(Circular) (Six-base) MAPSORT of: pFN21AB7895.seq Check: 3232 from: 1 to: 6469 pFN21AB7895 6469 bp With 182 enzymes: SgfI * April 15, 2010 09:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4279 278 Size: 53 83 186 936 2743 2468 Fragments arranged by size: 2743 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3318 3812 1937 Size: 1381 494 4594 Fragments arranged by size: 4594 1381 494 Acc65I G'GTAC_C Cuts at: 3213 3790 3213 Size: 577 5892 AclI AA'CG_TT Cuts at: 1563 4596 4969 1563 Size: 3033 373 3063 Fragments arranged by size: 3063 3033 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2327 2916 3465 3470 4532 5916 6184 1632 Size: 695 589 549 5 1062 1384 268 1917 Fragments arranged by size: 1917 1384 1062 695 589 549 268 5 AfeI AGC'GCT Cuts at: 2336 2336 Size: 6469 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6272 AflIII A'CryG_T Cuts at: 1203 3464 3580 3766 5368 6345 1203 Size: 2261 116 186 1602 977 1327 Fragments arranged by size: 2261 1602 1327 977 186 116 AgeI A'CCGG_T Cuts at: 5300 5300 Size: 6469 AhdI GACnn_n'nnGTC Cuts at: 5198 5198 Size: 6469 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6469 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6469 AlwNI CAG_nnn'CTG Cuts at: 3401 4212 5784 3401 Size: 811 1572 4086 Fragments arranged by size: 4086 1572 811 ApaLI G'TGCA_C Cuts at: 4526 5682 4526 Size: 1156 5313 ApoI r'AATT_y Cuts at: 1493 3529 3557 3778 4004 4156 6265 1493 Size: 2036 28 221 226 152 2109 1697 Fragments arranged by size: 2109 2036 1697 226 221 152 28 AseI AT'TA_AT Cuts at: 160 1032 5023 160 Size: 872 3991 1606 Fragments arranged by size: 3991 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6469 AvaI C'yCGr_G Cuts at: 1870 1943 2012 3794 1870 Size: 73 69 1782 4545 Fragments arranged by size: 4545 1782 73 69 BamHI G'GATC_C Cuts at: 3799 4166 3799 Size: 367 6102 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3213 3790 5245 618 Size: 325 217 627 1426 577 1455 1842 Fragments arranged by size: 1842 1455 1426 627 577 325 217 BanII G_rGCy'C Cuts at: 1391 3788 1391 Size: 2397 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2904 928 Size: 971 1005 4493 Fragments arranged by size: 4493 1005 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3367 3401 4660 4694 1903 Size: 34 1430 34 1259 34 3678 Fragments arranged by size: 3678 1430 1259 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4361 5571 4361 Size: 1210 5259 BclI T'GATC_A Cuts at: 1548 1548 Size: 6469 BfrBI ATG'CAT Cuts at: 2968 2968 Size: 6469 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3047 5080 136 Size: 107 122 71 631 605 1375 2033 1525 Fragments arranged by size: 2033 1525 1375 631 605 122 107 71 BglII A'GATC_T Cuts at: 2701 6464 2701 Size: 3763 2706 BlpI GC'TnA_GC Cuts at: 3886 3886 Size: 6469 Bme1580I G_kGCm'C Cuts at: 1790 4530 5686 1790 Size: 2740 1156 2573 Fragments arranged by size: 2740 2573 1156 BmgBI CAC'GTC Cuts at: 1318 2786 1318 Size: 1468 5001 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2369 2443 4200 Size: 416 53 83 399 24 952 74 1757 Cuts at: 4200 5158 442 Size: 958 1753 Fragments arranged by size: 1757 1753 958 952 416 399 83 74 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6469 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 5129 1239 Size: 3890 2579 Bpu10I CC'TnA_GC Cuts at: 2448 2448 Size: 6469 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4594 5459 5757 4594 Size: 865 298 5306 Fragments arranged by size: 5306 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5132 882 Size: 617 3633 2219 Fragments arranged by size: 3633 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2986 4260 493 Size: 711 1782 1274 2702 Fragments arranged by size: 2702 1782 1274 711 BsaBI GATnn'nnATC Cuts at: 6463 6463 Size: 6469 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4276 4658 5293 275 Size: 53 83 186 936 2743 382 635 1451 Fragments arranged by size: 2743 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4902 5300 5574 5721 6132 4902 Size: 398 274 147 411 5239 Fragments arranged by size: 5239 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2489 2519 1178 Size: 30 165 30 1086 30 5128 Fragments arranged by size: 5128 1086 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2030 1186 Size: 844 5625 BseYI C'CCAG_C Cuts at: 2217 5672 6168 2217 Size: 3455 496 2518 Fragments arranged by size: 3455 2518 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5896 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3919 4680 5708 1540 Size: 275 186 1918 761 1028 2301 Fragments arranged by size: 2301 1918 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4530 4615 5686 4530 Size: 85 1071 5313 Fragments arranged by size: 5313 1071 85 BsmI GAATG_Cn' Cuts at: 3980 4073 3980 Size: 93 6376 BsmBI CGTCTCn'nnnn_ Cuts at: 3754 3754 Size: 6469 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3788 4530 4615 5686 1391 Size: 399 1998 742 85 1071 2174 Fragments arranged by size: 2174 1998 1071 742 399 85 BspEI T'CCGG_A Cuts at: 6132 6132 Size: 6469 BspHI T'CATG_A Cuts at: 4358 6088 4358 Size: 1730 4739 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3824 844 Size: 2980 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2495 4356 1394 Size: 429 672 1861 3507 Fragments arranged by size: 3507 1861 672 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4964 5138 65 Size: 1183 3716 174 1396 Fragments arranged by size: 3716 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5113 5300 1700 Size: 110 3303 187 2869 Fragments arranged by size: 3303 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3070 3575 96 Size: 2974 505 2990 Fragments arranged by size: 2990 2974 505 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6469 BssSI C'ACGA_G Cuts at: 4529 5541 4529 Size: 1012 5457 BstAPI GCAn_nnn'nTGC Cuts at: 794 2103 794 Size: 1309 5160 BstBI TT'CG_AA Cuts at: 5273 5273 Size: 6469 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6469 BstYI r'GATC_y Cuts at: 1972 2576 2701 3746 3799 4166 4551 4568 6020 Size: 604 125 1045 53 367 385 17 1452 Cuts at: 6020 6106 6118 6464 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1045 604 385 367 346 125 86 53 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3191 3472 513 Size: 552 939 1187 281 3510 Fragments arranged by size: 3510 1187 939 552 281 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5730 Fragments arranged by size: 5730 683 56 BtsI GCAGTG_nn' Cuts at: 3515 3985 4779 4799 3515 Size: 470 794 20 5185 Fragments arranged by size: 5185 794 470 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6469 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6434 DraI TTT'AAA Cuts at: 2032 3773 2032 Size: 1741 4728 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5476 809 Size: 507 4160 1802 Fragments arranged by size: 4160 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3916 4805 6434 Size: 54 1425 50 275 18 2086 889 1629 Cuts at: 6434 8 Size: 43 Fragments arranged by size: 2086 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3916 1537 Size: 275 2104 4090 Fragments arranged by size: 4090 2104 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2019 2342 2715 3441 3644 4399 1353 Size: 666 323 373 726 203 755 3423 Fragments arranged by size: 3423 755 726 666 373 323 203 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2036 5056 5430 5576 387 Size: 1064 138 447 3020 374 146 1280 Fragments arranged by size: 3020 1280 1064 447 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2327 2916 3465 3470 4532 5129 5916 Size: 393 695 589 549 5 1062 597 787 Cuts at: 5916 6184 1239 Size: 268 1524 Fragments arranged by size: 1524 1062 787 695 597 589 549 393 268 5 EcoNI CCTnn'n_nnAGG Cuts at: 2015 2015 Size: 6469 EcoRI G'AATT_C Cuts at: 3529 3778 4156 3529 Size: 249 378 5842 Fragments arranged by size: 5842 378 249 EcoRV GAT'ATC Cuts at: 2126 2126 Size: 6469 FspI TGC'GCA Cuts at: 4975 4975 Size: 6469 HaeII r_GCGC'y Cuts at: 1678 2338 5616 1678 Size: 660 3278 2531 Fragments arranged by size: 3278 2531 660 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2489 2521 3016 3048 5110 Size: 32 135 32 917 32 495 32 2062 Cuts at: 5110 5142 5184 5216 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2062 917 495 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2950 3319 3813 4059 669 Size: 988 281 1012 369 494 246 3079 Fragments arranged by size: 3079 1012 988 494 369 281 246 HindIII A'AGCT_T Cuts at: 748 3231 748 Size: 2483 3986 HpaI GTT'AAC Cuts at: 4059 4059 Size: 6469 KpnI G_GTAC'C Cuts at: 3217 3794 3217 Size: 577 5892 MfeI