(Circular) (Six-base) MAPSORT of: pFN21AB7784.seq Check: 1650 from: 1 to: 6235 pFN21AB7784 6235 bp With 182 enzymes: SgfI * April 15, 2010 09:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4045 278 Size: 53 83 186 936 2509 2468 Fragments arranged by size: 2509 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3578 1937 Size: 1641 4594 Acc65I G'GTAC_C Cuts at: 3556 3556 Size: 6235 AclI AA'CG_TT Cuts at: 1563 3534 4362 4735 1563 Size: 1971 828 373 3063 Fragments arranged by size: 3063 1971 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2198 3109 4298 5682 5950 1632 Size: 566 911 1189 1384 268 1917 Fragments arranged by size: 1917 1384 1189 911 566 268 AfeI AGC'GCT Cuts at: 2146 2146 Size: 6235 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6038 AflIII A'CryG_T Cuts at: 1203 3437 5134 6111 1203 Size: 2234 1697 977 1327 Fragments arranged by size: 2234 1697 1327 977 AgeI A'CCGG_T Cuts at: 5066 5066 Size: 6235 AhdI GACnn_n'nnGTC Cuts at: 4964 4964 Size: 6235 AleI CACnn'nnGTG Cuts at: 1166 3420 1166 Size: 2254 3981 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6235 AlwNI CAG_nnn'CTG Cuts at: 2236 2296 2365 3978 5550 2236 Size: 60 69 1613 1572 2921 Fragments arranged by size: 2921 1613 1572 69 60 ApaLI G'TGCA_C Cuts at: 4292 5448 4292 Size: 1156 5079 ApoI r'AATT_y Cuts at: 1493 2378 3159 3327 3544 3770 3922 6031 1493 Size: 885 781 168 217 226 152 2109 1697 Fragments arranged by size: 2109 1697 885 781 226 217 168 152 AseI AT'TA_AT Cuts at: 160 1032 4789 160 Size: 872 3757 1606 Fragments arranged by size: 3757 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6235 AvaI C'yCGr_G Cuts at: 1870 1943 3560 1870 Size: 73 1617 4545 Fragments arranged by size: 4545 1617 73 BamHI G'GATC_C Cuts at: 3565 3932 3565 Size: 367 5868 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3556 5011 618 Size: 325 217 627 1769 1455 1842 Fragments arranged by size: 1842 1769 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2502 3554 1391 Size: 1111 1052 4072 Fragments arranged by size: 4072 1111 1052 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2780 928 Size: 971 881 4383 Fragments arranged by size: 4383 971 881 BbvCI CC'TCA_GC Cuts at: 2237 2237 Size: 6235 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4426 4460 1903 Size: 34 2489 34 3678 Fragments arranged by size: 3678 2489 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4127 5337 4127 Size: 1210 5025 BclI T'GATC_A Cuts at: 1548 2617 2857 1548 Size: 1069 240 4926 Fragments arranged by size: 4926 1069 240 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4846 136 Size: 107 122 71 631 605 3174 1525 Fragments arranged by size: 3174 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2265 3223 6230 2265 Size: 958 3007 2270 Fragments arranged by size: 3007 2270 958 BlpI GC'TnA_GC Cuts at: 3652 3652 Size: 6235 Bme1580I G_kGCm'C Cuts at: 1790 4296 5452 1790 Size: 2506 1156 2573 Fragments arranged by size: 2573 2506 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6235 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2335 3966 4924 Size: 416 53 83 399 24 918 1631 958 Cuts at: 4924 442 Size: 1753 Fragments arranged by size: 1753 1631 958 918 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6235 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2269 2269 Size: 6235 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2077 2182 3189 4895 1239 Size: 838 105 1007 1706 2579 Fragments arranged by size: 2579 1706 1007 838 105 Bpu10I CC'TnA_GC Cuts at: 2237 2297 2237 Size: 60 6175 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2023 2216 2789 4360 5225 5523 2023 Size: 193 573 1571 865 298 2735 Fragments arranged by size: 2735 1571 865 573 298 193 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2309 4898 882 Size: 617 810 2589 2219 Fragments arranged by size: 2589 2219 810 617 BsaAI yAC'GTr Cuts at: 493 1204 4026 493 Size: 711 2822 2702 Fragments arranged by size: 2822 2702 711 BsaBI GATnn'nnATC Cuts at: 6229 6229 Size: 6235 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4042 4424 5059 275 Size: 53 83 186 936 2509 382 635 1451 Fragments arranged by size: 2509 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2186 4668 5066 5340 5487 5898 2186 Size: 2482 398 274 147 411 2523 Fragments arranged by size: 2523 2482 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2155 2185 1178 Size: 30 165 30 752 30 5228 Fragments arranged by size: 5228 752 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6235 BseYI C'CCAG_C Cuts at: 2431 2563 2589 5438 5934 2431 Size: 132 26 2849 496 2732 Fragments arranged by size: 2849 2732 496 132 26 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2351 1163 Size: 573 615 5047 Fragments arranged by size: 5047 615 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3473 3685 4446 5474 1540 Size: 275 186 1472 212 761 1028 2301 Fragments arranged by size: 2301 1472 1028 761 275 212 186 BsiHKAI G_wGCw'C Cuts at: 2451 4296 4381 5452 2451 Size: 1845 85 1071 3234 Fragments arranged by size: 3234 1845 1071 85 BsmI GAATG_Cn' Cuts at: 2301 3746 3839 2301 Size: 1445 93 4697 Fragments arranged by size: 4697 1445 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2451 2502 3554 4296 4381 5452 1391 Size: 399 661 51 1052 742 85 1071 2174 Fragments arranged by size: 2174 1071 1052 742 661 399 85 51 BspEI T'CCGG_A Cuts at: 5898 5898 Size: 6235 BspHI T'CATG_A Cuts at: 2176 3240 4124 5854 2176 Size: 1064 884 1730 2557 Fragments arranged by size: 2557 1730 1064 884 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3034 3590 844 Size: 2190 556 3489 Fragments arranged by size: 3489 2190 556 BsrBI CCG'CTC Cuts at: 1394 1823 4122 1394 Size: 429 2299 3507 Fragments arranged by size: 3507 2299 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4730 4904 65 Size: 1183 3482 174 1396 Fragments arranged by size: 3482 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4879 5066 1700 Size: 110 3069 187 2869 Fragments arranged by size: 3069 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2980 96 Size: 2884 3351 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6235 BssSI C'ACGA_G Cuts at: 4295 5307 4295 Size: 1012 5223 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6235 BstBI TT'CG_AA Cuts at: 5039 5039 Size: 6235 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6235 BstYI r'GATC_y Cuts at: 1972 2247 2265 3061 3223 3565 3932 4317 4334 Size: 275 18 796 162 342 367 385 17 Cuts at: 4334 5786 5872 5884 6230 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 796 385 367 346 342 275 162 86 18 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4744 Fragments arranged by size: 4744 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2024 487 Size: 683 56 798 4698 Fragments arranged by size: 4698 798 683 56 BtsI GCAGTG_nn' Cuts at: 2451 2472 3751 4545 4565 2451 Size: 21 1279 794 20 4121 Fragments arranged by size: 4121 1279 794 21 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6235 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6200 DraI TTT'AAA Cuts at: 3352 3539 3352 Size: 187 6048 DraIII CAC_nnn'GTG Cuts at: 2978 2978 Size: 6235 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2074 5242 809 Size: 507 758 3168 1802 Fragments arranged by size: 3168 1802 758 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3682 4571 6200 Size: 54 1425 50 275 18 1852 889 1629 Cuts at: 6200 8 Size: 43 Fragments arranged by size: 1852 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3682 1537 Size: 275 1870 4090 Fragments arranged by size: 4090 1870 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2739 4165 1353 Size: 1386 1426 3423 Fragments arranged by size: 3423 1426 1386 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4822 5196 5342 387 Size: 1064 138 3233 374 146 1280 Fragments arranged by size: 3233 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2077 2182 2198 3109 3189 4298 4895 Size: 393 445 105 16 911 80 1109 597 Cuts at: 4895 5682 5950 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1109 911 787 597 445 393 268 105 80 16 EcoO109I rG'GnC_Cy Cuts at: 3077 3077 Size: 6235 EcoRI G'AATT_C Cuts at: 2378 3159 3327 3544 3922 2378 Size: 781 168 217 378 4691 Fragments arranged by size: 4691 781 378 217 168 EcoRV GAT'ATC Cuts at: 2803 2803 Size: 6235 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3475 3475 Size: 6235 FspI TGC'GCA Cuts at: 4741 4741 Size: 6235 HaeII r_GCGC'y Cuts at: 1678 2148 5382 1678 Size: 470 3234 2531 Fragments arranged by size: 3234 2531 470 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2021 2053 3208 3240 4876 Size: 32 135 32 449 32 1155 32 1636 Cuts at: 4876 4908 4950 4982 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1636 1155 449 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2709 3191 3579 3825 669 Size: 988 281 771 482 388 246 3079 Fragments arranged by size: 3079 988 771 482 388 281 246 HindIII A'AGCT_T Cuts at: 748 3121 3289 748 Size: 2373 168 3694 Fragments arranged by size: 3694 2373 168 HpaI GTT'AAC Cuts at: 3825 3825 Size: 6235 KpnI G_GTAC'C Cuts at: 3560 3560 Size: 6235 MfeI C'AATT_G Cuts at: 3834 3834 Size: 6235 MluI A'CGCG_T Cuts at: 6111 6111 Size: 6235 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5349 5533 1305 Size: 4044 184 2007 Fragments arranged by size: 4044 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4413 Fragments arranged by size: 4413 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2899 3268 3420 4193 4552 4711 Size: 648 843 890 369 152 773 359 159 Cuts at: 4711 518 Size: 2042 Fragments arranged by size: 2042 890 843 773 648 369 359 159 152 MspA1I CmG'CkG Cuts at: 1606 2563 3651 4328 5476 5721 1606 Size: 957 1088 677 1148 245 2120 Fragments arranged by size: 2120 1148 1088 957 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4744 Fragments arranged by size: 4744 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6235 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6235 NotI GC'GGCC_GC Cuts at: 3682 3682 Size: 6235 NspI r_CATG'y Cuts at: 1132 1229 3441 3519 3593 5138 1132 Size: 97 2212 78 74 1545 2229 Fragments arranged by size: 2229 2212 1545 97 78 74 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6235 PciI A'CATG_T Cuts at: 3437 5134 3437 Size: 1697 4538 PflMI CCAn_nnn'nTGG Cuts at: 1108 2431 2509 1108 Size: 1323 78 4834 Fragments arranged by size: 4834 1323 78 PmeI GTTT'AAAC Cuts at: 3539 3539 Size: 6235 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4304 4336 5817 5849 4304 Size: 32 1481 32 4690 Fragments arranged by size: 4690 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6235 PsiI TTA'TAA Cuts at: 3805 3805 Size: 6235 PstI C_TGCA'G Cuts at: 830 3587 830 Size: 2757 3478 PvuI CG_AT'CG Cuts at: 2001 3473 2001 Size: 1472 4763 PvuII CAG'CTG Cuts at: 2563 2563 Size: 6235 RsrII CG'GwC_CG Cuts at: 2189 2189 Size: 6235 SalI G'TCGA_C Cuts at: 1936 3577 1936 Size: 1641 4594 SbfI CC_TGCA'GG Cuts at: 3587 3587 Size: 6235 ScaI AGT'ACT Cuts at: 4483 6195 4483 Size: 1712 4523 SfcI C'TryA_G Cuts at: 826 1045 3583 4718 5399 5590 826 Size: 219 2538 1135 681 191 1471 Fragments arranged by size: 2538 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6235 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6235 SmaI CCC'GGG Cuts at: 1872 3562 1872 Size: 1690 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2038 2195 2768 4339 5240 5502 Size: 197 926 95 157 573 1571 901 262 Cuts at: 5502 820 Size: 1553 Fragments arranged by size: 1571 1553 926 901 573 262 197 157 95 SnaBI TAC'GTA Cuts at: 493 4026 493 Size: 3533 2702 SpeI A'CTAG_T Cuts at: 152 2069 152 Size: 1917 4318 SphI G_CATG'C Cuts at: 1132 3593 1132 Size: 2461 3774 SspI AAT'ATT Cuts at: 5 52 2531 4159 5 Size: 47 2479 1628 2081 Fragments arranged by size: 2479 2081 1628 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6235 StyI C'CwwG_G Cuts at: 513 1065 2004 2310 3005 3674 513 Size: 552 939 306 695 669 3074 Fragments arranged by size: 3074 939 695 669 552 306 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2317 2501 4262 4279 4432 4617 5062 Size: 1255 737 184 1761 17 153 185 445 Cuts at: 5062 325 Size: 1498 Fragments arranged by size: 1761 1498 1255 737 445 185 184 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2980 4481 6193 Size: 274 80 33 51 1443 1003 1501 1712 Cuts at: 6193 96 Size: 138 Fragments arranged by size: 1712 1501 1443 1003 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2451 2483 6195 6227 2451 Size: 32 3712 32 2459 Fragments arranged by size: 3712 2459 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6235 XbaI T'CTAG_A Cuts at: 3571 3571 Size: 6235 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2481 2481 Size: 6235 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6235 XmaI C'CCGG_G Cuts at: 1870 3560 1870 Size: 1690 4545 XmnI GAAnn'nnTTC Cuts at: 3122 4364 3122 Size: 1242 4993 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4043 276 Size: 53 83 186 936 2509 2468 Fragments arranged by size: 2509 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII RsrII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI