(Circular) (Six-base) MAPSORT of: pFN21AB5777.seq Check: 49 from: 1 to: 7171 pFN21AB5777 7171 bp With 182 enzymes: SgfI * April 15, 2010 09:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4981 278 Size: 53 83 186 936 3445 2468 Fragments arranged by size: 3445 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4514 1937 Size: 2577 4594 Acc65I G'GTAC_C Cuts at: 4492 4492 Size: 7171 AclI AA'CG_TT Cuts at: 1563 5298 5671 1563 Size: 3735 373 3063 Fragments arranged by size: 3735 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2088 5234 6618 6886 1632 Size: 456 3146 1384 268 1917 Fragments arranged by size: 3146 1917 1384 456 268 AflII C'TTAA_G Cuts at: 820 1017 2461 820 Size: 197 1444 5530 Fragments arranged by size: 5530 1444 197 AflIII A'CryG_T Cuts at: 1203 6070 7047 1203 Size: 4867 977 1327 Fragments arranged by size: 4867 1327 977 AgeI A'CCGG_T Cuts at: 6002 6002 Size: 7171 AhdI GACnn_n'nnGTC Cuts at: 2297 5900 2297 Size: 3603 3568 AleI CACnn'nnGTG Cuts at: 1166 3053 3563 1166 Size: 1887 510 4774 Fragments arranged by size: 4774 1887 510 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7171 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3285 3317 3285 Size: 32 7139 AlwNI CAG_nnn'CTG Cuts at: 3379 4016 4914 6486 3379 Size: 637 898 1572 4064 Fragments arranged by size: 4064 1572 898 637 ApaLI G'TGCA_C Cuts at: 5228 6384 5228 Size: 1156 6015 ApoI r'AATT_y Cuts at: 1493 2237 2545 2748 2766 3188 4157 4480 4706 Size: 744 308 203 18 422 969 323 226 Cuts at: 4706 4858 6967 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 969 744 422 323 308 226 203 152 18 AseI AT'TA_AT Cuts at: 160 1032 5725 160 Size: 872 4693 1606 Fragments arranged by size: 4693 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7171 AvaI C'yCGr_G Cuts at: 1870 1943 4496 1870 Size: 73 2553 4545 Fragments arranged by size: 4545 2553 73 BamHI G'GATC_C Cuts at: 4501 4868 4501 Size: 367 6804 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4492 5947 618 Size: 325 217 627 2705 1455 1842 Fragments arranged by size: 2705 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2396 4418 4490 1391 Size: 1005 2022 72 4072 Fragments arranged by size: 4072 2022 1005 72 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2133 3011 928 Size: 971 234 878 5088 Fragments arranged by size: 5088 971 878 234 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2335 2369 5362 5396 1903 Size: 34 50 34 314 34 2993 34 3678 Fragments arranged by size: 3678 2993 314 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5063 6273 5063 Size: 1210 5961 BclI T'GATC_A Cuts at: 1548 1548 Size: 7171 BfrBI ATG'CAT Cuts at: 3358 3358 Size: 7171 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5782 136 Size: 107 122 71 631 605 4110 1525 Fragments arranged by size: 4110 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2413 4429 7166 2413 Size: 2016 2737 2418 Fragments arranged by size: 2737 2418 2016 BlpI GC'TnA_GC Cuts at: 4417 4588 4417 Size: 171 7000 Bme1580I G_kGCm'C Cuts at: 1790 2655 5232 6388 1790 Size: 865 2577 1156 2573 Fragments arranged by size: 2577 2573 1156 865 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7171 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4182 4902 5860 Size: 416 53 83 399 24 2765 720 958 Cuts at: 5860 442 Size: 1753 Fragments arranged by size: 2765 1753 958 720 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7171 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3665 3993 5831 1239 Size: 2426 328 1838 2579 Fragments arranged by size: 2579 2426 1838 328 