C'AATT_G Cuts at: 4068 4068 Size: 6469 MluI A'CGCG_T Cuts at: 6345 6345 Size: 6469 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2836 5583 5767 1305 Size: 1531 2747 184 2007 Fragments arranged by size: 2747 2007 1531 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4647 Fragments arranged by size: 4647 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3092 4427 4786 4945 518 Size: 648 1926 1335 359 159 2042 Fragments arranged by size: 2042 1926 1335 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2469 3885 4562 5710 5955 1606 Size: 863 1416 677 1148 245 2120 Fragments arranged by size: 2120 1416 1148 863 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 3191 3472 513 Size: 552 939 1187 281 3510 Fragments arranged by size: 3510 1187 939 552 281 NdeI CA'TA_TG Cuts at: 387 387 Size: 6469 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6469 NotI GC'GGCC_GC Cuts at: 3916 3916 Size: 6469 NsiI A_TGCA'T Cuts at: 2970 2970 Size: 6469 NspI r_CATG'y Cuts at: 1132 1229 3584 3770 3827 5372 1132 Size: 97 2355 186 57 1545 2229 Fragments arranged by size: 2355 2229 1545 186 97 57 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6469 PciI A'CATG_T Cuts at: 3580 3766 5368 3580 Size: 186 1602 4681 Fragments arranged by size: 4681 1602 186 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6469 PmeI GTTT'AAAC Cuts at: 3773 3773 Size: 6469 PmlI CAC'GTG Cuts at: 2986 2986 Size: 6469 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4538 4570 6051 6083 4538 Size: 32 1481 32 4924 Fragments arranged by size: 4924 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6469 PsiI TTA'TAA Cuts at: 4039 4039 Size: 6469 PspXI vC'TCGA_Gb Cuts at: 2012 2012 Size: 6469 PstI C_TGCA'G Cuts at: 830 3566 3821 830 Size: 2736 255 3478 Fragments arranged by size: 3478 2736 255 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6469 PvuII CAG'CTG Cuts at: 2469 2469 Size: 6469 SalI G'TCGA_C Cuts at: 1936 3317 3811 1936 Size: 1381 494 4594 Fragments arranged by size: 4594 1381 494 SapI GCTCTTCn'nnn_ Cuts at: 2342 2342 Size: 6469 SbfI CC_TGCA'GG Cuts at: 3821 3821 Size: 6469 ScaI AGT'ACT Cuts at: 4717 6429 4717 Size: 1712 4757 SfcI C'TryA_G Cuts at: 826 1045 3036 3078 3362 3562 3817 4952 5633 Size: 219 1991 42 284 200 255 1135 681 Cuts at: 5633 5824 826 Size: 191 1471 Fragments arranged by size: 1991 1471 1135 681 284 255 219 200 191 42 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6469 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6469 SmaI CCC'GGG Cuts at: 1872 3796 1872 Size: 1924 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2012 4573 5474 5736 820 Size: 197 926 69 2561 901 262 1553 Fragments arranged by size: 2561 1553 926 901 262 197 69 SnaBI TAC'GTA Cuts at: 493 4260 493 Size: 3767 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6469 SphI G_CATG'C Cuts at: 1132 3827 1132 Size: 2695 3774 SspI AAT'ATT Cuts at: 5 52 2090 4393 5 Size: 47 2038 2303 2081 Fragments arranged by size: 2303 2081 2038 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6469 StyI C'CwwG_G Cuts at: 513 1065 2004 2284 3191 3472 3908 513 Size: 552 939 280 907 281 436 3074 Fragments arranged by size: 3074 939 907 552 436 281 280 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4496 4513 4666 4851 5296 325 Size: 1255 2916 17 153 185 445 1498 Fragments arranged by size: 2916 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2042 3070 3548 Size: 274 80 33 51 1443 65 1028 478 Cuts at: 3548 3575 4715 6427 96 Size: 27 1140 1712 138 Fragments arranged by size: 1712 1443 1140 1028 478 274 138 80 65 51 33 27 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6429 6461 6429 Size: 32 6437 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6469 XbaI T'CTAG_A Cuts at: 3805 3805 Size: 6469 XhoI C'TCGA_G Cuts at: 1943 2012 1943 Size: 69 6400 XmaI C'CCGG_G Cuts at: 1870 3794 1870 Size: 1924 4545 XmnI GAAnn'nnTTC Cuts at: 4598 4598 Size: 6469 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4277 276 Size: 53 83 186 936 2743 2468 Fragments arranged by size: 2743 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspXI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoO109I FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PpuMI PspOMI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI