PstI
PvuII SacI SalI SapI SbfI SfcI SmlI StuI
StyI XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AleI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BanI BbeI BcgI BfrBI BmgBI BsaAI BsaBI BsaHI
BsiEI BsiWI BsmI BsmBI BspHI BspMI BsrFI BsrGI
BssHII BstBI BstEII BstZ17I Bsu36I BtgZI CspCI DraI
DraIII DrdI EagI EciI EcoRI EcoRV FalI FseI
FspI FspAI HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI NaeI NarI NdeI NgoMIV NotI NruI
NsiI PacI PciI PmeI PmlI PpiI PsiI PsrI
PvuI RsrII SacII SanDI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
SwaI TaqII TatI TstI Tth111I XbaI XmaI ZraI