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2201 3767 5296 6161 6459 2201 Size: 1566 1529 865 298 2913 Fragments arranged by size: 2913 1566 1529 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3830 5834 882 Size: 617 2331 2004 2219 Fragments arranged by size: 2331 2219 2004 617 BsaAI yAC'GTr Cuts at: 493 1204 4962 493 Size: 711 3758 2702 Fragments arranged by size: 3758 2702 711 BsaBI GATnn'nnATC Cuts at: 2301 2337 7165 2301 Size: 36 4828 2307 Fragments arranged by size: 4828 2307 36 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4978 5360 5995 275 Size: 53 83 186 936 3445 382 635 1451 Fragments arranged by size: 3445 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2037 2046 2106 2690 5604 6002 6276 6423 6834 Size: 9 60 584 2914 398 274 147 411 Cuts at: 6834 2037 Size: 2374 Fragments arranged by size: 2914 2374 584 411 398 274 147 60 9 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3991 4021 1178 Size: 30 165 30 2588 30 4328 Fragments arranged by size: 4328 2588 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2386 2585 1186 Size: 1200 199 5772 Fragments arranged by size: 5772 1200 199 BseYI C'CCAG_C Cuts at: 2179 3982 4050 4315 6374 6870 2179 Size: 1803 68 265 2059 496 2480 Fragments arranged by size: 2480 2059 1803 496 265 68 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2428 3821 1163 Size: 573 692 1393 4513 Fragments arranged by size: 4513 1393 692 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4621 5382 6410 1540 Size: 275 186 2620 761 1028 2301 Fragments arranged by size: 2620 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4009 5232 5317 6388 4009 Size: 1223 85 1071 4792 Fragments arranged by size: 4792 1223 1071 85 BsmI GAATG_Cn' Cuts at: 3759 4389 4682 4775 3759 Size: 630 293 93 6155 Fragments arranged by size: 6155 630 293 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2396 2655 4009 4418 4490 5232 5317 Size: 399 606 259 1354 409 72 742 85 Cuts at: 5317 6388 1391 Size: 1071 2174 Fragments arranged by size: 2174 1354 1071 742 606 409 399 259 85 72 BspEI T'CCGG_A Cuts at: 2037 2046 2690 6834 2037 Size: 9 644 4144 2374 Fragments arranged by size: 4144 2374 644 9 BspHI T'CATG_A Cuts at: 2310 5060 6790 2310 Size: 2750 1730 2691 Fragments arranged by size: 2750 2691 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2731 4526 844 Size: 1887 1795 3489 Fragments arranged by size: 3489 1887 1795 BsrBI CCG'CTC Cuts at: 1394 1823 5058 1394 Size: 429 3235 3507 Fragments arranged by size: 3507 3235 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2277 3105 4428 5666 5840 65 Size: 1183 1029 828 1323 1238 174 1396 Fragments arranged by size: 1396 1323 1238 1183 1029 828 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5815 6002 1700 Size: 110 4005 187 2869 Fragments arranged by size: 4005 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3010 3381 96 Size: 2914 371 3886 Fragments arranged by size: 3886 2914 371 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7171 BssSI C'ACGA_G Cuts at: 3570 5231 6243 3570 Size: 1661 1012 4498 Fragments arranged by size: 4498 1661 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2483 2825 3203 4378 794 Size: 1689 342 378 1175 3587 Fragments arranged by size: 3587 1689 1175 378 342 BstBI TT'CG_AA Cuts at: 5975 5975 Size: 7171 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7171 BstYI r'GATC_y Cuts at: 1972 2413 4429 4501 4868 5253 5270 6722 6808 Size: 441 2016 72 367 385 17 1452 86 Cuts at: 6808 6820 7166 1972 Size: 12 346 1977 Fragments arranged by size: 2016 1977 