Restriction map for
pFN21AB5777
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# NO CUT ENZYMES ( 118 enzymes) #
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AatII Acc65I AccI AccII AclI
AcyI AflIII AgeI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspI AvaI
AvrII BamHI BanI BbeI BbrPI
BbvCI BciVI BclI BcnI BglI
BlnI BsaAI BsaHI BsiEI BsiWI
BsmBI BsoBI BspDI BspLU11I BsrBI
BsrFI BssHII Bst1107I BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
Cfr10I ClaI CpoI DraII DrdI
EagI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I FbaI FseI
HgaI Hin1I HincII HindII HpaI
KasI KpnI KspI MaeII MluI
MvnI NaeI NarI NciI NdeI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RsrII SacI SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
Sse8387I SwaI Tth111I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AflII 456 CTTAAG C^TTAAG
AhdI 287 GACAAGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 287 GACAAGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 2000 GTGCTC G[AT]GC[AT]^C
AviII 627 TGCGCA TGC^GCA
BalI 1467 TGGCCA TGG^CCA
BcgI 341 GCACGCAATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 456 CTTAAG C^TTAAG
BlpI 2411 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2411 GCTCAGC GC^T[ACGT]AGC
BsaI 1831 GAGACC GGTCTC(1/5)
BsiHKAI 2000 GTGCTC G[AT]GC[AT]^C
BspHI 305 TCATGA T^CATGA
BspMI 735 GCAGGT ACCTGC(4/8)
BssSI 1565 CACGAG CACGAG(-5/-1)
CelII 2411 GCTCAGC GC^T[ACGT]AGC
DraIII 473 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 561 CCATGG C^C[GA][CT]GG
EaeI 1467 TGGCCA [CT]^GGCC[GA]
Eam1105I 287 GACAAGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 2152 GAATTC G^AATTC
EcoRV 2040 GATATC GAT^ATC
EcoT14I 561 CCATGG C^C[AT][AT]GG
EcoT22I 1351 ATGCAT ATGCA^T
FauI 21 GCGGG CCCGC(4/6)
FspI 627 TGCGCA TGC^GCA
HaeII 1370 AGCGCC [GA]GCGC^[CT]
MfeI 1046 CAATTG C^AATTG
MluNI 1467 TGGCCA TGG^CCA
MscI 1467 TGGCCA TGG^CCA
MunI 1046 CAATTG C^AATTG
NcoI 561 CCATGG C^CATGG
NsiI 1351 ATGCAT ATGCA^T
PleI 917 GAGTC GAGTC(4/5)
RcaI 305 TCATGA T^CATGA
SapI 1483 GAAGAGC GCTCTTC(1/4)
SpeI 838 ACTAGT A^CTAGT
SphI 1353 GCATGC GCATG^C
SspI 967 AATATT AAT^ATT
StuI 2207 AGGCCT AGG^CCT
StyI 561 CCATGG C^C[AT][AT]GG
TaqI 1605 TCGA T^CGA
TthHB8I 1605 TCGA T^CGA
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# TWO CUT ENZYMES ( 23 enzymes) #
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AlwNI 1369 CAGCGCCTG CAG[ACGT][ACGT][ACGT]^CTG
2006 CAGCTGCTG
AvaII 35 GGACC G^G[AT]CC
2327 GGACC
BanII 387 GGGCTC G[GA]GC[CT]^C
2409 GGGCTC
BbsI 121 GAAGAC GAAGAC(2/6)
999 GAAGAC
BglII 408 AGATCT A^GATCT
2424 AGATCT
BpmI 1675 CTCCAG CTGGAG(16/14)
2003 CTCCAG
BpuAI 121 GAAGAC GAAGAC(2/6)
999 GAAGAC
BsaBI 292 GATGTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
328 GATCTTAATC
BseRI 390 CTCCTC GAGGAG(10/8)
589 CTCCTC
BsgI 402 GTGCAG GTGCAG(16/14)
1795 GTGCAG
BsmI 1748 GAATGC GAATGC(1/-1)
2378 GAATGC
Bsp1407I 1005 TGTACA T^GTACA
1376 TGTACA
BsrGI 1005 TGTACA T^GTACA
1376 TGTACA
BstYI 408 AGATCT [GA]^GATC[CT]
2424 AGATCT
DraI 666 TTTAAA TTT^AAA
1186 TTTAAA
MamI 292 GATGTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
328 GATCTTAATC
MflI 408 AGATCT [GA]^GATC[CT]
2424 AGATCT
NlaIV 388 GGCTCC GG[ACGT]^[ACGT]CC
2326 GGGACC
PflMI 1241 CCAAACGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1843 CCACCCTCTGG
SspBI 1005 TGTACA T^GTACA
1376 TGTACA
Van91I 1241 CCAAACGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1843 CCACCCTCTGG
XcmI 926 CCATAAATATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1874 CCAATGATTTATTGG
XhoII 408 AGATCT [GA]^GATC[CT]
2424 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AccIII AciI AcsI AfaI AluI
AlwI ApoI BbvI BfaI BmyI
BsaJI BsaWI BseAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspEI BsrDI
BsrI BssKI BstAPI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DpnII
EarI EcoRII Fnu4HI FokI HaeIII
HapII HhaI HinP1I HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MnlI MroI
MseI MslI MspA1I MspI MvaI
MwoI NdeII NlaIII NspI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI