(Circular) (Six-base) MAPSORT of: pFN21AB5798.seq Check: 7664 from: 1 to: 7048 pFN21AB5798 7048 bp With 182 enzymes: SgfI * April 15, 2010 09:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3397 3397 Size: 7048 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4858 278 Size: 53 83 186 936 3322 2468 Fragments arranged by size: 3322 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4391 1937 Size: 2454 4594 Acc65I G'GTAC_C Cuts at: 2978 4369 2978 Size: 1391 5657 AclI AA'CG_TT Cuts at: 1563 5175 5548 1563 Size: 3612 373 3063 Fragments arranged by size: 3612 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2378 2897 3161 3314 3390 3398 3875 4255 Size: 746 519 264 153 76 8 477 380 Cuts at: 4255 5111 6495 6763 1632 Size: 856 1384 268 1917 Fragments arranged by size: 1917 1384 856 746 519 477 380 268 264 153 76 8 AfeI AGC'GCT Cuts at: 3375 3375 Size: 7048 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6851 AflIII A'CryG_T Cuts at: 1203 4283 5947 6924 1203 Size: 3080 1664 977 1327 Fragments arranged by size: 3080 1664 1327 977 AgeI A'CCGG_T Cuts at: 5879 5879 Size: 7048 AhdI GACnn_n'nnGTC Cuts at: 4320 5777 4320 Size: 1457 5591 AleI CACnn'nnGTG Cuts at: 1166 2099 1166 Size: 933 6115 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7048 AlwNI CAG_nnn'CTG Cuts at: 2224 2245 2650 3020 3118 3629 4791 6363 2224 Size: 21 405 370 98 511 1162 1572 2909 Fragments arranged by size: 2909 1572 1162 511 405 370 98 21 ApaI G_GGCC'C Cuts at: 4257 4257 Size: 7048 ApaLI G'TGCA_C Cuts at: 5105 6261 5105 Size: 1156 5892 ApoI r'AATT_y Cuts at: 1493 2778 3270 4357 4583 4735 6844 1493 Size: 1285 492 1087 226 152 2109 1697 Fragments arranged by size: 2109 1697 1285 1087 492 226 152 AseI AT'TA_AT Cuts at: 160 1032 5602 160 Size: 872 4570 1606 Fragments arranged by size: 4570 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7048 AvaI C'yCGr_G Cuts at: 1870 1943 2375 2444 2665 2707 2835 3008 3094 Size: 73 432 69 221 42 128 173 86 Cuts at: 3094 4373 1870 Size: 1279 4545 Fragments arranged by size: 4545 1279 432 221 173 128 86 73 69 42 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2970 3003 3492 3525 3904 3937 2970 Size: 33 489 33 379 33 6081 Fragments arranged by size: 6081 489 379 33 33 33 BamHI G'GATC_C Cuts at: 2121 3999 4378 4745 2121 Size: 1878 379 367 4424 Fragments arranged by size: 4424 1878 379 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2053 2660 2761 2978 3024 Size: 325 217 627 266 607 101 217 46 Cuts at: 3024 3066 4369 5824 618 Size: 42 1303 1455 1842 Fragments arranged by size: 1842 1455 1303 627 607 325 266 217 217 101 46 42 BanII G_rGCy'C Cuts at: 1391 2262 2543 3095 3183 3938 4257 4367 1391 Size: 871 281 552 88 755 319 110 4072 Fragments arranged by size: 4072 871 755 552 319 281 110 88 BbeI G_GCGC'C Cuts at: 2664 2765 2664 Size: 101 6947 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2366 928 Size: 971 467 5610 Fragments arranged by size: 5610 971 467 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2671 2705 4111 4145 5239 5273 1903 Size: 34 734 34 1406 34 1094 34 3678 Fragments arranged by size: 3678 1406 1094 734 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3520 4940 6150 3520 Size: 1420 1210 4418 Fragments arranged by size: 4418 1420 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 7048 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5659 136 Size: 107 122 71 631 605 3987 1525 Fragments arranged by size: 3987 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7043 7043 Size: 7048 BlpI GC'TnA_GC Cuts at: 4465 4465 Size: 7048 Bme1580I G_kGCm'C Cuts at: 1790 2058 3027 4257 5109 6265 1790 Size: 268 969 1230 852 1156 2573 Fragments arranged by size: 2573 1230 1156 969 852 268 BmgBI CAC'GTC Cuts at: 1318 3463 1318 Size: 2145 4903 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2963 3976 4078 Size: 416 53 83 399 24 1546 1013 102 Cuts at: 4078 4779 5737 442 Size: 701 958 1753 Fragments arranged by size: 1753 1546 1013 958 701 416 399 102 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7048 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2515 2515 Size: 7048 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2063 2101 2349 3305 3422 3443 3476 3572 Size: 824 38 248 956 117 21 33 96 Cuts at: 3572 5708 1239 Size: 2136 2579 Fragments arranged by size: 2579 2136 956 824 248 117 96 38 33 21 Bpu10I CC'TnA_GC Cuts at: 2477 3104 3783 2477 Size: 627 679 5742 Fragments arranged by size: 5742 679 627 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2753 3639 5173 6038 6336 2753 Size: 886 1534 865 298 3465 Fragments arranged by size: 3465 1534 886 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2958 5711 882 Size: 617 1459 2753 2219 Fragments arranged by size: 2753 2219 1459 617 BsaAI yAC'GTr Cuts at: 493 1204 4286 4839 493 Size: 711 3082 553 2702 Fragments arranged by size: 3082 2702 711 553 BsaBI GATnn'nnATC Cuts at: 7042 7042 Size: 7048 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2661 2762 4106 4855 Size: 53 83 186 936 1128 101 1344 749 Cuts at: 4855 5237 5872 275 Size: 382 635 1451 Fragments arranged by size: 1451 1344 1128 936 749 635 382 186 101 83 53 BsaWI w'CCGG_w Cuts at: 2851 2912 5481 5879 6153 6300 6711 2851 Size: 61 2569 398 274 147 411 3188 Fragments arranged by size: 3188 2569 411 398 274 147 61 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2612 2642 2955 2985 3395 Size: 30 165 30 1209 30 313 30 410 Cuts at: 3395 3425 3554 3584 1178 Size: 30 129 30 4642 Fragments arranged by size: 4642 1209 410 313 165 129 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2060 2270 3257 3332 3353 3642 3671 4299 Size: 874 210 987 75 21 289 29 628 Cuts at: 4299 1186 Size: 3935 Fragments arranged by size: 3935 987 874 628 289 210 75 29 21 BseYI C'CCAG_C Cuts at: 3111 6251 6747 3111 Size: 3140 496 3412 Fragments arranged by size: 3412 3140 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2123 2225 2455 2701 3410 1163 Size: 573 387 102 230 246 709 4801 Fragments arranged by size: 4801 709 573 387 246 230 102 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2405 4498 5259 6287 1540 Size: 275 186 404 2093 761 1028 2301 Fragments arranged by size: 2301 2093 1028 761 404 275 186 BsiHKAI G_wGCw'C Cuts at: 2115 2262 3216 3716 5109 5194 6265 2115 Size: 147 954 500 1393 85 1071 2898 Fragments arranged by size: 2898 1393 1071 954 500 147 85 BsiWI C'GTAC_G Cuts at: 2406 2406 Size: 7048 BsmI GAATG_Cn' Cuts at: 4559 4652 4559 Size: 93 6955 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2058 2115 2262 2543 3027 3095 3183 Size: 399 268 57 147 281 484 68 88 Cuts at: 3183 3216 3716 3938 4257 4367 5109 5194 6265 Size: 33 500 222 319 110 742 85 1071 Cuts at: 6265 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 500 484 399 319 281 268 222 147 110 88 85 68 57 33 BspEI T'CCGG_A Cuts at: 2851 6711 2851 Size: 3860 3188 BspHI T'CATG_A Cuts at: 4937 6667 4937 Size: 1730 5318 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2209 2709 3136 3397 4403 844 Size: 1365 500 427 261 1006 3489 Fragments arranged by size: 3489 1365 1006 500 427 261 BsrBI CCG'CTC Cuts at: 1394 1823 3048 4935 1394 Size: 429 1225 1887 3507 Fragments arranged by size: 3507 1887 1225 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5543 5717 65 Size: 1183 4295 174 1396 Fragments arranged by size: 4295 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2152 5692 5879 1700 Size: 110 342 3540 187 2869 Fragments arranged by size: 3540 2869 342 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7048 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7048 BssSI C'ACGA_G Cuts at: 5108 6120 5108 Size: 1012 6036 BstAPI GCAn_nnn'nTGC Cuts at: 794 2224 3388 794 Size: 1430 1164 4454 Fragments arranged by size: 4454 1430 1164 BstBI TT'CG_AA Cuts at: 2691 5852 2691 Size: 3161 3887 BstEII G'GTnAC_C Cuts at: 1184 2176 2737 2908 1184 Size: 992 561 171 5324 Fragments arranged by size: 5324 992 561 171 BstXI CCAn_nnnn'nTGG Cuts at: 2173 2332 3476 4156 2173 Size: 159 1144 680 5065 Fragments arranged by size: 5065 1144 680 159 BstYI r'GATC_y Cuts at: 1972 2121 2136 3672 3999 4214 4378 4745 5130 Size: 149 15 1536 327 215 164 367 385 Cuts at: 5130 5147 6599 6685 6697 7043 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1536 1452 385 367 346 327 215 164 149 86 17 15 12 Bsu36I CC'TnA_GG Cuts at: 3037 3037 Size: 7048 BtgI C'CryG_G Cuts at: 513 1065 2004 2631 513 Size: 552 939 627 4930 Fragments arranged by size: 4930 939 627 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3505 487 Size: 683 56 2279 4030 Fragments arranged by size: 4030 2279 683 56 BtsI GCAGTG_nn' Cuts at: 3354 4564 5358 5378 3354 Size: 1210 794 20 5024 Fragments arranged by size: 5024 1210 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7048 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7013 DraI TTT'AAA Cuts at: 4352 4352 Size: 7048 DraIII CAC_nnn'GTG Cuts at: 3229 3229 Size: 7048 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6055 809 Size: 507 4739 1802 Fragments arranged by size: 4739 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2163 2250 2402 Size: 54 1425 50 275 18 333 87 152 Cuts at: 2402 3955 4495 5384 7013 8 Size: 1553 540 889 1629 43 Fragments arranged by size: 1629 1553 1425 889 540 333 275 152 87 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2402 4495 1537 Size: 275 590 2093 4090 Fragments arranged by size: 4090 2093 590 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2187 3083 3164 3704 3851 4978 1353 Size: 834 896 81 540 147 1127 3423 Fragments arranged by size: 3423 1127 896 834 540 147 81 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2430 5635 6009 6155 387 Size: 1064 138 841 3205 374 146 1280 Fragments arranged by size: 3205 1280 1064 841 374 146 138 EcoICRI GAG'CTC Cuts at: 2260 2260 Size: 7048 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2063 2101 2349 2378 2897 3161 3305 Size: 393 431 38 248 29 519 264 144 Cuts at: 3305 3314 3390 3398 3422 3443 3476 3572 3875 Size: 9 76 8 24 21 33 96 303 Cuts at: 3875 4255 5111 5708 6495 6763 1239 Size: 380 856 597 787 268 1524 Fragments arranged by size: 1524 856 787 597 519 431 393 380 303 268 264 248 144 96 76 38 33 29 24 21 9 8 EcoNI CCTnn'n_nnAGG Cuts at: 3143 3143 Size: 7048 EcoO109I rG'GnC_Cy Cuts at: 2221 2506 2685 2885 4254 4340 2221 Size: 285 179 200 1369 86 4929 Fragments arranged by size: 4929 1369 285 200 179 86 EcoRI G'AATT_C Cuts at: 4357 4735 4357 Size: 378 6670 FspI TGC'GCA Cuts at: 2615 5554 2615 Size: 2939 4109 HaeII r_GCGC'y Cuts at: 1678 2245 2585 2664 2765 3136 3377 3783 6195 Size: 567 340 79 101 371 241 406 2412 Cuts at: 6195 1678 Size: 2531 Fragments arranged by size: 2531 2412 567 406 371 340 241 101 79 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2152 2184 3311 3343 3554 Size: 32 135 32 580 32 1127 32 211 Cuts at: 3554 3586 3637 3669 3766 3798 4206 4238 4306 Size: 32 51 32 97 32 408 32 68 Cuts at: 4306 4338 5689 5721 5763 5795 1373 Size: 32 1351 32 42 32 2626 Fragments arranged by size: 2626 1351 1127 580 408 211 135 97 68 51 42 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2974 4392 4638 669 Size: 988 281 1036 1418 246 3079 Fragments arranged by size: 3079 1418 1036 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7048 HpaI GTT'AAC Cuts at: 4638 4638 Size: 7048 KasI G'GCGC_C Cuts at: 2660 2761 2660 Size: 101 6947 KpnI G_GTAC'C Cuts at: 2982 4373 2982 Size: 1391 5657 MfeI C'AATT_G Cuts at: 4647 4647 Size: 7048 MluI A'CGCG_T Cuts at: 6924 6924 Size: 7048 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3700 3961 4162 6162 6346 1305 Size: 2395 261 201 2000 184 2007 Fragments arranged by size: 2395 2007 2000 261 201 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2165 10 Size: 54 1425 343 333 4893 Fragments arranged by size: 4893 1425 343 333 54 MslI CAynn'nnrTG Cuts at: 518 1166 2099 4166 5006 5365 5524 518 Size: 648 933 2067 840 359 159 2042 Fragments arranged by size: 2067 2042 933 840 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2633 2807 2946 3154 3283 3385 3918 4239 Size: 1027 174 139 208 129 102 533 321 Cuts at: 4239 4464 5141 6289 6534 1606 Size: 225 677 1148 245 2120 Fragments arranged by size: 2120 1148 1027 677 533 321 245 225 208 174 139 129 102 NarI GG'CG_CC Cuts at: 2661 2762 2661 Size: 101 6947 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5557 Fragments arranged by size: 5557 939 552 NdeI CA'TA_TG Cuts at: 387 3496 387 Size: 3109 3939 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7048 NotI GC'GGCC_GC Cuts at: 4495 4495 Size: 7048 NspI r_CATG'y Cuts at: 1132 1229 3794 4406 5951 1132 Size: 97 2565 612 1545 2229 Fragments arranged by size: 2565 2229 1545 612 97 PasI CC'CwG_GG Cuts at: 1348 2332 4277 1348 Size: 984 1945 4119 Fragments arranged by size: 4119 1945 984 PciI A'CATG_T Cuts at: 5947 5947 Size: 7048 PflMI CCAn_nnn'nTGG Cuts at: 1108 4061 4331 1108 Size: 2953 270 3825 Fragments arranged by size: 3825 2953 270 PfoI T'CCnGG_A Cuts at: 2117 3420 2117 Size: 1303 5745 PmeI GTTT'AAAC Cuts at: 4352 4352 Size: 7048 PmlI CAC'GTG Cuts at: 4286 4286 Size: 7048 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5117 5149 6630 6662 5117 Size: 32 1481 32 5503 Fragments arranged by size: 5503 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2221 2221 Size: 7048 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7048 PsiI TTA'TAA Cuts at: 4618 4618 Size: 7048 PspOMI G'GGCC_C Cuts at: 4253 4253 Size: 7048 PstI C_TGCA'G Cuts at: 830 2091 2220 2579 2646 3002 3200 3591 4400 Size: 1261 129 359 67 356 198 391 809 Cuts at: 4400 830 Size: 3478 Fragments arranged by size: 3478 1261 809 391 359 356 198 129 67 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7048 PvuII CAG'CTG Cuts at: 2807 3154 3283 3918 4239 2807 Size: 347 129 635 321 5616 Fragments arranged by size: 5616 635 347 321 129 SacI G_AGCT'C Cuts at: 2262 2262 Size: 7048 SacII CC_GC'GG Cuts at: 2634 2634 Size: 7048 SalI G'TCGA_C Cuts at: 1936 4390 1936 Size: 2454 4594 SapI GCTCTTCn'nnn_ Cuts at: 3083 3164 3704 3083 Size: 81 540 6427 Fragments arranged by size: 6427 540 81 SbfI CC_TGCA'GG Cuts at: 2220 2579 4400 2220 Size: 359 1821 4868 Fragments arranged by size: 4868 1821 359 ScaI AGT'ACT Cuts at: 3722 5296 7008 3722 Size: 1574 1712 3762 Fragments arranged by size: 3762 1712 1574 SfcI C'TryA_G Cuts at: 826 1045 2087 2216 2575 2642 2748 2998 3196 Size: 219 1042 129 359 67 106 250 198 Cuts at: 3196 3587 4174 4396 5531 6212 6403 826 Size: 391 587 222 1135 681 191 1471 Fragments arranged by size: 1471 1135 1042 681 587 391 359 250 222 219 198 191 129 106 67 SfoI GGC'GCC Cuts at: 2662 2763 2662 Size: 101 6947 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7048 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7048 SmaI CCC'GGG Cuts at: 1872 2667 2837 3096 4375 1872 Size: 795 170 259 1279 4545 Fragments arranged by size: 4545 1279 795 259 170 SmlI C'TyrA_G Cuts at: 820 1017 1943 2732 3654 5152 6053 6315 820 Size: 197 926 789 922 1498 901 262 1553 Fragments arranged by size: 1553 1498 926 922 901 789 262 197 SnaBI TAC'GTA Cuts at: 493 4839 493 Size: 4346 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7048 SphI G_CATG'C Cuts at: 1132 4406 1132 Size: 3274 3774 SspI AAT'ATT Cuts at: 5 52 2285 4972 5 Size: 47 2233 2687 2081 Fragments arranged by size: 2687 2233 2081 47 StuI AGG'CCT Cuts at: 1517 2498 3035 1517 Size: 981 537 5530 Fragments arranged by size: 5530 981 537 StyI C'CwwG_G Cuts at: 513 1065 2004 3217 3558 4487 513 Size: 552 939 1213 341 929 3074 Fragments arranged by size: 3074 1213 939 929 552 341 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2896 4060 4319 5075 5092 5245 5430 Size: 1255 1316 1164 259 756 17 153 185 Cuts at: 5430 5875 325 Size: 445 1498 Fragments arranged by size: 1498 1316 1255 1164 756 445 259 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3411 3720 5294 Size: 274 80 33 51 1443 1434 309 1574 Cuts at: 5294 7006 96 Size: 1712 138 Fragments arranged by size: 1712 1574 1443 1434 309 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7008 7040 7008 Size: 32 7016 Tth111I GACn'n_nGTC Cuts at: 1316 4048 1316 Size: 2732 4316 XbaI T'CTAG_A Cuts at: 2791 4384 2791 Size: 1593 5455 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7048 XmaI C'CCGG_G Cuts at: 1870 2665 2835 3094 4373 1870 Size: 795 170 259 1279 4545 Fragments arranged by size: 4545 1279 795 259 170 XmnI GAAnn'nnTTC Cuts at: 5177 5177 Size: 7048 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4856 276 Size: 53 83 186 936 3322 2468 Fragments arranged by size: 3322 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BbvCI BfrBI BsmBI BstZ17I EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PspXI PsrI RsrII SanDI SexAI SfiI SrfI SwaI XcmI