1452 441 385 367 346 86 72 17 12 BtgI C'CryG_G Cuts at: 513 1065 2566 513 Size: 552 1501 5118 Fragments arranged by size: 5118 1501 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6432 Fragments arranged by size: 6432 683 56 BtsI GCAGTG_nn' Cuts at: 3585 4687 5481 5501 3585 Size: 1102 794 20 5255 Fragments arranged by size: 5255 1102 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7171 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7136 DraI TTT'AAA Cuts at: 2673 3193 4475 2673 Size: 520 1282 5369 Fragments arranged by size: 5369 1282 520 DraIII CAC_nnn'GTG Cuts at: 2483 2483 Size: 7171 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6178 809 Size: 507 4862 1802 Fragments arranged by size: 4862 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3472 4618 5507 Size: 54 1425 50 275 18 1642 1146 889 Cuts at: 5507 7136 8 Size: 1629 43 Fragments arranged by size: 1642 1629 1425 1146 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4618 1537 Size: 275 2806 4090 Fragments arranged by size: 4090 2806 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3477 3483 4097 5101 1353 Size: 2124 6 614 1004 3423 Fragments arranged by size: 3423 2124 1004 614 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5758 6132 6278 387 Size: 1064 138 4169 374 146 1280 Fragments arranged by size: 4169 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2088 3665 3993 5234 5831 6618 6886 Size: 393 456 1577 328 1241 597 787 268 Cuts at: 6886 1239 Size: 1524 Fragments arranged by size: 1577 1524 1241 787 597 456 393 328 268 EcoRI G'AATT_C Cuts at: 4157 4480 4858 4157 Size: 323 378 6470 Fragments arranged by size: 6470 378 323 EcoRV GAT'ATC Cuts at: 4047 4047 Size: 7171 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3176 3410 4162 3176 Size: 234 752 6185 Fragments arranged by size: 6185 752 234 FspI TGC'GCA Cuts at: 2634 5677 2634 Size: 3043 4128 HaeII r_GCGC'y Cuts at: 1678 3379 6318 1678 Size: 1701 2939 2531 Fragments arranged by size: 2939 2531 1701 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2067 2099 2283 2315 2888 Size: 32 135 32 495 32 184 32 573 Cuts at: 2888 2907 2920 2939 3191 3223 5812 5844 5886 Size: 19 13 19 252 32 2589 32 42 Cuts at: 5886 5918 1373 Size: 32 2626 Fragments arranged by size: 2626 2589 573 495 252 184 135 42 32 32 32 32 32 32 32 19 19 13 HincII GTy'rAC Cuts at: 669 1657 1938 4515 4761 669 Size: 988 281 2577 246 3079 Fragments arranged by size: 3079 2577 988 281 246 HindIII A'AGCT_T Cuts at: 748 2219 2645 3599 4394 748 Size: 1471 426 954 795 3525 Fragments arranged by size: 3525 1471 954 795 426 HpaI GTT'AAC Cuts at: 4761 4761 Size: 7171 KpnI G_GTAC'C Cuts at: 4496 4496 Size: 7171 MfeI C'AATT_G Cuts at: 3051 4770 3051 Size: 1719 5452 MluI A'CGCG_T Cuts at: 7047 7047 Size: 7171 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2126 2187 2475 3501 3953 4047 4242 6285 Size: 821 61 288 1026 452 94 195 2043 Cuts at: 6285 6469 1305 Size: 184 2007 Fragments arranged by size: 2043 2007 1026 821 452 288 195 184 94 61 MscI TGG'CCA Cuts at: 10 64 1489 1832 3474 10 Size: 54 1425 343 1642 3707 Fragments arranged by size: 3707 1642 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2140 2603 3053 3269 3563 3671 5129 Size: 648 974 463 450 216 294 108 1458 Cuts at: 5129 5488 5647 518 Size: 359 159 2042 Fragments arranged by size: 2042 1458 974 648 463 450 359 294 216 159 108 MspA1I CmG'CkG Cuts at: 1606 4013 4282 4378 4587 5264 6412 6657 1606 Size: 2407 269 96 209 677 1148 245 2120 Fragments arranged by size: 2407 2120 1148 677 269 245 209 96 NcoI C'CATG_G Cuts at: 513 1065 2566 513 Size: 552 1501 5118 Fragments arranged by size: 5118 1501 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7171 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7171 NotI GC'GGCC_GC Cuts at: 4618 4618 Size: 7171 NsiI A_TGCA'T Cuts at: 3360 3360 Size: 7171 NspI r_CATG'y Cuts at: 1132 1229 2179 2629 3362 4529 6074 1132 Size: 97 950 450 733 1167 1545 2229 Fragments arranged by size: 2229 1545 1167 950 733 450 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7171 PciI A'CATG_T Cuts at: 6070 6070 Size: 7171 PflMI CCAn_nnn'nTGG Cuts at: 1108 3252 3854 1108 Size: 2144 602 4425 Fragments arranged by size: 4425 2144 602 PmeI GTTT'AAAC Cuts at: 4475 4475 Size: 7171 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5240 5272 6753 6785 5240 Size: 32 1481 32 5626 Fragments arranged by size: 5626 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7171 PsiI TTA'TAA Cuts at: 4741 4741 Size: 7171 PstI C_TGCA'G Cuts at: 830 3229 4111 4264 4287 4523 830 Size: 2399 882 153 23 236 3478 Fragments arranged by size: 3478 2399 882 236 153 23 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7171 PvuII CAG'CTG Cuts at: 4013 4282 4378 4013 Size: 269 96 6806 Fragments arranged by size: 6806 269 96 SalI G'TCGA_C Cuts at: 1936 4513 1936 Size: 2577 4594 SapI GCTCTTCn'nnn_ Cuts at: 3483 3483 Size: 7171 SbfI CC_TGCA'GG Cuts at: 4523 4523 Size: 7171 ScaI AGT'ACT Cuts at: 5419 7131 5419 Size: 1712 5459 SfcI C'TryA_G Cuts at: 826 1045 2586 3225 4107 4260 4283 4519 5654 Size: 219 1541 639 882 153 23 236 1135 Cuts at: 5654 6335 6526 826 Size: 681 191 1471 Fragments arranged by size: 1541 1471 1135 882 681 639 236 219 191 153 23 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7171 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7171 SmaI CCC'GGG Cuts at: 1872 4498 1872 Size: 2626 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2216 2461 3746 5275 6176 6438 Size: 197 926 273 245 1285 1529 901 262 Cuts at: 6438 820 Size: 1553 Fragments arranged by size: 1553 1529 1285 926 901 273 262 245 197 SnaBI TAC'GTA Cuts at: 493 4962 493 Size: 4469 2702 SpeI A'CTAG_T Cuts at: 152 2843 152 Size: 2691 4480 SphI G_CATG'C Cuts at: 1132 3362 4529 1132 Size: 2230 1167 3774 Fragments arranged by size: 3774 2230 1167 SspI AAT'ATT Cuts at: 5 52 2974 5095 5 Size: 47 2922 2121 2081 Fragments arranged by size: 2922 2121 2081 47 StuI AGG'CCT Cuts at: 1517 4214 1517 Size: 2697 4474 StyI C'CwwG_G Cuts at: 513 1065 2566 4610 513 Size: 552 1501 2044 3074 Fragments arranged by size: 3074 2044 1501 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5198 5215 5368 5553 5998 325 Size: 1255 3618 17 153 185 445 1498 Fragments arranged by size: 3618 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3010 3031 3381 Size: 274 80 33 51 1443 1033 21 350 Cuts at: 3381 3620 5417 7129 96 Size: 239 1797 1712 138 Fragments arranged by size: 1797 1712 1443 1033 350 274 239 138 80 51 33 21 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7131 7163 7131 Size: 32 7139 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7171 XbaI T'CTAG_A Cuts at: 4507 4507 Size: 7171 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2938 3886 2938 Size: 948 6223 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7171 XmaI C'CCGG_G Cuts at: 1870 4496 1870 Size: 2626 4545 XmnI GAAnn'nnTTC Cuts at: 5300 5300 Size: 7171 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4979 276 Size: 53 83 186 936 3445 2468 Fragments arranged by size: 3445 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI